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P22300 (GBRG2_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 115. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Gamma-aminobutyric acid receptor subunit gamma-2
Alternative name(s):
GABA(A) receptor subunit gamma-2
Gene names
Name:GABRG2
OrganismBos taurus (Bovine) [Reference proteome]
Taxonomic identifier9913 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos

Protein attributes

Sequence length475 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel.

Subunit structure

Generally pentameric. There are five types of GABA(A) receptor chains: alpha, beta, gamma, delta, and rho.

Subcellular location

Cell junctionsynapsepostsynaptic cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein.

Post-translational modification

Palmitoylated by ZDHHC3/GODZ; which may affect presynaptic clustering and/or cell surface stability By similarity.

Miscellaneous

This subunit carries the benzodiazepine binding site.

Sequence similarities

Belongs to the ligand-gated ion channel (TC 1.A.9) family. Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily. GABRG2 sub-subfamily. [View classification]

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 2L (identifier: P22300-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2S (identifier: P22300-2)

The sequence of this isoform differs from the canonical sequence as follows:
     377-384: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3939 Potential
Chain40 – 475436Gamma-aminobutyric acid receptor subunit gamma-2
PRO_0000000476

Regions

Topological domain40 – 273234Extracellular Probable
Transmembrane274 – 29623Helical; Probable
Transmembrane300 – 32223Helical; Probable
Transmembrane334 – 35623Helical; Probable
Topological domain357 – 45195Cytoplasmic Probable
Transmembrane452 – 47524Helical; Probable

Amino acid modifications

Modified residue3821Phosphoserine; by PKC Probable
Glycosylation521N-linked (GlcNAc...) Potential
Glycosylation1291N-linked (GlcNAc...) Potential
Glycosylation2471N-linked (GlcNAc...) Potential
Disulfide bond190 ↔ 204 By similarity

Natural variations

Alternative sequence377 – 3848Missing in isoform 2S.
VSP_000090
Natural variant2301S → G.

Sequences

Sequence LengthMass (Da)Tools
Isoform 2L [UniParc].

Last modified August 1, 1991. Version 1.
Checksum: A881082D3990197F

FASTA47555,265
        10         20         30         40         50         60 
MSSPNIWSTG SSVYSTPVFS QKMTLWILLL LSLYPGLTRQ KSDDDYEDYA SNKTWVLTPK 

        70         80         90        100        110        120 
VPEGDVTVIL NNLLEGYDNK LRPDIGVKPT LIHTDMYVNS IGPVNAINME YTIDIFFGQT 

       130        140        150        160        170        180 
WYDRRLKFNS TIKVLRLNSN MVGKIWIPDT FFRNSKKADA HWITTPNRML RIWNDGRVLY 

       190        200        210        220        230        240 
TLRLTIDAEC QLQLHNFPMD EHSCPLEFSS YGYPREEIVY QWKRSSVEVS DTRSWRLYQF 

       250        260        270        280        290        300 
SFVGLRNTTE VVKTTSGDYV VMTVYFDLSR RMGYFTIQTY IPCTLIVVLS WVSFWINKDA 

       310        320        330        340        350        360 
VPARTSLGIT TVLTMTTLST IARKSLPKVS YVTAMDLFVS VCFIFVFSAL VEYGTLHYFV 

       370        380        390        400        410        420 
SNRKPSKDKD KKKKNPLLRM FSFKAPTIDI RPRSATIQMN NATHLQERDE EYGYECLDGK 

       430        440        450        460        470 
DCASFFCCFE DCRTGAWRHG RIHIRIAKMD SYARIFFPTA FCLFNLVYWV SYLYL 

« Hide

Isoform 2S [UniParc].

Checksum: 456EDACE25CCD0B6
Show »

FASTA46754,241

References

[1]"Another mechanism for creating diversity in gamma-aminobutyrate type A receptors: RNA splicing directs expression of two forms of gamma 2 phosphorylation site."
Whiting P.J., McKernan R.M., Iversen L.L.
Proc. Natl. Acad. Sci. U.S.A. 87:9966-9970(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 2L AND 2S).
Tissue: Brain cortex.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M55563 mRNA. Translation: AAA30531.1.
PIRB39272.
RefSeqNP_776970.1. NM_174545.1. [P22300-1]
UniGeneBt.408.

3D structure databases

ProteinModelPortalP22300.
SMRP22300. Positions 301-358.
ModBaseSearch...
MobiDBSearch...

Chemistry

BindingDBP22300.
ChEMBLCHEMBL2094107.

Proteomic databases

PRIDEP22300.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID282240.
KEGGbta:282240.

Organism-specific databases

CTD2566.

Phylogenomic databases

eggNOGNOG238757.
HOGENOMHOG000231337.
HOVERGENHBG051707.
InParanoidP22300.
KOK05186.

Family and domain databases

Gene3D2.70.170.10. 1 hit.
InterProIPR006028. GABAA/Glycine_rcpt.
IPR005439. GABBAg2_rcpt.
IPR005437. GABBAg_rcpt.
IPR006202. Neur_chan_lig-bd.
IPR006201. Neur_channel.
IPR006029. Neurotrans-gated_channel_TM.
IPR018000. Neurotransmitter_ion_chnl_CS.
[Graphical view]
PANTHERPTHR18945. PTHR18945. 1 hit.
PfamPF02931. Neur_chan_LBD. 1 hit.
PF02932. Neur_chan_memb. 1 hit.
[Graphical view]
PRINTSPR00253. GABAARECEPTR.
PR01620. GABAARGAMMA.
PR01622. GABAARGAMMA2.
PR00252. NRIONCHANNEL.
SUPFAMSSF63712. SSF63712. 1 hit.
SSF90112. SSF90112. 1 hit.
TIGRFAMsTIGR00860. LIC. 1 hit.
PROSITEPS00236. NEUROTR_ION_CHANNEL. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20806059.

Entry information

Entry nameGBRG2_BOVIN
AccessionPrimary (citable) accession number: P22300
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: August 1, 1991
Last modified: May 14, 2014
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families