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P22300

- GBRG2_BOVIN

UniProt

P22300 - GBRG2_BOVIN

Protein

Gamma-aminobutyric acid receptor subunit gamma-2

Gene

GABRG2

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 116 (01 Oct 2014)
      Sequence version 1 (01 Aug 1991)
      Previous versions | rss
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    Functioni

    Component of the heteropentameric receptor for GABA, the major inhibitory neurotransmitter in the vertebrate brain. Functions also as histamine receptor and mediates cellular responses to histamine. Functions as receptor for diazepines and various anesthetics, such as pentobarbital; these are bound at a separate allosteric effector binding site. Functions as ligand-gated chloride channel By similarity.By similarity

    GO - Molecular functioni

    1. chloride channel activity Source: UniProtKB
    2. extracellular ligand-gated ion channel activity Source: InterPro
    3. GABA-A receptor activity Source: UniProtKB

    GO - Biological processi

    1. cellular response to histamine Source: UniProtKB
    2. chloride transmembrane transport Source: UniProtKB
    3. gamma-aminobutyric acid signaling pathway Source: InterPro

    Keywords - Molecular functioni

    Chloride channel, Ion channel

    Keywords - Biological processi

    Ion transport, Transport

    Keywords - Ligandi

    Chloride

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Gamma-aminobutyric acid receptor subunit gamma-2
    Alternative name(s):
    GABA(A) receptor subunit gamma-2
    Gene namesi
    Name:GABRG2
    OrganismiBos taurus (Bovine)
    Taxonomic identifieri9913 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
    ProteomesiUP000009136: Unplaced

    Subcellular locationi

    GO - Cellular componenti

    1. cell junction Source: UniProtKB-KW
    2. chloride channel complex Source: UniProtKB-KW
    3. GABA-A receptor complex Source: UniProtKB
    4. integral component of plasma membrane Source: UniProtKB
    5. postsynaptic membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 3939Sequence AnalysisAdd
    BLAST
    Chaini40 – 475436Gamma-aminobutyric acid receptor subunit gamma-2PRO_0000000476Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi52 – 521N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi129 – 1291N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi190 ↔ 204By similarity
    Glycosylationi247 – 2471N-linked (GlcNAc...)Sequence Analysis
    Modified residuei382 – 3821Phosphoserine; by PKCCurated

    Post-translational modificationi

    Palmitoylated by ZDHHC3/GODZ; which may affect presynaptic clustering and/or cell surface stability.By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Lipoprotein, Palmitate, Phosphoprotein

    Proteomic databases

    PRIDEiP22300.

    Interactioni

    Subunit structurei

    Heteropentamer, formed by a combination of alpha, beta, gamma, delta and rho chains. Interacts with GABARAP By similarity.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliP22300.
    SMRiP22300. Positions 301-358.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini40 – 273234ExtracellularCuratedAdd
    BLAST
    Topological domaini357 – 45195CytoplasmicCuratedAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei274 – 29623HelicalCuratedAdd
    BLAST
    Transmembranei300 – 32223HelicalCuratedAdd
    BLAST
    Transmembranei334 – 35623HelicalCuratedAdd
    BLAST
    Transmembranei452 – 47524HelicalCuratedAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG238757.
    HOGENOMiHOG000231337.
    HOVERGENiHBG051707.
    InParanoidiP22300.
    KOiK05186.

    Family and domain databases

    Gene3Di2.70.170.10. 1 hit.
    InterProiIPR006028. GABAA/Glycine_rcpt.
    IPR005439. GABBAg2_rcpt.
    IPR005437. GABBAg_rcpt.
    IPR006202. Neur_chan_lig-bd.
    IPR006201. Neur_channel.
    IPR006029. Neurotrans-gated_channel_TM.
    IPR018000. Neurotransmitter_ion_chnl_CS.
    [Graphical view]
    PANTHERiPTHR18945. PTHR18945. 1 hit.
    PfamiPF02931. Neur_chan_LBD. 1 hit.
    PF02932. Neur_chan_memb. 1 hit.
    [Graphical view]
    PRINTSiPR00253. GABAARECEPTR.
    PR01620. GABAARGAMMA.
    PR01622. GABAARGAMMA2.
    PR00252. NRIONCHANNEL.
    SUPFAMiSSF63712. SSF63712. 1 hit.
    SSF90112. SSF90112. 1 hit.
    TIGRFAMsiTIGR00860. LIC. 1 hit.
    PROSITEiPS00236. NEUROTR_ION_CHANNEL. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 2L (identifier: P22300-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MSSPNIWSTG SSVYSTPVFS QKMTLWILLL LSLYPGLTRQ KSDDDYEDYA    50
    SNKTWVLTPK VPEGDVTVIL NNLLEGYDNK LRPDIGVKPT LIHTDMYVNS 100
    IGPVNAINME YTIDIFFGQT WYDRRLKFNS TIKVLRLNSN MVGKIWIPDT 150
    FFRNSKKADA HWITTPNRML RIWNDGRVLY TLRLTIDAEC QLQLHNFPMD 200
    EHSCPLEFSS YGYPREEIVY QWKRSSVEVS DTRSWRLYQF SFVGLRNTTE 250
    VVKTTSGDYV VMTVYFDLSR RMGYFTIQTY IPCTLIVVLS WVSFWINKDA 300
    VPARTSLGIT TVLTMTTLST IARKSLPKVS YVTAMDLFVS VCFIFVFSAL 350
    VEYGTLHYFV SNRKPSKDKD KKKKNPLLRM FSFKAPTIDI RPRSATIQMN 400
    NATHLQERDE EYGYECLDGK DCASFFCCFE DCRTGAWRHG RIHIRIAKMD 450
    SYARIFFPTA FCLFNLVYWV SYLYL 475
    Length:475
    Mass (Da):55,265
    Last modified:August 1, 1991 - v1
    Checksum:iA881082D3990197F
    GO
    Isoform 2S (identifier: P22300-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         377-384: Missing.

    Show »
    Length:467
    Mass (Da):54,241
    Checksum:i456EDACE25CCD0B6
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti230 – 2301S → G.

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei377 – 3848Missing in isoform 2S. 1 PublicationVSP_000090

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M55563 mRNA. Translation: AAA30531.1.
    PIRiB39272.
    RefSeqiNP_776970.1. NM_174545.1. [P22300-1]
    UniGeneiBt.408.

    Genome annotation databases

    GeneIDi282240.
    KEGGibta:282240.

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M55563 mRNA. Translation: AAA30531.1 .
    PIRi B39272.
    RefSeqi NP_776970.1. NM_174545.1. [P22300-1 ]
    UniGenei Bt.408.

    3D structure databases

    ProteinModelPortali P22300.
    SMRi P22300. Positions 301-358.
    ModBasei Search...
    MobiDBi Search...

    Chemistry

    BindingDBi P22300.
    ChEMBLi CHEMBL2094107.

    Proteomic databases

    PRIDEi P22300.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 282240.
    KEGGi bta:282240.

    Organism-specific databases

    CTDi 2566.

    Phylogenomic databases

    eggNOGi NOG238757.
    HOGENOMi HOG000231337.
    HOVERGENi HBG051707.
    InParanoidi P22300.
    KOi K05186.

    Miscellaneous databases

    NextBioi 20806059.

    Family and domain databases

    Gene3Di 2.70.170.10. 1 hit.
    InterProi IPR006028. GABAA/Glycine_rcpt.
    IPR005439. GABBAg2_rcpt.
    IPR005437. GABBAg_rcpt.
    IPR006202. Neur_chan_lig-bd.
    IPR006201. Neur_channel.
    IPR006029. Neurotrans-gated_channel_TM.
    IPR018000. Neurotransmitter_ion_chnl_CS.
    [Graphical view ]
    PANTHERi PTHR18945. PTHR18945. 1 hit.
    Pfami PF02931. Neur_chan_LBD. 1 hit.
    PF02932. Neur_chan_memb. 1 hit.
    [Graphical view ]
    PRINTSi PR00253. GABAARECEPTR.
    PR01620. GABAARGAMMA.
    PR01622. GABAARGAMMA2.
    PR00252. NRIONCHANNEL.
    SUPFAMi SSF63712. SSF63712. 1 hit.
    SSF90112. SSF90112. 1 hit.
    TIGRFAMsi TIGR00860. LIC. 1 hit.
    PROSITEi PS00236. NEUROTR_ION_CHANNEL. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Another mechanism for creating diversity in gamma-aminobutyrate type A receptors: RNA splicing directs expression of two forms of gamma 2 phosphorylation site."
      Whiting P.J., McKernan R.M., Iversen L.L.
      Proc. Natl. Acad. Sci. U.S.A. 87:9966-9970(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 2L AND 2S).
      Tissue: Brain cortex.

    Entry informationi

    Entry nameiGBRG2_BOVIN
    AccessioniPrimary (citable) accession number: P22300
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1991
    Last sequence update: August 1, 1991
    Last modified: October 1, 2014
    This is version 116 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    This subunit carries the benzodiazepine binding site.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3