P22289 (QCR9_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cytochrome b-c1 complex subunit 9

Alternative name(s):
Complex III subunit 9
Complex III subunit X
Cytochrome c1 non-heme 7.3 kDa protein
Ubiquinol-cytochrome c reductase complex 7.3 kDa protein

Gene names

Name:	QCR9
Synonyms:	UCR9
Ordered Locus Names:	YGR183C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	66 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is part of the mitochondrial respiratory chain that generates an electrochemical potential coupled to ATP synthesis. The complex couples electron transfer from ubiquinol to cytochrome c. QCR9 is required for formation of a fully functional complex.
Subunit structure	Fungal cytochrome b-c1 complex contains 10 subunits; 3 respiratory subunits, 2 core proteins and 5 low-molecular weight proteins. Cytochrome b-c1 complex is a homodimer.
Subcellular location	Mitochondrion inner membrane.
Miscellaneous	Present with 4550 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the UQCR10/QCR9 family.

Ontologies

Keywords
Biological process	Electron transport Respiratory chain Transport
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Domain	Transmembrane Transmembrane helix
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	aerobic respiration Inferred from mutant phenotype PubMed 1332881. Source: SGD iron-sulfur cluster assembly Inferred from mutant phenotype PubMed 8389362. Source: SGD mitochondrial electron transport, ubiquinol to cytochrome c Inferred from mutant phenotype PubMed 1332881. Source: SGD
Cellular_component	integral to membrane Inferred from sequence model PubMed 12192589. Source: SGD mitochondrial respiratory chain complex III Inferred from direct assay Ref.6. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ALO1	P54783	1	EBI-19947,EBI-2519
PEX11	Q12462	1	EBI-19947,EBI-13198

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed Ref.1

Chain

2 – 66

65

Cytochrome b-c1 complex subunit 9

PRO_0000193558

Regions

Transmembrane

16 – 37

22

Helical; Potential

Secondary structure

1

.

.

.

.

.

.

.

.

66

Helix Strand Turn

Details...

Helix

4 – 11

8

Helix

18 – 43

26

Turn

44 – 47

4

Helix

49 – 55

7

Sequences

	Sequence		Length	Mass (Da)
	P22289 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 120106C00F3CCED0	FASTA	66	7,476
	10 20 30 40 50 60 MSFSSLYKTF FKRNAVFVGT IFAGAFVFQT VFDTAITSWY ENHNKGKLWK DVKARIAAGD GDDDDE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation and characterization of QCR9, a nuclear gene encoding the 7.3-kDa subunit 9 of the Saccharomyces cerevisiae ubiquinol-cytochrome c oxidoreductase complex. An intron-containing gene with a conserved sequence occurring in the intron of COX4." Phillips J.D., Schmitt M.E., Brown T.A., Beckmann J.D., Trumpower B.L. J. Biol. Chem. 265:20813-20821(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-10. Strain: ATCC 64665 / S288c / DC5.
[2]	"DNA sequence analysis of a 23,002 bp DNA fragment of the right arm of Saccharomyces cerevisiae chromosome VII." Arroyo J., Garcia-Gonzalez M., Garcia-Saez M.I., Sanchez-Perez M., Nombela C. Yeast 13:357-363(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K. Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"Structure at 2.3 A resolution of the cytochrome bc1 complex from the yeast Saccharomyces cerevisiae co-crystallized with an antibody Fv fragment." Hunte C., Koepke J., Lange C., Rossmanith T., Michel H. Structure 8:669-684(2000) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS).
[7]	"Crystal structure of the yeast cytochrome bc1 complex with its bound substrate cytochrome c." Lange C., Hunte C. Proc. Natl. Acad. Sci. U.S.A. 99:2800-2805(2002) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.97 ANGSTROMS).
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M59797 Genomic DNA. Translation: AAA63575.1.
Z72968 Genomic DNA. Translation: CAA97209.1.
BK006941 Genomic DNA. Translation: DAA08277.1.

PIR

A38325.

RefSeq

NP_011699.1. NM_001181312.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1EZV	X-ray	2.30	I	4-58	[»]
1KB9	X-ray	2.30	I	4-58	[»]
1KYO	X-ray	2.97	I/T	2-58	[»]
1P84	X-ray	2.50	I	4-57	[»]
2IBZ	X-ray	2.30	I	1-66	[»]
3CX5	X-ray	1.90	I/T	2-66	[»]
3CXH	X-ray	2.50	I/T	2-66	[»]

ProteinModelPortal

P22289.

SMR

P22289. Positions 2-58.

ModBase

Search...

Protein-protein interaction databases

IntAct

P22289. 5 interactions.

MINT

MINT-4487623.

STRING

4932.YGR183C.

Proteomic databases

PaxDb

P22289.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YGR183C; YGR183C; YGR183C.

GeneID

853095.

KEGG

sce:YGR183C.

Organism-specific databases

SGD

S000003415. QCR9.

Phylogenomic databases

eggNOG

NOG274563.

HOGENOM

HOG000215187.

KO

K00419.

OMA

KDIRHKY.

OrthoDB

EOG486CPC.

Gene expression databases

Genevestigator

P22289.

GermOnline

YGR183C. Saccharomyces cerevisiae.

Family and domain databases

Gene3D

1.20.5.260. 1 hit.

InterPro

IPR008027. QCR9.
[Graphical view]

PANTHER

PTHR12980. PTHR12980. 1 hit.

Pfam

PF05365. UCR_UQCRX_QCR9. 1 hit.
[Graphical view]

SUPFAM

SSF81514. Ubiquinol_cytC_Reductase_QCR9. 1 hit.

ProtoNet

Search...

Other

EvolutionaryTrace

P22289.

NextBio

973084.

Entry information

Entry name

QCR9_YEAST

Accession

Primary (citable) accession number: P22289
Secondary accession number(s): D6VUW6

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1991
Last sequence update:	January 23, 2007
Last modified:	May 1, 2013
This is version 128 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program