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Protein

DNA-directed RNA polymerase III subunit RPC2

Gene

RET1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Second largest core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. Pol III is composed of mobile elements and RPC2 is part of the core element with the central large cleft and probably a clamp element that moves to open and close the cleft (By similarity).By similarity

Miscellaneous

Present with 1950 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi1095ZincBy similarity1
Metal bindingi1098ZincBy similarity1
Metal bindingi1107ZincBy similarity1
Metal bindingi1110ZincBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri1095 – 1110C4-typeBy similarityAdd BLAST16

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionNucleotidyltransferase, Transferase
Biological processTranscription
LigandMetal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-33711-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-directed RNA polymerase III subunit RPC2 (EC:2.7.7.6)
Short name:
RNA polymerase III subunit C2
Alternative name(s):
C128
DNA-directed RNA polymerase III 130 kDa polypeptide
Gene namesi
Name:RET1
Synonyms:RPC128, RPC2
Ordered Locus Names:YOR207C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOR207C
SGDiS000005733 RET1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

DNA-directed RNA polymerase, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000480961 – 1149DNA-directed RNA polymerase III subunit RPC2Add BLAST1149

Proteomic databases

MaxQBiP22276
PaxDbiP22276
PRIDEiP22276

PTM databases

CarbonylDBiP22276
iPTMnetiP22276

Interactioni

Subunit structurei

Component of the RNA polymerase III (Pol III) complex consisting of 17 subunits.

Protein-protein interaction databases

BioGridi34602, 80 interactors
DIPiDIP-997N
IntActiP22276, 29 interactors
MINTiP22276
STRINGi4932.YOR207C

Structurei

Secondary structure

11149
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi40 – 42Combined sources3
Helixi43 – 46Combined sources4
Helixi47 – 54Combined sources8
Helixi59 – 68Combined sources10
Turni69 – 71Combined sources3
Helixi72 – 76Combined sources5
Beta strandi86 – 89Combined sources4
Beta strandi93 – 101Combined sources9
Beta strandi104 – 106Combined sources3
Helixi117 – 120Combined sources4
Beta strandi126 – 135Combined sources10
Beta strandi137 – 139Combined sources3
Beta strandi143 – 153Combined sources11
Beta strandi158 – 162Combined sources5
Helixi168 – 173Combined sources6
Beta strandi185 – 187Combined sources3
Beta strandi190 – 194Combined sources5
Beta strandi196 – 200Combined sources5
Beta strandi204 – 208Combined sources5
Beta strandi211 – 213Combined sources3
Beta strandi216 – 221Combined sources6
Beta strandi236 – 238Combined sources3
Beta strandi240 – 243Combined sources4
Beta strandi247 – 249Combined sources3
Helixi253 – 259Combined sources7
Helixi266 – 269Combined sources4
Turni271 – 273Combined sources3
Beta strandi275 – 279Combined sources5
Helixi280 – 288Combined sources9
Turni289 – 292Combined sources4
Turni297 – 299Combined sources3
Helixi301 – 307Combined sources7
Beta strandi309 – 311Combined sources3
Turni318 – 332Combined sources15
Helixi344 – 361Combined sources18
Helixi372 – 374Combined sources3
Beta strandi375 – 378Combined sources4
Helixi380 – 403Combined sources24
Turni404 – 408Combined sources5
Beta strandi409 – 412Combined sources4
Beta strandi416 – 419Combined sources4
Turni423 – 425Combined sources3
Helixi428 – 439Combined sources12
Turni445 – 447Combined sources3
Beta strandi455 – 457Combined sources3
Helixi463 – 468Combined sources6
Beta strandi472 – 475Combined sources4
Beta strandi480 – 482Combined sources3
Turni485 – 487Combined sources3
Helixi491 – 493Combined sources3
Turni494 – 496Combined sources3
Beta strandi508 – 514Combined sources7
Helixi527 – 535Combined sources9
Turni541 – 543Combined sources3
Beta strandi550 – 552Combined sources3
Beta strandi554 – 557Combined sources4
Helixi568 – 579Combined sources12
Turni580 – 582Combined sources3
Beta strandi589 – 593Combined sources5
Turni594 – 597Combined sources4
Beta strandi598 – 602Combined sources5
Beta strandi608 – 615Combined sources8
Beta strandi617 – 621Combined sources5
Helixi624 – 630Combined sources7
Beta strandi632 – 634Combined sources3
Helixi639 – 642Combined sources4
Beta strandi645 – 650Combined sources6
Turni651 – 653Combined sources3
Helixi654 – 656Combined sources3
Beta strandi659 – 663Combined sources5
Helixi677 – 680Combined sources4
Helixi685 – 687Combined sources3
Beta strandi688 – 690Combined sources3
Turni691 – 693Combined sources3
Helixi697 – 705Combined sources9
Turni706 – 708Combined sources3
Turni715 – 718Combined sources4
Beta strandi723 – 730Combined sources8
Beta strandi735 – 737Combined sources3
Turni739 – 748Combined sources10
Beta strandi752 – 760Combined sources9
Beta strandi767 – 774Combined sources8
Turni775 – 781Combined sources7
Beta strandi785 – 795Combined sources11
Turni799 – 801Combined sources3
Beta strandi815 – 817Combined sources3
Turni819 – 823Combined sources5
Beta strandi826 – 830Combined sources5
Beta strandi841 – 843Combined sources3
Beta strandi845 – 850Combined sources6
Beta strandi859 – 861Combined sources3
Beta strandi866 – 868Combined sources3
Beta strandi879 – 887Combined sources9
Helixi889 – 892Combined sources4
Beta strandi894 – 904Combined sources11
Beta strandi911 – 913Combined sources3
Beta strandi915 – 917Combined sources3
Beta strandi920 – 926Combined sources7
Beta strandi928 – 931Combined sources4
Turni934 – 936Combined sources3
Beta strandi941 – 944Combined sources4
Turni946 – 952Combined sources7
Helixi956 – 968Combined sources13
Turni969 – 971Combined sources3
Turni985 – 987Combined sources3
Helixi988 – 992Combined sources5
Turni993 – 995Combined sources3
Beta strandi998 – 1002Combined sources5
Beta strandi1007 – 1009Combined sources3
Beta strandi1017 – 1026Combined sources10
Turni1032 – 1034Combined sources3
Beta strandi1036 – 1038Combined sources3
Beta strandi1040 – 1043Combined sources4
Beta strandi1045 – 1047Combined sources3
Beta strandi1053 – 1056Combined sources4
Beta strandi1060 – 1062Combined sources3
Helixi1064 – 1073Combined sources10
Helixi1076 – 1083Combined sources8
Helixi1085 – 1088Combined sources4
Beta strandi1089 – 1096Combined sources8
Beta strandi1103 – 1105Combined sources3
Beta strandi1108 – 1110Combined sources3
Beta strandi1116 – 1122Combined sources7
Helixi1123 – 1134Combined sources12
Beta strandi1138 – 1146Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5FJ8electron microscopy3.90B1-1149[»]
5FJ9electron microscopy4.60B1-1149[»]
5FJAelectron microscopy4.65B1-1149[»]
6EU0electron microscopy4.00B1-1149[»]
6EU1electron microscopy3.40B1-1149[»]
6EU2electron microscopy3.40B1-1149[»]
6EU3electron microscopy3.30B1-1149[»]
6F40electron microscopy3.70B1-1149[»]
6F41electron microscopy4.30B1-1149[»]
6F42electron microscopy5.50B1-1149[»]
6F44electron microscopy4.20B1-1149[»]
ProteinModelPortaliP22276
SMRiP22276
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the RNA polymerase beta chain family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri1095 – 1110C4-typeBy similarityAdd BLAST16

Keywords - Domaini

Zinc-finger

Phylogenomic databases

GeneTreeiENSGT00910000144264
HOGENOMiHOG000222962
InParanoidiP22276
KOiK03021
OMAiMLCTSDT
OrthoDBiEOG092C06IY

Family and domain databases

CDDicd00653 RNA_pol_B_RPB2, 1 hit
Gene3Di2.40.270.10, 3 hits
2.40.50.150, 2 hits
3.90.1110.10, 1 hit
InterProiView protein in InterPro
IPR015712 DNA-dir_RNA_pol_su2
IPR007120 DNA-dir_RNAP_su2_dom
IPR037033 DNA-dir_RNAP_su2_hyb_sf
IPR007121 RNA_pol_bsu_CS
IPR007644 RNA_pol_bsu_protrusion
IPR007642 RNA_pol_Rpb2_2
IPR037034 RNA_pol_Rpb2_2_sf
IPR007645 RNA_pol_Rpb2_3
IPR007646 RNA_pol_Rpb2_4
IPR007647 RNA_pol_Rpb2_5
IPR007641 RNA_pol_Rpb2_7
IPR014724 RNA_pol_RPB2_OB-fold
PANTHERiPTHR20856 PTHR20856, 1 hit
PfamiView protein in Pfam
PF04563 RNA_pol_Rpb2_1, 1 hit
PF04561 RNA_pol_Rpb2_2, 1 hit
PF04565 RNA_pol_Rpb2_3, 1 hit
PF04566 RNA_pol_Rpb2_4, 1 hit
PF04567 RNA_pol_Rpb2_5, 1 hit
PF00562 RNA_pol_Rpb2_6, 1 hit
PF04560 RNA_pol_Rpb2_7, 1 hit
PROSITEiView protein in PROSITE
PS01166 RNA_POL_BETA, 1 hit

Sequencei

Sequence statusi: Complete.

P22276-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVAATKRRKT HIHKHVKDEA FDDLLKPVYK GKKLTDEINT AQDKWHLLPA
60 70 80 90 100
FLKVKGLVKQ HLDSFNYFVD TDLKKIIKAN QLILSDVDPE FYLKYVDIRV
110 120 130 140 150
GKKSSSSTKD YLTPPHECRL RDMTYSAPIY VDIEYTRGRN IIMHKDVEIG
160 170 180 190 200
RMPIMLRSNK CILYDADESK MAKLNECPLD PGGYFIVNGT EKVILVQEQL
210 220 230 240 250
SKNRIIVEAD EKKGIVQASV TSSTHERKSK TYVITKNGKI YLKHNSIAEE
260 270 280 290 300
IPIAIVLKAC GILSDLEIMQ LVCGNDSSYQ DIFAVNLEES SKLDIYTQQQ
310 320 330 340 350
ALEYIGAKVK TMRRQKLTIL QEGIEAIATT VIAHLTVEAL DFREKALYIA
360 370 380 390 400
MMTRRVVMAM YNPKMIDDRD YVGNKRLELA GQLISLLFED LFKKFNNDFK
410 420 430 440 450
LSIDKVLKKP NRAMEYDALL SINVHSNNIT SGLNRAISTG NWSLKRFKME
460 470 480 490 500
RAGVTHVLSR LSYISALGMM TRISSQFEKS RKVSGPRALQ PSQFGMLCTA
510 520 530 540 550
DTPEGEACGL VKNLALMTHI TTDDEEEPIK KLCYVLGVED ITLIDSASLH
560 570 580 590 600
LNYGVYLNGT LIGSIRFPTK FVTQFRHLRR TGKVSEFISI YSNSHQMAVH
610 620 630 640 650
IATDGGRICR PLIIVSDGQS RVKDIHLRKL LDGELDFDDF LKLGLVEYLD
660 670 680 690 700
VNEENDSYIA LYEKDIVPSM THLEIEPFTI LGAVAGLIPY PHHNQSPRNT
710 720 730 740 750
YQCAMGKQAI GAIAYNQFKR IDTLLYLMTY PQQPMVKTKT IELIDYDKLP
760 770 780 790 800
AGQNATVAVM SYSGYDIEDA LVLNKSSIDR GFGRCETRRK TTTVLKRYAN
810 820 830 840 850
HTQDIIGGMR VDENGDPIWQ HQSLGPDGLG EVGMKVQSGQ IYINKSVPTN
860 870 880 890 900
SADAPNPNNV NVQTQYREAP VIYRGPEPSH IDQVMMSVSD NDQALIKVLL
910 920 930 940 950
RQNRRPELGD KFSSRHGQKG VCGIIVKQED MPFNDQGIVP DIIMNPHGFP
960 970 980 990 1000
SRMTVGKMIE LISGKAGVLN GTLEYGTCFG GSKLEDMSKI LVDQGFNYSG
1010 1020 1030 1040 1050
KDMLYSGITG ECLQAYIFFG PIYYQKLKHM VLDKMHARAR GPRAVLTRQP
1060 1070 1080 1090 1100
TEGRSRDGGL RLGEMERDCV IAYGASQLLL ERLMISSDAF EVDVCDKCGL
1110 1120 1130 1140
MGYSGWCTTC KSAENIIKMT IPYAAKLLFQ ELLSMNIAPR LRLEDIFQQ
Length:1,149
Mass (Da):129,456
Last modified:November 1, 1997 - v2
Checksum:i6AC52354F34CF090
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti213K → E in AAB59324 (PubMed:2005101).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M38723 Genomic DNA Translation: AAB59324.1
Z75115 Genomic DNA Translation: CAA99422.1
BK006948 Genomic DNA Translation: DAA10979.1
PIRiS67099
RefSeqiNP_014850.1, NM_001183626.1

Genome annotation databases

EnsemblFungiiYOR207C; YOR207C; YOR207C
GeneIDi854382
KEGGisce:YOR207C

Similar proteinsi

Entry informationi

Entry nameiRPC2_YEAST
AccessioniPrimary (citable) accession number: P22276
Secondary accession number(s): D6W2R3, Q12696
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: November 1, 1997
Last modified: May 23, 2018
This is version 161 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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