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Protein

DNA-directed RNA polymerase III subunit RPC2

Gene

RET1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Second largest core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. Pol III is composed of mobile elements and RPC2 is part of the core element with the central large cleft and probably a clamp element that moves to open and close the cleft (By similarity).By similarity

Catalytic activityi

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi1095 – 10951ZincBy similarity
Metal bindingi1098 – 10981ZincBy similarity
Metal bindingi1107 – 11071ZincBy similarity
Metal bindingi1110 – 11101ZincBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri1095 – 111016C4-typeBy similarityAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Biological processi

Transcription

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-33711-MONOMER.
ReactomeiR-SCE-113418. Formation of the Early Elongation Complex.
R-SCE-674695. RNA Polymerase II Pre-transcription Events.
R-SCE-6781823. Formation of TC-NER Pre-Incision Complex.
R-SCE-6782135. Dual incision in TC-NER.
R-SCE-6782210. Gap-filling DNA repair synthesis and ligation in TC-NER.
R-SCE-6796648. TP53 Regulates Transcription of DNA Repair Genes.
R-SCE-72086. mRNA Capping.
R-SCE-72165. mRNA Splicing - Minor Pathway.
R-SCE-73776. RNA Polymerase II Promoter Escape.
R-SCE-73779. RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
R-SCE-73780. RNA Polymerase III Chain Elongation.
R-SCE-73980. RNA Polymerase III Transcription Termination.
R-SCE-749476. RNA Polymerase III Abortive And Retractive Initiation.
R-SCE-75953. RNA Polymerase II Transcription Initiation.
R-SCE-76042. RNA Polymerase II Transcription Initiation And Promoter Clearance.
R-SCE-76061. RNA Polymerase III Transcription Initiation From Type 1 Promoter.
R-SCE-76066. RNA Polymerase III Transcription Initiation From Type 2 Promoter.
R-SCE-76071. RNA Polymerase III Transcription Initiation From Type 3 Promoter.
R-SCE-77075. RNA Pol II CTD phosphorylation and interaction with CE.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-directed RNA polymerase III subunit RPC2 (EC:2.7.7.6)
Short name:
RNA polymerase III subunit C2
Alternative name(s):
C128
DNA-directed RNA polymerase III 130 kDa polypeptide
Gene namesi
Name:RET1
Synonyms:RPC128, RPC2
Ordered Locus Names:YOR207C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOR207C.
SGDiS000005733. RET1.

Subcellular locationi

GO - Cellular componenti

  • DNA-directed RNA polymerase III complex Source: SGD
  • nucleoplasm Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

DNA-directed RNA polymerase, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11491149DNA-directed RNA polymerase III subunit RPC2PRO_0000048096Add
BLAST

Proteomic databases

MaxQBiP22276.

PTM databases

iPTMnetiP22276.

Interactioni

Subunit structurei

Component of the RNA polymerase III (Pol III) complex consisting of 17 subunits.

Protein-protein interaction databases

BioGridi34602. 77 interactions.
DIPiDIP-997N.
IntActiP22276. 26 interactions.
MINTiMINT-644049.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
5FJ8electron microscopy3.90B1-1149[»]
5FJ9electron microscopy4.60B1-1149[»]
5FJAelectron microscopy4.65B1-1149[»]
ProteinModelPortaliP22276.
SMRiP22276. Positions 895-967, 979-1088.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the RNA polymerase beta chain family.Curated

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri1095 – 111016C4-typeBy similarityAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

GeneTreeiENSGT00760000119346.
HOGENOMiHOG000222962.
InParanoidiP22276.
KOiK03021.
OMAiPYIIVKN.
OrthoDBiEOG092C06IY.

Family and domain databases

CDDicd00653. RNA_pol_B_RPB2. 1 hit.
Gene3Di2.40.270.10. 2 hits.
2.40.50.150. 1 hit.
3.90.1110.10. 1 hit.
InterProiIPR015712. DNA-dir_RNA_pol_su2.
IPR007120. DNA-dir_RNA_pol_su2_6.
IPR007121. RNA_pol_bsu_CS.
IPR007644. RNA_pol_bsu_protrusion.
IPR007642. RNA_pol_Rpb2_2.
IPR007645. RNA_pol_Rpb2_3.
IPR007646. RNA_pol_Rpb2_4.
IPR007647. RNA_pol_Rpb2_5.
IPR007641. RNA_pol_Rpb2_7.
IPR014724. RNA_pol_RPB2_OB-fold.
[Graphical view]
PANTHERiPTHR20856. PTHR20856. 1 hit.
PfamiPF04563. RNA_pol_Rpb2_1. 1 hit.
PF04561. RNA_pol_Rpb2_2. 1 hit.
PF04565. RNA_pol_Rpb2_3. 1 hit.
PF04566. RNA_pol_Rpb2_4. 1 hit.
PF04567. RNA_pol_Rpb2_5. 1 hit.
PF00562. RNA_pol_Rpb2_6. 1 hit.
PF04560. RNA_pol_Rpb2_7. 1 hit.
[Graphical view]
PROSITEiPS01166. RNA_POL_BETA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P22276-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVAATKRRKT HIHKHVKDEA FDDLLKPVYK GKKLTDEINT AQDKWHLLPA
60 70 80 90 100
FLKVKGLVKQ HLDSFNYFVD TDLKKIIKAN QLILSDVDPE FYLKYVDIRV
110 120 130 140 150
GKKSSSSTKD YLTPPHECRL RDMTYSAPIY VDIEYTRGRN IIMHKDVEIG
160 170 180 190 200
RMPIMLRSNK CILYDADESK MAKLNECPLD PGGYFIVNGT EKVILVQEQL
210 220 230 240 250
SKNRIIVEAD EKKGIVQASV TSSTHERKSK TYVITKNGKI YLKHNSIAEE
260 270 280 290 300
IPIAIVLKAC GILSDLEIMQ LVCGNDSSYQ DIFAVNLEES SKLDIYTQQQ
310 320 330 340 350
ALEYIGAKVK TMRRQKLTIL QEGIEAIATT VIAHLTVEAL DFREKALYIA
360 370 380 390 400
MMTRRVVMAM YNPKMIDDRD YVGNKRLELA GQLISLLFED LFKKFNNDFK
410 420 430 440 450
LSIDKVLKKP NRAMEYDALL SINVHSNNIT SGLNRAISTG NWSLKRFKME
460 470 480 490 500
RAGVTHVLSR LSYISALGMM TRISSQFEKS RKVSGPRALQ PSQFGMLCTA
510 520 530 540 550
DTPEGEACGL VKNLALMTHI TTDDEEEPIK KLCYVLGVED ITLIDSASLH
560 570 580 590 600
LNYGVYLNGT LIGSIRFPTK FVTQFRHLRR TGKVSEFISI YSNSHQMAVH
610 620 630 640 650
IATDGGRICR PLIIVSDGQS RVKDIHLRKL LDGELDFDDF LKLGLVEYLD
660 670 680 690 700
VNEENDSYIA LYEKDIVPSM THLEIEPFTI LGAVAGLIPY PHHNQSPRNT
710 720 730 740 750
YQCAMGKQAI GAIAYNQFKR IDTLLYLMTY PQQPMVKTKT IELIDYDKLP
760 770 780 790 800
AGQNATVAVM SYSGYDIEDA LVLNKSSIDR GFGRCETRRK TTTVLKRYAN
810 820 830 840 850
HTQDIIGGMR VDENGDPIWQ HQSLGPDGLG EVGMKVQSGQ IYINKSVPTN
860 870 880 890 900
SADAPNPNNV NVQTQYREAP VIYRGPEPSH IDQVMMSVSD NDQALIKVLL
910 920 930 940 950
RQNRRPELGD KFSSRHGQKG VCGIIVKQED MPFNDQGIVP DIIMNPHGFP
960 970 980 990 1000
SRMTVGKMIE LISGKAGVLN GTLEYGTCFG GSKLEDMSKI LVDQGFNYSG
1010 1020 1030 1040 1050
KDMLYSGITG ECLQAYIFFG PIYYQKLKHM VLDKMHARAR GPRAVLTRQP
1060 1070 1080 1090 1100
TEGRSRDGGL RLGEMERDCV IAYGASQLLL ERLMISSDAF EVDVCDKCGL
1110 1120 1130 1140
MGYSGWCTTC KSAENIIKMT IPYAAKLLFQ ELLSMNIAPR LRLEDIFQQ
Length:1,149
Mass (Da):129,456
Last modified:November 1, 1997 - v2
Checksum:i6AC52354F34CF090
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti213 – 2131K → E in AAB59324 (PubMed:2005101).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M38723 Genomic DNA. Translation: AAB59324.1.
Z75115 Genomic DNA. Translation: CAA99422.1.
BK006948 Genomic DNA. Translation: DAA10979.1.
PIRiS67099.
RefSeqiNP_014850.1. NM_001183626.1.

Genome annotation databases

EnsemblFungiiYOR207C; YOR207C; YOR207C.
GeneIDi854382.
KEGGisce:YOR207C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M38723 Genomic DNA. Translation: AAB59324.1.
Z75115 Genomic DNA. Translation: CAA99422.1.
BK006948 Genomic DNA. Translation: DAA10979.1.
PIRiS67099.
RefSeqiNP_014850.1. NM_001183626.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
5FJ8electron microscopy3.90B1-1149[»]
5FJ9electron microscopy4.60B1-1149[»]
5FJAelectron microscopy4.65B1-1149[»]
ProteinModelPortaliP22276.
SMRiP22276. Positions 895-967, 979-1088.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34602. 77 interactions.
DIPiDIP-997N.
IntActiP22276. 26 interactions.
MINTiMINT-644049.

PTM databases

iPTMnetiP22276.

Proteomic databases

MaxQBiP22276.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYOR207C; YOR207C; YOR207C.
GeneIDi854382.
KEGGisce:YOR207C.

Organism-specific databases

EuPathDBiFungiDB:YOR207C.
SGDiS000005733. RET1.

Phylogenomic databases

GeneTreeiENSGT00760000119346.
HOGENOMiHOG000222962.
InParanoidiP22276.
KOiK03021.
OMAiPYIIVKN.
OrthoDBiEOG092C06IY.

Enzyme and pathway databases

BioCyciYEAST:G3O-33711-MONOMER.
ReactomeiR-SCE-113418. Formation of the Early Elongation Complex.
R-SCE-674695. RNA Polymerase II Pre-transcription Events.
R-SCE-6781823. Formation of TC-NER Pre-Incision Complex.
R-SCE-6782135. Dual incision in TC-NER.
R-SCE-6782210. Gap-filling DNA repair synthesis and ligation in TC-NER.
R-SCE-6796648. TP53 Regulates Transcription of DNA Repair Genes.
R-SCE-72086. mRNA Capping.
R-SCE-72165. mRNA Splicing - Minor Pathway.
R-SCE-73776. RNA Polymerase II Promoter Escape.
R-SCE-73779. RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
R-SCE-73780. RNA Polymerase III Chain Elongation.
R-SCE-73980. RNA Polymerase III Transcription Termination.
R-SCE-749476. RNA Polymerase III Abortive And Retractive Initiation.
R-SCE-75953. RNA Polymerase II Transcription Initiation.
R-SCE-76042. RNA Polymerase II Transcription Initiation And Promoter Clearance.
R-SCE-76061. RNA Polymerase III Transcription Initiation From Type 1 Promoter.
R-SCE-76066. RNA Polymerase III Transcription Initiation From Type 2 Promoter.
R-SCE-76071. RNA Polymerase III Transcription Initiation From Type 3 Promoter.
R-SCE-77075. RNA Pol II CTD phosphorylation and interaction with CE.

Miscellaneous databases

PROiP22276.

Family and domain databases

CDDicd00653. RNA_pol_B_RPB2. 1 hit.
Gene3Di2.40.270.10. 2 hits.
2.40.50.150. 1 hit.
3.90.1110.10. 1 hit.
InterProiIPR015712. DNA-dir_RNA_pol_su2.
IPR007120. DNA-dir_RNA_pol_su2_6.
IPR007121. RNA_pol_bsu_CS.
IPR007644. RNA_pol_bsu_protrusion.
IPR007642. RNA_pol_Rpb2_2.
IPR007645. RNA_pol_Rpb2_3.
IPR007646. RNA_pol_Rpb2_4.
IPR007647. RNA_pol_Rpb2_5.
IPR007641. RNA_pol_Rpb2_7.
IPR014724. RNA_pol_RPB2_OB-fold.
[Graphical view]
PANTHERiPTHR20856. PTHR20856. 1 hit.
PfamiPF04563. RNA_pol_Rpb2_1. 1 hit.
PF04561. RNA_pol_Rpb2_2. 1 hit.
PF04565. RNA_pol_Rpb2_3. 1 hit.
PF04566. RNA_pol_Rpb2_4. 1 hit.
PF04567. RNA_pol_Rpb2_5. 1 hit.
PF00562. RNA_pol_Rpb2_6. 1 hit.
PF04560. RNA_pol_Rpb2_7. 1 hit.
[Graphical view]
PROSITEiPS01166. RNA_POL_BETA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRPC2_YEAST
AccessioniPrimary (citable) accession number: P22276
Secondary accession number(s): D6W2R3, Q12696
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: November 1, 1997
Last modified: September 7, 2016
This is version 145 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1950 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.