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Protein

DNA-directed RNA polymerase III subunit RPC2

Gene

RET1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Second largest core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. Pol III is composed of mobile elements and RPC2 is part of the core element with the central large cleft and probably a clamp element that moves to open and close the cleft (By similarity).By similarity

Miscellaneous

Present with 1950 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi1095ZincBy similarity1
Metal bindingi1098ZincBy similarity1
Metal bindingi1107ZincBy similarity1
Metal bindingi1110ZincBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri1095 – 1110C4-typeBy similarityAdd BLAST16

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionNucleotidyltransferase, Transferase
Biological processTranscription
LigandMetal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-33711-MONOMER.
ReactomeiR-SCE-73780. RNA Polymerase III Chain Elongation.
R-SCE-73980. RNA Polymerase III Transcription Termination.
R-SCE-749476. RNA Polymerase III Abortive And Retractive Initiation.
R-SCE-76061. RNA Polymerase III Transcription Initiation From Type 1 Promoter.
R-SCE-76066. RNA Polymerase III Transcription Initiation From Type 2 Promoter.
R-SCE-76071. RNA Polymerase III Transcription Initiation From Type 3 Promoter.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-directed RNA polymerase III subunit RPC2 (EC:2.7.7.6)
Short name:
RNA polymerase III subunit C2
Alternative name(s):
C128
DNA-directed RNA polymerase III 130 kDa polypeptide
Gene namesi
Name:RET1
Synonyms:RPC128, RPC2
Ordered Locus Names:YOR207C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOR207C.
SGDiS000005733. RET1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

DNA-directed RNA polymerase, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000480961 – 1149DNA-directed RNA polymerase III subunit RPC2Add BLAST1149

Proteomic databases

MaxQBiP22276.
PRIDEiP22276.

PTM databases

iPTMnetiP22276.

Interactioni

Subunit structurei

Component of the RNA polymerase III (Pol III) complex consisting of 17 subunits.

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi34602. 79 interactors.
DIPiDIP-997N.
IntActiP22276. 42 interactors.
MINTiMINT-644049.
STRINGi4932.YOR207C.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5FJ8electron microscopy3.90B1-1149[»]
5FJ9electron microscopy4.60B1-1149[»]
5FJAelectron microscopy4.65B1-1149[»]
ProteinModelPortaliP22276.
SMRiP22276.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the RNA polymerase beta chain family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri1095 – 1110C4-typeBy similarityAdd BLAST16

Keywords - Domaini

Zinc-finger

Phylogenomic databases

GeneTreeiENSGT00880000138161.
HOGENOMiHOG000222962.
InParanoidiP22276.
KOiK03021.
OMAiMLCTSDT.
OrthoDBiEOG092C06IY.

Family and domain databases

CDDicd00653. RNA_pol_B_RPB2. 1 hit.
Gene3Di2.40.270.10. 2 hits.
3.90.1110.10. 1 hit.
InterProiView protein in InterPro
IPR015712. DNA-dir_RNA_pol_su2.
IPR007120. DNA-dir_RNA_pol_su2_6.
IPR007121. RNA_pol_bsu_CS.
IPR007644. RNA_pol_bsu_protrusion.
IPR007642. RNA_pol_Rpb2_2.
IPR007645. RNA_pol_Rpb2_3.
IPR007646. RNA_pol_Rpb2_4.
IPR007647. RNA_pol_Rpb2_5.
IPR007641. RNA_pol_Rpb2_7.
PANTHERiPTHR20856. PTHR20856. 1 hit.
PfamiView protein in Pfam
PF04563. RNA_pol_Rpb2_1. 1 hit.
PF04561. RNA_pol_Rpb2_2. 1 hit.
PF04565. RNA_pol_Rpb2_3. 1 hit.
PF04566. RNA_pol_Rpb2_4. 1 hit.
PF04567. RNA_pol_Rpb2_5. 1 hit.
PF00562. RNA_pol_Rpb2_6. 1 hit.
PF04560. RNA_pol_Rpb2_7. 1 hit.
PROSITEiView protein in PROSITE
PS01166. RNA_POL_BETA. 1 hit.

Sequencei

Sequence statusi: Complete.

P22276-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVAATKRRKT HIHKHVKDEA FDDLLKPVYK GKKLTDEINT AQDKWHLLPA
60 70 80 90 100
FLKVKGLVKQ HLDSFNYFVD TDLKKIIKAN QLILSDVDPE FYLKYVDIRV
110 120 130 140 150
GKKSSSSTKD YLTPPHECRL RDMTYSAPIY VDIEYTRGRN IIMHKDVEIG
160 170 180 190 200
RMPIMLRSNK CILYDADESK MAKLNECPLD PGGYFIVNGT EKVILVQEQL
210 220 230 240 250
SKNRIIVEAD EKKGIVQASV TSSTHERKSK TYVITKNGKI YLKHNSIAEE
260 270 280 290 300
IPIAIVLKAC GILSDLEIMQ LVCGNDSSYQ DIFAVNLEES SKLDIYTQQQ
310 320 330 340 350
ALEYIGAKVK TMRRQKLTIL QEGIEAIATT VIAHLTVEAL DFREKALYIA
360 370 380 390 400
MMTRRVVMAM YNPKMIDDRD YVGNKRLELA GQLISLLFED LFKKFNNDFK
410 420 430 440 450
LSIDKVLKKP NRAMEYDALL SINVHSNNIT SGLNRAISTG NWSLKRFKME
460 470 480 490 500
RAGVTHVLSR LSYISALGMM TRISSQFEKS RKVSGPRALQ PSQFGMLCTA
510 520 530 540 550
DTPEGEACGL VKNLALMTHI TTDDEEEPIK KLCYVLGVED ITLIDSASLH
560 570 580 590 600
LNYGVYLNGT LIGSIRFPTK FVTQFRHLRR TGKVSEFISI YSNSHQMAVH
610 620 630 640 650
IATDGGRICR PLIIVSDGQS RVKDIHLRKL LDGELDFDDF LKLGLVEYLD
660 670 680 690 700
VNEENDSYIA LYEKDIVPSM THLEIEPFTI LGAVAGLIPY PHHNQSPRNT
710 720 730 740 750
YQCAMGKQAI GAIAYNQFKR IDTLLYLMTY PQQPMVKTKT IELIDYDKLP
760 770 780 790 800
AGQNATVAVM SYSGYDIEDA LVLNKSSIDR GFGRCETRRK TTTVLKRYAN
810 820 830 840 850
HTQDIIGGMR VDENGDPIWQ HQSLGPDGLG EVGMKVQSGQ IYINKSVPTN
860 870 880 890 900
SADAPNPNNV NVQTQYREAP VIYRGPEPSH IDQVMMSVSD NDQALIKVLL
910 920 930 940 950
RQNRRPELGD KFSSRHGQKG VCGIIVKQED MPFNDQGIVP DIIMNPHGFP
960 970 980 990 1000
SRMTVGKMIE LISGKAGVLN GTLEYGTCFG GSKLEDMSKI LVDQGFNYSG
1010 1020 1030 1040 1050
KDMLYSGITG ECLQAYIFFG PIYYQKLKHM VLDKMHARAR GPRAVLTRQP
1060 1070 1080 1090 1100
TEGRSRDGGL RLGEMERDCV IAYGASQLLL ERLMISSDAF EVDVCDKCGL
1110 1120 1130 1140
MGYSGWCTTC KSAENIIKMT IPYAAKLLFQ ELLSMNIAPR LRLEDIFQQ
Length:1,149
Mass (Da):129,456
Last modified:November 1, 1997 - v2
Checksum:i6AC52354F34CF090
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti213K → E in AAB59324 (PubMed:2005101).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M38723 Genomic DNA. Translation: AAB59324.1.
Z75115 Genomic DNA. Translation: CAA99422.1.
BK006948 Genomic DNA. Translation: DAA10979.1.
PIRiS67099.
RefSeqiNP_014850.1. NM_001183626.1.

Genome annotation databases

EnsemblFungiiYOR207C; YOR207C; YOR207C.
GeneIDi854382.
KEGGisce:YOR207C.

Similar proteinsi

Entry informationi

Entry nameiRPC2_YEAST
AccessioniPrimary (citable) accession number: P22276
Secondary accession number(s): D6W2R3, Q12696
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: November 1, 1997
Last modified: September 27, 2017
This is version 154 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names