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P22266

- ESTA_STRSC

UniProt

P22266 - ESTA_STRSC

Protein

Esterase

Gene

estA

Organism
Streptomyces scabiei
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 68 (01 Oct 2014)
      Sequence version 1 (01 Aug 1991)
      Previous versions | rss
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    Functioni

    GO - Molecular functioni

    1. hydrolase activity Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase, Serine esterase

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Esterase (EC:3.1.1.-)
    Gene namesi
    Name:estA
    OrganismiStreptomyces scabiei
    Taxonomic identifieri1930 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomyces

    Subcellular locationi

    Secreted 1 Publication

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 39391 PublicationAdd
    BLAST
    Chaini40 – 345306EsterasePRO_0000021208Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi73 ↔ 103
    Disulfide bondi156 ↔ 180
    Disulfide bondi236 ↔ 294

    Keywords - PTMi

    Disulfide bond

    Expressioni

    Inductioni

    By zinc.

    Structurei

    Secondary structure

    1
    345
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi44 – 496
    Helixi53 – 564
    Turni57 – 593
    Beta strandi61 – 633
    Turni64 – 674
    Helixi69 – 735
    Helixi80 – 8910
    Turni90 – 923
    Beta strandi94 – 1007
    Helixi108 – 1114
    Beta strandi115 – 1173
    Helixi118 – 1203
    Beta strandi122 – 1243
    Helixi127 – 1304
    Beta strandi137 – 1404
    Helixi144 – 1474
    Helixi149 – 1557
    Turni158 – 1614
    Beta strandi171 – 1755
    Helixi177 – 1793
    Helixi180 – 1834
    Turni184 – 1863
    Helixi188 – 21427
    Beta strandi219 – 2235
    Helixi233 – 2375
    Turni247 – 2504
    Turni253 – 2553
    Helixi256 – 27520
    Turni276 – 2783
    Beta strandi280 – 2823
    Helixi285 – 2873
    Beta strandi304 – 31411
    Beta strandi316 – 3183
    Helixi325 – 34218

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1ESCX-ray2.10A40-345[»]
    1ESDX-ray2.30A40-345[»]
    1ESEX-ray2.40A40-345[»]
    ProteinModelPortaliP22266.
    SMRiP22266. Positions 43-344.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP22266.

    Family & Domainsi

    Keywords - Domaini

    Signal

    Family and domain databases

    Gene3Di3.40.50.1110. 1 hit.
    InterProiIPR013831. SGNH_hydro-type_esterase_dom.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P22266-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSSAMRKTTN SPVVRRLTAA AVALGSCLAL AGPAGSAGAA PADPVPTVFF    50
    GDSYTANFGI APVTNQDSER GWCFQAKENY PAVATRSLAD KGITLDVQAD 100
    VSCGGALIHH FWEKQELPFG AGELPPQQDA LKQDTQLTVG SLGGNTLGFN 150
    RILKQCSDEL RKPSLLPGDP VDGDEPAAKC GEFFGTGDGK QWLDDQFERV 200
    GAELEELLDR IGYFAPDAKR VLVGYPRLVP EDTTKCLTAA PGQTQLPFAD 250
    IPQDALPVLD QIQKRLNDAM KKAAADGGAD FVDLYAGTGA NTACDGADRG 300
    IGGLLEDSQL ELLGTKIPWY AHPNDKGRDI QAKQVADKIE EILNR 345
    Length:345
    Mass (Da):36,670
    Last modified:August 1, 1991 - v1
    Checksum:i237DC28EE185B00E
    GO

    Sequence cautioni

    The sequence AAA26744.1 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M57297 Genomic DNA. Translation: AAA26743.1.
    M57297 Genomic DNA. Translation: AAA26744.1. Different initiation.
    PIRiA37845.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M57297 Genomic DNA. Translation: AAA26743.1 .
    M57297 Genomic DNA. Translation: AAA26744.1 . Different initiation.
    PIRi A37845.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1ESC X-ray 2.10 A 40-345 [» ]
    1ESD X-ray 2.30 A 40-345 [» ]
    1ESE X-ray 2.40 A 40-345 [» ]
    ProteinModelPortali P22266.
    SMRi P22266. Positions 43-344.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Miscellaneous databases

    EvolutionaryTracei P22266.

    Family and domain databases

    Gene3Di 3.40.50.1110. 1 hit.
    InterProi IPR013831. SGNH_hydro-type_esterase_dom.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning, sequencing, and regulation of expression of an extracellular esterase gene from the plant pathogen Streptomyces scabies."
      Raymer G., Willard J.M.A., Schottel J.L.
      J. Bacteriol. 172:7020-7026(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 40-69.
      Strain: FL1.
    2. "Regulation and secretion of an extracellular esterase from Streptomyces scabies."
      Schottel J.L., Hale V., Babcock M.J.
      Gene 115:27-31(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
    3. "A novel variant of the catalytic triad in the Streptomyces scabies esterase."
      Wei Y., Schottel J.L., Derewenda U., Swenson L., Patkar S., Derewenda Z.S.
      Nat. Struct. Biol. 2:218-223(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS).

    Entry informationi

    Entry nameiESTA_STRSC
    AccessioniPrimary (citable) accession number: P22266
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1991
    Last sequence update: August 1, 1991
    Last modified: October 1, 2014
    This is version 68 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Caution

    It is uncertain whether Met-1 or Met-5 is the initiator.Curated

    Keywords - Technical termi

    3D-structure, Direct protein sequencing

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references

    External Data

    Dasty 3