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P22266 (ESTA_STRSC) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 67. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Esterase

EC=3.1.1.-
Gene names
Name:estA
OrganismStreptomyces scabiei
Taxonomic identifier1930 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomyces

Protein attributes

Sequence length345 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Subcellular location

Secreted Ref.2.

Induction

By zinc.

Caution

It is uncertain whether Met-1 or Met-5 is the initiator.

Sequence caution

The sequence AAA26744.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Cellular componentSecreted
   DomainSignal
   Molecular functionHydrolase
Serine esterase
   PTMDisulfide bond
   Technical term3D-structure
Direct protein sequencing
Gene Ontology (GO)
   Cellular_componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionhydrolase activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3939 Ref.1
Chain40 – 345306Esterase
PRO_0000021208

Amino acid modifications

Disulfide bond73 ↔ 103
Disulfide bond156 ↔ 180
Disulfide bond236 ↔ 294

Secondary structure

............................................................. 345
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P22266 [UniParc].

Last modified August 1, 1991. Version 1.
Checksum: 237DC28EE185B00E

FASTA34536,670
        10         20         30         40         50         60 
MSSAMRKTTN SPVVRRLTAA AVALGSCLAL AGPAGSAGAA PADPVPTVFF GDSYTANFGI 

        70         80         90        100        110        120 
APVTNQDSER GWCFQAKENY PAVATRSLAD KGITLDVQAD VSCGGALIHH FWEKQELPFG 

       130        140        150        160        170        180 
AGELPPQQDA LKQDTQLTVG SLGGNTLGFN RILKQCSDEL RKPSLLPGDP VDGDEPAAKC 

       190        200        210        220        230        240 
GEFFGTGDGK QWLDDQFERV GAELEELLDR IGYFAPDAKR VLVGYPRLVP EDTTKCLTAA 

       250        260        270        280        290        300 
PGQTQLPFAD IPQDALPVLD QIQKRLNDAM KKAAADGGAD FVDLYAGTGA NTACDGADRG 

       310        320        330        340 
IGGLLEDSQL ELLGTKIPWY AHPNDKGRDI QAKQVADKIE EILNR 

« Hide

References

[1]"Cloning, sequencing, and regulation of expression of an extracellular esterase gene from the plant pathogen Streptomyces scabies."
Raymer G., Willard J.M.A., Schottel J.L.
J. Bacteriol. 172:7020-7026(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 40-69.
Strain: FL1.
[2]"Regulation and secretion of an extracellular esterase from Streptomyces scabies."
Schottel J.L., Hale V., Babcock M.J.
Gene 115:27-31(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[3]"A novel variant of the catalytic triad in the Streptomyces scabies esterase."
Wei Y., Schottel J.L., Derewenda U., Swenson L., Patkar S., Derewenda Z.S.
Nat. Struct. Biol. 2:218-223(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS).

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M57297 Genomic DNA. Translation: AAA26743.1.
M57297 Genomic DNA. Translation: AAA26744.1. Different initiation.
PIRA37845.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1ESCX-ray2.10A40-345[»]
1ESDX-ray2.30A40-345[»]
1ESEX-ray2.40A40-345[»]
ProteinModelPortalP22266.
SMRP22266. Positions 43-344.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.40.50.1110. 1 hit.
InterProIPR013831. SGNH_hydro-type_esterase_dom.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP22266.

Entry information

Entry nameESTA_STRSC
AccessionPrimary (citable) accession number: P22266
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: August 1, 1991
Last modified: April 16, 2014
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references