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P22262

- PRFA_LISMO

UniProt

P22262 - PRFA_LISMO

Protein

Listeriolysin regulatory protein

Gene

prfA

Organism
Listeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e)
Status
Reviewed - Annotation score: 2 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 110 (01 Oct 2014)
      Sequence version 2 (01 Feb 1994)
      Previous versions | rss
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    Functioni

    Positively regulates expression of listeriolysin, of 1-phosphadidylinositol phosphodiesterase (PI-PLC) and other virulence factors.

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-KW
    2. sequence-specific DNA binding transcription factor activity Source: InterPro

    GO - Biological processi

    1. pathogenesis Source: UniProtKB-KW
    2. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Activator

    Keywords - Biological processi

    Transcription, Transcription regulation, Virulence

    Keywords - Ligandi

    DNA-binding

    Enzyme and pathway databases

    BioCyciLMON169963:LMO0200-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Listeriolysin regulatory protein
    Gene namesi
    Name:prfA
    Ordered Locus Names:lmo0200
    OrganismiListeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e)
    Taxonomic identifieri169963 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesListeriaceaeListeria
    ProteomesiUP000000817: Chromosome

    Organism-specific databases

    GenoListiLMO0200.

    Subcellular locationi

    GO - Cellular componenti

    1. intracellular Source: InterPro

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 237237Listeriolysin regulatory proteinPRO_0000100188Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi169963.lmo0200.

    Structurei

    Secondary structure

    1
    237
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi4 – 1411
    Beta strandi20 – 234
    Beta strandi27 – 293
    Beta strandi37 – 5115
    Beta strandi57 – 7216
    Turni74 – 763
    Beta strandi84 – 874
    Beta strandi89 – 9810
    Helixi99 – 10810
    Helixi110 – 13526
    Helixi138 – 15417
    Beta strandi155 – 1584
    Beta strandi161 – 1644
    Turni168 – 1703
    Helixi171 – 1777
    Helixi183 – 19513
    Beta strandi198 – 2025
    Beta strandi205 – 2095
    Helixi211 – 2177
    Helixi219 – 22810
    Helixi230 – 2345

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1OMIX-ray2.80A/B7-237[»]
    2BEOX-ray2.70A/B2-237[»]
    2BGCX-ray2.30A/B/D/E/F/G/H/I2-237[»]
    ProteinModelPortaliP22262.
    SMRiP22262. Positions 2-237.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP22262.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini137 – 21276HTH crp-typePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 HTH crp-type DNA-binding domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0664.
    OMAiAIAREDC.
    OrthoDBiEOG6R87DH.
    PhylomeDBiP22262.

    Family and domain databases

    Gene3Di1.10.10.10. 1 hit.
    2.60.120.10. 1 hit.
    InterProiIPR018490. cNMP-bd-like.
    IPR012318. HTH_CRP_2.
    IPR014710. RmlC-like_jellyroll.
    IPR018335. Tscrpt_reg_HTH_Crp-type_CS.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view]
    SUPFAMiSSF51206. SSF51206. 1 hit.
    PROSITEiPS00042. HTH_CRP_1. 1 hit.
    PS51063. HTH_CRP_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P22262-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNAQAEEFKK YLETNGIKPK QFHKKELIFN QWDPQEYCIF LYDGITKLTS    50
    ISENGTIMNL QYYKGAFVIM SGFIDTETSV GYYNLEVISE QATAYVIKIN 100
    ELKELLSKNL THFFYVFQTL QKQVSYSLAK FNDFSINGKL GSICGQLLIL 150
    TYVYGKETPD GIKITLDNLT MQELGYSSGI AHSSAVSRII SKLKQEKVIV 200
    YKNSCFYVQN LDYLKRYAPK LDEWFYLACP ATWGKLN 237
    Length:237
    Mass (Da):27,301
    Last modified:February 1, 1994 - v2
    Checksum:i697542B135158CFB
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti234 – 2363GKL → E(PubMed:2122460)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti145 – 1451G → S in prfA* mutant which constitutively overexpresses virulence genes. Presumably blocks prfA in a cofactor-independent transcriptionally active conformation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X61210 Genomic DNA. Translation: CAA43524.1.
    M55160 Genomic DNA. Translation: AAA25291.1.
    AJ002742 Genomic DNA. Translation: CAA05714.1.
    AL591974 Genomic DNA. Translation: CAD00727.1.
    PIRiAI1099.
    S16890.
    RefSeqiNP_463731.1. NC_003210.1.
    WP_003722729.1. NC_003210.1.
    YP_007602416.1. NC_020557.1.
    YP_008273512.1. NC_021823.1.
    YP_008274522.1. NC_021824.1.
    YP_008279488.1. NC_021825.1.
    YP_008281766.1. NC_021826.1.
    YP_008282691.1. NC_021827.1.
    YP_008298400.1. NC_021838.1.
    YP_008303431.1. NC_021839.1.
    YP_008305045.1. NC_021840.1.

    Genome annotation databases

    EnsemblBacteriaiCAD00727; CAD00727; CAD00727.
    GeneIDi14824603.
    16308916.
    16309966.
    16315092.
    16320614.
    16324568.
    16335585.
    16340760.
    16342549.
    987031.
    KEGGilmo:lmo0200.
    lmoe:BN418_0210.
    PATRICi20309407. VBILisMon69206_0205.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X61210 Genomic DNA. Translation: CAA43524.1 .
    M55160 Genomic DNA. Translation: AAA25291.1 .
    AJ002742 Genomic DNA. Translation: CAA05714.1 .
    AL591974 Genomic DNA. Translation: CAD00727.1 .
    PIRi AI1099.
    S16890.
    RefSeqi NP_463731.1. NC_003210.1.
    WP_003722729.1. NC_003210.1.
    YP_007602416.1. NC_020557.1.
    YP_008273512.1. NC_021823.1.
    YP_008274522.1. NC_021824.1.
    YP_008279488.1. NC_021825.1.
    YP_008281766.1. NC_021826.1.
    YP_008282691.1. NC_021827.1.
    YP_008298400.1. NC_021838.1.
    YP_008303431.1. NC_021839.1.
    YP_008305045.1. NC_021840.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1OMI X-ray 2.80 A/B 7-237 [» ]
    2BEO X-ray 2.70 A/B 2-237 [» ]
    2BGC X-ray 2.30 A/B/D/E/F/G/H/I 2-237 [» ]
    ProteinModelPortali P22262.
    SMRi P22262. Positions 2-237.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 169963.lmo0200.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAD00727 ; CAD00727 ; CAD00727 .
    GeneIDi 14824603.
    16308916.
    16309966.
    16315092.
    16320614.
    16324568.
    16335585.
    16340760.
    16342549.
    987031.
    KEGGi lmo:lmo0200.
    lmoe:BN418_0210.
    PATRICi 20309407. VBILisMon69206_0205.

    Organism-specific databases

    GenoListi LMO0200.

    Phylogenomic databases

    eggNOGi COG0664.
    OMAi AIAREDC.
    OrthoDBi EOG6R87DH.
    PhylomeDBi P22262.

    Enzyme and pathway databases

    BioCyci LMON169963:LMO0200-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P22262.

    Family and domain databases

    Gene3Di 1.10.10.10. 1 hit.
    2.60.120.10. 1 hit.
    InterProi IPR018490. cNMP-bd-like.
    IPR012318. HTH_CRP_2.
    IPR014710. RmlC-like_jellyroll.
    IPR018335. Tscrpt_reg_HTH_Crp-type_CS.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view ]
    SUPFAMi SSF51206. SSF51206. 1 hit.
    PROSITEi PS00042. HTH_CRP_1. 1 hit.
    PS51063. HTH_CRP_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Pleiotropic control of Listeria monocytogenes virulence factors by a gene that is autoregulated."
      Mengaud J., Dramsi S., Gouin E., Vazquez-Boland J.A., Milon G., Cossart P.
      Mol. Microbiol. 5:2273-2283(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: LO28 / Serovar 1/2c.
    2. "Identification of a gene that positively regulates expression of listeriolysin, the major virulence factor of listeria monocytogenes."
      Leimeister-Waechter M., Haffner C., Domann E., Goebel W., Chakraborty T.
      Proc. Natl. Acad. Sci. U.S.A. 87:8336-8340(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: EGD / Serovar 1/2a.
    3. "A Gly145Ser substitution in the transcriptional activator PrfA causes constitutive overexpression of virulence factors in Listeria monocytogenes."
      Ripio M.T., Dominguez-Bernal G., Lara M., Suarez M., Vazquez-Boland J.A.
      J. Bacteriol. 179:1533-1540(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: P14 / Serovar 4B.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC BAA-679 / EGD-e.

    Entry informationi

    Entry nameiPRFA_LISMO
    AccessioniPrimary (citable) accession number: P22262
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1991
    Last sequence update: February 1, 1994
    Last modified: October 1, 2014
    This is version 110 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3