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Protein

Listeriolysin regulatory protein

Gene

prfA

Organism
Listeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Positively regulates expression of listeriolysin, of 1-phosphadidylinositol phosphodiesterase (PI-PLC) and other virulence factors.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation, Virulence

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Listeriolysin regulatory protein
Gene namesi
Name:prfA
Ordered Locus Names:lmo0200
OrganismiListeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e)
Taxonomic identifieri169963 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesListeriaceaeListeria
Proteomesi
  • UP000000817 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001001881 – 237Listeriolysin regulatory proteinAdd BLAST237

Proteomic databases

PaxDbiP22262.

Interactioni

Protein-protein interaction databases

STRINGi169963.lmo0200.

Structurei

Secondary structure

1237
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi3 – 14Combined sources12
Beta strandi19 – 23Combined sources5
Beta strandi27 – 29Combined sources3
Beta strandi37 – 51Combined sources15
Beta strandi57 – 72Combined sources16
Turni74 – 76Combined sources3
Beta strandi79 – 81Combined sources3
Beta strandi84 – 87Combined sources4
Beta strandi89 – 98Combined sources10
Helixi99 – 107Combined sources9
Helixi110 – 154Combined sources45
Beta strandi155 – 158Combined sources4
Beta strandi161 – 164Combined sources4
Helixi168 – 174Combined sources7
Helixi184 – 195Combined sources12
Beta strandi198 – 202Combined sources5
Beta strandi205 – 208Combined sources4
Helixi211 – 217Combined sources7
Helixi219 – 228Combined sources10
Helixi230 – 234Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1OMIX-ray2.80A/B7-237[»]
2BEOX-ray2.70A/B2-237[»]
2BGCX-ray2.30A/B/D/E/F/G/H/I2-237[»]
5F1RX-ray2.25A/B1-237[»]
ProteinModelPortaliP22262.
SMRiP22262.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP22262.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini137 – 212HTH crp-typePROSITE-ProRule annotationAdd BLAST76

Sequence similaritiesi

Contains 1 HTH crp-type DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0664. LUCA.
OMAiKGAFIIM.
PhylomeDBiP22262.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
2.60.120.10. 1 hit.
InterProiIPR018490. cNMP-bd-like.
IPR012318. HTH_CRP.
IPR014710. RmlC-like_jellyroll.
IPR018335. Tscrpt_reg_HTH_Crp-type_CS.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF13545. HTH_Crp_2. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF51206. SSF51206. 1 hit.
PROSITEiPS00042. HTH_CRP_1. 1 hit.
PS51063. HTH_CRP_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P22262-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNAQAEEFKK YLETNGIKPK QFHKKELIFN QWDPQEYCIF LYDGITKLTS
60 70 80 90 100
ISENGTIMNL QYYKGAFVIM SGFIDTETSV GYYNLEVISE QATAYVIKIN
110 120 130 140 150
ELKELLSKNL THFFYVFQTL QKQVSYSLAK FNDFSINGKL GSICGQLLIL
160 170 180 190 200
TYVYGKETPD GIKITLDNLT MQELGYSSGI AHSSAVSRII SKLKQEKVIV
210 220 230
YKNSCFYVQN LDYLKRYAPK LDEWFYLACP ATWGKLN
Length:237
Mass (Da):27,301
Last modified:February 1, 1994 - v2
Checksum:i697542B135158CFB
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti234 – 236GKL → E (PubMed:2122460).Curated3

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti145G → S in prfA* mutant which constitutively overexpresses virulence genes. Presumably blocks prfA in a cofactor-independent transcriptionally active conformation. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X61210 Genomic DNA. Translation: CAA43524.1.
M55160 Genomic DNA. Translation: AAA25291.1.
AJ002742 Genomic DNA. Translation: CAA05714.1.
AL591974 Genomic DNA. Translation: CAD00727.1.
PIRiAI1099.
S16890.
RefSeqiNP_463731.1. NC_003210.1.
WP_003722729.1. NC_003210.1.

Genome annotation databases

EnsemblBacteriaiCAD00727; CAD00727; CAD00727.
GeneIDi987031.
KEGGilmo:lmo0200.
PATRICi20309407. VBILisMon69206_0205.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X61210 Genomic DNA. Translation: CAA43524.1.
M55160 Genomic DNA. Translation: AAA25291.1.
AJ002742 Genomic DNA. Translation: CAA05714.1.
AL591974 Genomic DNA. Translation: CAD00727.1.
PIRiAI1099.
S16890.
RefSeqiNP_463731.1. NC_003210.1.
WP_003722729.1. NC_003210.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1OMIX-ray2.80A/B7-237[»]
2BEOX-ray2.70A/B2-237[»]
2BGCX-ray2.30A/B/D/E/F/G/H/I2-237[»]
5F1RX-ray2.25A/B1-237[»]
ProteinModelPortaliP22262.
SMRiP22262.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi169963.lmo0200.

Proteomic databases

PaxDbiP22262.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAD00727; CAD00727; CAD00727.
GeneIDi987031.
KEGGilmo:lmo0200.
PATRICi20309407. VBILisMon69206_0205.

Phylogenomic databases

eggNOGiCOG0664. LUCA.
OMAiKGAFIIM.
PhylomeDBiP22262.

Miscellaneous databases

EvolutionaryTraceiP22262.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
2.60.120.10. 1 hit.
InterProiIPR018490. cNMP-bd-like.
IPR012318. HTH_CRP.
IPR014710. RmlC-like_jellyroll.
IPR018335. Tscrpt_reg_HTH_Crp-type_CS.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF13545. HTH_Crp_2. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF51206. SSF51206. 1 hit.
PROSITEiPS00042. HTH_CRP_1. 1 hit.
PS51063. HTH_CRP_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPRFA_LISMO
AccessioniPrimary (citable) accession number: P22262
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: February 1, 1994
Last modified: November 2, 2016
This is version 126 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.