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P22262

- PRFA_LISMO

UniProt

P22262 - PRFA_LISMO

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Protein

Listeriolysin regulatory protein

Gene

prfA

Organism
Listeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e)
Status
Reviewed - Annotation score: 2 out of 5- Experimental evidence at protein leveli

Functioni

Positively regulates expression of listeriolysin, of 1-phosphadidylinositol phosphodiesterase (PI-PLC) and other virulence factors.

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-KW
  2. sequence-specific DNA binding transcription factor activity Source: InterPro

GO - Biological processi

  1. pathogenesis Source: UniProtKB-KW
  2. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation, Virulence

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciLMON169963:LMO0200-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Listeriolysin regulatory protein
Gene namesi
Name:prfA
Ordered Locus Names:lmo0200
OrganismiListeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e)
Taxonomic identifieri169963 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesListeriaceaeListeria
ProteomesiUP000000817: Chromosome

Organism-specific databases

GenoListiLMO0200.

Subcellular locationi

GO - Cellular componenti

  1. intracellular Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 237237Listeriolysin regulatory proteinPRO_0000100188Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi169963.lmo0200.

Structurei

Secondary structure

1
237
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi4 – 1411
Beta strandi20 – 234
Beta strandi27 – 293
Beta strandi37 – 5115
Beta strandi57 – 7216
Turni74 – 763
Beta strandi84 – 874
Beta strandi89 – 9810
Helixi99 – 10810
Helixi110 – 13526
Helixi138 – 15417
Beta strandi155 – 1584
Beta strandi161 – 1644
Turni168 – 1703
Helixi171 – 1777
Helixi183 – 19513
Beta strandi198 – 2025
Beta strandi205 – 2095
Helixi211 – 2177
Helixi219 – 22810
Helixi230 – 2345

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1OMIX-ray2.80A/B7-237[»]
2BEOX-ray2.70A/B2-237[»]
2BGCX-ray2.30A/B/D/E/F/G/H/I2-237[»]
ProteinModelPortaliP22262.
SMRiP22262. Positions 2-237.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP22262.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini137 – 21276HTH crp-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 HTH crp-type DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0664.
OMAiAIAREDC.
OrthoDBiEOG6R87DH.
PhylomeDBiP22262.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
2.60.120.10. 1 hit.
InterProiIPR018490. cNMP-bd-like.
IPR012318. HTH_CRP_2.
IPR014710. RmlC-like_jellyroll.
IPR018335. Tscrpt_reg_HTH_Crp-type_CS.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
SUPFAMiSSF51206. SSF51206. 1 hit.
PROSITEiPS00042. HTH_CRP_1. 1 hit.
PS51063. HTH_CRP_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P22262-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNAQAEEFKK YLETNGIKPK QFHKKELIFN QWDPQEYCIF LYDGITKLTS
60 70 80 90 100
ISENGTIMNL QYYKGAFVIM SGFIDTETSV GYYNLEVISE QATAYVIKIN
110 120 130 140 150
ELKELLSKNL THFFYVFQTL QKQVSYSLAK FNDFSINGKL GSICGQLLIL
160 170 180 190 200
TYVYGKETPD GIKITLDNLT MQELGYSSGI AHSSAVSRII SKLKQEKVIV
210 220 230
YKNSCFYVQN LDYLKRYAPK LDEWFYLACP ATWGKLN
Length:237
Mass (Da):27,301
Last modified:February 1, 1994 - v2
Checksum:i697542B135158CFB
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti234 – 2363GKL → E(PubMed:2122460)Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti145 – 1451G → S in prfA* mutant which constitutively overexpresses virulence genes. Presumably blocks prfA in a cofactor-independent transcriptionally active conformation.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X61210 Genomic DNA. Translation: CAA43524.1.
M55160 Genomic DNA. Translation: AAA25291.1.
AJ002742 Genomic DNA. Translation: CAA05714.1.
AL591974 Genomic DNA. Translation: CAD00727.1.
PIRiAI1099.
S16890.
RefSeqiNP_463731.1. NC_003210.1.
WP_003722729.1. NC_003210.1.
YP_007602416.1. NC_020557.1.
YP_008273512.1. NC_021823.1.
YP_008274522.1. NC_021824.1.
YP_008279488.1. NC_021825.1.
YP_008281766.1. NC_021826.1.
YP_008282691.1. NC_021827.1.
YP_008298400.1. NC_021838.1.
YP_008303431.1. NC_021839.1.
YP_008305045.1. NC_021840.1.

Genome annotation databases

EnsemblBacteriaiCAD00727; CAD00727; CAD00727.
GeneIDi14824603.
16308916.
16309966.
16315092.
16320614.
16324568.
16335585.
16340760.
16342549.
987031.
KEGGilmo:lmo0200.
lmoe:BN418_0210.
PATRICi20309407. VBILisMon69206_0205.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X61210 Genomic DNA. Translation: CAA43524.1 .
M55160 Genomic DNA. Translation: AAA25291.1 .
AJ002742 Genomic DNA. Translation: CAA05714.1 .
AL591974 Genomic DNA. Translation: CAD00727.1 .
PIRi AI1099.
S16890.
RefSeqi NP_463731.1. NC_003210.1.
WP_003722729.1. NC_003210.1.
YP_007602416.1. NC_020557.1.
YP_008273512.1. NC_021823.1.
YP_008274522.1. NC_021824.1.
YP_008279488.1. NC_021825.1.
YP_008281766.1. NC_021826.1.
YP_008282691.1. NC_021827.1.
YP_008298400.1. NC_021838.1.
YP_008303431.1. NC_021839.1.
YP_008305045.1. NC_021840.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1OMI X-ray 2.80 A/B 7-237 [» ]
2BEO X-ray 2.70 A/B 2-237 [» ]
2BGC X-ray 2.30 A/B/D/E/F/G/H/I 2-237 [» ]
ProteinModelPortali P22262.
SMRi P22262. Positions 2-237.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 169963.lmo0200.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAD00727 ; CAD00727 ; CAD00727 .
GeneIDi 14824603.
16308916.
16309966.
16315092.
16320614.
16324568.
16335585.
16340760.
16342549.
987031.
KEGGi lmo:lmo0200.
lmoe:BN418_0210.
PATRICi 20309407. VBILisMon69206_0205.

Organism-specific databases

GenoListi LMO0200.

Phylogenomic databases

eggNOGi COG0664.
OMAi AIAREDC.
OrthoDBi EOG6R87DH.
PhylomeDBi P22262.

Enzyme and pathway databases

BioCyci LMON169963:LMO0200-MONOMER.

Miscellaneous databases

EvolutionaryTracei P22262.

Family and domain databases

Gene3Di 1.10.10.10. 1 hit.
2.60.120.10. 1 hit.
InterProi IPR018490. cNMP-bd-like.
IPR012318. HTH_CRP_2.
IPR014710. RmlC-like_jellyroll.
IPR018335. Tscrpt_reg_HTH_Crp-type_CS.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view ]
SUPFAMi SSF51206. SSF51206. 1 hit.
PROSITEi PS00042. HTH_CRP_1. 1 hit.
PS51063. HTH_CRP_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Pleiotropic control of Listeria monocytogenes virulence factors by a gene that is autoregulated."
    Mengaud J., Dramsi S., Gouin E., Vazquez-Boland J.A., Milon G., Cossart P.
    Mol. Microbiol. 5:2273-2283(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: LO28 / Serovar 1/2c.
  2. "Identification of a gene that positively regulates expression of listeriolysin, the major virulence factor of listeria monocytogenes."
    Leimeister-Waechter M., Haffner C., Domann E., Goebel W., Chakraborty T.
    Proc. Natl. Acad. Sci. U.S.A. 87:8336-8340(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: EGD / Serovar 1/2a.
  3. "A Gly145Ser substitution in the transcriptional activator PrfA causes constitutive overexpression of virulence factors in Listeria monocytogenes."
    Ripio M.T., Dominguez-Bernal G., Lara M., Suarez M., Vazquez-Boland J.A.
    J. Bacteriol. 179:1533-1540(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: P14 / Serovar 4B.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-679 / EGD-e.

Entry informationi

Entry nameiPRFA_LISMO
AccessioniPrimary (citable) accession number: P22262
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: February 1, 1994
Last modified: October 1, 2014
This is version 110 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3