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P22262 (PRFA_LISMO) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 106. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Listeriolysin regulatory protein
Gene names
Name:prfA
Ordered Locus Names:lmo0200
OrganismListeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e) [Reference proteome] [HAMAP]
Taxonomic identifier169963 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesListeriaceaeListeria

Protein attributes

Sequence length237 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Positively regulates expression of listeriolysin, of 1-phosphadidylinositol phosphodiesterase (PI-PLC) and other virulence factors.

Sequence similarities

Contains 1 HTH crp-type DNA-binding domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 237237Listeriolysin regulatory protein
PRO_0000100188

Regions

Domain137 – 21276HTH crp-type

Natural variations

Natural variant1451G → S in prfA* mutant which constitutively overexpresses virulence genes. Presumably blocks prfA in a cofactor-independent transcriptionally active conformation.

Experimental info

Sequence conflict234 – 2363GKL → E Ref.2

Secondary structure

........................................ 237
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P22262 [UniParc].

Last modified February 1, 1994. Version 2.
Checksum: 697542B135158CFB

FASTA23727,301
        10         20         30         40         50         60 
MNAQAEEFKK YLETNGIKPK QFHKKELIFN QWDPQEYCIF LYDGITKLTS ISENGTIMNL 

        70         80         90        100        110        120 
QYYKGAFVIM SGFIDTETSV GYYNLEVISE QATAYVIKIN ELKELLSKNL THFFYVFQTL 

       130        140        150        160        170        180 
QKQVSYSLAK FNDFSINGKL GSICGQLLIL TYVYGKETPD GIKITLDNLT MQELGYSSGI 

       190        200        210        220        230 
AHSSAVSRII SKLKQEKVIV YKNSCFYVQN LDYLKRYAPK LDEWFYLACP ATWGKLN 

« Hide

References

« Hide 'large scale' references
[1]"Pleiotropic control of Listeria monocytogenes virulence factors by a gene that is autoregulated."
Mengaud J., Dramsi S., Gouin E., Vazquez-Boland J.A., Milon G., Cossart P.
Mol. Microbiol. 5:2273-2283(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: LO28 / Serovar 1/2c.
[2]"Identification of a gene that positively regulates expression of listeriolysin, the major virulence factor of listeria monocytogenes."
Leimeister-Waechter M., Haffner C., Domann E., Goebel W., Chakraborty T.
Proc. Natl. Acad. Sci. U.S.A. 87:8336-8340(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: EGD / Serovar 1/2a.
[3]"A Gly145Ser substitution in the transcriptional activator PrfA causes constitutive overexpression of virulence factors in Listeria monocytogenes."
Ripio M.T., Dominguez-Bernal G., Lara M., Suarez M., Vazquez-Boland J.A.
J. Bacteriol. 179:1533-1540(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: P14 / Serovar 4B.
[4]"Comparative genomics of Listeria species."
Glaser P., Frangeul L., Buchrieser C., Rusniok C., Amend A., Baquero F., Berche P., Bloecker H., Brandt P., Chakraborty T., Charbit A., Chetouani F., Couve E., de Daruvar A., Dehoux P., Domann E., Dominguez-Bernal G., Duchaud E. expand/collapse author list , Durant L., Dussurget O., Entian K.-D., Fsihi H., Garcia-del Portillo F., Garrido P., Gautier L., Goebel W., Gomez-Lopez N., Hain T., Hauf J., Jackson D., Jones L.-M., Kaerst U., Kreft J., Kuhn M., Kunst F., Kurapkat G., Madueno E., Maitournam A., Mata Vicente J., Ng E., Nedjari H., Nordsiek G., Novella S., de Pablos B., Perez-Diaz J.-C., Purcell R., Remmel B., Rose M., Schlueter T., Simoes N., Tierrez A., Vazquez-Boland J.-A., Voss H., Wehland J., Cossart P.
Science 294:849-852(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC BAA-679 / EGD-e.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X61210 Genomic DNA. Translation: CAA43524.1.
M55160 Genomic DNA. Translation: AAA25291.1.
AJ002742 Genomic DNA. Translation: CAA05714.1.
AL591974 Genomic DNA. Translation: CAD00727.1.
PIRAI1099.
S16890.
RefSeqNP_463731.1. NC_003210.1.
YP_007602416.1. NC_020557.1.
YP_008273512.1. NC_021823.1.
YP_008274522.1. NC_021824.1.
YP_008279488.1. NC_021825.1.
YP_008281766.1. NC_021826.1.
YP_008282691.1. NC_021827.1.
YP_008298400.1. NC_021838.1.
YP_008303431.1. NC_021839.1.
YP_008305045.1. NC_021840.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1OMIX-ray2.80A/B7-237[»]
2BEOX-ray2.70A/B2-237[»]
2BGCX-ray2.30A/B/D/E/F/G/H/I2-237[»]
ProteinModelPortalP22262.
SMRP22262. Positions 2-237.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING169963.lmo0200.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAD00727; CAD00727; CAD00727.
GeneID14824603.
16308916.
16309966.
16315092.
16320614.
16324568.
16335585.
16340760.
16342549.
987031.
KEGGlmo:lmo0200.
lmoe:BN418_0210.
PATRIC20309407. VBILisMon69206_0205.

Organism-specific databases

GenoListLMO0200.

Phylogenomic databases

eggNOGCOG0664.
OMADICHCEA.
OrthoDBEOG6R87DH.
ProtClustDBCLSK225616.

Enzyme and pathway databases

BioCycLMON169963:LMO0200-MONOMER.

Family and domain databases

Gene3D1.10.10.10. 1 hit.
2.60.120.10. 1 hit.
InterProIPR018490. cNMP-bd-like.
IPR012318. HTH_CRP_2.
IPR014710. RmlC-like_jellyroll.
IPR018335. Tscrpt_reg_HTH_Crp-type_CS.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
SUPFAMSSF51206. SSF51206. 1 hit.
PROSITEPS00042. HTH_CRP_1. 1 hit.
PS51063. HTH_CRP_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP22262.

Entry information

Entry namePRFA_LISMO
AccessionPrimary (citable) accession number: P22262
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: February 1, 1994
Last modified: February 19, 2014
This is version 106 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references