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Protein

4-aminobutyrate aminotransferase GabT

Gene

gabT

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the transfer of the amino group from gamma-aminobutyrate (GABA) to alpha-ketoglutarate (KG) to yield succinic semialdehyde (SSA).1 Publication

Catalytic activityi

4-aminobutanoate + 2-oxoglutarate = succinate semialdehyde + L-glutamate.
(S)-3-amino-2-methylpropanoate + 2-oxoglutarate = 2-methyl-3-oxopropanoate + L-glutamate.

Cofactori

pyridoxal 5'-phosphate1 Publication

Pathwayi: 4-aminobutanoate degradation

This protein is involved in the pathway 4-aminobutanoate degradation, which is part of Amino-acid degradation.
View all proteins of this organism that are known to be involved in the pathway 4-aminobutanoate degradation and in Amino-acid degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei138Pyridoxal phosphate1
Binding sitei297Pyridoxal phosphate1

GO - Molecular functioni

  • (S)-3-amino-2-methylpropionate transaminase activity Source: UniProtKB-EC
  • 4-aminobutyrate transaminase activity Source: EcoCyc
  • identical protein binding Source: GO_Central
  • N2-acetyl-L-ornithine:2-oxoglutarate 5-aminotransferase activity Source: EcoCyc
  • pyridoxal phosphate binding Source: EcoCyc

GO - Biological processi

  • arginine biosynthetic process via ornithine Source: EcoCyc
  • gamma-aminobutyric acid catabolic process Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Aminotransferase, Transferase

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

BioCyciEcoCyc:GABATRANSAM-MONOMER.
ECOL316407:JW2637-MONOMER.
MetaCyc:GABATRANSAM-MONOMER.
BRENDAi2.6.1.19. 2026.
SABIO-RKP22256.
UniPathwayiUPA00733.

Names & Taxonomyi

Protein namesi
Recommended name:
4-aminobutyrate aminotransferase GabT (EC:2.6.1.19)
Alternative name(s):
(S)-3-amino-2-methylpropionate transaminase (EC:2.6.1.22)
GABA aminotransferase
Short name:
GABA-AT
Gamma-amino-N-butyrate transaminase
Short name:
GABA transaminase
Glutamate:succinic semialdehyde transaminase
L-AIBAT
Gene namesi
Name:gabT
Ordered Locus Names:b2662, JW2637
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10361. gabT.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc
Complete GO annotation...

Pathology & Biotechi

Disruption phenotypei

Cells show only 68% of the wild-type activity and are not able to utilize GABA as a nitrogen source.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001203841 – 4264-aminobutyrate aminotransferase GabTAdd BLAST426

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei268N6-(pyridoxal phosphate)lysine1

Proteomic databases

EPDiP22256.
PaxDbiP22256.
PRIDEiP22256.

Expressioni

Inductioni

Induced by RpoS in response to multiple stress conditions, including shifts to acidic pH or high osmolarity as well as starvation or stationary phase. Catabolite repression by glucose (repression relieved by GABA).1 Publication

Interactioni

Subunit structurei

Homotetramer.2 Publications

GO - Molecular functioni

Protein-protein interaction databases

BioGridi4259210. 8 interactors.
DIPiDIP-9725N.
IntActiP22256. 12 interactors.
MINTiMINT-1274800.
STRINGi511145.b2662.

Structurei

Secondary structure

1426
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi4 – 14Combined sources11
Beta strandi22 – 32Combined sources11
Beta strandi34 – 37Combined sources4
Beta strandi42 – 47Combined sources6
Helixi48 – 51Combined sources4
Helixi60 – 69Combined sources10
Turni70 – 72Combined sources3
Turni78 – 80Combined sources3
Helixi84 – 96Combined sources13
Beta strandi103 – 110Combined sources8
Helixi111 – 126Combined sources16
Beta strandi130 – 134Combined sources5
Helixi143 – 148Combined sources6
Turni153 – 158Combined sources6
Beta strandi164 – 169Combined sources6
Helixi174 – 176Combined sources3
Helixi180 – 193Combined sources14
Helixi197 – 199Combined sources3
Beta strandi200 – 205Combined sources6
Turni210 – 213Combined sources4
Helixi219 – 232Combined sources14
Beta strandi235 – 239Combined sources5
Turni241 – 248Combined sources8
Beta strandi249 – 252Combined sources4
Helixi253 – 256Combined sources4
Beta strandi262 – 266Combined sources5
Helixi268 – 271Combined sources4
Beta strandi273 – 275Combined sources3
Beta strandi277 – 282Combined sources6
Helixi283 – 286Combined sources4
Beta strandi297 – 300Combined sources4
Helixi302 – 317Combined sources16
Helixi320 – 340Combined sources21
Beta strandi345 – 351Combined sources7
Beta strandi354 – 360Combined sources7
Helixi361 – 363Combined sources3
Helixi370 – 382Combined sources13
Beta strandi388 – 391Combined sources4
Beta strandi396 – 399Combined sources4
Helixi407 – 424Combined sources18

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1SF2X-ray2.40A/B/C/D1-426[»]
1SFFX-ray1.90A/B/C/D1-426[»]
1SZKX-ray2.52A/B/C/D1-426[»]
1SZSX-ray2.10A/B/C/D1-426[»]
1SZUX-ray2.52A/B/C/D1-426[»]
ProteinModelPortaliP22256.
SMRiP22256.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP22256.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni111 – 112Pyridoxal phosphate binding2
Regioni239 – 242Pyridoxal phosphate binding4

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4108JPW. Bacteria.
COG0160. LUCA.
HOGENOMiHOG000020206.
InParanoidiP22256.
KOiK07250.
OMAiDIFEEEH.
PhylomeDBiP22256.

Family and domain databases

CDDicd00610. OAT_like. 1 hit.
Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR004632. 4NH2But_aminotransferase_bac.
IPR005814. Aminotrans_3.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PANTHERiPTHR11986. PTHR11986. 1 hit.
PfamiPF00202. Aminotran_3. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 1 hit.
TIGRFAMsiTIGR00700. GABAtrnsam. 1 hit.
PROSITEiPS00600. AA_TRANSFER_CLASS_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P22256-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNSNKELMQR RSQAIPRGVG QIHPIFADRA ENCRVWDVEG REYLDFAGGI
60 70 80 90 100
AVLNTGHLHP KVVAAVEAQL KKLSHTCFQV LAYEPYLELC EIMNQKVPGD
110 120 130 140 150
FAKKTLLVTT GSEAVENAVK IARAATKRSG TIAFSGAYHG RTHYTLALTG
160 170 180 190 200
KVNPYSAGMG LMPGHVYRAL YPCPLHGISE DDAIASIHRI FKNDAAPEDI
210 220 230 240 250
AAIVIEPVQG EGGFYASSPA FMQRLRALCD EHGIMLIADE VQSGAGRTGT
260 270 280 290 300
LFAMEQMGVA PDLTTFAKSI AGGFPLAGVT GRAEVMDAVA PGGLGGTYAG
310 320 330 340 350
NPIACVAALE VLKVFEQENL LQKANDLGQK LKDGLLAIAE KHPEIGDVRG
360 370 380 390 400
LGAMIAIELF EDGDHNKPDA KLTAEIVARA RDKGLILLSC GPYYNVLRIL
410 420
VPLTIEDAQI RQGLEIISQC FDEAKQ
Length:426
Mass (Da):45,775
Last modified:August 1, 1991 - v1
Checksum:i02FC80FF0EAA1361
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M88334 Genomic DNA. Translation: AAC36832.1.
U00096 Genomic DNA. Translation: AAC75709.1.
AP009048 Genomic DNA. Translation: BAA16525.1.
PIRiA37846.
RefSeqiNP_417148.1. NC_000913.3.
WP_001087611.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75709; AAC75709; b2662.
BAA16525; BAA16525; BAA16525.
GeneIDi948067.
KEGGiecj:JW2637.
eco:b2662.
PATRICi32120714. VBIEscCol129921_2754.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M88334 Genomic DNA. Translation: AAC36832.1.
U00096 Genomic DNA. Translation: AAC75709.1.
AP009048 Genomic DNA. Translation: BAA16525.1.
PIRiA37846.
RefSeqiNP_417148.1. NC_000913.3.
WP_001087611.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1SF2X-ray2.40A/B/C/D1-426[»]
1SFFX-ray1.90A/B/C/D1-426[»]
1SZKX-ray2.52A/B/C/D1-426[»]
1SZSX-ray2.10A/B/C/D1-426[»]
1SZUX-ray2.52A/B/C/D1-426[»]
ProteinModelPortaliP22256.
SMRiP22256.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259210. 8 interactors.
DIPiDIP-9725N.
IntActiP22256. 12 interactors.
MINTiMINT-1274800.
STRINGi511145.b2662.

Proteomic databases

EPDiP22256.
PaxDbiP22256.
PRIDEiP22256.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75709; AAC75709; b2662.
BAA16525; BAA16525; BAA16525.
GeneIDi948067.
KEGGiecj:JW2637.
eco:b2662.
PATRICi32120714. VBIEscCol129921_2754.

Organism-specific databases

EchoBASEiEB0356.
EcoGeneiEG10361. gabT.

Phylogenomic databases

eggNOGiENOG4108JPW. Bacteria.
COG0160. LUCA.
HOGENOMiHOG000020206.
InParanoidiP22256.
KOiK07250.
OMAiDIFEEEH.
PhylomeDBiP22256.

Enzyme and pathway databases

UniPathwayiUPA00733.
BioCyciEcoCyc:GABATRANSAM-MONOMER.
ECOL316407:JW2637-MONOMER.
MetaCyc:GABATRANSAM-MONOMER.
BRENDAi2.6.1.19. 2026.
SABIO-RKP22256.

Miscellaneous databases

EvolutionaryTraceiP22256.
PROiP22256.

Family and domain databases

CDDicd00610. OAT_like. 1 hit.
Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR004632. 4NH2But_aminotransferase_bac.
IPR005814. Aminotrans_3.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PANTHERiPTHR11986. PTHR11986. 1 hit.
PfamiPF00202. Aminotran_3. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 1 hit.
TIGRFAMsiTIGR00700. GABAtrnsam. 1 hit.
PROSITEiPS00600. AA_TRANSFER_CLASS_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGABT_ECOLI
AccessioniPrimary (citable) accession number: P22256
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: August 1, 1991
Last modified: November 2, 2016
This is version 151 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.