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Protein

3'(2'),5'-bisphosphate nucleotidase CysQ

Gene

cysQ

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Converts 3'(2')-phosphoadenosine 5'-phosphate (PAP) to AMP. May also convert adenosine 3'-phosphate 5'-phosphosulfate (PAPS) to adenosine 5'-phosphosulfate (APS). Has 10000-fold lower activity towards inositol 1,4-bisphosphate (Ins(1,4)P2).2 Publications

Catalytic activityi

Adenosine 3',5'-bisphosphate + H2O = adenosine 5'-phosphate + phosphate.1 Publication

Cofactori

Mg2+1 Publication

Enzyme regulationi

Inhibited by lithium and calcium.1 Publication

Kineticsi

  1. KM=1.1 µM for PAP1 Publication
  2. KM=1.2 mM for Ins(1,4)P21 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Metal bindingi64Magnesium 1By similarity1
    Binding sitei64SubstrateBy similarity1
    Metal bindingi83Magnesium 1By similarity1
    Metal bindingi83Magnesium 2By similarity1
    Metal bindingi85Magnesium 1; via carbonyl oxygenBy similarity1
    Metal bindingi86Magnesium 2By similarity1
    Metal bindingi205Magnesium 2By similarity1
    Binding sitei205SubstrateBy similarity1

    GO - Molecular functioni

    • 3'(2'),5'-bisphosphate nucleotidase activity Source: EcoCyc
    • magnesium ion binding Source: EcoCyc

    GO - Biological processi

    • phosphatidylinositol phosphorylation Source: InterPro
    • sulfate assimilation Source: EcoCyc

    Keywordsi

    Molecular functionHydrolase
    LigandMagnesium, Metal-binding

    Enzyme and pathway databases

    BioCyciEcoCyc:EG10043-MONOMER.
    MetaCyc:EG10043-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    3'(2'),5'-bisphosphate nucleotidase CysQ (EC:3.1.3.7)
    Alternative name(s):
    3'(2'),5-bisphosphonucleoside 3'(2')-phosphohydrolase
    3'-phosphoadenosine 5'-phosphate phosphatase
    Short name:
    PAP phosphatase
    DPNPase
    Gene namesi
    Name:cysQ
    Synonyms:amtA
    Ordered Locus Names:b4214, JW4172
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
    Proteomesi
    • UP000000318 Componenti: Chromosome
    • UP000000625 Componenti: Chromosome

    Organism-specific databases

    EcoGeneiEG10043. cysQ.

    Subcellular locationi

    GO - Cellular componenti

    Keywords - Cellular componenti

    Cell inner membrane, Cell membrane, Cytoplasm, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00001425411 – 2463'(2'),5'-bisphosphate nucleotidase CysQAdd BLAST246

    Proteomic databases

    PaxDbiP22255.
    PRIDEiP22255.

    Expressioni

    Inductioni

    Strongly repressed during nitrogen excess.

    Interactioni

    Protein-protein interaction databases

    BioGridi4259304. 19 interactors.
    DIPiDIP-9385N.
    IntActiP22255. 6 interactors.
    MINTiMINT-1300047.
    STRINGi316407.85676965.

    Structurei

    3D structure databases

    ProteinModelPortaliP22255.
    SMRiP22255.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni85 – 88Substrate bindingBy similarity4

    Sequence similaritiesi

    Belongs to the inositol monophosphatase family.Curated

    Phylogenomic databases

    eggNOGiENOG4108RNI. Bacteria.
    COG1218. LUCA.
    HOGENOMiHOG000282237.
    InParanoidiP22255.
    KOiK01082.
    PhylomeDBiP22255.

    Family and domain databases

    InterProiView protein in InterPro
    IPR006240. Bisphos_bac.
    IPR020583. Inositol_monoP_metal-BS.
    IPR000760. Inositol_monophosphatase-like.
    IPR020550. Inositol_monophosphatase_CS.
    PfamiView protein in Pfam
    PF00459. Inositol_P. 1 hit.
    TIGRFAMsiTIGR01331. bisphos_cysQ. 1 hit.
    PROSITEiView protein in PROSITE
    PS00629. IMP_1. 1 hit.
    PS00630. IMP_2. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P22255-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MLDQVCQLAR NAGDAIMQVY DGTKPMDVVS KADNSPVTAA DIAAHTVIMD
    60 70 80 90 100
    GLRTLTPDVP VLSEEDPPGW EVRQHWQRYW LVDPLDGTKE FIKRNGEFTV
    110 120 130 140 150
    NIALIDHGKP ILGVVYAPVM NVMYSAAEGK AWKEECGVRK QIQVRDARPP
    160 170 180 190 200
    LVVISRSHAD AELKEYLQQL GEHQTTSIGS SLKFCLVAEG QAQLYPRFGP
    210 220 230 240
    TNIWDTAAGH AVAAAAGAHV HDWQGKPLDY TPRESFLNPG FRVSIY
    Length:246
    Mass (Da):27,176
    Last modified:June 20, 2003 - v2
    Checksum:iF0CBDFAA98DDAF85
    GO

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti193 – 194QL → HV (PubMed:1856684).Curated2
    Sequence conflicti193 – 194QL → HV (PubMed:1729235).Curated2

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    M55170 Genomic DNA. Translation: AAA23444.1.
    M80795 Genomic DNA. Translation: AAA23657.1.
    U14003 Genomic DNA. Translation: AAA97110.1.
    U00096 Genomic DNA. Translation: AAC77171.1.
    AP009048 Genomic DNA. Translation: BAE78215.1.
    X54008 Genomic DNA. Translation: CAA37953.1.
    PIRiS56439.
    RefSeqiNP_418635.1. NC_000913.3.
    WP_000886919.1. NZ_LN832404.1.

    Genome annotation databases

    EnsemblBacteriaiAAC77171; AAC77171; b4214.
    BAE78215; BAE78215; BAE78215.
    GeneIDi948728.
    KEGGiecj:JW4172.
    eco:b4214.
    PATRICifig|1411691.4.peg.2487.

    Similar proteinsi

    Entry informationi

    Entry nameiCYSQ_ECOLI
    AccessioniPrimary (citable) accession number: P22255
    Secondary accession number(s): Q2M691
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1991
    Last sequence update: June 20, 2003
    Last modified: August 30, 2017
    This is version 143 of the entry and version 2 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Caution

    Was originally thought to be an ammonium transport protein.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. SIMILARITY comments
      Index of protein domains and families