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Protein

3'(2'),5'-bisphosphate nucleotidase CysQ

Gene

cysQ

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Converts adenosine-3',5'-bisphosphate (PAP) to AMP. May also convert adenosine 3'-phosphate 5'-phosphosulfate (PAPS) to adenosine 5'-phosphosulfate (APS). Has 10000-fold lower activity towards inositol 1,4-bisphosphate (Ins(1,4)P2).3 Publications

Caution

Was originally thought to be an ammonium transport protein.1 Publication

Catalytic activityi

Adenosine 3',5'-bisphosphate + H2O = adenosine 5'-phosphate + phosphate.UniRule annotation1 Publication

Cofactori

Mg2+UniRule annotation1 Publication

Enzyme regulationi

Inhibited by lithium and calcium.1 Publication

Kineticsi

  1. KM=1.1 µM for PAP1 Publication
  2. KM=1.2 mM for Ins(1,4)P21 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Metal bindingi64Magnesium 1UniRule annotationBy similarity1
    Binding sitei64SubstrateUniRule annotationBy similarity1
    Metal bindingi83Magnesium 1UniRule annotationBy similarity1
    Metal bindingi83Magnesium 2UniRule annotationBy similarity1
    Metal bindingi85Magnesium 1; via carbonyl oxygenUniRule annotationBy similarity1
    Metal bindingi86Magnesium 2UniRule annotationBy similarity1
    Metal bindingi205Magnesium 2UniRule annotationBy similarity1
    Binding sitei205SubstrateUniRule annotationBy similarity1

    GO - Molecular functioni

    • 3'(2'),5'-bisphosphate nucleotidase activity Source: EcoCyc
    • magnesium ion binding Source: EcoCyc

    GO - Biological processi

    • phosphatidylinositol phosphorylation Source: InterPro
    • sulfate assimilation Source: EcoCyc

    Keywordsi

    Molecular functionHydrolase
    LigandMagnesium, Metal-binding

    Enzyme and pathway databases

    BioCyciEcoCyc:EG10043-MONOMER
    MetaCyc:EG10043-MONOMER

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    3'(2'),5'-bisphosphate nucleotidase CysQUniRule annotationCurated (EC:3.1.3.7UniRule annotation1 Publication)
    Alternative name(s):
    3'(2'),5-bisphosphonucleoside 3'(2')-phosphohydrolaseUniRule annotationCurated
    3'-phosphoadenosine 5'-phosphate phosphataseUniRule annotationCurated
    Short name:
    PAP phosphataseUniRule annotationCurated
    DPNPaseCurated
    Gene namesi
    Name:cysQ1 PublicationUniRule annotation
    Synonyms:amtA1 Publication
    Ordered Locus Names:b4214, JW4172
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
    Proteomesi
    • UP000000318 Componenti: Chromosome
    • UP000000625 Componenti: Chromosome

    Organism-specific databases

    EcoGeneiEG10043 cysQ

    Subcellular locationi

    GO - Cellular componenti

    Keywords - Cellular componenti

    Cell inner membrane, Cell membrane, Membrane

    Pathology & Biotechi

    Disruption phenotypei

    Mutants require cysteine or sulfite to grow under aerobic conditions.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00001425411 – 2463'(2'),5'-bisphosphate nucleotidase CysQAdd BLAST246

    Proteomic databases

    EPDiP22255
    PaxDbiP22255
    PRIDEiP22255

    Expressioni

    Inductioni

    Strongly repressed during nitrogen excess.

    Interactioni

    Protein-protein interaction databases

    BioGridi4259304, 39 interactors
    DIPiDIP-9385N
    IntActiP22255, 6 interactors
    STRINGi316407.85676965

    Structurei

    3D structure databases

    ProteinModelPortaliP22255
    SMRiP22255
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni85 – 88Substrate bindingUniRule annotationBy similarity4

    Sequence similaritiesi

    Belongs to the inositol monophosphatase superfamily. CysQ family.UniRule annotationCurated

    Phylogenomic databases

    eggNOGiENOG4108RNI Bacteria
    COG1218 LUCA
    HOGENOMiHOG000282237
    InParanoidiP22255
    KOiK01082
    OMAiKDWDMAA
    PhylomeDBiP22255

    Family and domain databases

    HAMAPiMF_02095 CysQ, 1 hit
    InterProiView protein in InterPro
    IPR006240 CysQ
    IPR020583 Inositol_monoP_metal-BS
    IPR000760 Inositol_monophosphatase-like
    IPR020550 Inositol_monophosphatase_CS
    PfamiView protein in Pfam
    PF00459 Inositol_P, 1 hit
    TIGRFAMsiTIGR01331 bisphos_cysQ, 1 hit
    PROSITEiView protein in PROSITE
    PS00629 IMP_1, 1 hit
    PS00630 IMP_2, 1 hit

    Sequencei

    Sequence statusi: Complete.

    P22255-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MLDQVCQLAR NAGDAIMQVY DGTKPMDVVS KADNSPVTAA DIAAHTVIMD
    60 70 80 90 100
    GLRTLTPDVP VLSEEDPPGW EVRQHWQRYW LVDPLDGTKE FIKRNGEFTV
    110 120 130 140 150
    NIALIDHGKP ILGVVYAPVM NVMYSAAEGK AWKEECGVRK QIQVRDARPP
    160 170 180 190 200
    LVVISRSHAD AELKEYLQQL GEHQTTSIGS SLKFCLVAEG QAQLYPRFGP
    210 220 230 240
    TNIWDTAAGH AVAAAAGAHV HDWQGKPLDY TPRESFLNPG FRVSIY
    Length:246
    Mass (Da):27,176
    Last modified:June 20, 2003 - v2
    Checksum:iF0CBDFAA98DDAF85
    GO

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti193 – 194QL → HV (PubMed:1856684).Curated2
    Sequence conflicti193 – 194QL → HV (PubMed:1729235).Curated2

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    M55170 Genomic DNA Translation: AAA23444.1
    M80795 Genomic DNA Translation: AAA23657.1
    U14003 Genomic DNA Translation: AAA97110.1
    U00096 Genomic DNA Translation: AAC77171.1
    AP009048 Genomic DNA Translation: BAE78215.1
    X54008 Genomic DNA Translation: CAA37953.1
    PIRiS56439
    RefSeqiNP_418635.1, NC_000913.3
    WP_000886919.1, NZ_LN832404.1

    Genome annotation databases

    EnsemblBacteriaiAAC77171; AAC77171; b4214
    BAE78215; BAE78215; BAE78215
    GeneIDi948728
    KEGGiecj:JW4172
    eco:b4214
    PATRICifig|1411691.4.peg.2487

    Similar proteinsi

    Entry informationi

    Entry nameiCYSQ_ECOLI
    AccessioniPrimary (citable) accession number: P22255
    Secondary accession number(s): Q2M691
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1991
    Last sequence update: June 20, 2003
    Last modified: May 23, 2018
    This is version 149 of the entry and version 2 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

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