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Protein

3'(2'),5'-bisphosphate nucleotidase CysQ

Gene

cysQ

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Converts 3'(2')-phosphoadenosine 5'-phosphate (PAP) to AMP. May also convert adenosine 3'-phosphate 5'-phosphosulfate (PAPS) to adenosine 5'-phosphosulfate (APS). Has 10000-fold lower activity towards inositol 1,4-bisphosphate (Ins(1,4)P2).2 Publications

Catalytic activityi

Adenosine 3',5'-bisphosphate + H2O = adenosine 5'-phosphate + phosphate.1 Publication

Cofactori

Mg2+1 Publication

Enzyme regulationi

Inhibited by lithium and calcium.1 Publication

Kineticsi

  1. KM=1.1 µM for PAP1 Publication
  2. KM=1.2 mM for Ins(1,4)P21 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi64 – 641Magnesium 1By similarity
    Binding sitei64 – 641SubstrateBy similarity
    Metal bindingi83 – 831Magnesium 1By similarity
    Metal bindingi83 – 831Magnesium 2By similarity
    Metal bindingi85 – 851Magnesium 1; via carbonyl oxygenBy similarity
    Metal bindingi86 – 861Magnesium 2By similarity
    Metal bindingi205 – 2051Magnesium 2By similarity
    Binding sitei205 – 2051SubstrateBy similarity

    GO - Molecular functioni

    • 3'(2'),5'-bisphosphate nucleotidase activity Source: EcoCyc
    • magnesium ion binding Source: EcoCyc

    GO - Biological processi

    • phosphatidylinositol phosphorylation Source: InterPro
    • sulfate assimilation Source: EcoCyc
    Complete GO annotation...

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Ligandi

    Magnesium, Metal-binding

    Enzyme and pathway databases

    BioCyciEcoCyc:EG10043-MONOMER.
    ECOL316407:JW4172-MONOMER.
    MetaCyc:EG10043-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    3'(2'),5'-bisphosphate nucleotidase CysQ (EC:3.1.3.7)
    Alternative name(s):
    3'(2'),5-bisphosphonucleoside 3'(2')-phosphohydrolase
    3'-phosphoadenosine 5'-phosphate phosphatase
    Short name:
    PAP phosphatase
    DPNPase
    Gene namesi
    Name:cysQ
    Synonyms:amtA
    Ordered Locus Names:b4214, JW4172
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    Proteomesi
    • UP000000318 Componenti: Chromosome
    • UP000000625 Componenti: Chromosome

    Organism-specific databases

    EcoGeneiEG10043. cysQ.

    Subcellular locationi

    GO - Cellular componenti

    Complete GO annotation...

    Keywords - Cellular componenti

    Cell inner membrane, Cell membrane, Cytoplasm, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 2462463'(2'),5'-bisphosphate nucleotidase CysQPRO_0000142541Add
    BLAST

    Proteomic databases

    EPDiP22255.
    PaxDbiP22255.
    PRIDEiP22255.

    Expressioni

    Inductioni

    Strongly repressed during nitrogen excess.

    Interactioni

    Protein-protein interaction databases

    BioGridi4259304. 19 interactions.
    DIPiDIP-9385N.
    IntActiP22255. 6 interactions.
    MINTiMINT-1300047.
    STRINGi511145.b4214.

    Structurei

    3D structure databases

    ProteinModelPortaliP22255.
    SMRiP22255. Positions 9-243.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni85 – 884Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the inositol monophosphatase family.Curated

    Phylogenomic databases

    eggNOGiENOG4108RNI. Bacteria.
    COG1218. LUCA.
    HOGENOMiHOG000282237.
    InParanoidiP22255.
    KOiK01082.
    OMAiAIMQVYN.
    PhylomeDBiP22255.

    Family and domain databases

    InterProiIPR006240. Bisphos_bac.
    IPR020583. Inositol_monoP_metal-BS.
    IPR000760. Inositol_monophosphatase.
    IPR020550. Inositol_monophosphatase_CS.
    [Graphical view]
    PANTHERiPTHR20854. PTHR20854. 1 hit.
    PfamiPF00459. Inositol_P. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR01331. bisphos_cysQ. 1 hit.
    PROSITEiPS00629. IMP_1. 1 hit.
    PS00630. IMP_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P22255-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MLDQVCQLAR NAGDAIMQVY DGTKPMDVVS KADNSPVTAA DIAAHTVIMD
    60 70 80 90 100
    GLRTLTPDVP VLSEEDPPGW EVRQHWQRYW LVDPLDGTKE FIKRNGEFTV
    110 120 130 140 150
    NIALIDHGKP ILGVVYAPVM NVMYSAAEGK AWKEECGVRK QIQVRDARPP
    160 170 180 190 200
    LVVISRSHAD AELKEYLQQL GEHQTTSIGS SLKFCLVAEG QAQLYPRFGP
    210 220 230 240
    TNIWDTAAGH AVAAAAGAHV HDWQGKPLDY TPRESFLNPG FRVSIY
    Length:246
    Mass (Da):27,176
    Last modified:June 20, 2003 - v2
    Checksum:iF0CBDFAA98DDAF85
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti193 – 1942QL → HV (PubMed:1856684).Curated
    Sequence conflicti193 – 1942QL → HV (PubMed:1729235).Curated

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    M55170 Genomic DNA. Translation: AAA23444.1.
    M80795 Genomic DNA. Translation: AAA23657.1.
    U14003 Genomic DNA. Translation: AAA97110.1.
    U00096 Genomic DNA. Translation: AAC77171.1.
    AP009048 Genomic DNA. Translation: BAE78215.1.
    X54008 Genomic DNA. Translation: CAA37953.1.
    PIRiS56439.
    RefSeqiNP_418635.1. NC_000913.3.
    WP_000886919.1. NZ_LN832404.1.

    Genome annotation databases

    EnsemblBacteriaiAAC77171; AAC77171; b4214.
    BAE78215; BAE78215; BAE78215.
    GeneIDi948728.
    KEGGiecj:JW4172.
    eco:b4214.
    PATRICi32124003. VBIEscCol129921_4346.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    M55170 Genomic DNA. Translation: AAA23444.1.
    M80795 Genomic DNA. Translation: AAA23657.1.
    U14003 Genomic DNA. Translation: AAA97110.1.
    U00096 Genomic DNA. Translation: AAC77171.1.
    AP009048 Genomic DNA. Translation: BAE78215.1.
    X54008 Genomic DNA. Translation: CAA37953.1.
    PIRiS56439.
    RefSeqiNP_418635.1. NC_000913.3.
    WP_000886919.1. NZ_LN832404.1.

    3D structure databases

    ProteinModelPortaliP22255.
    SMRiP22255. Positions 9-243.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi4259304. 19 interactions.
    DIPiDIP-9385N.
    IntActiP22255. 6 interactions.
    MINTiMINT-1300047.
    STRINGi511145.b4214.

    Proteomic databases

    EPDiP22255.
    PaxDbiP22255.
    PRIDEiP22255.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiAAC77171; AAC77171; b4214.
    BAE78215; BAE78215; BAE78215.
    GeneIDi948728.
    KEGGiecj:JW4172.
    eco:b4214.
    PATRICi32124003. VBIEscCol129921_4346.

    Organism-specific databases

    EchoBASEiEB0041.
    EcoGeneiEG10043. cysQ.

    Phylogenomic databases

    eggNOGiENOG4108RNI. Bacteria.
    COG1218. LUCA.
    HOGENOMiHOG000282237.
    InParanoidiP22255.
    KOiK01082.
    OMAiAIMQVYN.
    PhylomeDBiP22255.

    Enzyme and pathway databases

    BioCyciEcoCyc:EG10043-MONOMER.
    ECOL316407:JW4172-MONOMER.
    MetaCyc:EG10043-MONOMER.

    Miscellaneous databases

    PROiP22255.

    Family and domain databases

    InterProiIPR006240. Bisphos_bac.
    IPR020583. Inositol_monoP_metal-BS.
    IPR000760. Inositol_monophosphatase.
    IPR020550. Inositol_monophosphatase_CS.
    [Graphical view]
    PANTHERiPTHR20854. PTHR20854. 1 hit.
    PfamiPF00459. Inositol_P. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR01331. bisphos_cysQ. 1 hit.
    PROSITEiPS00629. IMP_1. 1 hit.
    PS00630. IMP_2. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Entry informationi

    Entry nameiCYSQ_ECOLI
    AccessioniPrimary (citable) accession number: P22255
    Secondary accession number(s): Q2M691
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1991
    Last sequence update: June 20, 2003
    Last modified: September 7, 2016
    This is version 136 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Caution

    Was originally thought to be an ammonium transport protein.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.