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P22248

- GLNA_AZOVI

UniProt

P22248 - GLNA_AZOVI

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Protein

Glutamine synthetase

Gene

glnA

Organism
Azotobacter vinelandii
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.

Enzyme regulationi

The activity of this enzyme is controlled by adenylation under conditions of abundant glutamine. The fully adenylated enzyme complex is inactive (By similarity).By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. glutamate-ammonia ligase activity Source: UniProtKB-EC

GO - Biological processi

  1. glutamine biosynthetic process Source: InterPro
  2. nitrogen fixation Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Nitrogen fixation

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine synthetase (EC:6.3.1.2)
Alternative name(s):
Glutamate--ammonia ligase
Gene namesi
Name:glnA
OrganismiAzotobacter vinelandii
Taxonomic identifieri354 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaeAzotobacter

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 467467Glutamine synthetasePRO_0000153231Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei396 – 3961O-AMP-tyrosineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiP22248.

Interactioni

Subunit structurei

Oligomer of 12 subunits arranged in the form of two hexagons.

Structurei

3D structure databases

ProteinModelPortaliP22248.
SMRiP22248. Positions 5-467.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glutamine synthetase family.Curated

Family and domain databases

Gene3Di3.10.20.70. 1 hit.
3.30.590.10. 1 hit.
InterProiIPR008147. Gln_synt_beta.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR004809. Gln_synth_I.
IPR001637. Gln_synth_I_adenylation_site.
IPR027302. Gln_synth_N_conserv_site.
[Graphical view]
PfamiPF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
[Graphical view]
SUPFAMiSSF54368. SSF54368. 1 hit.
TIGRFAMsiTIGR00653. GlnA. 1 hit.
PROSITEiPS00180. GLNA_1. 1 hit.
PS00182. GLNA_ADENYLATION. 1 hit.
PS00181. GLNA_ATP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P22248-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSKSLQLIKE HDVKWIDLRF TDTKGKQQHV TMPARDVDDD FFEYGKMFDG
60 70 80 90 100
SSIAGWKGIE ASDMILMPDD STAVLDPFTE EPTLIIVCDI IEPSTMQGYD
110 120 130 140 150
RDPRAIARRA EEYLKSTGIG DTAFFGPEPE FFIFDEVKYK SDISGSMFKI
160 170 180 190 200
FSEQAAWNTD ADFEGGNKGH RPGVKGGYFP VPPVDHDHEI RTAMCNALEE
210 220 230 240 250
MGLKVEVHHH EVATAGQNEI GVSFNTLVAK ADEVQTLKYC VHNVADAYGK
260 270 280 290 300
TVTFMPKPLY GDNGSGMHVH MSIAKDGKNT FAGEGYAGLS DTALYFIGGI
310 320 330 340 350
IKHGKALNGF TNPSTNSYKR LVPGFEAPVM LAYSARNRSA SIRIPYVNSP
360 370 380 390 400
KARRIEARFP DPSANPYLAF AALLMAGLDG IQNKIHPGDA ADKNLYDLPP
410 420 430 440 450
EEAKEIPQVC GSLKEALEEL DKGRAFLTKG GVFSDDFIDA YLELKSEEEI
460
KVRTFVHPLE YDLYYSV
Length:467
Mass (Da):51,746
Last modified:August 1, 1991 - v1
Checksum:iD4EFA6EC895E38AF
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M57275 Genomic DNA. Translation: AAA62673.1.
PIRiA37153. AJAVQ.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M57275 Genomic DNA. Translation: AAA62673.1 .
PIRi A37153. AJAVQ.

3D structure databases

ProteinModelPortali P22248.
SMRi P22248. Positions 5-467.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PRIDEi P22248.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.10.20.70. 1 hit.
3.30.590.10. 1 hit.
InterProi IPR008147. Gln_synt_beta.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR004809. Gln_synth_I.
IPR001637. Gln_synth_I_adenylation_site.
IPR027302. Gln_synth_N_conserv_site.
[Graphical view ]
Pfami PF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
[Graphical view ]
SUPFAMi SSF54368. SSF54368. 1 hit.
TIGRFAMsi TIGR00653. GlnA. 1 hit.
PROSITEi PS00180. GLNA_1. 1 hit.
PS00182. GLNA_ADENYLATION. 1 hit.
PS00181. GLNA_ATP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Molecular analysis of the Azotobacter vinelandii glnA gene encoding glutamine synthetase."
    Toukdarian A., Saunders G., Selman-Sosa G., Santero E., Woodley P., Kennedy C.
    J. Bacteriol. 172:6529-6539(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiGLNA_AZOVI
AccessioniPrimary (citable) accession number: P22248
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: August 1, 1991
Last modified: October 29, 2014
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3