Reviewed, UniProtKB/Swiss-Prot P22244 (RIP1_HORVU)

Names and origin

Protein names

Recommended name:
    Protein synthesis inhibitor I
    EC=3.2.2.22
Alternative name(s):
    Ribosome-inactivating protein I
    rRNA N-glycosidase

Gene names

Name:

RIP30

Organism

Hordeum vulgare (Barley)

Taxonomic identifier

4513 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Pooideae › Triticeae › Hordeum

Protein attributes

Sequence length	281 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Inhibits the elongation phase of protein synthesis. It inactivates fungal ribosomes even more effectively than mammalian ribosomes and is thought to function as a constitutive antifungal agent in plants.
Catalytic activity	Endohydrolysis of the N-glycosidic bond at one specific adenosine on the 28S rRNA.
Subcellular location	Cytoplasm. Note: Starchy endosperm of mature seeds.
Miscellaneous	Three similar RIP 30 isoforms I, II, and III have been described in Barley.
Sequence similarities	Belongs to the ribosome-inactivating protein family. Type 1 RIP subfamily.

Ontologies

Keywords
Biological process	Plant defense
Cellular component	Cytoplasm
Molecular function	Antimicrobial Fungicide Hydrolase Protein synthesis inhibitor Toxin
PTM	Acetylation
Gene Ontology (GO)
Biological process	defense response to fungus Inferred from electronic annotation. Source: UniProtKB-KW negative regulation of translation Inferred from electronic annotation. Source: UniProtKB-KW pathogenesis Inferred from electronic annotation. Source: UniProtKB-KW xenobiotic metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	rRNA N-glycosylase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 281	280	Protein synthesis inhibitor I		PRO_0000221401
Sites
	Active site	175	1	By similarity
Amino acid modifications
	Modified residue	2	1	N-acetylalanine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P22244-1 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: DD7B49FD08EA94F8	FASTA	281	29,973
	10 20 30 40 50 60 MAAKMAKNVD KPLFTATFNV QASSADYATF IAGIRNKLRN PAHFSHNRPV LPPVEPNVPP 70 80 90 100 110 120 SRWFHVVLKA SPTSAGLTLA IRADNIYLEG FKSSDGTWWE LTPGLIPGAT YVGFGGTYRD 130 140 150 160 170 180 LLGDTDKLTN VALGRQQLAD AVTALHGRTK ADKPSGPKQQ QAREAVTTLL LMVNEATRFQ 190 200 210 220 230 240 TVSGFVAGLL HPKAVEKKSG KIGNEMKAQV NGWQDLSAAL LKTDVKPPPG KSPAKFAPIE 250 260 270 280 KMGVRTAVQA ANTLGILLFV EVPGGLTVAK ALELFHASGG K

References

[1]	"Biochemical and molecular characterization of three barley seed proteins with antifungal properties." Leah R., Tommerup H., Svendsen I., Mundy J. J. Biol. Chem. 266:1564-1573(1991) [PubMed: 1899089] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Piggy.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M62905 mRNA. Translation: AAA32950.1.
PIR	B38664.
UniGene	Hv.324
3D structure databases
ModBase	Search...
Organism-specific databases
Gramene	P22244.
Enzyme and pathway databases
BRENDA	3.2.2.22. 283.
Family and domain databases
InterPro	IPR001574. Ribosome_inactivat_prot. IPR017988. Ribosome_inactivat_prot_CS. IPR016138. Ribosome_inactivat_prot_sub1. IPR017989. Ribosome_inactivat_prot_subgr. [Graphical view]
Gene3D	G3DSA:3.40.420.10. Ribosome_inactivat_prot_sub1. 1 hit.
Pfam	PF00161. RIP. 1 hit. [Graphical view]
PRINTS	PR00396. SHIGARICIN.
PROSITE	PS00275. SHIGA_RICIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RIP1_HORVU

Accession

Primary (citable) accession number: P22244

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1991
Last sequence update:	January 23, 2007
Last modified:	June 16, 2009
This is version 56 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)