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Protein

Zinc finger protein 42

Gene

Zfp42

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the reprogramming of X-chromosome inactivation during the acquisition of pluripotency. Required for efficient elongation of TSIX, a non-coding RNA antisense to XIST. Binds DXPas34 enhancer within the TSIX promoter. Involved in ES cell self-renewal.2 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri170 – 19425C2H2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri199 – 22123C2H2-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri227 – 25125C2H2-type 3PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri258 – 28124C2H2-type 4PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • sequence-specific DNA binding Source: MGI

GO - Biological processi

  • in utero embryonic development Source: MGI
  • regulation of genetic imprinting Source: MGI
  • regulation of transcription, DNA-templated Source: UniProtKB-KW
  • spermatid development Source: MGI
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Zinc finger protein 42
Short name:
Zfp-42
Alternative name(s):
Reduced expression protein 1
Short name:
REX-1
Short name:
mREX-1
Gene namesi
Name:Zfp42
Synonyms:Rex-1, Rex1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:99187. Zfp42.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: MGI
  • nucleus Source: MGI
  • protein complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 288288Zinc finger protein 42PRO_0000047300Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki213 – 213Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication
Cross-linki215 – 215Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication

Post-translational modificationi

Polyubiquitinated by RNF12, leading to proteasomal degradation.1 Publication

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Proteomic databases

PaxDbiP22227.
PeptideAtlasiP22227.
PRIDEiP22227.

PTM databases

iPTMnetiP22227.
PhosphoSiteiP22227.

Expressioni

Tissue specificityi

Restricted to testis, to germ cells in the early stages of spermatogenesis. Not expressed in spermatids, nor spermatozoa. Expressed in embryonic stem (ES) cells.4 Publications

Developmental stagei

Expressed in the inner cell mass of 4.5 dpc blastocysts, as well as in the polar trophoblast. At 6 dpc, abundant in the extraembryonic ectoderm and the ectoplacental cone. At this stage, not detected in the embryonic ectoderm. At 7 dpc, restricted to the extraembryonic ectoderm and the ectoplacental cone. Also expressed in the placenta. Expressed in male germ cells undergoing meiosis.1 Publication

Inductioni

Up-regulated by NANOG, up-regulation which is increased by SOX2 and POU5F1. Up-regulated by nicotine; up-regulation which is prevented with tubocurarine, a nicotinic acetylcholine receptor antagonist. Down-regulated by sodium vanadate and retinoic acid (RA). In ES cells, down-regulation correlates with differentiation.5 Publications

Gene expression databases

CleanExiMM_ZFP42.

Interactioni

Protein-protein interaction databases

BioGridi204664. 16 interactions.
DIPiDIP-54911N.
IntActiP22227. 2 interactions.
STRINGi10090.ENSMUSP00000080765.

Structurei

3D structure databases

ProteinModelPortaliP22227.
SMRiP22227. Positions 119-162, 170-282.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi56 – 10348Asp/Glu-rich (acidic)Add
BLAST

Sequence similaritiesi

Contains 4 C2H2-type zinc fingers.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri170 – 19425C2H2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri199 – 22123C2H2-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri227 – 25125C2H2-type 3PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri258 – 28124C2H2-type 4PROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1721. Eukaryota.
COG5048. LUCA.
HOGENOMiHOG000008232.
HOVERGENiHBG006823.
InParanoidiP22227.
KOiK11516.
PhylomeDBiP22227.

Family and domain databases

Gene3Di3.30.160.60. 4 hits.
InterProiIPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
SMARTiSM00355. ZnF_C2H2. 4 hits.
[Graphical view]
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 4 hits.
PS50157. ZINC_FINGER_C2H2_2. 4 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P22227-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNEQKMNEQM KKTAKTSGQK GPGGRALDRL TLKQDEARPV QNTRVEAPRV
60 70 80 90 100
TYTIRDESEI SPETEEDGFP DGYLECIIRG EFSEPILEED FLFKSFESLE
110 120 130 140 150
EVEQNLSRQV LEASSLLESS LEYMTKGTKQ EKTEVTQETP PLRVGASSLL
160 170 180 190 200
AGGPAEKPEG GVYCGVLSML ECPQAGCKKK LRGKTALRKH MLVHGPRRHV
210 220 230 240 250
CAECGKAFTE SSKLKRHFLV HTGEKPYQCT FEGCGKRFSL DFNLRTHIRI
260 270 280
HTGERRFVCP FDGCEKSFIQ SNNQKIHILT HAKAGKKC
Length:288
Mass (Da):32,398
Last modified:August 1, 1991 - v1
Checksum:i4B1ABFDF372A7D7B
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti13 – 131T → A in AAH53399 (PubMed:15489334).Curated
Sequence conflicti133 – 1331T → R in AAH53399 (PubMed:15489334).Curated
Sequence conflicti155 – 1551A → T in AAH53399 (PubMed:15489334).Curated
Sequence conflicti183 – 1831G → D in AAH53399 (PubMed:15489334).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M28382 mRNA. Translation: AAA40053.1.
M97812 Genomic DNA. Translation: AAA19700.1.
BC053399 mRNA. Translation: AAH53399.1.
BC098467 mRNA. Translation: AAH98467.1.
CCDSiCCDS22267.1.
PIRiA33828.
RefSeqiNP_033582.2. NM_009556.3.
UniGeneiMm.285848.

Genome annotation databases

GeneIDi22702.
KEGGimmu:22702.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M28382 mRNA. Translation: AAA40053.1.
M97812 Genomic DNA. Translation: AAA19700.1.
BC053399 mRNA. Translation: AAH53399.1.
BC098467 mRNA. Translation: AAH98467.1.
CCDSiCCDS22267.1.
PIRiA33828.
RefSeqiNP_033582.2. NM_009556.3.
UniGeneiMm.285848.

3D structure databases

ProteinModelPortaliP22227.
SMRiP22227. Positions 119-162, 170-282.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi204664. 16 interactions.
DIPiDIP-54911N.
IntActiP22227. 2 interactions.
STRINGi10090.ENSMUSP00000080765.

PTM databases

iPTMnetiP22227.
PhosphoSiteiP22227.

Proteomic databases

PaxDbiP22227.
PeptideAtlasiP22227.
PRIDEiP22227.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi22702.
KEGGimmu:22702.

Organism-specific databases

CTDi132625.
MGIiMGI:99187. Zfp42.

Phylogenomic databases

eggNOGiKOG1721. Eukaryota.
COG5048. LUCA.
HOGENOMiHOG000008232.
HOVERGENiHBG006823.
InParanoidiP22227.
KOiK11516.
PhylomeDBiP22227.

Miscellaneous databases

PROiP22227.
SOURCEiSearch...

Gene expression databases

CleanExiMM_ZFP42.

Family and domain databases

Gene3Di3.30.160.60. 4 hits.
InterProiIPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
SMARTiSM00355. ZnF_C2H2. 4 hits.
[Graphical view]
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 4 hits.
PS50157. ZINC_FINGER_C2H2_2. 4 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiZFP42_MOUSE
AccessioniPrimary (citable) accession number: P22227
Secondary accession number(s): Q4KMN3, Q7TPU3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: August 1, 1991
Last modified: September 7, 2016
This is version 137 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.