P22224 (SEC15_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Exocyst complex component SEC15

Gene names

Name:	SEC15
Ordered Locus Names:	YGL233W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	910 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of the exocyst complex involved in the docking of exocytic vesicles with fusion sites on the plasma membrane. Ref.4
Subunit structure	The exocyst complex is composed of SEC3, SEC5, SEC6, SEC8, SEC10, SEC15, EXO70 and EXO84. Interacts with SEC4.
Subcellular location	Cytoplasm. Cell membrane; Peripheral membrane protein. Note: 25% cytoplasmic. 75% is associated with the cell membrane.
Miscellaneous	Present with 4280 molecules/cell in log phase SD medium. Ref.5
Sequence similarities	Belongs to the SEC15 family.

Ontologies

Keywords
Biological process	Exocytosis Protein transport Transport
Cellular component	Cell membrane Cytoplasm Membrane
Domain	Coiled coil
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	Golgi to plasma membrane transport Traceable author statement. Source: SGD bipolar cellular bud site selection Traceable author statement. Source: SGD protein transport Inferred from electronic annotation. Source: UniProtKB-KW vesicle docking involved in exocytosis Traceable author statement. Source: SGD vesicle fusion Traceable author statement. Source: SGD
Cellular component	exocyst Inferred from direct assay. Source: SGD mating projection tip Inferred from direct assay. Source: SGD plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	Rab GTPase binding Inferred from physical interaction. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
EXO84	P38261	3	EBI-16543,EBI-21567
SEC10	Q06245	3	EBI-16543,EBI-16504

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 910	910	Exocyst complex component SEC15		PRO_0000118959
Regions
	Coiled coil	88 – 116	29	Potential
Amino acid modifications
	Modified residue	286	1	Phosphoserine Ref.6

Sequences

	Sequence		Length	Mass (Da)
	P22224 [UniParc]. Last modified October 1, 1996. Version 2. Checksum: 818A5D27A1691D17	FASTA	910	105,064
	10 20 30 40 50 60 MDQEGQPLLS KDFQQVLLAT ASGNNSSWTE RAVLNNESTD AVKHEPALGQ NDVFDLDPLS 70 80 90 100 110 120 FDKWVPFLRR ALDKNQLDPV IDELENSIED NFQGLELQLL QDSQMNDKLE TSIDEIANIQ 130 140 150 160 170 180 GMVQDTLSSE ISKFQIRLSE SANELIVKKQ MYVNNKKISL KISEATILIT KVVRILELSS 190 200 210 220 230 240 KCQELITERK FFKVLQNLDS LEKLYLQEFK NYNFQFLIEI YNSIPFLQKV TKDECINLIR 250 260 270 280 290 300 NSLNLNLGKN LIKVGQEFVA IYENELLPQW LETRSKMKLT NFKFNSPIEI SMRDESFLAK 310 320 330 340 350 360 LNLGEFFQLD DFHDSIMIFQ NLNELSVLSG EFNKEYELRK TKLMYPLIWK KNKTAAYQMD 370 380 390 400 410 420 SLLRGTGTTP GSTAHDVSTD DPFTQSLSLH FLQDYFLKIL GFLLYDINLN KATEFILVDN 430 440 450 460 470 480 NYNSTNEFWD GLMDRLSPYL SYFIDEKLKT EEDMIKLKDF LCIYVAILEN FKLNIEPLYK 490 500 510 520 530 540 ILVSIFEKFC SVSLRAFDDE FQILLNDDDF MPLSINDKTL YEKVLKICWM KEGEHLSLPD 550 560 570 580 590 600 PTNGEPFAVT LPFSPLYPMT CTLAKKTYSK ITAFLSIFYR HELHTLNNIL VKTMDDIFND 610 620 630 640 650 660 IVNKKIRSKL ESTSREEIAQ ILVNLDYFII AAKEFSNFMT RENILQNPDM EIRLSSIKYL 670 680 690 700 710 720 AESRKLAETK LIELIDSKIS DILETIEIDW QITEVRQDPD ISIIDLAQFL EMMFASTLQN 730 740 750 760 770 780 LPYSVQTLLI FREFDSLTRQ FMGLLLHDTP STITHESIMN FEVDVNYLES IIPRIFPSTP 790 800 810 820 830 840 GTIDSNGYQS PMTPSTPTFP NANGVDAPTL FENNIKSLEA TFMELKQCIE LLKTQGKDYN 850 860 870 880 890 900 EPEIRLRKYS RIRQEDAALL LSKIQHFVSS VEGANGDDTS VMDSSSIFNS ESASVIDSNT 910 SRIAKFFNRR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The Sec15 protein responds to the function of the GTP binding protein, Sec4, to control vesicular traffic in yeast." Salminen A., Novick P.J. J. Cell Biol. 109:1023-1036(1989) [PubMed: 2504727] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K. Nature 387:81-84(1997) [PubMed: 9169869] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Sec8p and Sec15p are components of a plasma membrane-associated 19.5S particle that may function downstream of Sec4p to control exocytosis." Bowser R., Mueller H., Govindan B., Novick P. J. Cell Biol. 118:1041-1056(1992) [PubMed: 1512289] [Abstract] Cited for: FUNCTION.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-286, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z72755 Genomic DNA. Translation: CAA96951.1. BK006941 Genomic DNA. Translation: DAA07885.1.
PIR	S07838.
RefSeq	NP_011281.1. NM_001181099.1.
3D structure databases
ProteinModelPortal	P22224.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2681N.
IntAct	P22224. 24 interactions.
MINT	MINT-571081.
STRING	P22224.
Proteomic databases
PeptideAtlas	P22224.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YGL233W; YGL233W; YGL233W.
GeneID	852618.
KEGG	sce:YGL233W.
NMPDR	fig|4932.3.peg.2384.
Organism-specific databases
CYGD	YGL233w.
SGD	S000003202. SEC15.
Phylogenomic databases
eggNOG	fuNOG04382.
GeneTree	EFGT00050000003941.
HOGENOM	HBG204361.
OMA	KDECINL.
OrthoDB	EOG4SXRMQ.
Gene expression databases
ArrayExpress	P22224.
Genevestigator	P22224.
GermOnline	YGL233W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR007225. Sec15. [Graphical view]
PANTHER	PTHR12702. Sec15. 1 hit.
Pfam	PF04091. Sec15. 1 hit. [Graphical view]
PIRSF	PIRSF025007. Sec15. 1 hit.
ProtoNet	Search...
Other
NextBio	971826.

Entry information

Entry name

SEC15_YEAST

Accession

Primary (citable) accession number: P22224
Secondary accession number(s): D6VVA1

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1991
Last sequence update:	October 1, 1996
Last modified:	December 14, 2011
This is version 95 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program