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Protein

Serine/threonine-protein kinase RAD53

Gene

RAD53

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Controls S-phase checkpoint as well as G1 and G2 DNA damage checkpoints. Phosphorylates proteins on serine, threonine, and tyrosine. Prevents entry into anaphase and mitotic exit after DNA damage via regulation of the Polo kinase CDC5. Seems to be involved in the phosphorylation of RPH1.5 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei227ATPPROSITE-ProRule annotation1
Active sitei319Proton acceptor1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi204 – 212ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

  • deoxyribonucleoside triphosphate biosynthetic process Source: SGD
  • DNA damage checkpoint Source: SGD
  • DNA repair Source: SGD
  • DNA replication initiation Source: SGD
  • negative regulation of phosphorylation Source: SGD
  • nucleobase-containing compound metabolic process Source: SGD
  • protein localization Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase, Tyrosine-protein kinase

Keywords - Biological processi

Cell cycle, DNA damage

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-34050-MONOMER.
BRENDAi2.7.12.1. 984.
ReactomeiR-SCE-5693565. Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks.
R-SCE-69473. G2/M DNA damage checkpoint.
R-SCE-69601. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase RAD53 (EC:2.7.12.1)
Alternative name(s):
CHEK2 homolog
Serine-protein kinase 1
Gene namesi
Name:RAD53
Synonyms:MEC2, SAD1, SPK1
Ordered Locus Names:YPL153C
ORF Names:P2588
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPL153C.
SGDiS000006074. RAD53.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: SGD
  • nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000865951 – 821Serine/threonine-protein kinase RAD53Add BLAST821

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei24PhosphoserineCombined sources1
Modified residuei175PhosphoserineCombined sources1
Modified residuei547PhosphoserineCombined sources1
Modified residuei560PhosphoserineCombined sources1
Modified residuei774PhosphoserineCombined sources1
Modified residuei793PhosphoserineCombined sources1

Post-translational modificationi

Autophosphorylated.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP22216.
PRIDEiP22216.

PTM databases

iPTMnetiP22216.

Interactioni

Subunit structurei

Interacts with PIN4.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
ASF1P324478EBI-17843,EBI-3003
PIN4P342173EBI-17843,EBI-21256
RAD9P147378EBI-17843,EBI-14788
SGS1P351873EBI-17843,EBI-17059

Protein-protein interaction databases

BioGridi36030. 363 interactors.
DIPiDIP-2322N.
IntActiP22216. 14 interactors.
MINTiMINT-364105.

Structurei

Secondary structure

1821
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi16 – 24Combined sources9
Beta strandi31 – 43Combined sources13
Beta strandi46 – 50Combined sources5
Helixi52 – 57Combined sources6
Beta strandi59 – 61Combined sources3
Beta strandi64 – 71Combined sources8
Beta strandi74 – 77Combined sources4
Turni82 – 85Combined sources4
Beta strandi89 – 93Combined sources5
Beta strandi95 – 97Combined sources3
Beta strandi99 – 103Combined sources5
Beta strandi109 – 111Combined sources3
Beta strandi114 – 116Combined sources3
Beta strandi120 – 123Combined sources4
Beta strandi129 – 132Combined sources4
Helixi137 – 139Combined sources3
Beta strandi141 – 147Combined sources7
Helixi149 – 154Combined sources6
Beta strandi160 – 162Combined sources3
Helixi193 – 196Combined sources4
Beta strandi197 – 201Combined sources5
Beta strandi211 – 217Combined sources7
Turni218 – 220Combined sources3
Beta strandi223 – 229Combined sources7
Helixi241 – 248Combined sources8
Beta strandi259 – 264Combined sources6
Beta strandi269 – 273Combined sources5
Helixi281 – 288Combined sources8
Helixi293 – 312Combined sources20
Helixi322 – 324Combined sources3
Beta strandi325 – 329Combined sources5
Turni330 – 333Combined sources4
Beta strandi334 – 337Combined sources4
Helixi359 – 361Combined sources3
Helixi364 – 367Combined sources4
Turni384 – 386Combined sources3
Helixi387 – 403Combined sources17
Helixi413 – 421Combined sources9
Helixi428 – 432Combined sources5
Helixi437 – 446Combined sources10
Helixi451 – 453Combined sources3
Helixi457 – 461Combined sources5
Turni464 – 467Combined sources4
Helixi484 – 493Combined sources10
Beta strandi551 – 557Combined sources7
Helixi563 – 565Combined sources3
Beta strandi572 – 575Combined sources4
Beta strandi578 – 582Combined sources5
Beta strandi586 – 588Combined sources3
Beta strandi592 – 597Combined sources6
Beta strandi601 – 606Combined sources6
Beta strandi609 – 612Combined sources4
Beta strandi620 – 629Combined sources10
Beta strandi636 – 638Combined sources3
Beta strandi645 – 651Combined sources7
Beta strandi653 – 655Combined sources3
Beta strandi657 – 659Combined sources3
Beta strandi662 – 664Combined sources3
Beta strandi666 – 671Combined sources6
Beta strandi678 – 680Combined sources3
Turni684 – 687Combined sources4
Beta strandi688 – 691Combined sources4
Beta strandi692 – 695Combined sources4
Beta strandi703 – 707Combined sources5
Beta strandi710 – 712Combined sources3
Beta strandi716 – 718Combined sources3
Helixi721 – 728Combined sources8
Beta strandi804 – 806Combined sources3
Helixi811 – 813Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1DMZNMR-A573-730[»]
1FHQNMR-A573-730[»]
1FHRNMR-A573-730[»]
1G3GNMR-A1-164[»]
1G6GX-ray1.60A/B29-155[»]
1J4KNMR-A573-730[»]
1J4LNMR-A573-730[»]
1J4ONMR-A14-164[»]
1J4PNMR-A14-164[»]
1J4QNMR-A14-164[»]
1K2MNMR-A573-730[»]
1K2NNMR-A573-730[»]
1K3JNMR-A14-164[»]
1K3NNMR-A14-164[»]
1K3QNMR-A14-164[»]
1QU5NMR-A549-730[»]
2A0TNMR-A14-164[»]
2JQINMR-A14-164[»]
B3-12[»]
2JQLNMR-B3-12[»]
2YGVX-ray2.94E/F/G/H800-821[»]
4PDPX-ray2.59A/B170-512[»]
4PDSX-ray2.90A/B170-512[»]
5T2SX-ray2.40A/C22-162[»]
ProteinModelPortaliP22216.
SMRiP22216.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP22216.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini66 – 116FHA 1PROSITE-ProRule annotationAdd BLAST51
Domaini198 – 466Protein kinasePROSITE-ProRule annotationAdd BLAST269
Domaini601 – 664FHA 2PROSITE-ProRule annotationAdd BLAST64

Domaini

FHA domains are phosphothreonine recognition modules, FHA 1 strongly selects for Asp at position +3 relative to phosphothreonine, whereas FHA 2 selects for Ile in this position.

Sequence similaritiesi

Contains 2 FHA domains.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

GeneTreeiENSGT00800000124190.
HOGENOMiHOG000074515.
InParanoidiP22216.
KOiK02831.
OMAiHLPFNGK.
OrthoDBiEOG092C2FDZ.

Family and domain databases

Gene3Di2.60.200.20. 2 hits.
InterProiIPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase.
IPR000253. FHA_dom.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
IPR016256. Ser/Thr_kinase_Rad53.
IPR008984. SMAD_FHA_domain.
[Graphical view]
PANTHERiPTHR24347. PTHR24347. 1 hit.
PfamiPF00498. FHA. 2 hits.
PF00069. Pkinase. 1 hit.
[Graphical view]
PIRSFiPIRSF000661. Ser/Thr_PK_RAD53. 1 hit.
SMARTiSM00240. FHA. 2 hits.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF49879. SSF49879. 2 hits.
SSF56112. SSF56112. 1 hit.
PROSITEiPS50006. FHA_DOMAIN. 2 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P22216-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MENITQPTQQ STQATQRFLI EKFSQEQIGE NIVCRVICTT GQIPIRDLSA
60 70 80 90 100
DISQVLKEKR SIKKVWTFGR NPACDYHLGN ISRLSNKHFQ ILLGEDGNLL
110 120 130 140 150
LNDISTNGTW LNGQKVEKNS NQLLSQGDEI TVGVGVESDI LSLVIFINDK
160 170 180 190 200
FKQCLEQNKV DRIRSNLKNT SKIASPGLTS STASSMVANK TGIFKDFSII
210 220 230 240 250
DEVVGQGAFA TVKKAIERTT GKTFAVKIIS KRKVIGNMDG VTRELEVLQK
260 270 280 290 300
LNHPRIVRLK GFYEDTESYY MVMEFVSGGD LMDFVAAHGA VGEDAGREIS
310 320 330 340 350
RQILTAIKYI HSMGISHRDL KPDNILIEQD DPVLVKITDF GLAKVQGNGS
360 370 380 390 400
FMKTFCGTLA YVAPEVIRGK DTSVSPDEYE ERNEYSSLVD MWSMGCLVYV
410 420 430 440 450
ILTGHLPFSG STQDQLYKQI GRGSYHEGPL KDFRISEEAR DFIDSLLQVD
460 470 480 490 500
PNNRSTAAKA LNHPWIKMSP LGSQSYGDFS QISLSQSLSQ QKLLENMDDA
510 520 530 540 550
QYEFVKAQRK LQMEQQLQEQ DQEDQDGKIQ GFKIPAHAPI RYTQPKSIEA
560 570 580 590 600
ETREQKLLHS NNTENVKSSK KKGNGRFLTL KPLPDSIIQE SLEIQQGVNP
610 620 630 640 650
FFIGRSEDCN CKIEDNRLSR VHCFIFKKRH AVGKSMYESP AQGLDDIWYC
660 670 680 690 700
HTGTNVSYLN NNRMIQGTKF LLQDGDEIKI IWDKNNKFVI GFKVEINDTT
710 720 730 740 750
GLFNEGLGML QEQRVVLKQT AEEKDLVKKL TQMMAAQRAN QPSASSSSMS
760 770 780 790 800
AKKPPVSDTN NNGNNSVLND LVESPINANT GNILKRIHSV SLSQSQIDPS
810 820
KKVKRAKLDQ TSKGPENLQF S
Length:821
Mass (Da):91,962
Last modified:August 1, 1991 - v1
Checksum:i84A9612229CA72D1
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti204V → A in AAT93028 (PubMed:17322287).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M55623 Genomic DNA. Translation: AAA35070.1.
X96770 Genomic DNA. Translation: CAA65568.1.
Z73509 Genomic DNA. Translation: CAA97858.1.
AY693009 Genomic DNA. Translation: AAT93028.1.
BK006949 Genomic DNA. Translation: DAA11281.1.
PIRiA39616.
RefSeqiNP_015172.1. NM_001183967.1.

Genome annotation databases

EnsemblFungiiYPL153C; YPL153C; YPL153C.
GeneIDi855950.
KEGGisce:YPL153C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M55623 Genomic DNA. Translation: AAA35070.1.
X96770 Genomic DNA. Translation: CAA65568.1.
Z73509 Genomic DNA. Translation: CAA97858.1.
AY693009 Genomic DNA. Translation: AAT93028.1.
BK006949 Genomic DNA. Translation: DAA11281.1.
PIRiA39616.
RefSeqiNP_015172.1. NM_001183967.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1DMZNMR-A573-730[»]
1FHQNMR-A573-730[»]
1FHRNMR-A573-730[»]
1G3GNMR-A1-164[»]
1G6GX-ray1.60A/B29-155[»]
1J4KNMR-A573-730[»]
1J4LNMR-A573-730[»]
1J4ONMR-A14-164[»]
1J4PNMR-A14-164[»]
1J4QNMR-A14-164[»]
1K2MNMR-A573-730[»]
1K2NNMR-A573-730[»]
1K3JNMR-A14-164[»]
1K3NNMR-A14-164[»]
1K3QNMR-A14-164[»]
1QU5NMR-A549-730[»]
2A0TNMR-A14-164[»]
2JQINMR-A14-164[»]
B3-12[»]
2JQLNMR-B3-12[»]
2YGVX-ray2.94E/F/G/H800-821[»]
4PDPX-ray2.59A/B170-512[»]
4PDSX-ray2.90A/B170-512[»]
5T2SX-ray2.40A/C22-162[»]
ProteinModelPortaliP22216.
SMRiP22216.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36030. 363 interactors.
DIPiDIP-2322N.
IntActiP22216. 14 interactors.
MINTiMINT-364105.

PTM databases

iPTMnetiP22216.

Proteomic databases

MaxQBiP22216.
PRIDEiP22216.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYPL153C; YPL153C; YPL153C.
GeneIDi855950.
KEGGisce:YPL153C.

Organism-specific databases

EuPathDBiFungiDB:YPL153C.
SGDiS000006074. RAD53.

Phylogenomic databases

GeneTreeiENSGT00800000124190.
HOGENOMiHOG000074515.
InParanoidiP22216.
KOiK02831.
OMAiHLPFNGK.
OrthoDBiEOG092C2FDZ.

Enzyme and pathway databases

BioCyciYEAST:G3O-34050-MONOMER.
BRENDAi2.7.12.1. 984.
ReactomeiR-SCE-5693565. Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks.
R-SCE-69473. G2/M DNA damage checkpoint.
R-SCE-69601. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.

Miscellaneous databases

EvolutionaryTraceiP22216.
PROiP22216.

Family and domain databases

Gene3Di2.60.200.20. 2 hits.
InterProiIPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase.
IPR000253. FHA_dom.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
IPR016256. Ser/Thr_kinase_Rad53.
IPR008984. SMAD_FHA_domain.
[Graphical view]
PANTHERiPTHR24347. PTHR24347. 1 hit.
PfamiPF00498. FHA. 2 hits.
PF00069. Pkinase. 1 hit.
[Graphical view]
PIRSFiPIRSF000661. Ser/Thr_PK_RAD53. 1 hit.
SMARTiSM00240. FHA. 2 hits.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF49879. SSF49879. 2 hits.
SSF56112. SSF56112. 1 hit.
PROSITEiPS50006. FHA_DOMAIN. 2 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRAD53_YEAST
AccessioniPrimary (citable) accession number: P22216
Secondary accession number(s): D6W3L5, Q6B1S1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: August 1, 1991
Last modified: November 30, 2016
This is version 189 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 6900 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.