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Protein

Protein transport protein SEC22

Gene

SEC22

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Nonessential SNARE involved in targeting and fusion of ER-derived transport vesicles with the Golgi complex as well as Golgi-derived retrograde transport vesicles with the ER.4 Publications

GO - Molecular functioni

  • SNAP receptor activity Source: SGD

GO - Biological processi

  • ER to Golgi vesicle-mediated transport Source: SGD
  • protein transport Source: UniProtKB-KW
  • retrograde vesicle-mediated transport, Golgi to ER Source: SGD
  • vesicle fusion Source: SGD
  • vesicle fusion with endoplasmic reticulum Source: SGD
  • vesicle fusion with Golgi apparatus Source: SGD
Complete GO annotation...

Keywords - Biological processi

ER-Golgi transport, Protein transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-32368-MONOMER.
ReactomeiR-SCE-204005. COPII (Coat Protein 2) Mediated Vesicle Transport.
R-SCE-5694530. Cargo concentration in the ER.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein transport protein SEC22
Alternative name(s):
Suppressor of loss of YPT1 protein 2
Gene namesi
Name:SEC22
Synonyms:SLY2, TSL26
Ordered Locus Names:YLR268W
ORF Names:L8479.3
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XII

Organism-specific databases

EuPathDBiFungiDB:YLR268W.
SGDiS000004258. SEC22.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 192192CytoplasmicSequence analysisAdd
BLAST
Transmembranei193 – 21321Helical; Anchor for type IV membrane proteinSequence analysisAdd
BLAST
Topological domaini214 – 2141VesicularSequence analysis

GO - Cellular componenti

  • endoplasmic reticulum Source: SGD
  • endoplasmic reticulum membrane Source: UniProtKB-SubCell
  • ER to Golgi transport vesicle membrane Source: SGD
  • Golgi apparatus Source: SGD
  • Golgi membrane Source: UniProtKB-SubCell
  • integral component of membrane Source: SGD
  • SNARE complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 214214Protein transport protein SEC22PRO_0000206769Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei160 – 1601PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP22214.
PeptideAtlasiP22214.
TopDownProteomicsiP22214.

PTM databases

iPTMnetiP22214.

Interactioni

Subunit structurei

Component of two distinct SNARE complexes consisting of SED5, BOS1, BET1 and SEC22 or UFE1, USE1, SEC20 and SEC22. YKT6 can probably replace SEC22 as subunit of either complex. Interacts with SEC24, YIF1 and YIP1.5 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
SED5Q015903EBI-16577,EBI-16930

GO - Molecular functioni

  • SNAP receptor activity Source: SGD

Protein-protein interaction databases

BioGridi31536. 482 interactions.
DIPiDIP-2234N.
IntActiP22214. 16 interactions.
MINTiMINT-480282.

Structurei

3D structure databases

ProteinModelPortaliP22214.
SMRiP22214. Positions 1-186.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini6 – 117112LonginPROSITE-ProRule annotationAdd
BLAST
Domaini132 – 19261v-SNARE coiled-coil homologyPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the synaptobrevin family.Curated
Contains 1 longin domain.PROSITE-ProRule annotation
Contains 1 v-SNARE coiled-coil homology domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00510000046833.
HOGENOMiHOG000044957.
InParanoidiP22214.
KOiK08517.
OMAiQSANEAT.
OrthoDBiEOG7W9S5V.

Family and domain databases

Gene3Di1.10.3840.10. 1 hit.
3.30.450.50. 1 hit.
InterProiIPR011012. Longin-like_dom.
IPR010908. Longin_dom.
IPR001388. Synaptobrevin.
[Graphical view]
PfamiPF13774. Longin. 1 hit.
PF00957. Synaptobrevin. 1 hit.
[Graphical view]
SMARTiSM01270. Longin. 1 hit.
[Graphical view]
SUPFAMiSSF64356. SSF64356. 1 hit.
PROSITEiPS50859. LONGIN. 1 hit.
PS50892. V_SNARE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P22214-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIKSTLIYRE DGLPLCTSVD NENDPSLFEQ KQKVKIVVSR LTPQSATEAT
60 70 80 90 100
LESGSFEIHY LKKSMVYYFV ICESGYPRNL AFSYLNDIAQ EFEHSFANEY
110 120 130 140 150
PKPTVRPYQF VNFDNFLQMT KKSYSDKKVQ DNLDQLNQEL VGVKQIMSKN
160 170 180 190 200
IEDLLYRGDS LDKMSDMSSS LKETSKRYRK SAQKINFDLL ISQYAPIVIV
210
AFFFVFLFWW IFLK
Length:214
Mass (Da):25,058
Last modified:August 1, 1991 - v1
Checksum:i9FA3E34EAAFB9536
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X54236 Genomic DNA. Translation: CAA38142.1.
U17244 Genomic DNA. Translation: AAB67373.1.
AY557935 Genomic DNA. Translation: AAS56261.1.
BK006945 Genomic DNA. Translation: DAA09582.1.
PIRiS51405.
RefSeqiNP_013370.1. NM_001182155.1.

Genome annotation databases

EnsemblFungiiYLR268W; YLR268W; YLR268W.
GeneIDi850973.
KEGGisce:YLR268W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X54236 Genomic DNA. Translation: CAA38142.1.
U17244 Genomic DNA. Translation: AAB67373.1.
AY557935 Genomic DNA. Translation: AAS56261.1.
BK006945 Genomic DNA. Translation: DAA09582.1.
PIRiS51405.
RefSeqiNP_013370.1. NM_001182155.1.

3D structure databases

ProteinModelPortaliP22214.
SMRiP22214. Positions 1-186.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31536. 482 interactions.
DIPiDIP-2234N.
IntActiP22214. 16 interactions.
MINTiMINT-480282.

PTM databases

iPTMnetiP22214.

Proteomic databases

MaxQBiP22214.
PeptideAtlasiP22214.
TopDownProteomicsiP22214.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYLR268W; YLR268W; YLR268W.
GeneIDi850973.
KEGGisce:YLR268W.

Organism-specific databases

EuPathDBiFungiDB:YLR268W.
SGDiS000004258. SEC22.

Phylogenomic databases

GeneTreeiENSGT00510000046833.
HOGENOMiHOG000044957.
InParanoidiP22214.
KOiK08517.
OMAiQSANEAT.
OrthoDBiEOG7W9S5V.

Enzyme and pathway databases

BioCyciYEAST:G3O-32368-MONOMER.
ReactomeiR-SCE-204005. COPII (Coat Protein 2) Mediated Vesicle Transport.
R-SCE-5694530. Cargo concentration in the ER.

Miscellaneous databases

NextBioi967475.
PROiP22214.

Family and domain databases

Gene3Di1.10.3840.10. 1 hit.
3.30.450.50. 1 hit.
InterProiIPR011012. Longin-like_dom.
IPR010908. Longin_dom.
IPR001388. Synaptobrevin.
[Graphical view]
PfamiPF13774. Longin. 1 hit.
PF00957. Synaptobrevin. 1 hit.
[Graphical view]
SMARTiSM01270. Longin. 1 hit.
[Graphical view]
SUPFAMiSSF64356. SSF64356. 1 hit.
PROSITEiPS50859. LONGIN. 1 hit.
PS50892. V_SNARE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation, DNA sequence and regulation of a new cell division cycle gene from the yeast Saccharomyces cerevisiae."
    Hasegawa H., Sakai A., Sugino A.
    Yeast 5:509-524(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Identification and structure of four yeast genes (SLY) that are able to suppress the functional loss of YPT1, a member of the RAS superfamily."
    Dascher C., Ossig R., Gallwitz D., Schmitt H.D.
    Mol. Cell. Biol. 11:872-885(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
  3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
    Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H.
    , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
    Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  6. "Topological restriction of SNARE-dependent membrane fusion."
    Parlati F., McNew J.A., Fukuda R., Miller R., Sollner T.H., Rothman J.E.
    Nature 407:194-198(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH BET1; BOS1 AND SED5.
  7. Cited for: INTERACTION WITH SEC24.
  8. "Use1p is a yeast SNARE protein required for retrograde traffic to the ER."
    Dilcher M., Veith B., Chidambaram S., Hartmann E., Schmitt H.D., Fischer von Mollard G.
    EMBO J. 22:3664-3674(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH SEC20; UFE1 AND USE1.
  9. "The Yip1p.Yif1p complex is required for the fusion competence of endoplasmic reticulum-derived vesicles."
    Barrowman J., Wang W., Zhang Y., Ferro-Novick S.
    J. Biol. Chem. 278:19878-19884(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH YIF1 AND YIP1.
  10. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  11. Cited for: FUNCTION, INTERACTION WITH UFE1 AND USE1.
  12. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-160, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  13. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiSEC22_YEAST
AccessioniPrimary (citable) accession number: P22214
Secondary accession number(s): D6VYR6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: August 1, 1991
Last modified: May 11, 2016
This is version 163 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 396 molecules/cell in log phase SD medium.1 Publication
Deletion of SEC22 is viable. Overexpression of SEC22 suppresses deletion of YPT1.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.