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Protein

Protein transport protein SEC22

Gene

SEC22

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Nonessential SNARE involved in targeting and fusion of ER-derived transport vesicles with the Golgi complex as well as Golgi-derived retrograde transport vesicles with the ER.4 Publications

Miscellaneous

Present with 396 molecules/cell in log phase SD medium.1 Publication
Deletion of SEC22 is viable. Overexpression of SEC22 suppresses deletion of YPT1.

GO - Molecular functioni

  • SNAP receptor activity Source: SGD
  • SNARE binding Source: GO_Central

GO - Biological processi

  • ER to Golgi vesicle-mediated transport Source: SGD
  • protein transport Source: UniProtKB-KW
  • retrograde vesicle-mediated transport, Golgi to ER Source: SGD
  • vesicle fusion Source: SGD
  • vesicle fusion with endoplasmic reticulum Source: SGD
  • vesicle fusion with Golgi apparatus Source: SGD

Keywordsi

Biological processER-Golgi transport, Protein transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-32368-MONOMER.
ReactomeiR-SCE-204005. COPII (Coat Protein 2) Mediated Vesicle Transport.
R-SCE-5694530. Cargo concentration in the ER.
R-SCE-6811434. COPI-dependent Golgi-to-ER retrograde traffic.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein transport protein SEC22
Alternative name(s):
Suppressor of loss of YPT1 protein 2
Gene namesi
Name:SEC22
Synonyms:SLY2, TSL26
Ordered Locus Names:YLR268W
ORF Names:L8479.3
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XII

Organism-specific databases

EuPathDBiFungiDB:YLR268W.
SGDiS000004258. SEC22.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 192CytoplasmicSequence analysisAdd BLAST192
Transmembranei193 – 213Helical; Anchor for type IV membrane proteinSequence analysisAdd BLAST21
Topological domaini214VesicularSequence analysis1

Keywords - Cellular componenti

Endoplasmic reticulum, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002067691 – 214Protein transport protein SEC22Add BLAST214

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei160PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP22214.
PRIDEiP22214.
TopDownProteomicsiP22214.

PTM databases

iPTMnetiP22214.

Interactioni

Subunit structurei

Component of two distinct SNARE complexes consisting of SED5, BOS1, BET1 and SEC22 or UFE1, USE1, SEC20 and SEC22. YKT6 can probably replace SEC22 as subunit of either complex. Interacts with SEC24, YIF1 and YIP1.5 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
SED5Q015903EBI-16577,EBI-16930

GO - Molecular functioni

  • SNAP receptor activity Source: SGD
  • SNARE binding Source: GO_Central

Protein-protein interaction databases

BioGridi31536. 1226 interactors.
DIPiDIP-2234N.
IntActiP22214. 19 interactors.
MINTiMINT-480282.
STRINGi4932.YLR268W.

Structurei

3D structure databases

ProteinModelPortaliP22214.
SMRiP22214.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini6 – 117LonginPROSITE-ProRule annotationAdd BLAST112
Domaini132 – 192v-SNARE coiled-coil homologyPROSITE-ProRule annotationAdd BLAST61

Sequence similaritiesi

Belongs to the synaptobrevin family.Curated

Keywords - Domaini

Coiled coil, Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00510000046833.
HOGENOMiHOG000044957.
InParanoidiP22214.
KOiK08517.
OMAiMLCASVD.
OrthoDBiEOG092C4MCV.

Family and domain databases

InterProiView protein in InterPro
IPR011012. Longin-like_dom.
IPR010908. Longin_dom.
IPR001388. Synaptobrevin.
PfamiView protein in Pfam
PF13774. Longin. 1 hit.
PF00957. Synaptobrevin. 1 hit.
SMARTiView protein in SMART
SM01270. Longin. 1 hit.
SUPFAMiSSF64356. SSF64356. 1 hit.
PROSITEiView protein in PROSITE
PS50859. LONGIN. 1 hit.
PS50892. V_SNARE. 1 hit.

Sequencei

Sequence statusi: Complete.

P22214-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIKSTLIYRE DGLPLCTSVD NENDPSLFEQ KQKVKIVVSR LTPQSATEAT
60 70 80 90 100
LESGSFEIHY LKKSMVYYFV ICESGYPRNL AFSYLNDIAQ EFEHSFANEY
110 120 130 140 150
PKPTVRPYQF VNFDNFLQMT KKSYSDKKVQ DNLDQLNQEL VGVKQIMSKN
160 170 180 190 200
IEDLLYRGDS LDKMSDMSSS LKETSKRYRK SAQKINFDLL ISQYAPIVIV
210
AFFFVFLFWW IFLK
Length:214
Mass (Da):25,058
Last modified:August 1, 1991 - v1
Checksum:i9FA3E34EAAFB9536
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X54236 Genomic DNA. Translation: CAA38142.1.
U17244 Genomic DNA. Translation: AAB67373.1.
AY557935 Genomic DNA. Translation: AAS56261.1.
BK006945 Genomic DNA. Translation: DAA09582.1.
PIRiS51405.
RefSeqiNP_013370.1. NM_001182155.1.

Genome annotation databases

EnsemblFungiiYLR268W; YLR268W; YLR268W.
GeneIDi850973.
KEGGisce:YLR268W.

Similar proteinsi

Entry informationi

Entry nameiSEC22_YEAST
AccessioniPrimary (citable) accession number: P22214
Secondary accession number(s): D6VYR6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: August 1, 1991
Last modified: September 27, 2017
This is version 175 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names