P22209 (KIN3_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Serine/threonine-protein kinase KIN3
EC=2.7.11.1

Gene names

Name:	KIN3
Synonyms:	NPK1
Ordered Locus Names:	YAR018C
ORF Names:	FUN52

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	435 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This protein is probably a serine/threonine protein kinase.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Sequence similarities	Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Contains 1 protein kinase domain.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	chromosome segregation Inferred from mutant phenotype PubMed 12386167. Source: SGD
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein kinase activity Inferred from sequence or structural similarity Ref.6. Source: SGD protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 435	435	Serine/threonine-protein kinase KIN3		PRO_0000086134
Regions
	Domain	25 – 343	319	Protein kinase
	Nucleotide binding	31 – 39	9	ATP By similarity
Sites
	Active site	174	1	Proton acceptor By similarity
	Binding site	54	1	ATP By similarity
Experimental info
	Sequence conflict	92	1	F → L in AAB04029. Ref.6
	Sequence conflict	98	1	V → L in AAB04029. Ref.6
	Sequence conflict	121	1	E → Q in AAB04029. Ref.6
	Sequence conflict	130	1	V → I in AAB04029. Ref.6
	Sequence conflict	235 – 238	4	AKSL → SQIS in AAB04029. Ref.6
	Sequence conflict	358	1	K → R in AAB04029. Ref.6

Sequences

	Sequence		Length	Mass (Da)
	P22209 [UniParc]. Last modified August 1, 1992. Version 2. Checksum: 7CB49CD6A93CA019	FASTA	435	51,203
	10 20 30 40 50 60 MHRRQFFQEY RSPQQQQGHP PRSEYQVLEE IGRGSFGSVR KVIHIPTKKL LVRKDIKYGH 70 80 90 100 110 120 MNSKERQQLI AECSILSQLK HENIVEFYNW DFDEQKEVLY LYMEYCSRGD LSQMIKHYKQ 130 140 150 160 170 180 EHKYIPEKIV WGILAQLLTA LYKCHYGVEL PTLTTIYDRM KPPVKGKNIV IHRDLKPGNI 190 200 210 220 230 240 FLSYDDSDYN INEQVDGHEE VNSNYYRDHR VNSGKRGSPM DYSQVVVKLG DFGLAKSLET 250 260 270 280 290 300 SIQFATTYVG TPYYMSPEVL MDQPYSPLSD IWSLGCVIFE MCSLHPPFQA KNYLELQTKI 310 320 330 340 350 360 KNGKCDTVPE YYSRGLNAII HSMIDVNLRT RPSTFELLQD IQIRTARKSL QLERFERKLL 370 380 390 400 410 420 DYENELTNIE KILEKQAIEY ERELSQLKEQ FTQAVEERAR EVISGKKVGK VPESINGYYG 430 KKFAKPAYHW QTRYR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning of chromosome I DNA from Saccharomyces cerevisiae: analysis of the FUN52 gene, whose product has homology to protein kinases." Barton A.B., Davies C.J., Hutchison C.A. III, Kaback D.B. Gene 117:137-140(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"NPK1, a nonessential protein kinase gene in Saccharomyces cerevisiae with similarity to Aspergillus nidulans nimA." Schweitzer B., Philippsen P. Mol. Gen. Genet. 234:164-167(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Sequencing of chromosome I of Saccharomyces cerevisiae: analysis of the 42 kbp SPO7-CENI-CDC15 region." Clark M.W., Keng T., Storms R.K., Zhong W.-W., Fortin N., Zeng B., Delaney S., Ouellette B.F.F., Barton A.B., Kaback D.B., Bussey H. Yeast 10:535-541(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[4]	"The nucleotide sequence of chromosome I from Saccharomyces cerevisiae." Bussey H., Kaback D.B., Zhong W.-W., Vo D.H., Clark M.W., Fortin N., Hall J., Ouellette B.F.F., Keng T., Barton A.B., Su Y., Davies C.J., Storms R.K. Proc. Natl. Acad. Sci. U.S.A. 92:3809-3813(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	"The reference genome sequence of Saccharomyces cerevisiae: Then and now." Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M. G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[6]	"Isolation of a novel protein kinase-encoding gene from yeast by oligodeoxyribonucleotide probing." Jones D.G.L., Rosamond J. Gene 90:87-92(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-369. Strain: SB303.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M67445 Genomic DNA. Translation: AAA34399.2. X60549 Genomic DNA. Translation: CAA43042.1. L22015 Genomic DNA. Translation: AAC04964.1. M55416 Genomic DNA. Translation: AAB04029.1. BK006935 Genomic DNA. Translation: DAA06995.1.
PIR	S23580.
RefSeq	NP_009410.1. NM_001178217.1.
3D structure databases
ProteinModelPortal	P22209.
SMR	P22209. Positions 1-405.
ModBase	Search...
MobiDB	Search...
Protein-protein interaction databases
BioGrid	31800. 190 interactions.
DIP	DIP-1646N.
IntAct	P22209. 22 interactions.
MINT	MINT-397142.
STRING	4932.YAR018C.
Proteomic databases
MaxQB	P22209.
PaxDb	P22209.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YAR018C; YAR018C; YAR018C.
GeneID	851273.
KEGG	sce:YAR018C.
Organism-specific databases
CYGD	YAR018c.
SGD	S000000071. KIN3.
Phylogenomic databases
eggNOG	COG0515.
GeneTree	ENSGT00720000108402.
HOGENOM	HOG000233029.
KO	K08857.
OMA	EFATTYV.
OrthoDB	EOG7VB2QQ.
Enzyme and pathway databases
BioCyc	YEAST:G3O-28874-MONOMER.
BRENDA	2.7.11.1. 984.
Gene expression databases
Genevestigator	P22209.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_dom. IPR017441. Protein_kinase_ATP_BS. IPR008271. Ser/Thr_kinase_AS. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SUPFAM	SSF56112. SSF56112. 2 hits.
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	968254.
PRO	P22209.

Entry information

Entry name

KIN3_YEAST

Accession

Primary (citable) accession number: P22209
Secondary accession number(s): D6VPM5

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1991
Last sequence update:	August 1, 1992
Last modified:	June 11, 2014
This is version 128 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program