Heat shock protein SSA4 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P22202 (HSP74_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 29, 2013. Version 125. History...

Clusters with 100%, 90%, 50% identity |

Documents (4) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Heat shock protein SSA4

Gene names

Name:	SSA4
Ordered Locus Names:	YER103W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	642 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Subcellular location	Cytoplasm.
Induction	HSP SSA4 expression is restricted to conditions of stress.
Miscellaneous	Present with 17900 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the heat shock protein 70 family.

Ontologies

Keywords
Biological process	Stress response
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
PTM	Acetylation Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	SRP-dependent cotranslational protein targeting to membrane, translocation Inferred from mutant phenotype PubMed 8754838. Source: SGD protein folding Inferred from genetic interaction PubMed 9789005. Source: SGD response to stress Inferred from direct assay Ref.1. Source: SGD
Cellular_component	cytoplasm Inferred from direct assay PubMed 11279056. Source: SGD nucleus Inferred from direct assay PubMed 11279056. Source: SGD
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATPase activity Inferred from sequence or structural similarity PubMed 8151709. Source: SGD unfolded protein binding Inferred from genetic interaction PubMed 9789005. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

Removed By similarity

Chain

2 – 642

641

Heat shock protein SSA4

PRO_0000078388

Amino acid modifications

Modified residue

N-acetylserine By similarity

Modified residue

423

Phosphoserine Ref.5

Modified residue

493

Phosphothreonine Ref.5

Modified residue

552

Phosphoserine Ref.5

Secondary structure

642

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P22202 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 7A976357AC35C352
Show »

FASTA

642

69,651

        10         20         30         40         50         60 
MSKAVGIDLG TTYSCVAHFA NDRVEIIAND QGNRTTPSYV AFTDTERLIG DAAKNQAAMN 

        70         80         90        100        110        120 
PHNTVFDAKR LIGRKFDDPE VTNDAKHYPF KVIDKGGKPV VQVEYKGETK TFTPEEISSM 

       130        140        150        160        170        180 
ILTKMKETAE NFLGTEVKDA VVTVPAYFND SQRQATKDAG TIAGLNVLRI INEPTAAAIA 

       190        200        210        220        230        240 
YGLDKKSQKE HNVLIFDLGG GTFDVSLLSI DEGVFEVKAT AGDTHLGGED FDSRLVNFLA 

       250        260        270        280        290        300 
EEFKRKNKKD LTTNQRSLRR LRTAAERAKR TLSSSAQTSI EIDSLFEGID FYTSITRARF 

       310        320        330        340        350        360 
EELCADLFRS TLEPVEKVLA DSKLDKSQID EIVLVGGSTR IPKVQKLVSD FFNGKEPNRS 

       370        380        390        400        410        420 
INPDEAVAYG AAVQAAILTG DQSSTTQDLL LLDVAPLSLG IETAGGIMTK LIPRNSTIPT 

       430        440        450        460        470        480 
KKSEVFSTYA DNQPGVLIQV FEGERTRTKD NNLLGKFELS GIPPAPRGVP QIEVTFDIDA 

       490        500        510        520        530        540 
NGILNVSAVE KGTGKSNKIT ITNDKGRLSK EDIDKMVAEA EKFKAEDEQE AQRVQAKNQL 

       550        560        570        580        590        600 
ESYAFTLKNS VSENNFKEKV GEEDARKLEA AAQDAINWLD ASQAASTEEY KERQKELEGV 

       610        620        630        640 
ANPIMSKFYG AAGGAPGAGP VPGAGAGPTG APDNGPTVEE VD

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structure and regulation of the SSA4 HSP70 gene of Saccharomyces cerevisiae." Boorstein W.R., Craig E.A. J. Biol. Chem. 265:18912-18921(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: DS10.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome V." Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W. Davis R.W. Nature 387:78-81(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-423; THR-493 AND SER-552, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

J05637 Genomic DNA. Translation: AAA63574.1.
U18839 Genomic DNA. Translation: AAB64658.1.
BK006939 Genomic DNA. Translation: DAA07764.1.

PIR

B36590.

RefSeq

NP_011029.3. NM_001178994.3.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3UPV	X-ray	1.60	B	636-642	[»]

ProteinModelPortal

P22202.

SMR

P22202. Positions 1-605.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-3916N.

IntAct

P22202. 13 interactions.

MINT

MINT-523139.

STRING

4932.YER103W.

Proteomic databases

PaxDb

P22202.

PeptideAtlas

P22202.

PRIDE

P22202.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YER103W; YER103W; YER103W.

GeneID

856840.

KEGG

sce:YER103W.

Organism-specific databases

SGD

S000000905. SSA4.

Phylogenomic databases

eggNOG

COG0443.

GeneTree

ENSGT00700000104788.

HOGENOM

HOG000228135.

K03283.

OMA

DVANPIM.

OrthoDB

EOG476P7G.

Enzyme and pathway databases

BioCyc

YEAST:G3O-30268-MONOMER.

Gene expression databases

Genevestigator

P22202.

GermOnline

YER103W. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR018181. Heat_shock_70_CS.
IPR013126. Hsp_70_fam.
[Graphical view]

Pfam

PF00012. HSP70. 1 hit.
[Graphical view]

PRINTS

PR00301. HEATSHOCK70.

PROSITE

PS00297. HSP70_1. 1 hit.
PS00329. HSP70_2. 1 hit.
PS01036. HSP70_3. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

983153.

Entry information

Entry name

HSP74_YEAST

Accession

Primary (citable) accession number: P22202
Secondary accession number(s): D3DM10

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1991
Last sequence update:	January 23, 2007
Last modified:	May 29, 2013
This is version 125 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome V

Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Amino acid modifications

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents