Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Heat shock protein SSA4

Gene

SSA4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

GO - Molecular functioni

  1. ATPase activity Source: SGD
  2. ATP binding Source: UniProtKB-KW
  3. unfolded protein binding Source: SGD

GO - Biological processi

  1. cellular response to heat Source: SGD
  2. protein folding Source: SGD
  3. SRP-dependent cotranslational protein targeting to membrane, translocation Source: SGD
Complete GO annotation...

Keywords - Biological processi

Stress response

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-30268-MONOMER.
ReactomeiREACT_205607. Regulation of HSF1-mediated heat shock response.
REACT_219346. HSF1-dependent transactivation.
REACT_271186. Attenuation phase.

Names & Taxonomyi

Protein namesi
Recommended name:
Heat shock protein SSA4
Gene namesi
Name:SSA4
Ordered Locus Names:YER103W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome V

Organism-specific databases

SGDiS000000905. SSA4.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: SGD
  2. nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 642642Heat shock protein SSA4PRO_0000078388Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei552 – 5521Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP22202.
PaxDbiP22202.
PeptideAtlasiP22202.
PRIDEiP22202.

Expressioni

Inductioni

HSP SSA4 expression is restricted to conditions of stress.

Gene expression databases

GenevestigatoriP22202.

Interactioni

Protein-protein interaction databases

BioGridi36849. 41 interactions.
DIPiDIP-3916N.
IntActiP22202. 19 interactions.
MINTiMINT-523139.
STRINGi4932.YER103W.

Structurei

Secondary structure

1
642
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi637 – 6393Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3UPVX-ray1.60B636-642[»]
ProteinModelPortaliP22202.
SMRiP22202. Positions 1-609.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the heat shock protein 70 family.Curated

Phylogenomic databases

eggNOGiCOG0443.
GeneTreeiENSGT00780000122064.
HOGENOMiHOG000228135.
InParanoidiP22202.
KOiK03283.
OMAiMRNTIRE.
OrthoDBiEOG728916.

Family and domain databases

Gene3Di1.20.1270.10. 1 hit.
2.60.34.10. 1 hit.
InterProiIPR018181. Heat_shock_70_CS.
IPR029048. HSP70_C.
IPR029047. HSP70_peptide-bd.
IPR013126. Hsp_70_fam.
[Graphical view]
PfamiPF00012. HSP70. 1 hit.
[Graphical view]
PRINTSiPR00301. HEATSHOCK70.
SUPFAMiSSF100920. SSF100920. 1 hit.
SSF100934. SSF100934. 1 hit.
PROSITEiPS00297. HSP70_1. 1 hit.
PS00329. HSP70_2. 1 hit.
PS01036. HSP70_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P22202-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKAVGIDLG TTYSCVAHFA NDRVEIIAND QGNRTTPSYV AFTDTERLIG
60 70 80 90 100
DAAKNQAAMN PHNTVFDAKR LIGRKFDDPE VTNDAKHYPF KVIDKGGKPV
110 120 130 140 150
VQVEYKGETK TFTPEEISSM ILTKMKETAE NFLGTEVKDA VVTVPAYFND
160 170 180 190 200
SQRQATKDAG TIAGLNVLRI INEPTAAAIA YGLDKKSQKE HNVLIFDLGG
210 220 230 240 250
GTFDVSLLSI DEGVFEVKAT AGDTHLGGED FDSRLVNFLA EEFKRKNKKD
260 270 280 290 300
LTTNQRSLRR LRTAAERAKR TLSSSAQTSI EIDSLFEGID FYTSITRARF
310 320 330 340 350
EELCADLFRS TLEPVEKVLA DSKLDKSQID EIVLVGGSTR IPKVQKLVSD
360 370 380 390 400
FFNGKEPNRS INPDEAVAYG AAVQAAILTG DQSSTTQDLL LLDVAPLSLG
410 420 430 440 450
IETAGGIMTK LIPRNSTIPT KKSEVFSTYA DNQPGVLIQV FEGERTRTKD
460 470 480 490 500
NNLLGKFELS GIPPAPRGVP QIEVTFDIDA NGILNVSAVE KGTGKSNKIT
510 520 530 540 550
ITNDKGRLSK EDIDKMVAEA EKFKAEDEQE AQRVQAKNQL ESYAFTLKNS
560 570 580 590 600
VSENNFKEKV GEEDARKLEA AAQDAINWLD ASQAASTEEY KERQKELEGV
610 620 630 640
ANPIMSKFYG AAGGAPGAGP VPGAGAGPTG APDNGPTVEE VD
Length:642
Mass (Da):69,651
Last modified:January 23, 2007 - v3
Checksum:i7A976357AC35C352
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J05637 Genomic DNA. Translation: AAA63574.1.
U18839 Genomic DNA. Translation: AAB64658.1.
BK006939 Genomic DNA. Translation: DAA07764.1.
PIRiB36590.
RefSeqiNP_011029.3. NM_001178994.3.

Genome annotation databases

EnsemblFungiiYER103W; YER103W; YER103W.
GeneIDi856840.
KEGGisce:YER103W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J05637 Genomic DNA. Translation: AAA63574.1.
U18839 Genomic DNA. Translation: AAB64658.1.
BK006939 Genomic DNA. Translation: DAA07764.1.
PIRiB36590.
RefSeqiNP_011029.3. NM_001178994.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3UPVX-ray1.60B636-642[»]
ProteinModelPortaliP22202.
SMRiP22202. Positions 1-609.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36849. 41 interactions.
DIPiDIP-3916N.
IntActiP22202. 19 interactions.
MINTiMINT-523139.
STRINGi4932.YER103W.

Proteomic databases

MaxQBiP22202.
PaxDbiP22202.
PeptideAtlasiP22202.
PRIDEiP22202.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYER103W; YER103W; YER103W.
GeneIDi856840.
KEGGisce:YER103W.

Organism-specific databases

SGDiS000000905. SSA4.

Phylogenomic databases

eggNOGiCOG0443.
GeneTreeiENSGT00780000122064.
HOGENOMiHOG000228135.
InParanoidiP22202.
KOiK03283.
OMAiMRNTIRE.
OrthoDBiEOG728916.

Enzyme and pathway databases

BioCyciYEAST:G3O-30268-MONOMER.
ReactomeiREACT_205607. Regulation of HSF1-mediated heat shock response.
REACT_219346. HSF1-dependent transactivation.
REACT_271186. Attenuation phase.

Miscellaneous databases

NextBioi983153.

Gene expression databases

GenevestigatoriP22202.

Family and domain databases

Gene3Di1.20.1270.10. 1 hit.
2.60.34.10. 1 hit.
InterProiIPR018181. Heat_shock_70_CS.
IPR029048. HSP70_C.
IPR029047. HSP70_peptide-bd.
IPR013126. Hsp_70_fam.
[Graphical view]
PfamiPF00012. HSP70. 1 hit.
[Graphical view]
PRINTSiPR00301. HEATSHOCK70.
SUPFAMiSSF100920. SSF100920. 1 hit.
SSF100934. SSF100934. 1 hit.
PROSITEiPS00297. HSP70_1. 1 hit.
PS00329. HSP70_2. 1 hit.
PS01036. HSP70_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Structure and regulation of the SSA4 HSP70 gene of Saccharomyces cerevisiae."
    Boorstein W.R., Craig E.A.
    J. Biol. Chem. 265:18912-18921(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: DS10.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  5. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-552, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiHSP74_YEAST
AccessioniPrimary (citable) accession number: P22202
Secondary accession number(s): D3DM10
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: January 23, 2007
Last modified: March 4, 2015
This is version 142 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 17900 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome V
    Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.