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P22200

- KPYC_SOLTU

UniProt

P22200 - KPYC_SOLTU

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Protein

Pyruvate kinase, cytosolic isozyme

Gene
N/A
Organism
Solanum tuberosum (Potato)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + pyruvate = ADP + phosphoenolpyruvate.

Cofactori

Magnesium.
Potassium.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei50 – 501SubstrateBy similarity
Metal bindingi52 – 521PotassiumBy similarity
Metal bindingi54 – 541PotassiumBy similarity
Metal bindingi84 – 841PotassiumBy similarity
Metal bindingi85 – 851Potassium; via carbonyl oxygenBy similarity
Sitei240 – 2401Transition state stabilizerBy similarity
Metal bindingi242 – 2421MagnesiumSequence Analysis
Binding sitei265 – 2651Substrate; via amide nitrogenBy similarity
Metal bindingi266 – 2661MagnesiumBy similarity
Binding sitei266 – 2661Substrate; via amide nitrogenBy similarity
Binding sitei298 – 2981SubstrateBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. magnesium ion binding Source: InterPro
  3. potassium ion binding Source: InterPro
  4. pyruvate kinase activity Source: UniProtKB-EC

GO - Biological processi

  1. glycolytic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Pyruvate

Enzyme and pathway databases

UniPathwayiUPA00109; UER00188.

Names & Taxonomyi

Protein namesi
Recommended name:
Pyruvate kinase, cytosolic isozyme (EC:2.7.1.40)
Short name:
PK
OrganismiSolanum tuberosum (Potato)
Taxonomic identifieri4113 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeSolanoideaeSolaneaeSolanum
ProteomesiUP000011115: Unplaced

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 510510Pyruvate kinase, cytosolic isozymePRO_0000112124Add
BLAST

Proteomic databases

PRIDEiP22200.

Interactioni

Subunit structurei

Homotetramer.

Structurei

3D structure databases

ProteinModelPortaliP22200.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the pyruvate kinase family.Curated

Phylogenomic databases

KOiK00873.

Family and domain databases

Gene3Di2.40.33.10. 1 hit.
3.20.20.60. 2 hits.
3.40.1380.20. 1 hit.
InterProiIPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR015794. Pyrv_Knase_a/b.
IPR018209. Pyrv_Knase_AS.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
IPR015806. Pyrv_Knase_insert_dom.
[Graphical view]
PANTHERiPTHR11817. PTHR11817. 1 hit.
PfamiPF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
[Graphical view]
PRINTSiPR01050. PYRUVTKNASE.
SUPFAMiSSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
SSF52935. SSF52935. 1 hit.
TIGRFAMsiTIGR01064. pyruv_kin. 1 hit.
PROSITEiPS00110. PYRUVATE_KINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P22200-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MANIDIAGIM KDLPNDGRIP KTKIVCTLGP SSRTVPMLEK LLRAGMNVAR
60 70 80 90 100
FNFSHGTHEY HQETLDNLKI AMQNTQILCA VMLDTKGPEI RTGFLTDGKP
110 120 130 140 150
IQLKEGQEIT VSTDYTIKGN EEMISMSYKK LVMDLKPGNT ILCADGTITL
160 170 180 190 200
TVLSCDPPSG TVRCRCENTA TLGERKNVNL PGVVVDLPTL TEKDKEDILE
210 220 230 240 250
WGVPNNIDMI ALSFVRKGSD LVNVRKVLGP HAKRIQLMSK VENQEGVINF
260 270 280 290 300
DEILRETDSF MVARGDLGME IPVEKIFLAQ KMMIYKCNLA GKAVVTATQM
310 320 330 340 350
LESMIKSPAP TRAEATDVAN AVLDGTDCVM LSGESAAGAY PELAVKIMSR
360 370 380 390 400
ICIEAESSLD NEAIFKEMIR CTPLPMSPLE SLASSAVRTA NKARAKLIVV
410 420 430 440 450
LTRGGSTAKL VAKYRPAVPI LSVVVPVLTT DSFDWSISDE TPARHSLVYR
460 470 480 490 500
GLIPLLGEGS AKATDSESTE VILEAALKSA VTRGLCKPGD AVVALHRIGS
510
ASVIKICVVK
Length:510
Mass (Da):55,170
Last modified:August 1, 1991 - v1
Checksum:i1752C9ED920F2854
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti133 – 1331M → V.
Natural varianti169 – 1691T → S.
Natural varianti227 – 2271V → A.
Natural varianti309 – 3091A → R.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X53688 mRNA. Translation: CAA37727.1.
PIRiJC1481.
RefSeqiNP_001275085.1. NM_001288156.1.
UniGeneiStu.18197.

Genome annotation databases

GeneIDi102601328.
KEGGisot:102601328.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X53688 mRNA. Translation: CAA37727.1 .
PIRi JC1481.
RefSeqi NP_001275085.1. NM_001288156.1.
UniGenei Stu.18197.

3D structure databases

ProteinModelPortali P22200.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PRIDEi P22200.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 102601328.
KEGGi sot:102601328.

Phylogenomic databases

KOi K00873.

Enzyme and pathway databases

UniPathwayi UPA00109 ; UER00188 .

Family and domain databases

Gene3Di 2.40.33.10. 1 hit.
3.20.20.60. 2 hits.
3.40.1380.20. 1 hit.
InterProi IPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR015794. Pyrv_Knase_a/b.
IPR018209. Pyrv_Knase_AS.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
IPR015806. Pyrv_Knase_insert_dom.
[Graphical view ]
PANTHERi PTHR11817. PTHR11817. 1 hit.
Pfami PF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
[Graphical view ]
PRINTSi PR01050. PYRUVTKNASE.
SUPFAMi SSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
SSF52935. SSF52935. 1 hit.
TIGRFAMsi TIGR01064. pyruv_kin. 1 hit.
PROSITEi PS00110. PYRUVATE_KINASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning and characterization of a cDNA for the cytosolic isozyme of plant pyruvate kinase: the relationship between the plant and non-plant enzyme."
    Blakeley S.D., Plaxton W.C., Dennis D.T.
    Plant Mol. Biol. 15:665-669(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Kennebec.
    Tissue: Tuber.
  2. "Structure of the gene encoding potato cytosolic pyruvate kinase."
    Cole K.P., Blakeley S.D., Dennis D.T.
    Gene 122:255-261(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Entry informationi

Entry nameiKPYC_SOLTU
AccessioniPrimary (citable) accession number: P22200
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: August 1, 1991
Last modified: October 29, 2014
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3