Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Pyruvate kinase, cytosolic isozyme

Gene
N/A
Organism
Solanum tuberosum (Potato)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + pyruvate = ADP + phosphoenolpyruvate.

Cofactori

Protein has several cofactor binding sites:

Pathwayi: glycolysis

This protein is involved in step 5 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. no protein annotated in this organism
  3. no protein annotated in this organism
  4. no protein annotated in this organism
  5. Pyruvate kinase (PGSC0003DMG400006590), Pyruvate kinase (PGSC0003DMG400025734), Pyruvate kinase (PGSC0003DMG400025734), Pyruvate kinase, cytosolic isozyme, Pyruvate kinase (PGSC0003DMG400025734), Pyruvate kinase (PGSC0003DMG400024220), Pyruvate kinase (PKc), Pyruvate kinase (PGSC0003DMG400028177), Pyruvate kinase (PGSC0003DMG400027321), Pyruvate kinase (PGSC0003DMG400025298), Pyruvate kinase (PGSC0003DMG400010913), Pyruvate kinase (PGSC0003DMG400002690)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei50SubstrateBy similarity1
Metal bindingi52PotassiumBy similarity1
Metal bindingi54PotassiumBy similarity1
Metal bindingi84PotassiumBy similarity1
Metal bindingi85Potassium; via carbonyl oxygenBy similarity1
Sitei240Transition state stabilizerBy similarity1
Metal bindingi242MagnesiumSequence analysis1
Binding sitei265Substrate; via amide nitrogenBy similarity1
Metal bindingi266MagnesiumBy similarity1
Binding sitei266Substrate; via amide nitrogenBy similarity1
Binding sitei298SubstrateBy similarity1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Pyruvate

Enzyme and pathway databases

UniPathwayiUPA00109; UER00188.

Names & Taxonomyi

Protein namesi
Recommended name:
Pyruvate kinase, cytosolic isozyme (EC:2.7.1.40)
Short name:
PK
OrganismiSolanum tuberosum (Potato)
Taxonomic identifieri4113 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeSolanoideaeSolaneaeSolanum
Proteomesi
  • UP000011115 Componenti: Unassembled WGS sequence

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001121241 – 510Pyruvate kinase, cytosolic isozymeAdd BLAST510

Proteomic databases

PRIDEiP22200.

Interactioni

Subunit structurei

Homotetramer.

Protein-protein interaction databases

STRINGi4113.PGSC0003DMT400065094.

Structurei

3D structure databases

ProteinModelPortaliP22200.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the pyruvate kinase family.Curated

Phylogenomic databases

eggNOGiKOG2323. Eukaryota.
COG0469. LUCA.
KOiK00873.

Family and domain databases

Gene3Di2.40.33.10. 1 hit.
3.20.20.60. 2 hits.
3.40.1380.20. 1 hit.
InterProiIPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR015794. Pyrv_Knase_a/b.
IPR018209. Pyrv_Knase_AS.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
IPR015806. Pyrv_Knase_insert_dom.
[Graphical view]
PANTHERiPTHR11817. PTHR11817. 1 hit.
PfamiPF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
[Graphical view]
PRINTSiPR01050. PYRUVTKNASE.
SUPFAMiSSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
SSF52935. SSF52935. 1 hit.
TIGRFAMsiTIGR01064. pyruv_kin. 1 hit.
PROSITEiPS00110. PYRUVATE_KINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P22200-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MANIDIAGIM KDLPNDGRIP KTKIVCTLGP SSRTVPMLEK LLRAGMNVAR
60 70 80 90 100
FNFSHGTHEY HQETLDNLKI AMQNTQILCA VMLDTKGPEI RTGFLTDGKP
110 120 130 140 150
IQLKEGQEIT VSTDYTIKGN EEMISMSYKK LVMDLKPGNT ILCADGTITL
160 170 180 190 200
TVLSCDPPSG TVRCRCENTA TLGERKNVNL PGVVVDLPTL TEKDKEDILE
210 220 230 240 250
WGVPNNIDMI ALSFVRKGSD LVNVRKVLGP HAKRIQLMSK VENQEGVINF
260 270 280 290 300
DEILRETDSF MVARGDLGME IPVEKIFLAQ KMMIYKCNLA GKAVVTATQM
310 320 330 340 350
LESMIKSPAP TRAEATDVAN AVLDGTDCVM LSGESAAGAY PELAVKIMSR
360 370 380 390 400
ICIEAESSLD NEAIFKEMIR CTPLPMSPLE SLASSAVRTA NKARAKLIVV
410 420 430 440 450
LTRGGSTAKL VAKYRPAVPI LSVVVPVLTT DSFDWSISDE TPARHSLVYR
460 470 480 490 500
GLIPLLGEGS AKATDSESTE VILEAALKSA VTRGLCKPGD AVVALHRIGS
510
ASVIKICVVK
Length:510
Mass (Da):55,170
Last modified:August 1, 1991 - v1
Checksum:i1752C9ED920F2854
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti133M → V.1
Natural varianti169T → S.1
Natural varianti227V → A.1
Natural varianti309A → R.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X53688 mRNA. Translation: CAA37727.1.
PIRiJC1481.
RefSeqiNP_001275085.1. NM_001288156.1.
UniGeneiStu.18197.

Genome annotation databases

GeneIDi102601328.
KEGGisot:102601328.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X53688 mRNA. Translation: CAA37727.1.
PIRiJC1481.
RefSeqiNP_001275085.1. NM_001288156.1.
UniGeneiStu.18197.

3D structure databases

ProteinModelPortaliP22200.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi4113.PGSC0003DMT400065094.

Proteomic databases

PRIDEiP22200.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi102601328.
KEGGisot:102601328.

Phylogenomic databases

eggNOGiKOG2323. Eukaryota.
COG0469. LUCA.
KOiK00873.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00188.

Family and domain databases

Gene3Di2.40.33.10. 1 hit.
3.20.20.60. 2 hits.
3.40.1380.20. 1 hit.
InterProiIPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR015794. Pyrv_Knase_a/b.
IPR018209. Pyrv_Knase_AS.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
IPR015806. Pyrv_Knase_insert_dom.
[Graphical view]
PANTHERiPTHR11817. PTHR11817. 1 hit.
PfamiPF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
[Graphical view]
PRINTSiPR01050. PYRUVTKNASE.
SUPFAMiSSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
SSF52935. SSF52935. 1 hit.
TIGRFAMsiTIGR01064. pyruv_kin. 1 hit.
PROSITEiPS00110. PYRUVATE_KINASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiKPYC_SOLTU
AccessioniPrimary (citable) accession number: P22200
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: August 1, 1991
Last modified: November 11, 2015
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.