Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P22200 (KPYC_SOLTU) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 94. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Pyruvate kinase, cytosolic isozyme

Short name=PK
EC=2.7.1.40
OrganismSolanum tuberosum (Potato) [Reference proteome]
Taxonomic identifier4113 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeSolanoideaeSolaneaeSolanum

Protein attributes

Sequence length510 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

ATP + pyruvate = ADP + phosphoenolpyruvate.

Cofactor

Magnesium.

Potassium.

Pathway

Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.

Subunit structure

Homotetramer.

Subcellular location

Cytoplasm.

Sequence similarities

Belongs to the pyruvate kinase family.

Ontologies

Keywords
   Biological processGlycolysis
   Cellular componentCytoplasm
   LigandATP-binding
Magnesium
Metal-binding
Nucleotide-binding
Potassium
Pyruvate
   Molecular functionKinase
Transferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processglycolysis

Inferred from electronic annotation. Source: UniProtKB-UniPathway

response to cadmium ion

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

   Cellular_componentcytosol

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

plasma membrane

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

magnesium ion binding

Inferred from electronic annotation. Source: InterPro

potassium ion binding

Inferred from electronic annotation. Source: InterPro

pyruvate kinase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 510510Pyruvate kinase, cytosolic isozyme
PRO_0000112124

Sites

Metal binding521Potassium By similarity
Metal binding541Potassium By similarity
Metal binding841Potassium By similarity
Metal binding851Potassium; via carbonyl oxygen By similarity
Metal binding2421Magnesium Potential
Metal binding2661Magnesium By similarity
Binding site501Substrate By similarity
Binding site2651Substrate; via amide nitrogen By similarity
Binding site2661Substrate; via amide nitrogen By similarity
Binding site2981Substrate By similarity
Site2401Transition state stabilizer By similarity

Natural variations

Natural variant1331M → V.
Natural variant1691T → S.
Natural variant2271V → A.
Natural variant3091A → R.

Sequences

Sequence LengthMass (Da)Tools
P22200 [UniParc].

Last modified August 1, 1991. Version 1.
Checksum: 1752C9ED920F2854

FASTA51055,170
        10         20         30         40         50         60 
MANIDIAGIM KDLPNDGRIP KTKIVCTLGP SSRTVPMLEK LLRAGMNVAR FNFSHGTHEY 

        70         80         90        100        110        120 
HQETLDNLKI AMQNTQILCA VMLDTKGPEI RTGFLTDGKP IQLKEGQEIT VSTDYTIKGN 

       130        140        150        160        170        180 
EEMISMSYKK LVMDLKPGNT ILCADGTITL TVLSCDPPSG TVRCRCENTA TLGERKNVNL 

       190        200        210        220        230        240 
PGVVVDLPTL TEKDKEDILE WGVPNNIDMI ALSFVRKGSD LVNVRKVLGP HAKRIQLMSK 

       250        260        270        280        290        300 
VENQEGVINF DEILRETDSF MVARGDLGME IPVEKIFLAQ KMMIYKCNLA GKAVVTATQM 

       310        320        330        340        350        360 
LESMIKSPAP TRAEATDVAN AVLDGTDCVM LSGESAAGAY PELAVKIMSR ICIEAESSLD 

       370        380        390        400        410        420 
NEAIFKEMIR CTPLPMSPLE SLASSAVRTA NKARAKLIVV LTRGGSTAKL VAKYRPAVPI 

       430        440        450        460        470        480 
LSVVVPVLTT DSFDWSISDE TPARHSLVYR GLIPLLGEGS AKATDSESTE VILEAALKSA 

       490        500        510 
VTRGLCKPGD AVVALHRIGS ASVIKICVVK 

« Hide

References

[1]"Cloning and characterization of a cDNA for the cytosolic isozyme of plant pyruvate kinase: the relationship between the plant and non-plant enzyme."
Blakeley S.D., Plaxton W.C., Dennis D.T.
Plant Mol. Biol. 15:665-669(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Kennebec.
Tissue: Tuber.
[2]"Structure of the gene encoding potato cytosolic pyruvate kinase."
Cole K.P., Blakeley S.D., Dennis D.T.
Gene 122:255-261(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X53688 mRNA. Translation: CAA37727.1.
PIRJC1481.
RefSeqNP_001275085.1. NM_001288156.1.
UniGeneStu.18197.

3D structure databases

ProteinModelPortalP22200.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEP22200.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID102601328.

Enzyme and pathway databases

UniPathwayUPA00109; UER00188.

Family and domain databases

Gene3D2.40.33.10. 1 hit.
3.20.20.60. 2 hits.
3.40.1380.20. 1 hit.
InterProIPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR015794. Pyrv_Knase_a/b.
IPR018209. Pyrv_Knase_AS.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
IPR015806. Pyrv_Knase_insert_dom.
[Graphical view]
PANTHERPTHR11817. PTHR11817. 1 hit.
PfamPF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
[Graphical view]
PRINTSPR01050. PYRUVTKNASE.
SUPFAMSSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
SSF52935. SSF52935. 1 hit.
TIGRFAMsTIGR01064. pyruv_kin. 1 hit.
PROSITEPS00110. PYRUVATE_KINASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameKPYC_SOLTU
AccessionPrimary (citable) accession number: P22200
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: August 1, 1991
Last modified: March 19, 2014
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways