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Protein

Fructose-bisphosphate aldolase 7, cytosolic

Gene

FBA7

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Plays a key role in glycolysis and gluconeogenesis.By similarity

Catalytic activityi

D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate.By similarity

Pathwayi: glycolysis

This protein is involved in step 4 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose.Curated
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Glucose-6-phosphate isomerase (MJB21.12), Glucose-6-phosphate isomerase (AXX17_At5g40630), Glucose-6-phosphate isomerase (PGI1 (At4g24620)), Glucose-6-phosphate isomerase, cytosolic (PGIC), Glucose-6-phosphate isomerase (AXX17_At4g28500), Glucose-6-phosphate isomerase 1, chloroplastic (PGI1), Glucose-6-phosphate isomerase (PGIC)
  3. ATP-dependent 6-phosphofructokinase 1 (PFK1), ATP-dependent 6-phosphofructokinase (PFK), ATP-dependent 6-phosphofructokinase (PFK), ATP-dependent 6-phosphofructokinase 3 (PFK3), ATP-dependent 6-phosphofructokinase 4, chloroplastic (PFK4), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit alpha (PFP-ALPHA), ATP-dependent 6-phosphofructokinase 2 (PFK2), ATP-dependent 6-phosphofructokinase (PFK), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta 2 (PFP-BETA2), ATP-dependent 6-phosphofructokinase 5, chloroplastic (PFK5), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit alpha (PFP-ALPHA), ATP-dependent 6-phosphofructokinase (PFK), ATP-dependent 6-phosphofructokinase (PFK), ATP-dependent 6-phosphofructokinase (PFK), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit alpha 1 (PFP-ALPHA1), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta (PFP-BETA), ATP-dependent 6-phosphofructokinase 6 (PFK6), ATP-dependent 6-phosphofructokinase (PFK), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit alpha 2 (PFP-ALPHA2), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta (PFP-BETA), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta 1 (PFP-BETA1), ATP-dependent 6-phosphofructokinase (PFK), ATP-dependent 6-phosphofructokinase 7 (PFK7), ATP-dependent 6-phosphofructokinase (PFK)
  4. Fructose-bisphosphate aldolase 5, cytosolic (FBA5), Fructose-bisphosphate aldolase (FBA6), Fructose-bisphosphate aldolase (FBA4), Fructose-bisphosphate aldolase (AXX17_At4g44320), Fructose-bisphosphate aldolase (AXX17_At4g30610), Fructose-bisphosphate aldolase (FBA2), Fructose-bisphosphate aldolase 1, chloroplastic (FBA1), Fructose-bisphosphate aldolase (AXX17_At5g03010), Fructose-bisphosphate aldolase 7, cytosolic (FBA7), Fructose-bisphosphate aldolase 3, chloroplastic (FBA3), Fructose-bisphosphate aldolase 6, cytosolic (FBA6), Fructose-bisphosphate aldolase (FBA7), Fructose-bisphosphate aldolase (AXX17_At2g00100), Fructose-bisphosphate aldolase 2, chloroplastic (FBA2), Fructose-bisphosphate aldolase (At2g21330), Fructose-bisphosphate aldolase (AXX17_At2g33200), Fructose-bisphosphate aldolase (AXX17_At3g47340), Fructose-bisphosphate aldolase (FBA1), Fructose-bisphosphate aldolase (FBA1), Fructose-bisphosphate aldolase (AXX17_At2g16800), Fructose-bisphosphate aldolase (AXX17_At5g03010), Fructose-bisphosphate aldolase 8, cytosolic (FBA8), Fructose-bisphosphate aldolase (FBA7), Fructose-bisphosphate aldolase (AXX17_At4g30600), Fructose-bisphosphate aldolase (AXX17_At5g03010), Fructose-bisphosphate aldolase (AXX17_At3g47340), Fructose-bisphosphate aldolase 4, cytosolic (FBA4)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei52SubstrateBy similarity1
Binding sitei142SubstrateBy similarity1
Active sitei183Proton acceptorBy similarity1
Active sitei225Schiff-base intermediate with dihydroxyacetone-PBy similarity1
Sitei358Necessary for preference for fructose 1,6-bisphosphate over fructose 1-phosphateBy similarity1

GO - Molecular functioni

  • fructose-bisphosphate aldolase activity Source: UniProtKB

GO - Biological processi

  • gluconeogenesis Source: UniProtKB
  • glycolytic process Source: UniProtKB
  • response to hypoxia Source: TAIR

Keywordsi

Molecular functionLyase
Biological processGlycolysis
LigandSchiff base

Enzyme and pathway databases

BioCyciARA:AT4G26520-MONOMER
ReactomeiR-ATH-114608 Platelet degranulation
R-ATH-6798695 Neutrophil degranulation
R-ATH-70171 Glycolysis
R-ATH-70263 Gluconeogenesis
R-ATH-70350 Fructose catabolism
UniPathwayiUPA00109; UER00183

Names & Taxonomyi

Protein namesi
Recommended name:
Fructose-bisphosphate aldolase 7, cytosolicCurated (EC:4.1.2.13Curated)
Short name:
AtFBA71 Publication
Gene namesi
Name:FBA71 Publication
Ordered Locus Names:At4g26520
ORF Names:M3E9.50
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

AraportiAT4G26520
TAIRilocus:2131508 AT4G26520

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002169192 – 358Fructose-bisphosphate aldolase 7, cytosolicAdd BLAST357

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineBy similarity1
Modified residuei68S-glutathionyl cysteine; transientBy similarity1
Modified residuei173S-glutathionyl cysteine; transient; alternateBy similarity1
Modified residuei173S-nitrosocysteine; transient; alternateBy similarity1

Post-translational modificationi

S-glutathionylated at Cys-68 and Cys-173.By similarity
S-nitrosylated at Cys-173.By similarity

Keywords - PTMi

Acetylation, Glutathionylation, S-nitrosylation

Proteomic databases

PaxDbiP22197
PRIDEiP22197

Expressioni

Tissue specificityi

Highly expressed in flowers, and at lower levels in rosettes leaves and cauline leaves.1 Publication

Inductioni

Down-regulated by cadmium (PubMed:16797112). Induced by glucose and sucrose (PubMed:22561114). Induced by drought stress (PubMed:22561114).2 Publications

Gene expression databases

ExpressionAtlasiP22197 baseline and differential
GenevisibleiP22197 AT

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi3702.AT4G26520.1

Structurei

3D structure databases

ProteinModelPortaliP22197
SMRiP22197
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni266 – 268Substrate bindingBy similarity3

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1557 Eukaryota
COG3588 LUCA
HOGENOMiHOG000220876
InParanoidiP22197
KOiK01623
OMAiYIATPGR
OrthoDBiEOG09360ENE
PhylomeDBiP22197

Family and domain databases

Gene3Di3.20.20.70, 1 hit
InterProiView protein in InterPro
IPR029768 Aldolase_I_AS
IPR013785 Aldolase_TIM
IPR000741 FBA_I
PfamiView protein in Pfam
PF00274 Glycolytic, 1 hit
PROSITEiView protein in PROSITE
PS00158 ALDOLASE_CLASS_I, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P22197-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSAFVSKYED ELIKTAKYIA TPGRGILAAD ESTETIGKRF AGINVENTES
60 70 80 90 100
NRQAYRELLF TSPGSYPCLS GVILFEETLY QKTSDGKPFV DLLMENGVIP
110 120 130 140 150
GIKVDKGLVD LAGTNGETTT QGLDSLGARC QQYYEAGARF AKWRAFFKIG
160 170 180 190 200
ATEPSVLSIQ EDARVLARYA IICQENGLVP IVEPEVLTGG SHDIKKCAAV
210 220 230 240 250
TETVLAAVFK ALNYHHVLLE GTLLKPNMVT PGSDSPKVAP ELIAEYTVTA
260 270 280 290 300
LRRTVPPAIP GIVFLSGIQR EEQATLNLNA MNKLDVLKPW TLTFSFGGAL
310 320 330 340 350
QQSAIKAWAG KPENVAKAQA KFLTRCKANK DATLGKYTGW ASGDSAAFEN

LVVIGYRY
Length:358
Mass (Da):38,810
Last modified:September 27, 2005 - v2
Checksum:i3588E066D488C9CB
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti138A → G in CAA37226 (PubMed:2103470).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X53058 Genomic DNA Translation: CAA37226.1
AL022223 Genomic DNA Translation: CAA18218.1
AL161565 Genomic DNA Translation: CAB79507.1
CP002687 Genomic DNA Translation: AEE85213.1
BT001927 mRNA Translation: AAN71926.1
PIRiD85307
S11958 ADMU
RefSeqiNP_194382.1, NM_118785.4
UniGeneiAt.32173

Genome annotation databases

EnsemblPlantsiAT4G26520.1; AT4G26520.1; AT4G26520
GeneIDi828758
GrameneiAT4G26520.1; AT4G26520.1; AT4G26520
KEGGiath:AT4G26520

Similar proteinsi

Entry informationi

Entry nameiALFC7_ARATH
AccessioniPrimary (citable) accession number: P22197
Secondary accession number(s): O65582, Q53YH0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: September 27, 2005
Last modified: May 23, 2018
This is version 136 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

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