P22192 (PAC1_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Double-strand-specific pac1 ribonuclease
EC=3.1.26.3

Alternative name(s):
Protein hcs

Gene names

Name:	pac1
Synonyms:	hcs
ORF Names:	SPBC119.11c

Organism

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) [Reference proteome]

Taxonomic identifier

284812 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces › Schizosaccharomyces pombe

Protein attributes

Sequence length	363 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Digests double-stranded RNA. Converts long double-stranded RNAs into short oligonucleotides, leaving 5'-phosphates on their cleavage products. Probably inhibits mating and meiosis by degrading a specific mRNA required for sexual development. Ref.1
Catalytic activity	Endonucleolytic cleavage to 5'-phosphomonoester.
Cofactor	Magnesium. Ref.6
Sequence similarities	Contains 1 DRBM (double-stranded RNA-binding) domain. Contains 1 RNase III domain.
Biophysicochemical properties	pH dependence: Optimum pH is 8.5. No activity at pH below 6.5 or above 9.5. Ref.6

Ontologies

Keywords
Biological process	Meiosis
Ligand	Magnesium Metal-binding RNA-binding
Molecular function	Endonuclease Hydrolase Nuclease
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	meiosis Inferred from electronic annotation. Source: UniProtKB-KW rRNA catabolic process Inferred from electronic annotation. Source: InterPro rRNA processing Inferred from mutant phenotype PubMed 10445882. Source: PomBase regulation of conjugation with cellular fusion Inferred from mutant phenotype Ref.1. Source: PomBase snRNA processing Inferred from mutant phenotype PubMed 10445882. Source: PomBase
Cellular_component	nucleolus Inferred from sequence orthology. Source: PomBase
Molecular_function	RNA binding Inferred from sequence model. Source: PomBase double-stranded RNA binding Inferred from electronic annotation. Source: InterPro double-stranded RNA-specific ribonuclease activity Inferred from direct assay Ref.1. Source: PomBase metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW ribonuclease III activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 363	363	Double-strand-specific pac1 ribonuclease		PRO_0000180467
Regions
	Domain	139 – 262	124	RNase III
	Domain	285 – 356	72	DRBM
Amino acid modifications
	Modified residue	122	1	Phosphoserine Ref.7
Experimental info
	Mutagenesis	178	1	G → D or S in snm1-1 and ran1-114; loss of activity. Ref.4
	Sequence conflict	144	1	E → K in AAU05314. Ref.4
	Sequence conflict	144	1	E → K in AAU05315. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	P22192 [UniParc]. Last modified August 1, 1991. Version 1. Checksum: BB866CD6AC5AF33A	FASTA	363	41,539
	10 20 30 40 50 60 MGRFKRHHEG DSDSSSSASD SLSRGRRSLG HKRSSHIKNR QYYILEKKIR KLMFAMKALL 70 80 90 100 110 120 EETKHSTKDD VNLVIPGSTW SHIEGVYEML KSRHDRQNEP VIEEPSSHPK NQKNQENNEP 130 140 150 160 170 180 TSEEFEEGEY PPPLPPLRSE KLKEQVFMHI SRAYEIYPNQ SNPNELLDIH NERLEFLGDS 190 200 210 220 230 240 FFNLFTTRII FSKFPQMDEG SLSKLRAKFV GNESADKFAR LYGFDKTLVL SYSAEKDQLR 250 260 270 280 290 300 KSQKVIADTF EAYLGALILD GQEETAFQWV SRLLQPKIAN ITVQRPIDKL AKSKLFHKYS 310 320 330 340 350 360 TLGHIEYRWV DGAGGSAEGY VIACIFNGKE VARAWGANQK DAGSRAAMQA LEVLAKDYSK FAR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"S. pombe pac1+, whose overexpression inhibits sexual development, encodes a ribonuclease III-like RNase." Iino Y., Sugimoto A., Yamamoto M. EMBO J. 10:221-226(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
[2]	"A gene from S. pombe with homology to E. coli RNase III blocks conjugation and sporulation when overexpressed in wild type cells." Xu H.-P., Riggs M., Rodgers L., Wigler M. Nucleic Acids Res. 18:5304-5304(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Cloning, sequencing, prokaryotic expression and in vitro activity analysis of double-stranded RNA-specific nuclease gene from yeast." Zheng Y., Li S. Submitted (JUL-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 2.1459 and 2.274.
[4]	"Rescue of the fission yeast snRNA synthesis mutant snm1 by overexpression of the double-strand-specific Pac1 ribonuclease." Rotondo G., Gillespie M., Frendewey D. Mol. Gen. Genet. 247:698-708(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], MUTAGENESIS OF GLY-178.
[5]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 972 / ATCC 24843.
[6]	"Purification and characterization of the Pac1 ribonuclease of Schizosaccharomyces pombe." Rotondo G., Frendewey D. Nucleic Acids Res. 24:2377-2386(1996) [PubMed] [Europe PMC] [Abstract] Cited for: COFACTOR, BIOPHYSICOCHEMICAL PROPERTIES.
[7]	"Phosphoproteome analysis of fission yeast." Wilson-Grady J.T., Villen J., Gygi S.P. J. Proteome Res. 7:1088-1097(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-122, MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X54998 Genomic DNA. Translation: CAA38745.1. X53769 Genomic DNA. Translation: CAJ14141.1. S78982 Genomic DNA. Translation: AAB34897.1. AY695821 Genomic DNA. Translation: AAU05314.1. AY695822 Genomic DNA. Translation: AAU05315.1. CU329671 Genomic DNA. Translation: CAA17926.1.
PIR	S12605.
RefSeq	NP_595292.1. NM_001021199.2.
3D structure databases
ProteinModelPortal	P22192.
ModBase	Search...
Protein-protein interaction databases
STRING	4896.SPBC119.11c-1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	SPBC119.11c.1; SPBC119.11c.1:pep; SPBC119.11c.
GeneID	2540084.
KEGG	spo:SPBC119.11c.
Organism-specific databases
PomBase	SPBC119.11c.
Phylogenomic databases
KO	K03685.
OMA	PKIANIT.
OrthoDB	EOG40ZV6Z.
Gene expression databases
GermOnline	SPBC119.11c. Schizosaccharomyces pombe.
Family and domain databases
Gene3D	1.10.1520.10. 1 hit. 3.30.160.20. 1 hit.
InterPro	IPR001159. Ds-RNA-bd. IPR014720. dsRNA-bd-like_dom. IPR011907. RNase_III. IPR000999. RNase_III_dom. [Graphical view]
PANTHER	PTHR11207. PTHR11207. 1 hit.
Pfam	PF00035. dsrm. 1 hit. PF00636. Ribonuclease_3. 1 hit. [Graphical view]
SMART	SM00358. DSRM. 1 hit. SM00535. RIBOc. 1 hit. [Graphical view]
SUPFAM	SSF69065. RNase_III. 1 hit.
PROSITE	PS50137. DS_RBD. 1 hit. PS00517. RNASE_3_1. 1 hit. PS50142. RNASE_3_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	20801221.

Entry information

Entry name

PAC1_SCHPO

Accession

Primary (citable) accession number: P22192
Secondary accession number(s): Q4JFF8, Q670L9

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1991
Last sequence update:	August 1, 1991
Last modified:	May 1, 2013
This is version 100 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program