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P22192 (PAC1_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 108. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Double-strand-specific pac1 ribonuclease

EC=3.1.26.3
Alternative name(s):
Protein hcs
Gene names
Name:pac1
Synonyms:hcs
ORF Names:SPBC119.11c
OrganismSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) [Reference proteome]
Taxonomic identifier284812 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces

Protein attributes

Sequence length363 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Digests double-stranded RNA. Converts long double-stranded RNAs into short oligonucleotides, leaving 5'-phosphates on their cleavage products. Probably inhibits mating and meiosis by degrading a specific mRNA required for sexual development. Ref.1

Catalytic activity

Endonucleolytic cleavage to 5'-phosphomonoester.

Cofactor

Magnesium. Ref.6

Sequence similarities

Contains 1 DRBM (double-stranded RNA-binding) domain.

Contains 1 RNase III domain.

Biophysicochemical properties

pH dependence:

Optimum pH is 8.5. No activity at pH below 6.5 or above 9.5. Ref.6

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 363363Double-strand-specific pac1 ribonuclease
PRO_0000180467

Regions

Domain139 – 262124RNase III
Domain285 – 35672DRBM

Amino acid modifications

Modified residue1221Phosphoserine Ref.7

Experimental info

Mutagenesis1781G → D or S in snm1-1 and ran1-114; loss of activity. Ref.4
Sequence conflict1441E → K in AAU05314. Ref.4
Sequence conflict1441E → K in AAU05315. Ref.4

Sequences

Sequence LengthMass (Da)Tools
P22192 [UniParc].

Last modified August 1, 1991. Version 1.
Checksum: BB866CD6AC5AF33A

FASTA36341,539
        10         20         30         40         50         60 
MGRFKRHHEG DSDSSSSASD SLSRGRRSLG HKRSSHIKNR QYYILEKKIR KLMFAMKALL 

        70         80         90        100        110        120 
EETKHSTKDD VNLVIPGSTW SHIEGVYEML KSRHDRQNEP VIEEPSSHPK NQKNQENNEP 

       130        140        150        160        170        180 
TSEEFEEGEY PPPLPPLRSE KLKEQVFMHI SRAYEIYPNQ SNPNELLDIH NERLEFLGDS 

       190        200        210        220        230        240 
FFNLFTTRII FSKFPQMDEG SLSKLRAKFV GNESADKFAR LYGFDKTLVL SYSAEKDQLR 

       250        260        270        280        290        300 
KSQKVIADTF EAYLGALILD GQEETAFQWV SRLLQPKIAN ITVQRPIDKL AKSKLFHKYS 

       310        320        330        340        350        360 
TLGHIEYRWV DGAGGSAEGY VIACIFNGKE VARAWGANQK DAGSRAAMQA LEVLAKDYSK 


FAR 

« Hide

References

« Hide 'large scale' references
[1]"S. pombe pac1+, whose overexpression inhibits sexual development, encodes a ribonuclease III-like RNase."
Iino Y., Sugimoto A., Yamamoto M.
EMBO J. 10:221-226(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
[2]"A gene from S. pombe with homology to E. coli RNase III blocks conjugation and sporulation when overexpressed in wild type cells."
Xu H.-P., Riggs M., Rodgers L., Wigler M.
Nucleic Acids Res. 18:5304-5304(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"Cloning, sequencing, prokaryotic expression and in vitro activity analysis of double-stranded RNA-specific nuclease gene from yeast."
Zheng Y., Li S.
Submitted (JUL-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 2.1459 and 2.274.
[4]"Rescue of the fission yeast snRNA synthesis mutant snm1 by overexpression of the double-strand-specific Pac1 ribonuclease."
Rotondo G., Gillespie M., Frendewey D.
Mol. Gen. Genet. 247:698-708(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], MUTAGENESIS OF GLY-178.
[5]"The genome sequence of Schizosaccharomyces pombe."
Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. expand/collapse author list , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 972 / ATCC 24843.
[6]"Purification and characterization of the Pac1 ribonuclease of Schizosaccharomyces pombe."
Rotondo G., Frendewey D.
Nucleic Acids Res. 24:2377-2386(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: COFACTOR, BIOPHYSICOCHEMICAL PROPERTIES.
[7]"Phosphoproteome analysis of fission yeast."
Wilson-Grady J.T., Villen J., Gygi S.P.
J. Proteome Res. 7:1088-1097(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-122, IDENTIFICATION BY MASS SPECTROMETRY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X54998 Genomic DNA. Translation: CAA38745.1.
X53769 Genomic DNA. Translation: CAJ14141.1.
S78982 Genomic DNA. Translation: AAB34897.1.
AY695821 Genomic DNA. Translation: AAU05314.1.
AY695822 Genomic DNA. Translation: AAU05315.1.
CU329671 Genomic DNA. Translation: CAA17926.1.
PIRS12605.
RefSeqNP_595292.1. NM_001021199.2.

3D structure databases

ProteinModelPortalP22192.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid276622. 3 interactions.
MINTMINT-4687690.
STRING4896.SPBC119.11c-1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiSPBC119.11c.1; SPBC119.11c.1:pep; SPBC119.11c.
GeneID2540084.
KEGGspo:SPBC119.11c.

Organism-specific databases

PomBaseSPBC119.11c.

Phylogenomic databases

KOK03685.
OMAPKIANIT.
OrthoDBEOG7M0P26.
PhylomeDBP22192.

Family and domain databases

Gene3D1.10.1520.10. 1 hit.
3.30.160.20. 1 hit.
InterProIPR014720. dsRNA-bd_dom.
IPR011907. RNase_III.
IPR000999. RNase_III_dom.
[Graphical view]
PANTHERPTHR11207. PTHR11207. 1 hit.
PfamPF00035. dsrm. 1 hit.
PF14622. Ribonucleas_3_3. 1 hit.
[Graphical view]
SMARTSM00358. DSRM. 1 hit.
SM00535. RIBOc. 1 hit.
[Graphical view]
SUPFAMSSF69065. SSF69065. 1 hit.
PROSITEPS50137. DS_RBD. 1 hit.
PS00517. RNASE_3_1. 1 hit.
PS50142. RNASE_3_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20801221.
PROP22192.

Entry information

Entry namePAC1_SCHPO
AccessionPrimary (citable) accession number: P22192
Secondary accession number(s): Q4JFF8, Q670L9
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: August 1, 1991
Last modified: April 16, 2014
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Schizosaccharomyces pombe

Schizosaccharomyces pombe: entries and gene names