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P22192

- PAC1_SCHPO

UniProt

P22192 - PAC1_SCHPO

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Protein

Double-strand-specific pac1 ribonuclease

Gene

pac1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Digests double-stranded RNA. Converts long double-stranded RNAs into short oligonucleotides, leaving 5'-phosphates on their cleavage products. Probably inhibits mating and meiosis by degrading a specific mRNA required for sexual development.1 Publication

Catalytic activityi

Endonucleolytic cleavage to 5'-phosphomonoester.

Cofactori

Magnesium.1 Publication

pH dependencei

Optimum pH is 8.5. No activity at pH below 6.5 or above 9.5.1 Publication

GO - Molecular functioni

  1. double-stranded RNA binding Source: PomBase
  2. double-stranded RNA-specific ribonuclease activity Source: PomBase
  3. metal ion binding Source: UniProtKB-KW
  4. ribonuclease III activity Source: PomBase
  5. RNA stem-loop binding Source: PomBase

GO - Biological processi

  1. endonucleolytic cleavage involved in rRNA processing Source: PomBase
  2. meiotic nuclear division Source: UniProtKB-KW
  3. regulation of conjugation with cellular fusion Source: PomBase
  4. RNA metabolic process Source: PomBase
  5. rRNA catabolic process Source: InterPro
  6. rRNA processing Source: PomBase
  7. U2 snRNA 3'-end processing Source: PomBase
  8. U4 snRNA 3'-end processing Source: PomBase
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

Meiosis

Keywords - Ligandi

Magnesium, Metal-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Double-strand-specific pac1 ribonuclease (EC:3.1.26.3)
Alternative name(s):
Protein hcs
Gene namesi
Name:pac1
Synonyms:hcs
ORF Names:SPBC119.11c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
ProteomesiUP000002485: Chromosome II

Organism-specific databases

PomBaseiSPBC119.11c.

Subcellular locationi

GO - Cellular componenti

  1. nucleolus Source: PomBase
  2. nucleus Source: PomBase
Complete GO annotation...

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi178 – 1781G → D or S in snm1-1 and ran1-114; loss of activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 363363Double-strand-specific pac1 ribonucleasePRO_0000180467Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei122 – 1221Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP22192.

Interactioni

Protein-protein interaction databases

BioGridi276622. 2 interactions.
MINTiMINT-4687690.
STRINGi4896.SPBC119.11c-1.

Structurei

3D structure databases

ProteinModelPortaliP22192.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini139 – 262124RNase IIIPROSITE-ProRule annotationAdd
BLAST
Domaini285 – 35672DRBMPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 DRBM (double-stranded RNA-binding) domain.PROSITE-ProRule annotation
Contains 1 RNase III domain.PROSITE-ProRule annotation

Phylogenomic databases

InParanoidiP22192.
KOiK03685.
OMAiPKIANIT.
OrthoDBiEOG7M0P26.
PhylomeDBiP22192.

Family and domain databases

Gene3Di1.10.1520.10. 1 hit.
3.30.160.20. 1 hit.
InterProiIPR014720. dsRNA-bd_dom.
IPR011907. RNase_III.
IPR000999. RNase_III_dom.
[Graphical view]
PANTHERiPTHR11207. PTHR11207. 1 hit.
PfamiPF00035. dsrm. 1 hit.
PF14622. Ribonucleas_3_3. 1 hit.
[Graphical view]
SMARTiSM00358. DSRM. 1 hit.
SM00535. RIBOc. 1 hit.
[Graphical view]
SUPFAMiSSF69065. SSF69065. 1 hit.
PROSITEiPS50137. DS_RBD. 1 hit.
PS00517. RNASE_3_1. 1 hit.
PS50142. RNASE_3_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P22192-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGRFKRHHEG DSDSSSSASD SLSRGRRSLG HKRSSHIKNR QYYILEKKIR
60 70 80 90 100
KLMFAMKALL EETKHSTKDD VNLVIPGSTW SHIEGVYEML KSRHDRQNEP
110 120 130 140 150
VIEEPSSHPK NQKNQENNEP TSEEFEEGEY PPPLPPLRSE KLKEQVFMHI
160 170 180 190 200
SRAYEIYPNQ SNPNELLDIH NERLEFLGDS FFNLFTTRII FSKFPQMDEG
210 220 230 240 250
SLSKLRAKFV GNESADKFAR LYGFDKTLVL SYSAEKDQLR KSQKVIADTF
260 270 280 290 300
EAYLGALILD GQEETAFQWV SRLLQPKIAN ITVQRPIDKL AKSKLFHKYS
310 320 330 340 350
TLGHIEYRWV DGAGGSAEGY VIACIFNGKE VARAWGANQK DAGSRAAMQA
360
LEVLAKDYSK FAR
Length:363
Mass (Da):41,539
Last modified:August 1, 1991 - v1
Checksum:iBB866CD6AC5AF33A
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti144 – 1441E → K in AAU05314. (PubMed:7616961)Curated
Sequence conflicti144 – 1441E → K in AAU05315. (PubMed:7616961)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X54998 Genomic DNA. Translation: CAA38745.1.
X53769 Genomic DNA. Translation: CAJ14141.1.
S78982 Genomic DNA. Translation: AAB34897.1.
AY695821 Genomic DNA. Translation: AAU05314.1.
AY695822 Genomic DNA. Translation: AAU05315.1.
CU329671 Genomic DNA. Translation: CAA17926.1.
PIRiS12605.
RefSeqiNP_595292.1. NM_001021199.2.

Genome annotation databases

EnsemblFungiiSPBC119.11c.1; SPBC119.11c.1:pep; SPBC119.11c.
GeneIDi2540084.
KEGGispo:SPBC119.11c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X54998 Genomic DNA. Translation: CAA38745.1 .
X53769 Genomic DNA. Translation: CAJ14141.1 .
S78982 Genomic DNA. Translation: AAB34897.1 .
AY695821 Genomic DNA. Translation: AAU05314.1 .
AY695822 Genomic DNA. Translation: AAU05315.1 .
CU329671 Genomic DNA. Translation: CAA17926.1 .
PIRi S12605.
RefSeqi NP_595292.1. NM_001021199.2.

3D structure databases

ProteinModelPortali P22192.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 276622. 2 interactions.
MINTi MINT-4687690.
STRINGi 4896.SPBC119.11c-1.

Proteomic databases

MaxQBi P22192.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii SPBC119.11c.1 ; SPBC119.11c.1:pep ; SPBC119.11c .
GeneIDi 2540084.
KEGGi spo:SPBC119.11c.

Organism-specific databases

PomBasei SPBC119.11c.

Phylogenomic databases

InParanoidi P22192.
KOi K03685.
OMAi PKIANIT.
OrthoDBi EOG7M0P26.
PhylomeDBi P22192.

Miscellaneous databases

NextBioi 20801221.
PROi P22192.

Family and domain databases

Gene3Di 1.10.1520.10. 1 hit.
3.30.160.20. 1 hit.
InterProi IPR014720. dsRNA-bd_dom.
IPR011907. RNase_III.
IPR000999. RNase_III_dom.
[Graphical view ]
PANTHERi PTHR11207. PTHR11207. 1 hit.
Pfami PF00035. dsrm. 1 hit.
PF14622. Ribonucleas_3_3. 1 hit.
[Graphical view ]
SMARTi SM00358. DSRM. 1 hit.
SM00535. RIBOc. 1 hit.
[Graphical view ]
SUPFAMi SSF69065. SSF69065. 1 hit.
PROSITEi PS50137. DS_RBD. 1 hit.
PS00517. RNASE_3_1. 1 hit.
PS50142. RNASE_3_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "S. pombe pac1+, whose overexpression inhibits sexual development, encodes a ribonuclease III-like RNase."
    Iino Y., Sugimoto A., Yamamoto M.
    EMBO J. 10:221-226(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
  2. "A gene from S. pombe with homology to E. coli RNase III blocks conjugation and sporulation when overexpressed in wild type cells."
    Xu H.-P., Riggs M., Rodgers L., Wigler M.
    Nucleic Acids Res. 18:5304-5304(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Cloning, sequencing, prokaryotic expression and in vitro activity analysis of double-stranded RNA-specific nuclease gene from yeast."
    Zheng Y., Li S.
    Submitted (JUL-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 2.1459 and 2.274.
  4. "Rescue of the fission yeast snRNA synthesis mutant snm1 by overexpression of the double-strand-specific Pac1 ribonuclease."
    Rotondo G., Gillespie M., Frendewey D.
    Mol. Gen. Genet. 247:698-708(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], MUTAGENESIS OF GLY-178.
  5. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  6. "Purification and characterization of the Pac1 ribonuclease of Schizosaccharomyces pombe."
    Rotondo G., Frendewey D.
    Nucleic Acids Res. 24:2377-2386(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: COFACTOR, BIOPHYSICOCHEMICAL PROPERTIES.
  7. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-122, IDENTIFICATION BY MASS SPECTROMETRY.

Entry informationi

Entry nameiPAC1_SCHPO
AccessioniPrimary (citable) accession number: P22192
Secondary accession number(s): Q4JFF8, Q670L9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: August 1, 1991
Last modified: October 29, 2014
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3