Reviewed, UniProtKB/Swiss-Prot P22192 (PAC1_SCHPO)

Names and origin

Protein names

Recommended name:
    Double-strand-specific pac1 ribonuclease
    EC=3.1.26.3
Alternative name(s):
    Protein hcs

Gene names

Name:	pac1
Synonyms:	hcs
ORF Names:	SPBC119.11c

Organism

Schizosaccharomyces pombe (Fission yeast) [Complete proteome]

Taxonomic identifier

4896 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	363 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Digests double-stranded RNA. Converts long double-stranded RNAs into short oligonucleotides, leaving 5'-phosphates on their cleavage products. Probably inhibits mating and meiosis by degrading a specific mRNA required for sexual development. Ref.1
Catalytic activity	Endonucleolytic cleavage to 5'-phosphomonoester.
Cofactor	Magnesium. Ref.6
Sequence similarities	Contains 1 DRBM (double-stranded RNA-binding) domain. Contains 1 RNase III domain.
Biophysicochemical properties	pH dependence: Optimum pH is 8.5. No activity at pH below 6.5 or above 9.5.

Ontologies

Keywords
Biological process	Meiosis
Ligand	Magnesium Metal-binding RNA-binding
Molecular function	Endonuclease Hydrolase Nuclease
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	meiosis Inferred from electronic annotation. Source: UniProtKB-KW rRNA processing Inferred from mutant phenotype. Source: GeneDB_SPombe regulation of conjugation with cellular fusion Ref.1 Inferred from mutant phenotype. Source: GeneDB_SPombe snRNA processing Inferred from mutant phenotype. Source: GeneDB_SPombe
Cellular component	nucleus Inferred from direct assay. Source: GeneDB_SPombe
Molecular function	double-stranded RNA binding Inferred from electronic annotation. Source: InterPro magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW ribonuclease III activity Traceable author statement. Source: GeneDB_SPombe
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 363	363	Double-strand-specific pac1 ribonuclease		PRO_0000180467
Regions
	Domain	139 – 262	124	RNase III
	Domain	285 – 356	72	DRBM
Amino acid modifications
	Modified residue	122	1	Phosphoserine Ref.7
Experimental info
	Mutagenesis	178	1	G → D or S in snm1-1 and ran1-114; loss of activity. Ref.4
	Sequence conflict	144	1	E → K in AAU05314. Ref.4
	Sequence conflict	144	1	E → K in AAU05315. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	P22192-1 [UniParc]. Last modified August 1, 1991. Version 1. Checksum: BB866CD6AC5AF33A	FASTA	363	41,539
	10 20 30 40 50 60 MGRFKRHHEG DSDSSSSASD SLSRGRRSLG HKRSSHIKNR QYYILEKKIR KLMFAMKALL 70 80 90 100 110 120 EETKHSTKDD VNLVIPGSTW SHIEGVYEML KSRHDRQNEP VIEEPSSHPK NQKNQENNEP 130 140 150 160 170 180 TSEEFEEGEY PPPLPPLRSE KLKEQVFMHI SRAYEIYPNQ SNPNELLDIH NERLEFLGDS 190 200 210 220 230 240 FFNLFTTRII FSKFPQMDEG SLSKLRAKFV GNESADKFAR LYGFDKTLVL SYSAEKDQLR 250 260 270 280 290 300 KSQKVIADTF EAYLGALILD GQEETAFQWV SRLLQPKIAN ITVQRPIDKL AKSKLFHKYS 310 320 330 340 350 360 TLGHIEYRWV DGAGGSAEGY VIACIFNGKE VARAWGANQK DAGSRAAMQA LEVLAKDYSK FAR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"S. pombe pac1+, whose overexpression inhibits sexual development, encodes a ribonuclease III-like RNase." Iino Y., Sugimoto A., Yamamoto M. EMBO J. 10:221-226(1991) [PubMed: 1989884] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
[2]	"A gene from S. pombe with homology to E. coli RNase III blocks conjugation and sporulation when overexpressed in wild type cells." Xu H.-P., Riggs M., Rodgers L., Wigler M. Nucleic Acids Res. 18:5304-5304(1990) [PubMed: 2205842] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Cloning, sequencing, prokaryotic expression and in vitro activity analysis of double-stranded RNA-specific nuclease gene from yeast." Zheng Y., Li S. Submitted (JUL-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 2.1459 and 2.274.
[4]	"Rescue of the fission yeast snRNA synthesis mutant snm1 by overexpression of the double-strand-specific Pac1 ribonuclease." Rotondo G., Gillespie M., Frendewey D. Mol. Gen. Genet. 247:698-708(1995) [PubMed: 7616961] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], MUTAGENESIS OF GLY-178.
[5]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 38366 / 972.
[6]	"Purification and characterization of the Pac1 ribonuclease of Schizosaccharomyces pombe." Rotondo G., Frendewey D. Nucleic Acids Res. 24:2377-2386(1996) [PubMed: 8710510] [Abstract] Cited for: COFACTOR, BIOPHYSICOCHEMICAL PROPERTIES.
[7]	"Phosphoproteome analysis of fission yeast." Wilson-Grady J.T., Villen J., Gygi S.P. J. Proteome Res. 7:1088-1097(2008) [PubMed: 18257517] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-122, MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X54998 Genomic DNA. Translation: CAA38745.1. X53769 Genomic DNA. Translation: CAJ14141.1. S78982 Genomic DNA. Translation: AAB34897.1. AY695821 Genomic DNA. Translation: AAU05314.1. AY695822 Genomic DNA. Translation: AAU05315.1. CU329671 Genomic DNA. Translation: CAA17926.1.
PIR	S12605.
RefSeq	NP_595292.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	P22192.
Genome annotation databases
GeneID	2540084.
GenomeReviews	Gene locus pac1 in contig CU329671_GR.
KEGG	spo:SPBC119.11c.
NMPDR	fig|4896.1.peg.1158.
Organism-specific databases
GeneDB_Spombe	SPBC119.11c.
Enzyme and pathway databases
BioCyc	SPOM-XXX-01:SPOM-XXX-01-003374-MON.
BRENDA	3.1.26.3. 653.
Gene expression databases
ArrayExpress	P22192.
GermOnline	SPBC119.11c. Schizosaccharomyces pombe.
Family and domain databases
InterPro	IPR001159. Ds-RNA_bd. IPR000999. RNase_III. [Graphical view]
Gene3D	G3DSA:1.10.1520.10. RNase_III. 1 hit.
Pfam	PF00035. dsrm. 1 hit. PF00636. Ribonuclease_3. 1 hit. [Graphical view]
SMART	SM00358. DSRM. 1 hit. SM00535. RIBOc. 1 hit. [Graphical view]
PROSITE	PS50137. DS_RBD. 1 hit. PS00517. RNASE_3_1. 1 hit. PS50142. RNASE_3_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PAC1_SCHPO

Accession

Primary (citable) accession number: P22192
Secondary accession number(s): Q4JFF8, Q670L9

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1991
Last sequence update:	August 1, 1991
Last modified:	September 22, 2009
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)