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P22185

- AOX1_TYPVN

UniProt

P22185 - AOX1_TYPVN

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Protein

Ubiquinol oxidase 1, mitochondrial

Gene

AOX1

Organism
Typhonium venosum (Voodoo lily) (Sauromatum guttatum)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the cyanide-resistant oxidation of ubiquinol and the reduction of molecular oxygen to water, but does not translocate protons and consequently is not linked to oxidative phosphorylation. May increase respiration when the cytochrome respiratory pathway is restricted, or in response to low temperatures. In S.guttatum the alternative respiratory pathway is thermogenic.2 Publications

Catalytic activityi

2 ubiquinol + O2 = 2 ubiquinone + 2 H2O.

Cofactori

Binds 2 iron ions per subunit.1 Publication

Enzyme regulationi

Not sensitive to pyruvate. Is in a constitutively active state. When the two monomeric subunits are covalently linked by a S-S bond, the enzyme is essentially inactive.2 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi178 – 1781Iron 1Sequence Analysis
Metal bindingi217 – 2171Iron 1Sequence Analysis
Metal bindingi217 – 2171Iron 2Sequence Analysis
Metal bindingi220 – 2201Iron 1Sequence Analysis
Metal bindingi269 – 2691Iron 2Sequence Analysis
Metal bindingi319 – 3191Iron 1Sequence Analysis
Metal bindingi319 – 3191Iron 2Sequence Analysis
Metal bindingi322 – 3221Iron 2Sequence Analysis

GO - Molecular functioni

  1. alternative oxidase activity Source: InterPro
  2. metal ion binding Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Respiratory chain, Transport

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-10382.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquinol oxidase 1, mitochondrial (EC:1.10.3.11)
Alternative name(s):
Alternative oxidase 1
Gene namesi
Name:AOX1
OrganismiTyphonium venosum (Voodoo lily) (Sauromatum guttatum)
Taxonomic identifieri4463 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaAraceaeAroideaeAreaeTyphonium

Subcellular locationi

Mitochondrion inner membrane 1 Publication; Multi-pass membrane protein 1 Publication
Note: Mitochondrial, possibly in the inner surface of the inner mitochondrial membrane.

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. mitochondrial inner membrane Source: UniProtKB-KW
  3. respiratory chain Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi172 – 1721C → A: No effect. 1 Publication
Mutagenesisi217 – 2171E → A: Loss of activity. 1 Publication
Mutagenesisi253 – 2531Y → F: No effect. 1 Publication
Mutagenesisi270 – 2701E → N: Loss of activity. 1 Publication
Mutagenesisi275 – 2751Y → F: Loss of activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 6363MitochondrionSequence AnalysisAdd
BLAST
Chaini64 – 349286Ubiquinol oxidase 1, mitochondrialPRO_0000001736Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi122 – 122InterchainSequence Analysis

Keywords - PTMi

Disulfide bond

Expressioni

Inductioni

By salicylic acid.1 Publication

Interactioni

Subunit structurei

Homodimer; disulfide-linked.1 Publication

Structurei

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei174 – 19421HelicalSequence AnalysisAdd
BLAST
Transmembranei236 – 25621HelicalSequence AnalysisAdd
BLAST

Family & Domainsi

Sequence similaritiesi

Belongs to the alternative oxidase family.Curated

Keywords - Domaini

Transit peptide, Transmembrane, Transmembrane helix

Family and domain databases

InterProiIPR002680. AOX.
[Graphical view]
PfamiPF01786. AOX. 1 hit.
[Graphical view]
PIRSFiPIRSF005229. AOX. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P22185-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MMSSRLVGTA LCRQLSHVPV PQYLPALRPT ADTASSLLHG CSAAAPAQRA
60 70 80 90 100
GLWPPSWFSP PRHASTLSAP AQDGGKEKAA GTAGKVPPGE DGGAEKEAVV
110 120 130 140 150
SYWAVPPSKV SKEDGSEWRW TCFRPWETYQ ADLSIDLHKH HVPTTILDKL
160 170 180 190 200
ALRTVKALRW PTDIFFQRRY ACRAMMLETV AAVPGMVGGV LLHLKSLRRF
210 220 230 240 250
EHSGGWIRAL LEEAENERMH LMTFMEVAQP RWYERALVLA VQGVFFNAYF
260 270 280 290 300
LGYLLSPKFA HRVVGYLEEE AIHSYTEFLK DIDSGAIQDC PAPAIALDYW
310 320 330 340
RLPQGSTLRD VVTVVRADEA HHRDVNHFAS DVHYQDLELK TTPAPLGYH
Length:349
Mass (Da):38,931
Last modified:August 1, 1991 - v1
Checksum:i6E70B2B7A858B273
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti2 – 21M → I in CAA78823. (PubMed:8448361)Curated
Sequence conflicti7 – 71V → A in CAA78823. (PubMed:8448361)Curated
Sequence conflicti40 – 401G → R in CAA78823. (PubMed:8448361)Curated
Sequence conflicti70 – 701P → R in CAA78823. (PubMed:8448361)Curated
Sequence conflicti284 – 2841S → N in CAA78823. (PubMed:8448361)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M60330 mRNA. Translation: AAA34048.1.
Z15117 Genomic DNA. Translation: CAA78823.1.
PIRiA39158.
S30143.

Cross-referencesi

Web resourcesi

Protein Spotlight

The beetle's brothel - Issue 107 of July 2009

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M60330 mRNA. Translation: AAA34048.1 .
Z15117 Genomic DNA. Translation: CAA78823.1 .
PIRi A39158.
S30143.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

BioCyci MetaCyc:MONOMER-10382.

Family and domain databases

InterProi IPR002680. AOX.
[Graphical view ]
Pfami PF01786. AOX. 1 hit.
[Graphical view ]
PIRSFi PIRSF005229. AOX. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Isolation and characterization of a cDNA clone encoding an alternative oxidase protein of Sauromatum guttatum (Schott)."
    Rhoads D.M., McIntosh L.
    Proc. Natl. Acad. Sci. U.S.A. 88:2122-2126(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 67-75, FUNCTION.
  2. "The salicylic acid-inducible alternative oxidase gene aox1 and genes encoding pathogenesis-related proteins share regions of sequence similarity in their promoters."
    Rhoads D.M., McIntosh L.
    Plant Mol. Biol. 21:615-624(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], INDUCTION BY SALICYLIC ACID.
  3. "Covalent and noncovalent dimers of the cyanide-resistant alternative oxidase protein in higher plant mitochondria and their relationship to enzyme activity."
    Umbach A.L., Siedow J.N.
    Plant Physiol. 103:845-854(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBUNIT, ENZYME REGULATION.
  4. "The active site of the cyanide-resistant oxidase from plant mitochondria contains a binuclear iron center."
    Siedow J.N., Umbach A.L., Moore A.L.
    FEBS Lett. 362:10-14(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: IRON-BINDING SITES, COFACTOR, 3D-STRUCTURE MODELING.
  5. "A highly conserved glutamate residue (Glu-270) is essential for plant alternative oxidase activity."
    Albury M.S., Affourtit C., Moore A.L.
    J. Biol. Chem. 273:30301-30305(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF GLU-270, SUBCELLULAR LOCATION.
  6. "A revised model of the active site of alternative oxidase."
    Andersson M.E., Nordlund P.
    FEBS Lett. 449:17-22(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: IRON-BINDING SITES.
  7. "Structure of the plant alternative oxidase. Site-directed mutagenesis provides new information on the active site and membrane topology."
    Albury M.S., Affourtit C., Crichton P.G., Moore A.L.
    J. Biol. Chem. 277:1190-1194(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF CSY-172; GLU-217; TYR-253 AND TYR-275.
  8. "Constitutive activity of Sauromatum guttatum alternative oxidase in Schizosaccharomyces pombe implicates residues in addition to conserved cysteines in alpha-keto acid activation."
    Crichton P.G., Affourtit C., Albury M.S., Carre J.E., Moore A.L.
    FEBS Lett. 579:331-336(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: ENZYME REGULATION.

Entry informationi

Entry nameiAOX1_TYPVN
AccessioniPrimary (citable) accession number: P22185
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: August 1, 1991
Last modified: October 29, 2014
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

The 3 alternative oxidase proteins detected in S.guttatum, with apparent MW of 35 kDa, 36 kDa and 37 kDa, may be post-translationally modified products of the same AOX1 gene.

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Protein Spotlight
    Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3