Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Ubiquinol oxidase 1, mitochondrial

Gene

AOX1

Organism
Sauromatum venosum (Voodoo lily) (Typhonium venosum)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the cyanide-resistant oxidation of ubiquinol and the reduction of molecular oxygen to water, but does not translocate protons and consequently is not linked to oxidative phosphorylation. May increase respiration when the cytochrome respiratory pathway is restricted, or in response to low temperatures. In S.guttatum the alternative respiratory pathway is thermogenic.2 Publications

Miscellaneous

The 3 alternative oxidase proteins detected in S.guttatum, with apparent MW of 35 kDa, 36 kDa and 37 kDa, may be post-translationally modified products of the same AOX1 gene.

Catalytic activityi

2 ubiquinol + O2 = 2 ubiquinone + 2 H2O.

Cofactori

Fe cation1 PublicationNote: Binds 2 iron ions per subunit.1 Publication

Enzyme regulationi

Not sensitive to pyruvate. Is in a constitutively active state. When the two monomeric subunits are covalently linked by a S-S bond, the enzyme is essentially inactive.2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi178Iron 1By similarity1
Metal bindingi217Iron 1By similarity1
Metal bindingi217Iron 2By similarity1
Metal bindingi220Iron 1; via pros nitrogenBy similarity1
Metal bindingi268Iron 2By similarity1
Metal bindingi319Iron 1By similarity1
Metal bindingi319Iron 2By similarity1
Metal bindingi322Iron 2By similarity1

GO - Molecular functioni

Keywordsi

Molecular functionOxidoreductase
Biological processElectron transport, Respiratory chain, Transport
LigandIron, Metal-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-10382.
BRENDAi1.10.3.11. 12588.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquinol oxidase 1, mitochondrial (EC:1.10.3.11)
Alternative name(s):
Alternative oxidase 1
Gene namesi
Name:AOX1
OrganismiSauromatum venosum (Voodoo lily) (Typhonium venosum)
Taxonomic identifieri4463 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaAraceaeAroideaeAreaeSauromatum

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei174 – 194HelicalSequence analysisAdd BLAST21
Transmembranei236 – 256HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi172C → A: No effect. 1 Publication1
Mutagenesisi217E → A: Loss of activity. 1 Publication1
Mutagenesisi253Y → F: No effect. 1 Publication1
Mutagenesisi270E → N: Loss of activity. 1 Publication1
Mutagenesisi275Y → F: Loss of activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 63MitochondrionSequence analysisAdd BLAST63
ChainiPRO_000000173664 – 349Ubiquinol oxidase 1, mitochondrialAdd BLAST286

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi122InterchainBy similarity

Keywords - PTMi

Disulfide bond

Expressioni

Inductioni

By salicylic acid.1 Publication

Interactioni

Subunit structurei

Homodimer; disulfide-linked.1 Publication

Family & Domainsi

Sequence similaritiesi

Belongs to the alternative oxidase family.Curated

Keywords - Domaini

Transit peptide, Transmembrane, Transmembrane helix

Family and domain databases

InterProiView protein in InterPro
IPR002680. AOX.
PfamiView protein in Pfam
PF01786. AOX. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P22185-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMSSRLVGTA LCRQLSHVPV PQYLPALRPT ADTASSLLHG CSAAAPAQRA
60 70 80 90 100
GLWPPSWFSP PRHASTLSAP AQDGGKEKAA GTAGKVPPGE DGGAEKEAVV
110 120 130 140 150
SYWAVPPSKV SKEDGSEWRW TCFRPWETYQ ADLSIDLHKH HVPTTILDKL
160 170 180 190 200
ALRTVKALRW PTDIFFQRRY ACRAMMLETV AAVPGMVGGV LLHLKSLRRF
210 220 230 240 250
EHSGGWIRAL LEEAENERMH LMTFMEVAQP RWYERALVLA VQGVFFNAYF
260 270 280 290 300
LGYLLSPKFA HRVVGYLEEE AIHSYTEFLK DIDSGAIQDC PAPAIALDYW
310 320 330 340
RLPQGSTLRD VVTVVRADEA HHRDVNHFAS DVHYQDLELK TTPAPLGYH
Length:349
Mass (Da):38,931
Last modified:August 1, 1991 - v1
Checksum:i6E70B2B7A858B273
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti2M → I in CAA78823 (PubMed:8448361).Curated1
Sequence conflicti7V → A in CAA78823 (PubMed:8448361).Curated1
Sequence conflicti40G → R in CAA78823 (PubMed:8448361).Curated1
Sequence conflicti70P → R in CAA78823 (PubMed:8448361).Curated1
Sequence conflicti284S → N in CAA78823 (PubMed:8448361).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M60330 mRNA. Translation: AAA34048.1.
Z15117 Genomic DNA. Translation: CAA78823.1.
PIRiA39158.
S30143.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiAOX1_SAUVE
AccessioniPrimary (citable) accession number: P22185
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: August 1, 1991
Last modified: June 7, 2017
This is version 94 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Protein Spotlight
    Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
  2. SIMILARITY comments
    Index of protein domains and families