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P22185

- AOX1_TYPVN

UniProt

P22185 - AOX1_TYPVN

Protein

Ubiquinol oxidase 1, mitochondrial

Gene

AOX1

Organism
Typhonium venosum (Voodoo lily) (Sauromatum guttatum)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 85 (01 Oct 2014)
      Sequence version 1 (01 Aug 1991)
      Previous versions | rss
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    Functioni

    Catalyzes the cyanide-resistant oxidation of ubiquinol and the reduction of molecular oxygen to water, but does not translocate protons and consequently is not linked to oxidative phosphorylation. May increase respiration when the cytochrome respiratory pathway is restricted, or in response to low temperatures. In S.guttatum the alternative respiratory pathway is thermogenic.2 Publications

    Catalytic activityi

    2 ubiquinol + O2 = 2 ubiquinone + 2 H2O.

    Cofactori

    Binds 2 iron ions per subunit.1 Publication

    Enzyme regulationi

    Not sensitive to pyruvate. Is in a constitutively active state. When the two monomeric subunits are covalently linked by a S-S bond, the enzyme is essentially inactive.2 Publications

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi178 – 1781Iron 1Sequence Analysis
    Metal bindingi217 – 2171Iron 1Sequence Analysis
    Metal bindingi217 – 2171Iron 2Sequence Analysis
    Metal bindingi220 – 2201Iron 1Sequence Analysis
    Metal bindingi269 – 2691Iron 2Sequence Analysis
    Metal bindingi319 – 3191Iron 1Sequence Analysis
    Metal bindingi319 – 3191Iron 2Sequence Analysis
    Metal bindingi322 – 3221Iron 2Sequence Analysis

    GO - Molecular functioni

    1. alternative oxidase activity Source: InterPro
    2. metal ion binding Source: UniProtKB-KW

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Electron transport, Respiratory chain, Transport

    Keywords - Ligandi

    Iron, Metal-binding

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-10382.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ubiquinol oxidase 1, mitochondrial (EC:1.10.3.11)
    Alternative name(s):
    Alternative oxidase 1
    Gene namesi
    Name:AOX1
    OrganismiTyphonium venosum (Voodoo lily) (Sauromatum guttatum)
    Taxonomic identifieri4463 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaAraceaeAroideaeAreaeTyphonium

    Subcellular locationi

    Mitochondrion inner membrane 1 Publication; Multi-pass membrane protein 1 Publication
    Note: Mitochondrial, possibly in the inner surface of the inner mitochondrial membrane.

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. mitochondrial inner membrane Source: UniProtKB-SubCell
    3. respiratory chain Source: UniProtKB-KW

    Keywords - Cellular componenti

    Membrane, Mitochondrion, Mitochondrion inner membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi172 – 1721C → A: No effect. 1 Publication
    Mutagenesisi217 – 2171E → A: Loss of activity. 1 Publication
    Mutagenesisi253 – 2531Y → F: No effect. 1 Publication
    Mutagenesisi270 – 2701E → N: Loss of activity. 1 Publication
    Mutagenesisi275 – 2751Y → F: Loss of activity. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 6363MitochondrionSequence AnalysisAdd
    BLAST
    Chaini64 – 349286Ubiquinol oxidase 1, mitochondrialPRO_0000001736Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi122 – 122InterchainSequence Analysis

    Keywords - PTMi

    Disulfide bond

    Expressioni

    Inductioni

    By salicylic acid.1 Publication

    Interactioni

    Subunit structurei

    Homodimer; disulfide-linked.1 Publication

    Structurei

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei174 – 19421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei236 – 25621HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the alternative oxidase family.Curated

    Keywords - Domaini

    Transit peptide, Transmembrane, Transmembrane helix

    Family and domain databases

    InterProiIPR002680. AOX.
    [Graphical view]
    PfamiPF01786. AOX. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005229. AOX. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P22185-1 [UniParc]FASTAAdd to Basket

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    MMSSRLVGTA LCRQLSHVPV PQYLPALRPT ADTASSLLHG CSAAAPAQRA    50
    GLWPPSWFSP PRHASTLSAP AQDGGKEKAA GTAGKVPPGE DGGAEKEAVV 100
    SYWAVPPSKV SKEDGSEWRW TCFRPWETYQ ADLSIDLHKH HVPTTILDKL 150
    ALRTVKALRW PTDIFFQRRY ACRAMMLETV AAVPGMVGGV LLHLKSLRRF 200
    EHSGGWIRAL LEEAENERMH LMTFMEVAQP RWYERALVLA VQGVFFNAYF 250
    LGYLLSPKFA HRVVGYLEEE AIHSYTEFLK DIDSGAIQDC PAPAIALDYW 300
    RLPQGSTLRD VVTVVRADEA HHRDVNHFAS DVHYQDLELK TTPAPLGYH 349
    Length:349
    Mass (Da):38,931
    Last modified:August 1, 1991 - v1
    Checksum:i6E70B2B7A858B273
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti2 – 21M → I in CAA78823. (PubMed:8448361)Curated
    Sequence conflicti7 – 71V → A in CAA78823. (PubMed:8448361)Curated
    Sequence conflicti40 – 401G → R in CAA78823. (PubMed:8448361)Curated
    Sequence conflicti70 – 701P → R in CAA78823. (PubMed:8448361)Curated
    Sequence conflicti284 – 2841S → N in CAA78823. (PubMed:8448361)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M60330 mRNA. Translation: AAA34048.1.
    Z15117 Genomic DNA. Translation: CAA78823.1.
    PIRiA39158.
    S30143.

    Cross-referencesi

    Web resourcesi

    Protein Spotlight

    The beetle's brothel - Issue 107 of July 2009

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M60330 mRNA. Translation: AAA34048.1 .
    Z15117 Genomic DNA. Translation: CAA78823.1 .
    PIRi A39158.
    S30143.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    BioCyci MetaCyc:MONOMER-10382.

    Family and domain databases

    InterProi IPR002680. AOX.
    [Graphical view ]
    Pfami PF01786. AOX. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005229. AOX. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Isolation and characterization of a cDNA clone encoding an alternative oxidase protein of Sauromatum guttatum (Schott)."
      Rhoads D.M., McIntosh L.
      Proc. Natl. Acad. Sci. U.S.A. 88:2122-2126(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 67-75, FUNCTION.
    2. "The salicylic acid-inducible alternative oxidase gene aox1 and genes encoding pathogenesis-related proteins share regions of sequence similarity in their promoters."
      Rhoads D.M., McIntosh L.
      Plant Mol. Biol. 21:615-624(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], INDUCTION BY SALICYLIC ACID.
    3. "Covalent and noncovalent dimers of the cyanide-resistant alternative oxidase protein in higher plant mitochondria and their relationship to enzyme activity."
      Umbach A.L., Siedow J.N.
      Plant Physiol. 103:845-854(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBUNIT, ENZYME REGULATION.
    4. "The active site of the cyanide-resistant oxidase from plant mitochondria contains a binuclear iron center."
      Siedow J.N., Umbach A.L., Moore A.L.
      FEBS Lett. 362:10-14(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: IRON-BINDING SITES, COFACTOR, 3D-STRUCTURE MODELING.
    5. "A highly conserved glutamate residue (Glu-270) is essential for plant alternative oxidase activity."
      Albury M.S., Affourtit C., Moore A.L.
      J. Biol. Chem. 273:30301-30305(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: MUTAGENESIS OF GLU-270, SUBCELLULAR LOCATION.
    6. "A revised model of the active site of alternative oxidase."
      Andersson M.E., Nordlund P.
      FEBS Lett. 449:17-22(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: IRON-BINDING SITES.
    7. "Structure of the plant alternative oxidase. Site-directed mutagenesis provides new information on the active site and membrane topology."
      Albury M.S., Affourtit C., Crichton P.G., Moore A.L.
      J. Biol. Chem. 277:1190-1194(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: MUTAGENESIS OF CSY-172; GLU-217; TYR-253 AND TYR-275.
    8. "Constitutive activity of Sauromatum guttatum alternative oxidase in Schizosaccharomyces pombe implicates residues in addition to conserved cysteines in alpha-keto acid activation."
      Crichton P.G., Affourtit C., Albury M.S., Carre J.E., Moore A.L.
      FEBS Lett. 579:331-336(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: ENZYME REGULATION.

    Entry informationi

    Entry nameiAOX1_TYPVN
    AccessioniPrimary (citable) accession number: P22185
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1991
    Last sequence update: August 1, 1991
    Last modified: October 1, 2014
    This is version 85 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    The 3 alternative oxidase proteins detected in S.guttatum, with apparent MW of 35 kDa, 36 kDa and 37 kDa, may be post-translationally modified products of the same AOX1 gene.

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. Protein Spotlight
      Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3