Skip Header

Contribute Send feedback
Read comments (?) or add your own

P22180 (PMA1_SOLLC) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 97. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Plasma membrane ATPase 1
EC=3.6.3.6
Alternative name(s):
Proton pump 1
Gene names
Name:LHA1
OrganismSolanum lycopersicum (Tomato) (Lycopersicon esculentum)
Taxonomic identifier4081 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Solanoideae › Solaneae › Solanum › Lycopersicon

Protein attributes

Sequence length956 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

The plasma membrane ATPase of plants and fungi is a hydrogen ion pump. The proton gradient it generates drives the active transport of nutrients by H+-symport. The resulting external acidification and/or internal alkinization may mediate growth responses.

Catalytic activity

ATP + H2O + H+(In) = ADP + phosphate + H+(Out).

Subunit structure

Possibly exists as an homodimer or an homotrimer.

Subcellular location

Cell membrane; Multi-pass membrane protein.

Miscellaneous

As many as 6 to 8 closely related genes may encode other isoforms of plasma membrane ATPase in tomato, like the LHA2 gene product which is 96% identical to the LHA1 gene product.

Sequence similarities

Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIIA subfamily. [View classification]

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 956956Plasma membrane ATPase 1
PRO_0000046289

Regions

Topological domain1 – 6565Cytoplasmic Potential
Transmembrane66 – 8520Helical; Potential
Topological domain86 – 9712Extracellular Potential
Transmembrane98 – 11821Helical; Potential
Topological domain119 – 247129Cytoplasmic Potential
Transmembrane248 – 26821Helical; Potential
Topological domain269 – 2779Extracellular Potential
Transmembrane278 – 29518Helical; Potential
Topological domain296 – 646351Cytoplasmic Potential
Transmembrane647 – 66620Helical; Potential
Topological domain667 – 6748Extracellular Potential
Transmembrane675 – 69723Helical; Potential
Topological domain698 – 71316Cytoplasmic Potential
Transmembrane714 – 73421Helical; Potential
Topological domain735 – 75925Extracellular Potential
Transmembrane760 – 78021Helical; Potential
Topological domain781 – 79212Cytoplasmic Potential
Transmembrane793 – 81321Helical; Potential
Topological domain814 – 8218Extracellular Potential
Transmembrane822 – 84221Helical; Potential
Topological domain843 – 956114Cytoplasmic Potential

Sites

Active site33314-aspartylphosphate intermediate By similarity
Metal binding5921Magnesium By similarity
Metal binding5961Magnesium By similarity

Sequences

Sequence LengthMass (Da)Tools
P22180 [UniParc].

Last modified August 1, 1991. Version 1.
Checksum: CD23AA8B10E7B6E5

FASTA956105,103
        10         20         30         40         50         60 
MAEKPEVLDA VLKETVDLEN IPIEEVFENL RCTREGLTAT AAQERLSIFG YNKLEEKKES 

        70         80         90        100        110        120 
KFLKFLGFMW NPLSWVMEAA AIMAIALANG GGKPPDWQDF VGIITLLIIN STISFIEENN 

       130        140        150        160        170        180 
AGNAAAALMA RLAPKAKVLR DGKWDEEDAS VLVPGDIISI KLGDIIPADA RLLEGDPLKI 

       190        200        210        220        230        240 
DQSALTGESL PVTKGPGDGV YSGSTCKQGE IEAVVIATGV HTFFGKAAHL VDSTNQVGHF 

       250        260        270        280        290        300 
QKVLTAIGNF CICSIAVGMI IEIIVMYPIQ HRKYRPGIDN LLVLLIGGIP IAMPTVLSVT 

       310        320        330        340        350        360 
MAIGSHRLAQ QGAITKRMTA IEEMAGMDVL CSDKTGTLTL NKLTVDKALI EVFAKGIDAD 

       370        380        390        400        410        420 
TVVLMAARAS RIENQDAIDT AIVGMLADPK EARAGIREIH FLPFNPTDKR TALTYLDGEG 

       430        440        450        460        470        480 
KMHRVSKGAP EQILNLAHNK SDIERRVHTV IDKFAERGLR SLGVAYQEVP EGRKESAGGP 

       490        500        510        520        530        540 
WQFIALLPLF DPPRHDSAET IRRALNLGVN VKMITGDQLA IGKETGRRLG MGTNMYPSSA 

       550        560        570        580        590        600 
LLGQTKDESI AALPIDELIE KADGFAGVFP EHKYEIVKRL QARKHICGMT GDGVNDAPAL 

       610        620        630        640        650        660 
KKADIGIAVD DATDAARSAS DIVLTEPGLS VIISAVLTSR AIFQRMKNYT IYAVSITIRI 

       670        680        690        700        710        720 
VLGFMLLALI WKFDFPPFMV LIIAILNDGT IMTISKDRVK PSPLPDSWKL AEIFTTGVVL 

       730        740        750        760        770        780 
GGYLAMMTVI FFWAAYKTNF FPRIFGVSTL EKTATDDFRK LASAIYLQVS TISQALIFVT 

       790        800        810        820        830        840 
RSRSWSFVER PGLLLVFAFF VAQLVATLIA VYANWSFAAI EGIGWGWAGV IWLYNIVTYI 

       850        860        870        880        890        900 
PLDLIKFLIR YALSGKAWDL VLEQRIAFTR KKDFGKELRE LQWAHAQRTL HGLQVPDPKI 

       910        920        930        940        950 
FSETTNFNEL NQLAEEAKRR AEIARLRELH TLKGHVESVV KLKGLDIETI QQSYTV

« Hide

References

[1]"Molecular cloning of tomato plasma membrane H+-ATPase."
Ewing N.N., Wimmers L.E., Meyer D.J., Chetelat R.T., Bennett A.B.
Plant Physiol. 94:1874-1881(1990) [PubMed: 16667929] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Castlemart.
Tissue: Root.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		M60166 mRNA. Translation: AAA34173.1.
PIRA45506.
RefSeqNP_001234775.1. NM_001247846.1.
UniGeneLes.3671.

3D structure databases

ProteinModelPortalP22180.
SMRP22180. Positions 16-851, 905-956.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID543982.

Family and domain databases

InterProIPR008250. ATPase_P-typ_ATPase-assoc-dom.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023300. ATPase_P-typ_cyto_domA.
IPR023299. ATPase_P-typ_cyto_domN.
IPR000695. ATPase_P-typ_H-transp.
IPR001757. ATPase_P-typ_ion-transptr.
IPR018303. ATPase_P-typ_P_site.
IPR006534. ATPase_P-typ_PM_proton-efflux.
IPR023298. ATPase_P-typ_TM_dom.
IPR005834. Dehalogen-like_hydro.
IPR023214. HAD-like_dom.
[Graphical view]
Gene3DG3DSA:2.70.150.10. ATPase_P-typ_cyto_domA. 2 hits.
G3DSA:3.40.1110.10. ATPase_P-typ_cyto_domN. 1 hit.
G3DSA:1.20.1110.10. ATPase_P-typ_TM_dom. 3 hits.
PANTHERPTHR24093:SF61. PTHR24093:SF61. 1 hit.
PfamPF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
PF00702. Hydrolase. 1 hit.
[Graphical view]
PRINTSPR00119. CATATPASE.
PR00120. HATPASE.
SMARTSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMSSF56784. HAD-like_dom. 1 hit.
TIGRFAMsTIGR01647. ATPase-IIIA_H. 1 hit.
TIGR01494. ATPase_P-type. 2 hits.
PROSITEPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePMA1_SOLLC
AccessionPrimary (citable) accession number: P22180
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: August 1, 1991
Last modified: December 14, 2011
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents