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P22173

- RDRP_IPNVJ

UniProt

P22173 - RDRP_IPNVJ

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Protein

RNA-directed RNA polymerase

Gene

VP1

Organism
Infectious pancreatic necrosis virus (strain Jasper) (IPNV)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

RNA-dependent RNA polymerase which is found both free and covalently attached to the genomic RNA. May also contain guanylyl and methyl transferase activities (By similarity).By similarity

Catalytic activityi

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).PROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi248 – 2558GTPSequence Analysis

GO - Molecular functioni

  1. GTP binding Source: UniProtKB-KW
  2. RNA-directed RNA polymerase activity Source: UniProtKB-KW

GO - Biological processi

  1. RNA-protein covalent cross-linking Source: UniProtKB-KW
  2. viral genome replication Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, RNA-directed RNA polymerase, Transferase

Keywords - Biological processi

Viral RNA replication

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
RNA-directed RNA polymerase (EC:2.7.7.48)
Short name:
RDRP
Alternative name(s):
Protein VP1
Gene namesi
Name:VP1
OrganismiInfectious pancreatic necrosis virus (strain Jasper) (IPNV)
Taxonomic identifieri11003 [NCBI]
Taxonomic lineageiVirusesdsRNA virusesBirnaviridaeAquabirnavirus
Virus hostiOncorhynchus mykiss (Rainbow trout) (Salmo gairdneri) [TaxID: 8022]
Salmo [TaxID: 8028]
ProteomesiUP000007248: Genome

Subcellular locationi

Virion By similarity
Note: Minor amounts are incorporated in the virion.By similarity

GO - Cellular componenti

  1. virion Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 845845RNA-directed RNA polymerasePRO_0000221963Add
BLAST

Post-translational modificationi

Exists in multiple phosphorylated forms.

Keywords - PTMi

Covalent protein-RNA linkage, Phosphoprotein

Interactioni

Subunit structurei

Interacts with VP3 in the cytoplasm.By similarity

Structurei

Secondary structure

1
845
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi14 – 185
Beta strandi23 – 253
Turni28 – 303
Helixi45 – 5410
Beta strandi65 – 728
Helixi73 – 764
Helixi78 – 803
Helixi81 – 855
Beta strandi91 – 10212
Beta strandi111 – 1133
Helixi125 – 13713
Helixi143 – 15614
Beta strandi157 – 1604
Helixi164 – 17916
Beta strandi180 – 1823
Helixi187 – 1904
Helixi194 – 20411
Helixi221 – 2244
Turni231 – 2333
Helixi256 – 27823
Helixi283 – 29311
Helixi295 – 2984
Beta strandi299 – 3046
Beta strandi307 – 3104
Helixi311 – 3133
Turni314 – 3163
Beta strandi320 – 3234
Helixi326 – 34015
Turni347 – 3493
Beta strandi350 – 3545
Turni361 – 3633
Helixi364 – 37714
Beta strandi382 – 3865
Beta strandi389 – 3946
Beta strandi397 – 4037
Helixi406 – 4094
Helixi412 – 42514
Beta strandi434 – 4363
Helixi438 – 4469
Helixi448 – 4525
Beta strandi456 – 4605
Beta strandi462 – 4654
Helixi476 – 49318
Helixi502 – 5054
Helixi507 – 5115
Beta strandi515 – 5217
Helixi524 – 53310
Beta strandi537 – 5393
Turni540 – 5434
Beta strandi556 – 5605
Beta strandi563 – 5686
Turni569 – 5724
Beta strandi573 – 5786
Helixi580 – 5889
Helixi596 – 5994
Helixi604 – 61815
Helixi621 – 6233
Helixi625 – 64521
Helixi650 – 6545
Helixi656 – 6594
Turni661 – 6655
Helixi677 – 6848
Helixi692 – 6943
Beta strandi699 – 7013
Helixi706 – 7149
Turni715 – 7184
Helixi724 – 7329
Helixi737 – 7393
Helixi740 – 75112
Helixi757 – 77115
Helixi775 – 7773
Helixi781 – 79010

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2YI8X-ray2.30A/B/C/D/E1-790[»]
2YI9X-ray2.20A/B/C/D/E1-790[»]
2YIAX-ray3.02A/B/C/D/E/F/G/H1-790[»]
2YIBX-ray3.80A/B/C1-845[»]
D1-687[»]
3ZEDX-ray2.20A/B/C1-845[»]
ProteinModelPortaliP22173.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP22173.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini384 – 588205RdRp catalyticPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi766 – 7694Poly-Gln

Sequence similaritiesi

Contains 1 RdRp catalytic domain.PROSITE-ProRule annotation

Family and domain databases

InterProiIPR007100. RNA-dir_pol_birnavirus.
IPR007098. RNA-dir_pol_mononegavirus.
[Graphical view]
PfamiPF04197. Birna_RdRp. 1 hit.
[Graphical view]
PROSITEiPS50524. RDRP_DSRNA_BIR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P22173-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSDIFNSPQN KASILTALMK STTGDVEDVL IPKRFRPAKD PLDSPQAAAQ
60 70 80 90 100
FLKDNKYRIL RPRAIPTMVE LETDAALPRL RQMVEDGKLK DTVSVPEGTT
110 120 130 140 150
AFYPKYYPFH KPDHDEVGTF GAPDITLLKQ LTFFLLENDF PTGPETLRQV
160 170 180 190 200
REAIATLQYG SGSYSGQLNR LLAMKGVATG RNPNKTPKTV GYTNEQLAKL
210 220 230 240 250
LEQTLPINTP KHEDPDLRWA PSWLINYTGD LSTDKSYLPH VTIKSSAGLP
260 270 280 290 300
YIGKTKGDTT AEALVLADSF IRDLGRAATS ADPEAGVKKT ITDFWYLSCG
310 320 330 340 350
LLFPKGERYT QVDWDKKTRN IWSAPYPTHL LLSMVSTPVM NESKLNITNT
360 370 380 390 400
QTPSLYGFSP FHGGMDRIMT IIRDSLDNDE DLVMIYADNI YILQDNTWYS
410 420 430 440 450
IDLEKGEANC TPQHMQAMMY YLLTRGWTNE DGSPRYNPTW ATFAMNVAPS
460 470 480 490 500
MVVDSSCLLM NLQLKTYGQG SGNAFTFLNN HLMSTIVVAE WVKAGKPNPM
510 520 530 540 550
TKEFMDLEEK TGINFKIERE LKNLRETIVE AVETAPQDGY LADGSDLPPI
560 570 580 590 600
RPGKAVELDL LGWSAIYSRQ MEMFVPVLEN ERLIASAAYP KGLENKALAR
610 620 630 640 650
KPGAEIAYQI VRYEAIRLVG GWNNPLLETA AKHMSLDKRK RLEVKGIDVT
660 670 680 690 700
GFLDDWNNMS EFGGDLEGIT LSEPLTNQTL VDINTPLDSF DPKARPQTPR
710 720 730 740 750
SPKKTLDEVT TAITSGTYKD PKSAVWRLLD QRTKLRVSTL RDQALALKPA
760 770 780 790 800
SSSVDNWAEA TEELAQQQQL LMKANNLLKS SLTETREALE TIQSDKIIAG
810 820 830 840
KSNPEKNPGT AANPVVGYGE FSEKIPLTPT QKKNAKRREK QRRNQ
Length:845
Mass (Da):94,467
Last modified:August 1, 1991 - v1
Checksum:i45C392DF3AAF767A
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M58756 Genomic RNA. Translation: AAA46243.1.
PIRiA37946. RRXSJA.
RefSeqiNP_047197.1. NC_001916.1.

Genome annotation databases

GeneIDi956514.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M58756 Genomic RNA. Translation: AAA46243.1 .
PIRi A37946. RRXSJA.
RefSeqi NP_047197.1. NC_001916.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2YI8 X-ray 2.30 A/B/C/D/E 1-790 [» ]
2YI9 X-ray 2.20 A/B/C/D/E 1-790 [» ]
2YIA X-ray 3.02 A/B/C/D/E/F/G/H 1-790 [» ]
2YIB X-ray 3.80 A/B/C 1-845 [» ]
D 1-687 [» ]
3ZED X-ray 2.20 A/B/C 1-845 [» ]
ProteinModelPortali P22173.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 956514.

Miscellaneous databases

EvolutionaryTracei P22173.

Family and domain databases

InterProi IPR007100. RNA-dir_pol_birnavirus.
IPR007098. RNA-dir_pol_mononegavirus.
[Graphical view ]
Pfami PF04197. Birna_RdRp. 1 hit.
[Graphical view ]
PROSITEi PS50524. RDRP_DSRNA_BIR. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Sequence analysis of infectious pancreatic necrosis virus genome segment B and its encoded VP1 protein: a putative RNA-dependent RNA polymerase lacking the Gly-Asp-Asp motif."
    Duncan R., Mason C.L., Nagy E., Leong J.-A., Dobos P.
    Virology 181:541-552(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Entry informationi

Entry nameiRDRP_IPNVJ
AccessioniPrimary (citable) accession number: P22173
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: August 1, 1991
Last modified: October 29, 2014
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3