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P22173 (RDRP_IPNVJ) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 71. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
RNA-directed RNA polymerase
Short name=RDRP
EC=2.7.7.48
Alternative name(s):
Protein VP1
Gene names
Name:VP1
OrganismInfectious pancreatic necrosis virus (strain Jasper) (IPNV) [Reference proteome]
Taxonomic identifier11003 [NCBI]
Taxonomic lineageViruses › dsRNA viruses › Birnaviridae › Aquabirnavirus ›
Virus hostOncorhynchus mykiss (Rainbow trout) (Salmo gairdneri) [TaxID: 8022]
Salmo [TaxID: 8028]

Protein attributes

Sequence length845 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

RNA-dependent RNA polymerase which is found both free and covalently attached to the genomic RNA. May also contain guanylyl and methyl transferase activities By similarity.

Catalytic activity

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).

Subunit structure

Interacts with VP3 in the cytoplasm By similarity.

Subcellular location

Virion By similarity. Note: Minor amounts are incorporated in the virion By similarity.

Post-translational modification

Exists in multiple phosphorylated forms.

Sequence similarities

Contains 1 RdRp catalytic domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 845845RNA-directed RNA polymerase
PRO_0000221963

Regions

Domain384 – 588205RdRp catalytic
Nucleotide binding248 – 2558GTP Potential
Compositional bias766 – 7694Poly-Gln

Secondary structure

1 ................................................................................................................................... 845
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P22173 [UniParc].

Last modified August 1, 1991. Version 1.
Checksum: 45C392DF3AAF767A

FASTA84594,467
        10         20         30         40         50         60 
MSDIFNSPQN KASILTALMK STTGDVEDVL IPKRFRPAKD PLDSPQAAAQ FLKDNKYRIL 

        70         80         90        100        110        120 
RPRAIPTMVE LETDAALPRL RQMVEDGKLK DTVSVPEGTT AFYPKYYPFH KPDHDEVGTF 

       130        140        150        160        170        180 
GAPDITLLKQ LTFFLLENDF PTGPETLRQV REAIATLQYG SGSYSGQLNR LLAMKGVATG 

       190        200        210        220        230        240 
RNPNKTPKTV GYTNEQLAKL LEQTLPINTP KHEDPDLRWA PSWLINYTGD LSTDKSYLPH 

       250        260        270        280        290        300 
VTIKSSAGLP YIGKTKGDTT AEALVLADSF IRDLGRAATS ADPEAGVKKT ITDFWYLSCG 

       310        320        330        340        350        360 
LLFPKGERYT QVDWDKKTRN IWSAPYPTHL LLSMVSTPVM NESKLNITNT QTPSLYGFSP 

       370        380        390        400        410        420 
FHGGMDRIMT IIRDSLDNDE DLVMIYADNI YILQDNTWYS IDLEKGEANC TPQHMQAMMY 

       430        440        450        460        470        480 
YLLTRGWTNE DGSPRYNPTW ATFAMNVAPS MVVDSSCLLM NLQLKTYGQG SGNAFTFLNN 

       490        500        510        520        530        540 
HLMSTIVVAE WVKAGKPNPM TKEFMDLEEK TGINFKIERE LKNLRETIVE AVETAPQDGY 

       550        560        570        580        590        600 
LADGSDLPPI RPGKAVELDL LGWSAIYSRQ MEMFVPVLEN ERLIASAAYP KGLENKALAR 

       610        620        630        640        650        660 
KPGAEIAYQI VRYEAIRLVG GWNNPLLETA AKHMSLDKRK RLEVKGIDVT GFLDDWNNMS 

       670        680        690        700        710        720 
EFGGDLEGIT LSEPLTNQTL VDINTPLDSF DPKARPQTPR SPKKTLDEVT TAITSGTYKD 

       730        740        750        760        770        780 
PKSAVWRLLD QRTKLRVSTL RDQALALKPA SSSVDNWAEA TEELAQQQQL LMKANNLLKS 

       790        800        810        820        830        840 
SLTETREALE TIQSDKIIAG KSNPEKNPGT AANPVVGYGE FSEKIPLTPT QKKNAKRREK 


QRRNQ

« Hide

References

[1]"Sequence analysis of infectious pancreatic necrosis virus genome segment B and its encoded VP1 protein: a putative RNA-dependent RNA polymerase lacking the Gly-Asp-Asp motif."
Duncan R., Mason C.L., Nagy E., Leong J.-A., Dobos P.
Virology 181:541-552(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		M58756 Genomic RNA. Translation: AAA46243.1.
PIRRRXSJA. A37946.
RefSeqNP_047197.1. NC_001916.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2YI8X-ray2.30A/B/C/D/E1-790[»]
2YI9X-ray2.20A/B/C/D/E1-790[»]
2YIAX-ray3.02A/B/C/D/E/F/G/H1-790[»]
2YIBX-ray3.80A/B/C1-845[»]
D1-687[»]
3ZEDX-ray2.20A/B/C1-845[»]
ProteinModelPortalP22173.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID956514.

Family and domain databases

InterProIPR007100. RNA-dir_pol_birnavirus.
IPR007098. RNA-dir_pol_mononegavirus.
[Graphical view]
PfamPF04197. Birna_RdRp. 1 hit.
[Graphical view]
PROSITEPS50524. RDRP_DSRNA_BIR. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP22173.

Entry information

Entry nameRDRP_IPNVJ
AccessionPrimary (citable) accession number: P22173
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: August 1, 1991
Last modified: April 3, 2013
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

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