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P22173

- RDRP_IPNVJ

UniProt

P22173 - RDRP_IPNVJ

Protein

RNA-directed RNA polymerase

Gene

VP1

Organism
Infectious pancreatic necrosis virus (strain Jasper) (IPNV)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 75 (01 Oct 2014)
      Sequence version 1 (01 Aug 1991)
      Previous versions | rss
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    Functioni

    RNA-dependent RNA polymerase which is found both free and covalently attached to the genomic RNA. May also contain guanylyl and methyl transferase activities By similarity.By similarity

    Catalytic activityi

    Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).PROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi248 – 2558GTPSequence Analysis

    GO - Molecular functioni

    1. GTP binding Source: UniProtKB-KW
    2. RNA-directed RNA polymerase activity Source: UniProtKB-KW

    GO - Biological processi

    1. RNA-protein covalent cross-linking Source: UniProtKB-KW
    2. viral genome replication Source: InterPro

    Keywords - Molecular functioni

    Nucleotidyltransferase, RNA-directed RNA polymerase, Transferase

    Keywords - Biological processi

    Viral RNA replication

    Keywords - Ligandi

    GTP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    RNA-directed RNA polymerase (EC:2.7.7.48)
    Short name:
    RDRP
    Alternative name(s):
    Protein VP1
    Gene namesi
    Name:VP1
    OrganismiInfectious pancreatic necrosis virus (strain Jasper) (IPNV)
    Taxonomic identifieri11003 [NCBI]
    Taxonomic lineageiVirusesdsRNA virusesBirnaviridaeAquabirnavirus
    Virus hostiOncorhynchus mykiss (Rainbow trout) (Salmo gairdneri) [TaxID: 8022]
    Salmo [TaxID: 8028]
    ProteomesiUP000007248: Genome

    Subcellular locationi

    Virion By similarity
    Note: Minor amounts are incorporated in the virion.By similarity

    GO - Cellular componenti

    1. virion Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Virion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 845845RNA-directed RNA polymerasePRO_0000221963Add
    BLAST

    Post-translational modificationi

    Exists in multiple phosphorylated forms.

    Keywords - PTMi

    Covalent protein-RNA linkage, Phosphoprotein

    Interactioni

    Subunit structurei

    Interacts with VP3 in the cytoplasm.By similarity

    Structurei

    Secondary structure

    1
    845
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi14 – 185
    Beta strandi23 – 253
    Turni28 – 303
    Helixi45 – 5410
    Beta strandi65 – 728
    Helixi73 – 764
    Helixi78 – 803
    Helixi81 – 855
    Beta strandi91 – 10212
    Beta strandi111 – 1133
    Helixi125 – 13713
    Helixi143 – 15614
    Beta strandi157 – 1604
    Helixi164 – 17916
    Beta strandi180 – 1823
    Helixi187 – 1904
    Helixi194 – 20411
    Helixi221 – 2244
    Turni231 – 2333
    Helixi256 – 27823
    Helixi283 – 29311
    Helixi295 – 2984
    Beta strandi299 – 3046
    Beta strandi307 – 3104
    Helixi311 – 3133
    Turni314 – 3163
    Beta strandi320 – 3234
    Helixi326 – 34015
    Turni347 – 3493
    Beta strandi350 – 3545
    Turni361 – 3633
    Helixi364 – 37714
    Beta strandi382 – 3865
    Beta strandi389 – 3946
    Beta strandi397 – 4037
    Helixi406 – 4094
    Helixi412 – 42514
    Beta strandi434 – 4363
    Helixi438 – 4469
    Helixi448 – 4525
    Beta strandi456 – 4605
    Beta strandi462 – 4654
    Helixi476 – 49318
    Helixi502 – 5054
    Helixi507 – 5115
    Beta strandi515 – 5217
    Helixi524 – 53310
    Beta strandi537 – 5393
    Turni540 – 5434
    Beta strandi556 – 5605
    Beta strandi563 – 5686
    Turni569 – 5724
    Beta strandi573 – 5786
    Helixi580 – 5889
    Helixi596 – 5994
    Helixi604 – 61815
    Helixi621 – 6233
    Helixi625 – 64521
    Helixi650 – 6545
    Helixi656 – 6594
    Turni661 – 6655
    Helixi677 – 6848
    Helixi692 – 6943
    Beta strandi699 – 7013
    Helixi706 – 7149
    Turni715 – 7184
    Helixi724 – 7329
    Helixi737 – 7393
    Helixi740 – 75112
    Helixi757 – 77115
    Helixi775 – 7773
    Helixi781 – 79010

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2YI8X-ray2.30A/B/C/D/E1-790[»]
    2YI9X-ray2.20A/B/C/D/E1-790[»]
    2YIAX-ray3.02A/B/C/D/E/F/G/H1-790[»]
    2YIBX-ray3.80A/B/C1-845[»]
    D1-687[»]
    3ZEDX-ray2.20A/B/C1-845[»]
    ProteinModelPortaliP22173.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP22173.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini384 – 588205RdRp catalyticPROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi766 – 7694Poly-Gln

    Sequence similaritiesi

    Contains 1 RdRp catalytic domain.PROSITE-ProRule annotation

    Family and domain databases

    InterProiIPR007100. RNA-dir_pol_birnavirus.
    IPR007098. RNA-dir_pol_mononegavirus.
    [Graphical view]
    PfamiPF04197. Birna_RdRp. 1 hit.
    [Graphical view]
    PROSITEiPS50524. RDRP_DSRNA_BIR. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P22173-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSDIFNSPQN KASILTALMK STTGDVEDVL IPKRFRPAKD PLDSPQAAAQ    50
    FLKDNKYRIL RPRAIPTMVE LETDAALPRL RQMVEDGKLK DTVSVPEGTT 100
    AFYPKYYPFH KPDHDEVGTF GAPDITLLKQ LTFFLLENDF PTGPETLRQV 150
    REAIATLQYG SGSYSGQLNR LLAMKGVATG RNPNKTPKTV GYTNEQLAKL 200
    LEQTLPINTP KHEDPDLRWA PSWLINYTGD LSTDKSYLPH VTIKSSAGLP 250
    YIGKTKGDTT AEALVLADSF IRDLGRAATS ADPEAGVKKT ITDFWYLSCG 300
    LLFPKGERYT QVDWDKKTRN IWSAPYPTHL LLSMVSTPVM NESKLNITNT 350
    QTPSLYGFSP FHGGMDRIMT IIRDSLDNDE DLVMIYADNI YILQDNTWYS 400
    IDLEKGEANC TPQHMQAMMY YLLTRGWTNE DGSPRYNPTW ATFAMNVAPS 450
    MVVDSSCLLM NLQLKTYGQG SGNAFTFLNN HLMSTIVVAE WVKAGKPNPM 500
    TKEFMDLEEK TGINFKIERE LKNLRETIVE AVETAPQDGY LADGSDLPPI 550
    RPGKAVELDL LGWSAIYSRQ MEMFVPVLEN ERLIASAAYP KGLENKALAR 600
    KPGAEIAYQI VRYEAIRLVG GWNNPLLETA AKHMSLDKRK RLEVKGIDVT 650
    GFLDDWNNMS EFGGDLEGIT LSEPLTNQTL VDINTPLDSF DPKARPQTPR 700
    SPKKTLDEVT TAITSGTYKD PKSAVWRLLD QRTKLRVSTL RDQALALKPA 750
    SSSVDNWAEA TEELAQQQQL LMKANNLLKS SLTETREALE TIQSDKIIAG 800
    KSNPEKNPGT AANPVVGYGE FSEKIPLTPT QKKNAKRREK QRRNQ 845
    Length:845
    Mass (Da):94,467
    Last modified:August 1, 1991 - v1
    Checksum:i45C392DF3AAF767A
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M58756 Genomic RNA. Translation: AAA46243.1.
    PIRiA37946. RRXSJA.
    RefSeqiNP_047197.1. NC_001916.1.

    Genome annotation databases

    GeneIDi956514.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M58756 Genomic RNA. Translation: AAA46243.1 .
    PIRi A37946. RRXSJA.
    RefSeqi NP_047197.1. NC_001916.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2YI8 X-ray 2.30 A/B/C/D/E 1-790 [» ]
    2YI9 X-ray 2.20 A/B/C/D/E 1-790 [» ]
    2YIA X-ray 3.02 A/B/C/D/E/F/G/H 1-790 [» ]
    2YIB X-ray 3.80 A/B/C 1-845 [» ]
    D 1-687 [» ]
    3ZED X-ray 2.20 A/B/C 1-845 [» ]
    ProteinModelPortali P22173.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 956514.

    Miscellaneous databases

    EvolutionaryTracei P22173.

    Family and domain databases

    InterProi IPR007100. RNA-dir_pol_birnavirus.
    IPR007098. RNA-dir_pol_mononegavirus.
    [Graphical view ]
    Pfami PF04197. Birna_RdRp. 1 hit.
    [Graphical view ]
    PROSITEi PS50524. RDRP_DSRNA_BIR. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence analysis of infectious pancreatic necrosis virus genome segment B and its encoded VP1 protein: a putative RNA-dependent RNA polymerase lacking the Gly-Asp-Asp motif."
      Duncan R., Mason C.L., Nagy E., Leong J.-A., Dobos P.
      Virology 181:541-552(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

    Entry informationi

    Entry nameiRDRP_IPNVJ
    AccessioniPrimary (citable) accession number: P22173
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1991
    Last sequence update: August 1, 1991
    Last modified: October 1, 2014
    This is version 75 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3