Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Fusion glycoprotein F0

Gene

F

Organism
Bovine respiratory syncytial virus (strain Copenhagen) (BRS)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

During virus entry, induces fusion of viral and cellular membranes leading to delivery of the nucleocapsid into the cytoplasm. The fusogenic activity is inactive untill entry into host cell endosome, where a furin-like protease cleaves off a small peptide between F1 and F2. Interacts directly with heparan sulfate and may participate in virus attachment. Later in infection, proteins F expressed at the plasma membrane of infected cells can mediate fusion with adjacent cells to form syncytia, a cytopathic effect that could lead to tissue necrosis. The fusion protein is also able to trigger p53-dependent apoptosis.By similarity

GO - Biological processi

Keywordsi

Biological processFusion of virus membrane with host cell membrane, Fusion of virus membrane with host membrane, Syncytium formation induced by viral infection, Viral penetration into host cytoplasm, Virus entry into host cell

Names & Taxonomyi

Protein namesi
Recommended name:
Fusion glycoprotein F0
Cleaved into the following 4 chains:
Fusion glycoprotein F2'By similarity
Interchain peptideBy similarity
Gene namesi
Name:F
OrganismiBovine respiratory syncytial virus (strain Copenhagen) (BRS)
Taxonomic identifieri11248 [NCBI]
Taxonomic lineageiVirusesssRNA virusesssRNA negative-strand virusesMononegaviralesPneumoviridaeOrthopneumovirus
Virus hostiBos taurus (Bovine) [TaxID: 9913]

Subcellular locationi

  • Virion membrane By similarity; Single-pass type I membrane protein By similarity
  • Host cell membrane By similarity; Single-pass membrane protein By similarity

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini27 – 529ExtracellularBy similarityAdd BLAST503
Transmembranei530 – 550HelicalSequence analysisAdd BLAST21
Topological domaini551 – 574CytoplasmicBy similarityAdd BLAST24

GO - Cellular componenti

Keywords - Cellular componenti

Host cell membrane, Host membrane, Membrane, Viral envelope protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 25Sequence analysisAdd BLAST25
ChainiPRO_000003922826 – 574Fusion glycoprotein F0Add BLAST549
ChainiPRO_000003922926 – 136Fusion glycoprotein F2Add BLAST111
ChainiPRO_000043265926 – 109Fusion glycoprotein F2'By similarityAdd BLAST84
PeptideiPRO_0000432660110 – 136Interchain peptideBy similarityAdd BLAST27
ChainiPRO_0000039230137 – 574Fusion glycoprotein F1Add BLAST438

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi27N-linked (GlcNAc...) asparagine; by hostSequence analysis1
Disulfide bondi69 ↔ 212Interchain (between F2 and F1 chains)By similarity
Glycosylationi120N-linked (GlcNAc...) asparagine; by hostSequence analysis1
Disulfide bondi358 ↔ 367By similarity
Disulfide bondi382 ↔ 393By similarity
Glycosylationi500N-linked (GlcNAc...) asparagine; by hostSequence analysis1
Lipidationi550S-palmitoyl cysteine; by hostBy similarity1

Post-translational modificationi

The F glycoprotein is synthesized as a F0 inactive precursor that is heavily N-glycosylated and processed by host cell furin in the Golgi to yield the mature F1 and F2 proteins. The cleavage site between F1 and F2 requires the minimal sequence [KR]-X-[KR]-R. During entry a furin-like protease cleaves F2 into F2' and a small peptide, leading to the activation of fusogenic function.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei109 – 110Cleavage; by hostBy similarity2
Sitei136 – 137Cleavage; by hostBy similarity2
Sitei136 – 137Cleavage; by host furinBy similarity2

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond, Glycoprotein, Lipoprotein, Palmitate

Interactioni

Subunit structurei

Homotrimer. Interacts with glycoprotein G. Interacts with host RHOA; this interaction facilitates virus-induced syncytium formation.By similarity

Structurei

3D structure databases

ProteinModelPortaliP22167.
SMRiP22167.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni137 – 157Fusion peptideBy similarityAdd BLAST21

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili155 – 183Sequence analysisAdd BLAST29
Coiled coili491 – 516Sequence analysisAdd BLAST26

Sequence similaritiesi

Keywords - Domaini

Coiled coil, Signal, Transmembrane, Transmembrane helix

Family and domain databases

InterProiView protein in InterPro
IPR000776. Fusion_F0_Paramyxovir.
PfamiView protein in Pfam
PF00523. Fusion_gly. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P22167-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAATAMRMII SIIFISTYMT HITLCQNITE EFYQSTCSAV SRGYLSALRT
60 70 80 90 100
GWYTSVVTIE LSKIQKNVCK STDSKVKLIK QELERYNNAV IELQSLMQNE
110 120 130 140 150
PASFSRAKRG IPELIHYTRN STKRFYGLMG KKRKRRFLGF LLGIGSAIAS
160 170 180 190 200
GVAVSKVLHL EGEVNKIKNA LLSTNKAVVS LSNGVSVLTS KVLDLKNYID
210 220 230 240 250
KELLPKVNNH DCRISNIETV IEFQQKNNRL LEIAREFSVN AGITTPLSTY
260 270 280 290 300
MLTNSELLSL INDMPITNDQ KKLMSSNVQI VRQQSYSIMS VVKEEVIAYV
310 320 330 340 350
VQLPIYGVID TPCWKLHTSP LCTTDNKEGS NICLTRTDRG WYCDNAGSVS
360 370 380 390 400
FFPQAETCKV QSNRVFCDTM NSLTLPTDVN LCNTDIFNTK YDCKIMTSKT
410 420 430 440 450
DISSSVITSI GAIVSCYGKT KCTASNKNRG IIKTFSNGCD YVSNKGVDTV
460 470 480 490 500
SVGNTLYYVN KLEGKALYIK GEPIINYYDP LVFPSDEFDA SIAQVNAKIN
510 520 530 540 550
QSLAFIRRSD ELLHSVDVGK STTNVVITTI IIVIVVVILM LIAVGLLFYC
560 570
KTRSTPIMLG KDQLSGINNL SFSK
Length:574
Mass (Da):63,822
Last modified:August 1, 1991 - v1
Checksum:i6341D60AB80F827B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M58350 mRNA. Translation: AAA42808.1.
PIRiA38492. VGNZBR.

Similar proteinsi

Entry informationi

Entry nameiFUS_BRSVC
AccessioniPrimary (citable) accession number: P22167
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: August 1, 1991
Last modified: May 10, 2017
This is version 92 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families