Reviewed, UniProtKB/Swiss-Prot P22149 (AFT1_YEAST)

Names and origin

Protein names

Recommended name:
    Iron-regulated transcriptional activator AFT1
Alternative name(s):
    Activator of iron transcription protein 1

Gene names

Name:	AFT1
Synonyms:	RCS1
Ordered Locus Names:	YGL071W

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	690 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Probable transcription factor that activates the genes for FRE1, FRE2 and FET3 in response to iron deprivation. Iron could interact directly with AFT1 and inhibits its activity.
Subcellular location	Nucleus Potential.
Miscellaneous	Present with 2730 molecules/cell in log phase SD medium. Ref.7
Sequence caution	The sequence CAA37215.1 differs from that shown. Reason: Frameshift at positions 604 and 644. The sequence CAA54586.1 differs from that shown. Reason: Frameshift at positions 604 and 644.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Ligand	Iron Metal-binding
Molecular function	Activator
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	high-affinity iron ion transport Inferred from mutant phenotype. Source: SGD positive regulation of transcription from RNA polymerase II promoter Inferred from direct assay. Source: SGD transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from direct assay. Source: SGD nucleus Inferred from direct assay. Source: SGD
Molecular function	iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: IntAct sequence-specific DNA binding Inferred from direct assay. Source: SGD transcription factor activity Inferred from direct assay. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
GRX3	Q03835	3	EBI-2332,EBI-22178
GRX4	P32642	1	EBI-2332,EBI-22211

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 690	690	Iron-regulated transcriptional activator AFT1		PRO_0000064487
Amino acid modifications
	Modified residue	193	1	Phosphothreonine Ref.8
Natural variations
	Natural variant	291	1	C → F in allele AFT1-1UP; which is constitutively activated.
Experimental info
	Sequence conflict	8	1	D → H in CAA54586. Ref.2
	Sequence conflict	71	1	I → S in CAA54586. Ref.2
	Sequence conflict	136	1	A → T in CAA54586. Ref.2
	Sequence conflict	236	1	D → N in CAA54586. Ref.2
	Sequence conflict	329	1	S → L in CAA37215. Ref.6
	Sequence conflict	378	1	S → L in CAA37215. Ref.6
	Sequence conflict	416	1	D → G in CAA54586. Ref.2
	Sequence conflict	416	1	D → G in CAA37215. Ref.6
	Sequence conflict	468	1	N → T in CAA37215. Ref.6
	Sequence conflict	507	1	N → S in CAA54586. Ref.2
	Sequence conflict	507	1	N → S in CAA37215. Ref.6
	Sequence conflict	538	1	S → F in CAA54586. Ref.2
	Sequence conflict	538	1	S → L in CAA37215. Ref.6
	Sequence conflict	568	1	S → P in CAA54586. Ref.2
	Sequence conflict	568	1	S → P in CAA37215. Ref.6
	Sequence conflict	579	1	S → T in CAA54586. Ref.2
	Sequence conflict	579	1	S → T in CAA37215. Ref.6
	Sequence conflict	644	1	H → I in CAA54586. Ref.2
	Sequence conflict	644	1	H → I in CAA37215. Ref.6
	Sequence conflict	649	1	H → L in CAA54586. Ref.2
	Sequence conflict	649	1	H → L in CAA37215. Ref.6

Sequences

	Sequence		Length	Mass (Da)
	P22149-1 [UniParc]. Last modified November 1, 1995. Version 2. Checksum: 38641F26B76FCBDD	FASTA	690	77,683
	10 20 30 40 50 60 MEGFNPADIE HASPINSSDS HSSSFVYALP KSASEYVVNH NEGRASASGN PAAVPSPIMT 70 80 90 100 110 120 LNLKSTHSLN IDQHVHTSTS PTETIGHIHH VEKLNQNNLI HLDPVPNFED KSDIKPWLQK 130 140 150 160 170 180 IFYPQGIELV IERSDAFKVV FKCKAAKRGR NARRKRKDKP KGQDHEDEKS KINDDELEYA 190 200 210 220 230 240 SPSNATVTNG PQTSPDQTSS IKPKKKRCVS RFNNCPFRVR ATYSLKRKRW SIVVMDNNHS 250 260 270 280 290 300 HQLKFNPDSE EYKKFKEKLR KDNDVDAIKK FDELEYRTLA NLPIPTATIP CDCGLTNEIQ 310 320 330 340 350 360 SFNVVLPTNS NVTSSASSST VSSISLDSSN ASKRPCLPSV NNTGSINTNN VRKPKSQCKN 370 380 390 400 410 420 KDTLLKRTTM QNFLTTKSRL RKTGTPTSSQ HSSTAFSGYI DDPFNLNEIL PLPASDFKLN 430 440 450 460 470 480 TVTNLNEIDF TNIFTKSPHP HSGSTHPRQV FDQLDDCSSI LFSPLTTNTN NEFEGESDDF 490 500 510 520 530 540 VHSPYLNSEA DFSQILSSAP PVHHDPNETH QENQDIIDRF ANSSQEHNEY ILQYLTHSDA 550 560 570 580 590 600 ANHNNIGVPN NNSHSLNTQH NVSDLGNSLL RQEALVGSSS TKIFDELKFV QNGPHGSQHP 610 620 630 640 650 660 IDFQHVDHRH LSSNEPQVRS HQYGPQQQPP QQLQYHQNQP HDGHNHEQHQ TVQKDMQTHE 670 680 690 SLEIMGNTLL EEFKDIKMVN GELKYVKPED

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"AFT1: a mediator of iron regulated transcriptional control in Saccharomyces cerevisiae." Yamaguchi-Iwai Y., Dancis A., Klausner R.D. EMBO J. 14:1231-1239(1995) [PubMed: 7720713] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The AFT1 transcriptional factor is differentially required for expression of high-affinity iron uptake genes in Saccharomyces cerevisiae." Casas C., Aldea M., Espinet C., Gallego C., Gil R., Herrero E. Yeast 13:621-637(1997) [PubMed: 9200812] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Sequence analysis of 203 kilobases from Saccharomyces cerevisiae chromosome VII." Rieger M., Brueckner M., Schaefer M., Mueller-Auer S. Yeast 13:1077-1090(1997) [PubMed: 9290212] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K. Nature 387:81-84(1997) [PubMed: 9169869] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[6]	"RCS1, a gene involved in controlling cell size in Saccharomyces cerevisiae." Gil R., Zueco J., Sentandreu R., Herrero E. Yeast 7:1-14(1991) [PubMed: 2021081] [Abstract] Cited for: NUCLEOTIDE SEQUENCE OF 283-690. Strain: ATCC 26786 / X2180-1A.
[7]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-193, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z48004 mRNA. Translation: CAA88044.1. X77413 Genomic DNA. Translation: CAA54586.1. Frameshift. Z72593 Genomic DNA. Translation: CAA96775.1. AY723805 Genomic DNA. Translation: AAU09722.1. X53046 Genomic DNA. Translation: CAA37215.1. Frameshift.
PIR	S54775.
RefSeq	NP_011444.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:1351N.
IntAct	P22149. 9 interactions.
STRING	P22149.
Proteomic databases
PeptideAtlas	P22149.
Genome annotation databases
Ensembl	YGL071W; YGL071W; YGL071W; Saccharomyces cerevisiae. [Genome view]
GeneID	852809.
KEGG	sce:YGL071W.
NMPDR	fig|4932.3.peg.2549.
Organism-specific databases
CYGD	YGL071w.
SGD	S000003039. AFT1.
Phylogenomic databases
OrthoDB	EOG94N08G
Gene expression databases
ArrayExpress	P22149.
Genevestigator	P22149.
GermOnline	YGL071W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR014842. AFT. [Graphical view]
Pfam	PF08731. AFT. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	972336.

Entry information

Entry name

AFT1_YEAST

Accession

Primary (citable) accession number: P22149
Secondary accession number(s): Q06993

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1991
Last sequence update:	November 1, 1995
Last modified:	November 24, 2009
This is version 78 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)