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Protein

1,3-beta-glucanosyltransferase GAS1

Gene

GAS1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Splits internally a 1,3-beta-glucan molecule and transfers the newly generated reducing end (the donor) to the non-reducing end of another 1,3-beta-glucan molecule (the acceptor) forming a 1,3-beta linkage, resulting in the elongation of 1,3-beta-glucan chains in the cell wall. Involved in cell wall biosynthesis and morphogenesis.2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei92Donor substrate; via carbonyl oxygenBy similarity1
Binding sitei160Donor substrateBy similarity1
Active sitei161Proton donor1
Binding sitei161Acceptor substrateBy similarity1
Binding sitei202Acceptor substrate; via carbonyl oxygenBy similarity1
Binding sitei207Acceptor substrateBy similarity1
Active sitei262Nucleophile1
Binding sitei294Donor substrateBy similarity1

GO - Molecular functioni

  • 1,3-beta-glucanosyltransferase activity Source: SGD

GO - Biological processi

  • chromatin silencing Source: SGD
  • filamentous growth Source: SGD
  • fungal-type cell wall (1->3)-beta-D-glucan biosynthetic process Source: GO_Central
  • fungal-type cell wall organization Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Cell wall biogenesis/degradation

Enzyme and pathway databases

BioCyciYEAST:G3O-32971-MONOMER.

Protein family/group databases

CAZyiCBM43. Carbohydrate-Binding Module Family 43.
GH72. Glycoside Hydrolase Family 72.

Names & Taxonomyi

Protein namesi
Recommended name:
1,3-beta-glucanosyltransferase GAS1 (EC:2.4.1.-)
Alternative name(s):
Glycolipid-anchored surface protein 1
Glycoprotein GP115
Gene namesi
Name:GAS1
Synonyms:GGP1
Ordered Locus Names:YMR307W
ORF Names:YM9952.09
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

EuPathDBiFungiDB:YMR307W.
SGDiS000004924. GAS1.

Subcellular locationi

GO - Cellular componenti

  • anchored component of external side of plasma membrane Source: GO_Central
  • cellular bud scar Source: SGD
  • ER to Golgi transport vesicle Source: SGD
  • extracellular region Source: UniProtKB-KW
  • fungal-type cell wall Source: SGD
  • membrane raft Source: SGD
  • nuclear periphery Source: SGD
  • plasma membrane Source: SGD
  • primary cell septum Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cell wall, Membrane, Secreted

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi74C → S: Impairs the folding and stability of the protein. 1 Publication1
Mutagenesisi103C → S: Partially impairs the folding and stability of the protein. 1 Publication1
Mutagenesisi161E → Q: Loss of function. 1 Publication1
Mutagenesisi262E → Q: Loss of function. 1 Publication1
Mutagenesisi265C → S: Partially impairs the folding and stability of the protein. 1 Publication1
Mutagenesisi348C → S: Impairs the folding and stability of the protein. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 221 PublicationAdd BLAST22
ChainiPRO_000001047323 – 5281,3-beta-glucanosyltransferase GAS1Add BLAST506
PropeptideiPRO_0000010474529 – 559Removed in mature formAdd BLAST31

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi40N-linked (GlcNAc...)Sequence analysis1
Glycosylationi57N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi74 ↔ 103By similarity
Glycosylationi95N-linked (GlcNAc...)Sequence analysis1
Glycosylationi149N-linked (GlcNAc...)Sequence analysis1
Glycosylationi165N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi216 ↔ 348By similarity
Disulfide bondi234 ↔ 265By similarity
Glycosylationi253N-linked (GlcNAc...)Sequence analysis1
Glycosylationi283N-linked (GlcNAc...)Sequence analysis1
Glycosylationi321N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi370 ↔ 421By similarity
Disulfide bondi372 ↔ 462By similarity
Disulfide bondi379 ↔ 445By similarity
Disulfide bondi398 ↔ 403By similarity
Glycosylationi409N-linked (GlcNAc...)Sequence analysis1
Glycosylationi495N-linked (GlcNAc...)Sequence analysis1
Lipidationi528GPI-anchor amidated asparagine1 Publication1

Post-translational modificationi

Extensively N- and O-glycosylated.1 Publication
The GPI-anchor is attached to the protein in the endoplasmic reticulum and serves to target the protein to the cell surface. There, the glucosamine-inositol phospholipid moiety is cleaved off and the GPI-modified mannoprotein is covalently attached via its lipidless GPI glycan remnant to the 1,6-beta-glucan of the outer cell wall layer.

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein

Proteomic databases

MaxQBiP22146.
PRIDEiP22146.

2D gel databases

COMPLUYEAST-2DPAGEP22146.

Interactioni

Protein-protein interaction databases

BioGridi35487. 502 interactors.
DIPiDIP-4390N.
IntActiP22146. 8 interactors.
MINTiMINT-564340.

Structurei

3D structure databases

ProteinModelPortaliP22146.
SMRiP22146.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni119 – 127Donor substrate bindingBy similarity9

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi438 – 525Ser-richAdd BLAST88

Sequence similaritiesi

Belongs to the glycosyl hydrolase 72 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

GeneTreeiENSGT00390000011003.
HOGENOMiHOG000164982.
InParanoidiP22146.
OMAiGGSKSDC.
OrthoDBiEOG092C1TYR.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR004886. Glucanosyltransferase.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR012946. X8.
[Graphical view]
PfamiPF03198. Glyco_hydro_72. 1 hit.
PF07983. X8. 1 hit.
[Graphical view]
SMARTiSM00768. X8. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P22146-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLFKSLSKLA TAAAFFAGVA TADDVPAIEV VGNKFFYSNN GSQFYIRGVA
60 70 80 90 100
YQADTANETS GSTVNDPLAN YESCSRDIPY LKKLNTNVIR VYAINTTLDH
110 120 130 140 150
SECMKALNDA DIYVIADLAA PATSINRDDP TWTVDLFNSY KTVVDTFANY
160 170 180 190 200
TNVLGFFAGN EVTNNYTNTD ASAFVKAAIR DVRQYISDKN YRKIPVGYSS
210 220 230 240 250
NDDEDTRVKM TDYFACGDDD VKADFYGINM YEWCGKSDFK TSGYADRTAE
260 270 280 290 300
FKNLSIPVFF SEYGCNEVTP RLFTEVEALY GSNMTDVWSG GIVYMYFEET
310 320 330 340 350
NKYGLVSIDG NDVKTLDDFN NYSSEINKIS PTSANTKSYS ATTSDVACPA
360 370 380 390 400
TGKYWSAATE LPPTPNGGLC SCMNAANSCV VSDDVDSDDY ETLFNWICNE
410 420 430 440 450
VDCSGISANG TAGKYGAYSF CTPKEQLSFV MNLYYEKSGG SKSDCSFSGS
460 470 480 490 500
ATLQTATTQA SCSSALKEIG SMGTNSASGS VDLGSGTESS TASSNASGSS
510 520 530 540 550
SKSNSGSSGS SSSSSSSSAS SSSSSKKNAA TNVKANLAQV VFTSIISLSI

AAGVGFALV
Length:559
Mass (Da):59,582
Last modified:February 1, 1996 - v2
Checksum:iD6E39568DCB4C5AE
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti211T → A in CAA37512 (PubMed:1824714).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X53424 Genomic DNA. Translation: CAA37512.1.
X56399 Genomic DNA. Translation: CAA39809.1.
Z49212 Genomic DNA. Translation: CAA89140.1.
BK006946 Genomic DNA. Translation: DAA10208.1.
PIRiS53977. RWBYS1.
RefSeqiNP_014038.1. NM_001182818.1.

Genome annotation databases

EnsemblFungiiYMR307W; YMR307W; YMR307W.
GeneIDi855355.
KEGGisce:YMR307W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X53424 Genomic DNA. Translation: CAA37512.1.
X56399 Genomic DNA. Translation: CAA39809.1.
Z49212 Genomic DNA. Translation: CAA89140.1.
BK006946 Genomic DNA. Translation: DAA10208.1.
PIRiS53977. RWBYS1.
RefSeqiNP_014038.1. NM_001182818.1.

3D structure databases

ProteinModelPortaliP22146.
SMRiP22146.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35487. 502 interactors.
DIPiDIP-4390N.
IntActiP22146. 8 interactors.
MINTiMINT-564340.

Protein family/group databases

CAZyiCBM43. Carbohydrate-Binding Module Family 43.
GH72. Glycoside Hydrolase Family 72.

2D gel databases

COMPLUYEAST-2DPAGEP22146.

Proteomic databases

MaxQBiP22146.
PRIDEiP22146.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYMR307W; YMR307W; YMR307W.
GeneIDi855355.
KEGGisce:YMR307W.

Organism-specific databases

EuPathDBiFungiDB:YMR307W.
SGDiS000004924. GAS1.

Phylogenomic databases

GeneTreeiENSGT00390000011003.
HOGENOMiHOG000164982.
InParanoidiP22146.
OMAiGGSKSDC.
OrthoDBiEOG092C1TYR.

Enzyme and pathway databases

BioCyciYEAST:G3O-32971-MONOMER.

Miscellaneous databases

PROiP22146.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR004886. Glucanosyltransferase.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR012946. X8.
[Graphical view]
PfamiPF03198. Glyco_hydro_72. 1 hit.
PF07983. X8. 1 hit.
[Graphical view]
SMARTiSM00768. X8. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiGAS1_YEAST
AccessioniPrimary (citable) accession number: P22146
Secondary accession number(s): D6W0D4, P23151
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: February 1, 1996
Last modified: November 2, 2016
This is version 152 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 11000 wall-bound molecules/cell in log phase YPD medium.1 Publication

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.