Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P22146 (GAS1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 134. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
1,3-beta-glucanosyltransferase GAS1

EC=2.4.1.-
Alternative name(s):
Glycolipid-anchored surface protein 1
Glycoprotein GP115
Gene names
Name:GAS1
Synonyms:GGP1
Ordered Locus Names:YMR307W
ORF Names:YM9952.09
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length559 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Splits internally a 1,3-beta-glucan molecule and transfers the newly generated reducing end (the donor) to the non-reducing end of another 1,3-beta-glucan molecule (the acceptor) forming a 1,3-beta linkage, resulting in the elongation of 1,3-beta-glucan chains in the cell wall. Involved in cell wall biosynthesis and morphogenesis. Ref.9 Ref.10

Subcellular location

Cell membrane; Lipid-anchorGPI-anchor. Secretedcell wall. Note: Identified as GPI-anchored plasma membrane protein (GPI-PMP) as well as covalently-linked GPI-modified cell wall protein (GPI-CWP) in the outer cell wall layer. Ref.1 Ref.5 Ref.6 Ref.7 Ref.8 Ref.11 Ref.14

Post-translational modification

Extensively N- and O-glycosylated. Ref.14

The GPI-anchor is attached to the protein in the endoplasmic reticulum and serves to target the protein to the cell surface. There, the glucosamine-inositol phospholipid moiety is cleaved off and the GPI-modified mannoprotein is covalently attached via its lipidless GPI glycan remnant to the 1,6-beta-glucan of the outer cell wall layer.

Miscellaneous

Present with 11000 wall-bound molecules/cell in log phase YPD medium. Ref.12

Sequence similarities

Belongs to the glycosyl hydrolase 72 family.

Ontologies

Keywords
   Biological processCell wall biogenesis/degradation
   Cellular componentCell membrane
Cell wall
Membrane
Secreted
   DomainSignal
   Molecular functionTransferase
   PTMDisulfide bond
Glycoprotein
GPI-anchor
Lipoprotein
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processcarbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

chromatin silencing

Inferred from mutant phenotype PubMed 19541632. Source: SGD

filamentous growth

Inferred from mutant phenotype PubMed 15645503. Source: SGD

fungal-type cell wall organization

Inferred from mutant phenotype PubMed 9515908. Source: SGD

   Cellular_componentER to Golgi transport vesicle

Inferred from direct assay PubMed 18462190. Source: SGD

anchored component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

cellular bud scar

Inferred from direct assay PubMed 19793924. Source: SGD

extracellular region

Inferred from electronic annotation. Source: UniProtKB-KW

fungal-type cell wall

Inferred from direct assay Ref.11PubMed 19793924. Source: SGD

membrane raft

Inferred from direct assay PubMed 10716729. Source: SGD

nuclear periphery

Inferred from direct assay PubMed 19541632. Source: SGD

plasma membrane

Inferred from direct assay Ref.1PubMed 19793924. Source: SGD

primary cell septum

Inferred from direct assay PubMed 19793924. Source: SGD

   Molecular_function1,3-beta-glucanosyltransferase activity

Inferred from direct assay Ref.9Ref.10PubMed 17397106. Source: SGD

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2222 Ref.1
Chain23 – 5285061,3-beta-glucanosyltransferase GAS1
PRO_0000010473
Propeptide529 – 55931Removed in mature form
PRO_0000010474

Regions

Region119 – 1279Donor substrate binding By similarity
Compositional bias438 – 52588Ser-rich

Sites

Active site1611Proton donor
Active site2621Nucleophile
Binding site921Donor substrate; via carbonyl oxygen By similarity
Binding site1601Donor substrate By similarity
Binding site1611Acceptor substrate By similarity
Binding site2021Acceptor substrate; via carbonyl oxygen By similarity
Binding site2071Acceptor substrate By similarity
Binding site2941Donor substrate By similarity

Amino acid modifications

Lipidation5281GPI-anchor amidated asparagine Ref.1 Ref.5 Ref.7 Ref.11
Glycosylation401N-linked (GlcNAc...) Potential
Glycosylation571N-linked (GlcNAc...) Potential
Glycosylation951N-linked (GlcNAc...) Potential
Glycosylation1491N-linked (GlcNAc...) Potential
Glycosylation1651N-linked (GlcNAc...) Potential
Glycosylation2531N-linked (GlcNAc...) Potential
Glycosylation2831N-linked (GlcNAc...) Potential
Glycosylation3211N-linked (GlcNAc...) Potential
Glycosylation4091N-linked (GlcNAc...) Potential
Glycosylation4951N-linked (GlcNAc...) Potential
Disulfide bond74 ↔ 103 By similarity
Disulfide bond216 ↔ 348 By similarity
Disulfide bond234 ↔ 265 By similarity
Disulfide bond370 ↔ 421 By similarity
Disulfide bond372 ↔ 462 By similarity
Disulfide bond379 ↔ 445 By similarity
Disulfide bond398 ↔ 403 By similarity

Experimental info

Mutagenesis741C → S: Impairs the folding and stability of the protein. Ref.10
Mutagenesis1031C → S: Partially impairs the folding and stability of the protein. Ref.10
Mutagenesis1611E → Q: Loss of function. Ref.10
Mutagenesis2621E → Q: Loss of function. Ref.10
Mutagenesis2651C → S: Partially impairs the folding and stability of the protein. Ref.10
Mutagenesis3481C → S: Impairs the folding and stability of the protein. Ref.13
Sequence conflict2111T → A in CAA37512. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P22146 [UniParc].

Last modified February 1, 1996. Version 2.
Checksum: D6E39568DCB4C5AE

FASTA55959,582
        10         20         30         40         50         60 
MLFKSLSKLA TAAAFFAGVA TADDVPAIEV VGNKFFYSNN GSQFYIRGVA YQADTANETS 

        70         80         90        100        110        120 
GSTVNDPLAN YESCSRDIPY LKKLNTNVIR VYAINTTLDH SECMKALNDA DIYVIADLAA 

       130        140        150        160        170        180 
PATSINRDDP TWTVDLFNSY KTVVDTFANY TNVLGFFAGN EVTNNYTNTD ASAFVKAAIR 

       190        200        210        220        230        240 
DVRQYISDKN YRKIPVGYSS NDDEDTRVKM TDYFACGDDD VKADFYGINM YEWCGKSDFK 

       250        260        270        280        290        300 
TSGYADRTAE FKNLSIPVFF SEYGCNEVTP RLFTEVEALY GSNMTDVWSG GIVYMYFEET 

       310        320        330        340        350        360 
NKYGLVSIDG NDVKTLDDFN NYSSEINKIS PTSANTKSYS ATTSDVACPA TGKYWSAATE 

       370        380        390        400        410        420 
LPPTPNGGLC SCMNAANSCV VSDDVDSDDY ETLFNWICNE VDCSGISANG TAGKYGAYSF 

       430        440        450        460        470        480 
CTPKEQLSFV MNLYYEKSGG SKSDCSFSGS ATLQTATTQA SCSSALKEIG SMGTNSASGS 

       490        500        510        520        530        540 
VDLGSGTESS TASSNASGSS SKSNSGSSGS SSSSSSSSAS SSSSSKKNAA TNVKANLAQV 

       550 
VFTSIISLSI AAGVGFALV 

« Hide

References

« Hide 'large scale' references
[1]"Determinants for glycophospholipid anchoring of the Saccharomyces cerevisiae GAS1 protein to the plasma membrane."
Nuoffer C., Jenoe P., Conzelmann A., Riezman H.
Mol. Cell. Biol. 11:27-37(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 23-39; 237-240; 435-437 AND 527-528, SUBCELLULAR LOCATION, GPI-ANCHOR AT ASN-528.
[2]"Isolation and deduced amino acid sequence of the gene encoding gp115, a yeast glycophospholipid-anchored protein containing a serine-rich region."
Vai M., Gatti E., Lacana E., Popolo L., Alberghina L.
J. Biol. Chem. 266:12242-12248(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: DBY939.
[3]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII."
Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. expand/collapse author list , Skelton J., Walsh S.V., Whitehead S., Barrell B.G.
Nature 387:90-93(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"A major 125-kd membrane glycoprotein of Saccharomyces cerevisiae is attached to the lipid bilayer through an inositol-containing phospholipid."
Conzelmann A., Riezman H., Desponds C., Bron C.
EMBO J. 7:2233-2240(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, GPI-ANCHOR.
[6]"Immunochemical characterization of gp115, a yeast glycoprotein modulated by the cell cycle."
Popolo L., Grandori R., Vai M., Lacana E., Alberghina L.
Eur. J. Cell Biol. 47:173-180(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[7]"The cell cycle modulated glycoprotein GP115 is one of the major yeast proteins containing glycosylphosphatidylinositol."
Vai M., Popolo L., Grandori R., Lacana E., Alberghina L.
Biochim. Biophys. Acta 1038:277-285(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: GPI-ANCHOR.
[8]"A constitutive role for GPI anchors in Saccharomyces cerevisiae: cell wall targeting."
De Sampaio G., Bourdineaud J.-P., Lauquin G.J.-M.
Mol. Microbiol. 34:247-256(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[9]"Glycosylphosphatidylinositol-anchored glucanosyltransferases play an active role in the biosynthesis of the fungal cell wall."
Mouyna I., Fontaine T., Vai M., Monod M., Fonzi W.A., Diaquin M., Popolo L., Hartland R.P., Latge J.-P.
J. Biol. Chem. 275:14882-14889(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[10]"Characterization of recombinant forms of the yeast Gas1 protein and identification of residues essential for glucanosyltransferase activity and folding."
Carotti C., Ragni E., Palomares O., Fontaine T., Tedeschi G., Rodriguez R., Latge J.-P., Vai M., Popolo L.
Eur. J. Biochem. 271:3635-3645(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, MUTAGENESIS OF CYS-74; CYS-103; GLU-161; GLU-262 AND CYS-265.
[11]"Comprehensive proteomic analysis of Saccharomyces cerevisiae cell walls: identification of proteins covalently attached via glycosylphosphatidylinositol remnants or mild alkali-sensitive linkages."
Yin Q.Y., de Groot P.W.J., Dekker H.L., de Jong L., Klis F.M., de Koster C.G.
J. Biol. Chem. 280:20894-20901(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, IDENTIFICATION BY MASS SPECTROMETRY, GPI-ANCHOR.
[12]"Mass spectrometric quantitation of covalently bound cell wall proteins in Saccharomyces cerevisiae."
Yin Q.Y., de Groot P.W.J., de Jong L., Klis F.M., de Koster C.G.
FEMS Yeast Res. 7:887-896(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION, IDENTIFICATION BY MASS SPECTROMETRY.
[13]"Disulfide bond structure and domain organization of yeast beta(1,3)-glucanosyltransferases involved in cell wall biogenesis."
Popolo L., Ragni E., Carotti C., Palomares O., Aardema R., Back J.W., Dekker H.L., de Koning L.J., de Jong L., de Koster C.G.
J. Biol. Chem. 283:18553-18565(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: MUTAGENESIS OF CYS-348.
[14]"An engineered Saccharomyces cerevisiae strain binds the broadly neutralizing human immunodeficiency virus type 1 antibody 2G12 and elicits mannose-specific gp120-binding antibodies."
Luallen R.J., Lin J., Fu H., Cai K.K., Agrawal C., Mboudjeka I., Lee F.-H., Montefiori D., Smith D.F., Doms R.W., Geng Y.
J. Virol. 82:6447-6457(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, GLYCOSYLATION, IDENTIFICATION BY MASS SPECTROMETRY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X53424 Genomic DNA. Translation: CAA37512.1.
X56399 Genomic DNA. Translation: CAA39809.1.
Z49212 Genomic DNA. Translation: CAA89140.1.
BK006946 Genomic DNA. Translation: DAA10208.1.
PIRRWBYS1. S53977.
RefSeqNP_014038.1. NM_001182818.1.

3D structure databases

ProteinModelPortalP22146.
SMRP22146. Positions 23-475.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid35487. 496 interactions.
DIPDIP-4390N.
IntActP22146. 8 interactions.
MINTMINT-564340.
STRING4932.YMR307W.

Protein family/group databases

CAZyCBM43. Carbohydrate-Binding Module Family 43.
GH72. Glycoside Hydrolase Family 72.

2D gel databases

COMPLUYEAST-2DPAGEP22146.

Proteomic databases

MaxQBP22146.
PaxDbP22146.
PeptideAtlasP22146.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYMR307W; YMR307W; YMR307W.
GeneID855355.
KEGGsce:YMR307W.

Organism-specific databases

CYGDYMR307w.
SGDS000004924. GAS1.

Phylogenomic databases

eggNOGNOG73259.
GeneTreeENSGT00390000011003.
HOGENOMHOG000164982.
OMAGGSKSDC.
OrthoDBEOG7K3TW7.

Enzyme and pathway databases

BioCycYEAST:G3O-32971-MONOMER.

Gene expression databases

GenevestigatorP22146.

Family and domain databases

Gene3D3.20.20.80. 1 hit.
InterProIPR004886. Glucanosyltransferase.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR012946. X8.
[Graphical view]
PfamPF03198. Glyco_hydro_72. 1 hit.
PF07983. X8. 1 hit.
[Graphical view]
SMARTSM00768. X8. 1 hit.
[Graphical view]
SUPFAMSSF51445. SSF51445. 1 hit.
ProtoNetSearch...

Other

NextBio979115.

Entry information

Entry nameGAS1_YEAST
AccessionPrimary (citable) accession number: P22146
Secondary accession number(s): D6W0D4, P23151
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: February 1, 1996
Last modified: July 9, 2014
This is version 134 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XIII

Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries