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P22143 (CYAH_MYRVE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 58. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order

Names and origin

Protein namesRecommended name:
Cyanamide hydratase

EC=4.2.1.69
Alternative name(s):
Urea hydro-lyase
Gene names
Name:CAH
OrganismMyrothecium verrucaria
Taxonomic identifier5532 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesHypocreomycetidaeHypocrealesmitosporic HypocrealesMyrothecium

Protein attributes

Sequence length244 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

When used as herbicide in agriculture, cyanamide can be transformed, after sowing, in soil fertilizing ammonia by the combined action of M.verrucaria cyanamide hydratase and urease.

Catalytic activity

Urea = cyanamide + H2O.

Cofactor

Zinc.

Subunit structure

Homohexamer.

Miscellaneous

This enzyme is highly specific for cyanamide.

Ontologies

Keywords
   LigandZinc
   Molecular functionLyase
   Technical termDirect protein sequencing
Gene Ontology (GO)
   Biological_processcyanamide metabolic process

Inferred from direct assay Ref.1. Source: UniProtKB

   Molecular_functioncyanamide hydratase activity

Inferred from direct assay Ref.1. Source: UniProtKB

phosphoric diester hydrolase activity

Inferred from electronic annotation. Source: InterPro

zinc ion binding

Inferred from direct assay Ref.1. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 244244Cyanamide hydratase
PRO_0000079747

Sequences

Sequence LengthMass (Da)Tools
P22143 [UniParc].

Last modified August 1, 1991. Version 1.
Checksum: 880FA11F30E31CE2

FASTA24426,966
        10         20         30         40         50         60 
MSSSEVKANG WTAVPVSAKA IVDSLGKLGD VSSYSVEDIA FPAADKLVAE AQAFVKARLS 

        70         80         90        100        110        120 
PETYNHSMRV FYWGTVIARR LLPEQAKDLS PSTWALTCLL HDVGTAEAYF TSTRMSFDIY 

       130        140        150        160        170        180 
GGIKAMEVLK VLGSSTDQAE AVAEAIIRHE DVGVDGNITF LGQLIQLATL YDNVGAYDGI 

       190        200        210        220        230        240 
DDFGSWVDDT TRNSINTAFP RHGWCSWFAC TVRKEESNKP WCHTTHIPQF DKQMEANTLM 


KPWE 

« Hide

References

[1]"Isolation and properties of a nitrile hydratase from the soil fungus Myrothecium verrucaria that is highly specific for the fertilizer cyanamide and cloning of its gene."
Maier-Greiner U.M., Obermaier-Skrobranek B.M.M., Estermaier L.M., Kammerloher W., Freund C., Wuelfing C., Burkert U.I., Matern D.H., Breuer M., Eulitz M., Kuefrevioglu O.I., Hartmann G.R.
Proc. Natl. Acad. Sci. U.S.A. 88:4260-4264(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
Strain: DSM 2087.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M59078 Genomic DNA. Translation: AAA33429.1.
PIRA39365.

3D structure databases

ProteinModelPortalP22143.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D1.10.3210.10. 1 hit.
InterProIPR017771. Cyanamide_hydratase_HD.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
[Graphical view]
PfamPF01966. HD. 1 hit.
[Graphical view]
SMARTSM00471. HDc. 1 hit.
[Graphical view]
TIGRFAMsTIGR03401. cyanamide_fam. 1 hit.
ProtoNetSearch...

Entry information

Entry nameCYAH_MYRVE
AccessionPrimary (citable) accession number: P22143
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: August 1, 1991
Last modified: April 3, 2013
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program