P22143 (CYAH_MYRVE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cyanamide hydratase
EC=4.2.1.69

Alternative name(s):
Urea hydro-lyase

Gene names

Name:

CAH

Organism

Myrothecium verrucaria

Taxonomic identifier

5532 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Pezizomycotina › leotiomyceta › sordariomyceta › Sordariomycetes › Hypocreomycetidae › Hypocreales › mitosporic Hypocreales › Myrothecium

Protein attributes

Sequence length	244 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	When used as herbicide in agriculture, cyanamide can be transformed, after sowing, in soil fertilizing ammonia by the combined action of M.verrucaria cyanamide hydratase and urease.
Catalytic activity	Urea = cyanamide + H2O.
Cofactor	Zinc.
Subunit structure	Homohexamer.
Miscellaneous	This enzyme is highly specific for cyanamide.

Ontologies

Keywords
Ligand	Zinc
Molecular function	Lyase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological_process	cyanamide metabolic process Inferred from direct assay Ref.1. Source: UniProtKB
Molecular_function	cyanamide hydratase activity Inferred from direct assay Ref.1. Source: UniProtKB phosphoric diester hydrolase activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from direct assay Ref.1. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 244	244	Cyanamide hydratase		PRO_0000079747

Sequences

	Sequence		Length	Mass (Da)
	P22143 [UniParc]. Last modified August 1, 1991. Version 1. Checksum: 880FA11F30E31CE2	FASTA	244	26,966
	10 20 30 40 50 60 MSSSEVKANG WTAVPVSAKA IVDSLGKLGD VSSYSVEDIA FPAADKLVAE AQAFVKARLS 70 80 90 100 110 120 PETYNHSMRV FYWGTVIARR LLPEQAKDLS PSTWALTCLL HDVGTAEAYF TSTRMSFDIY 130 140 150 160 170 180 GGIKAMEVLK VLGSSTDQAE AVAEAIIRHE DVGVDGNITF LGQLIQLATL YDNVGAYDGI 190 200 210 220 230 240 DDFGSWVDDT TRNSINTAFP RHGWCSWFAC TVRKEESNKP WCHTTHIPQF DKQMEANTLM KPWE

References

[1]

"Isolation and properties of a nitrile hydratase from the soil fungus Myrothecium verrucaria that is highly specific for the fertilizer cyanamide and cloning of its gene." Maier-Greiner U.M., Obermaier-Skrobranek B.M.M., Estermaier L.M., Kammerloher W., Freund C., Wuelfing C., Burkert U.I., Matern D.H., Breuer M., Eulitz M., Kuefrevioglu O.I., Hartmann G.R. Proc. Natl. Acad. Sci. U.S.A. 88:4260-4264(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE. Strain: DSM 2087.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M59078 Genomic DNA. Translation: AAA33429.1.
PIR	A39365.
3D structure databases
ProteinModelPortal	P22143.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	1.10.3210.10. 1 hit.
InterPro	IPR017771. Cyanamide_hydratase_HD. IPR003607. HD/PDEase_dom. IPR006674. HD_domain. [Graphical view]
Pfam	PF01966. HD. 1 hit. [Graphical view]
SMART	SM00471. HDc. 1 hit. [Graphical view]
TIGRFAMs	TIGR03401. cyanamide_fam. 1 hit.
ProtoNet	Search...

Entry information

Entry name

CYAH_MYRVE

Accession

Primary (citable) accession number: P22143

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1991
Last sequence update:	August 1, 1991
Last modified:	April 3, 2013
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program