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P22143

- CYAH_MYRVE

UniProt

P22143 - CYAH_MYRVE

Protein

Cyanamide hydratase

Gene

CAH

Organism
Myrothecium verrucaria
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 58 (03 Apr 2013)
      Sequence version 1 (01 Aug 1991)
      Previous versions | rss
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    Functioni

    When used as herbicide in agriculture, cyanamide can be transformed, after sowing, in soil fertilizing ammonia by the combined action of M.verrucaria cyanamide hydratase and urease.

    Catalytic activityi

    Urea = cyanamide + H2O.

    Cofactori

    Zinc.

    GO - Molecular functioni

    1. cyanamide hydratase activity Source: UniProtKB
    2. phosphoric diester hydrolase activity Source: InterPro
    3. zinc ion binding Source: UniProtKB

    GO - Biological processi

    1. cyanamide metabolic process Source: UniProtKB

    Keywords - Molecular functioni

    Lyase

    Keywords - Ligandi

    Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cyanamide hydratase (EC:4.2.1.69)
    Alternative name(s):
    Urea hydro-lyase
    Gene namesi
    Name:CAH
    OrganismiMyrothecium verrucaria
    Taxonomic identifieri5532 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesHypocreomycetidaeHypocrealesmitosporic HypocrealesMyrothecium

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 244244Cyanamide hydratasePRO_0000079747Add
    BLAST

    Interactioni

    Subunit structurei

    Homohexamer.

    Structurei

    3D structure databases

    ProteinModelPortaliP22143.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Family and domain databases

    Gene3Di1.10.3210.10. 1 hit.
    InterProiIPR017771. Cyanamide_hydratase_HD.
    IPR003607. HD/PDEase_dom.
    IPR006674. HD_domain.
    [Graphical view]
    PfamiPF01966. HD. 1 hit.
    [Graphical view]
    SMARTiSM00471. HDc. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR03401. cyanamide_fam. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P22143-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSSSEVKANG WTAVPVSAKA IVDSLGKLGD VSSYSVEDIA FPAADKLVAE    50
    AQAFVKARLS PETYNHSMRV FYWGTVIARR LLPEQAKDLS PSTWALTCLL 100
    HDVGTAEAYF TSTRMSFDIY GGIKAMEVLK VLGSSTDQAE AVAEAIIRHE 150
    DVGVDGNITF LGQLIQLATL YDNVGAYDGI DDFGSWVDDT TRNSINTAFP 200
    RHGWCSWFAC TVRKEESNKP WCHTTHIPQF DKQMEANTLM KPWE 244
    Length:244
    Mass (Da):26,966
    Last modified:August 1, 1991 - v1
    Checksum:i880FA11F30E31CE2
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M59078 Genomic DNA. Translation: AAA33429.1.
    PIRiA39365.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M59078 Genomic DNA. Translation: AAA33429.1 .
    PIRi A39365.

    3D structure databases

    ProteinModelPortali P22143.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 1.10.3210.10. 1 hit.
    InterProi IPR017771. Cyanamide_hydratase_HD.
    IPR003607. HD/PDEase_dom.
    IPR006674. HD_domain.
    [Graphical view ]
    Pfami PF01966. HD. 1 hit.
    [Graphical view ]
    SMARTi SM00471. HDc. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR03401. cyanamide_fam. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Isolation and properties of a nitrile hydratase from the soil fungus Myrothecium verrucaria that is highly specific for the fertilizer cyanamide and cloning of its gene."
      Maier-Greiner U.M., Obermaier-Skrobranek B.M.M., Estermaier L.M., Kammerloher W., Freund C., Wuelfing C., Burkert U.I., Matern D.H., Breuer M., Eulitz M., Kuefrevioglu O.I., Hartmann G.R.
      Proc. Natl. Acad. Sci. U.S.A. 88:4260-4264(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
      Strain: DSM 2087.

    Entry informationi

    Entry nameiCYAH_MYRVE
    AccessioniPrimary (citable) accession number: P22143
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1991
    Last sequence update: August 1, 1991
    Last modified: April 3, 2013
    This is version 58 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    This enzyme is highly specific for cyanamide.

    Keywords - Technical termi

    Direct protein sequencing

    External Data

    Dasty 3