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Protein

Cyanamide hydratase

Gene

CAH

Organism
Myrothecium verrucaria
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Cyanamide hydratase involved in the detoxification and/or utilization of cyanamide, a toxic nitrile compound distributed widely in the environment.4 Publications

Catalytic activityi

Urea = cyanamide + H2O.3 Publications

Cofactori

Zn2+1 Publication

Enzyme regulationi

Compounds that react with thiol groups, such as Ag+, p-chloromercuribenzoate, and iodoacetamide show a strong inhibitory effect on cyanamide hydratase activity. The enzymatic activity is also sensitive to chelating agents such as EDTA or o-phenanthroline, as well as to reagents containing structural elements of cyanamide, such as urea, dicyandiamide, azide, hydroxylamine, thiocyanate, or cyanate.1 Publication

Kineticsi

  1. KM=27 mM for cyanamide.1 Publication

    pH dependencei

    Optimum pH is 7.7.1 Publication

    Temperature dependencei

    Optimum temperature is 55 degrees Celsius.1 Publication

    GO - Molecular functioni

    • cyanamide hydratase activity Source: UniProtKB
    • zinc ion binding Source: UniProtKB

    GO - Biological processi

    • cyanamide metabolic process Source: UniProtKB
    Complete GO annotation...

    Keywords - Molecular functioni

    Lyase

    Keywords - Ligandi

    Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cyanamide hydratase1 Publication (EC:4.2.1.693 Publications)
    Alternative name(s):
    Urea hydro-lyaseCurated
    Gene namesi
    Name:CAH1 Publication
    OrganismiMyrothecium verrucaria
    Taxonomic identifieri5532 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesHypocreomycetidaeHypocrealesmitosporic HypocrealesMyrothecium

    Pathology & Biotechi

    Biotechnological usei

    Transgenic plants expressing CAH acquire resistance to toxic cyanamide (PubMed:16173468). Moreover, due to its innate ability to convert cyanamide to urea and the broad-spectrum antimicrobial activity of cyanamide, the CAH gene can be used to facilitate plant growth promotion and biocontrol of phytopathogens (Ref. 2, PubMed:18182434, PubMed:17899261).4 Publications

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 244244Cyanamide hydratasePRO_0000079747Add
    BLAST

    Interactioni

    Subunit structurei

    Homohexamer.1 Publication

    Structurei

    3D structure databases

    ProteinModelPortaliP22143.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini63 – 15492HDSequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Belongs to the cyanamide dehydrase family.Curated
    Contains 1 HD domain.Sequence Analysis

    Family and domain databases

    Gene3Di1.10.3210.10. 1 hit.
    InterProiIPR017771. Cyanamide_hydratase_HD.
    IPR003607. HD/PDEase_dom.
    IPR006674. HD_domain.
    [Graphical view]
    PfamiPF01966. HD. 1 hit.
    [Graphical view]
    SMARTiSM00471. HDc. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR03401. cyanamide_fam. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P22143-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MSSSEVKANG WTAVPVSAKA IVDSLGKLGD VSSYSVEDIA FPAADKLVAE
    60 70 80 90 100
    AQAFVKARLS PETYNHSMRV FYWGTVIARR LLPEQAKDLS PSTWALTCLL
    110 120 130 140 150
    HDVGTAEAYF TSTRMSFDIY GGIKAMEVLK VLGSSTDQAE AVAEAIIRHE
    160 170 180 190 200
    DVGVDGNITF LGQLIQLATL YDNVGAYDGI DDFGSWVDDT TRNSINTAFP
    210 220 230 240
    RHGWCSWFAC TVRKEESNKP WCHTTHIPQF DKQMEANTLM KPWE
    Length:244
    Mass (Da):26,966
    Last modified:August 1, 1991 - v1
    Checksum:i880FA11F30E31CE2
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    M59078 Genomic DNA. Translation: AAA33429.1.
    PIRiA39365.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    M59078 Genomic DNA. Translation: AAA33429.1.
    PIRiA39365.

    3D structure databases

    ProteinModelPortaliP22143.
    ModBaseiSearch...
    MobiDBiSearch...

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Family and domain databases

    Gene3Di1.10.3210.10. 1 hit.
    InterProiIPR017771. Cyanamide_hydratase_HD.
    IPR003607. HD/PDEase_dom.
    IPR006674. HD_domain.
    [Graphical view]
    PfamiPF01966. HD. 1 hit.
    [Graphical view]
    SMARTiSM00471. HDc. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR03401. cyanamide_fam. 1 hit.
    ProtoNetiSearch...

    Publicationsi

    1. "Isolation and properties of a nitrile hydratase from the soil fungus Myrothecium verrucaria that is highly specific for the fertilizer cyanamide and cloning of its gene."
      Maier-Greiner U.M., Obermaier-Skrobranek B.M.M., Estermaier L.M., Kammerloher W., Freund C., Wuelfing C., Burkert U.I., Matern D.H., Breuer M., Eulitz M., Kuefrevioglu O.I., Hartmann G.R.
      Proc. Natl. Acad. Sci. U.S.A. 88:4260-4264(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE, SUBUNIT, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, COFACTOR, ENZYME REGULATION, FUNCTION.
      Strain: DSM 2087.
    2. "Herbicide resistance in transgenic plants through degradation of the phytotoxin to urea."
      Maier-Greiner U.H., Klaus C.B.A., Estermaier L.M., Hartman G.R.
      Angew. Chem. Int. Ed. Engl. 30:1314-1315(1991)
      Cited for: FUNCTION, BIOTECHNOLOGY.
    3. "Expression of a fungal cyanamide hydratase in transgenic soybean detoxifies cyanamide in tissue culture and in planta to provide cyanamide resistance."
      Zhang X.H., Zhong W.Q., Widholm J.M.
      J. Plant Physiol. 162:1064-1073(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, CATALYTIC ACTIVITY, BIOTECHNOLOGY.
    4. "Effect of the expression of cyanamide hydratase on metabolites in cyanamide-treated soybean plants kept in the light or dark."
      Ulanov A., Widholm J.M.
      J. Exp. Bot. 58:4319-4332(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, CATALYTIC ACTIVITY, BIOTECHNOLOGY.
    5. "Site-directed mutagenesis, heterologous expression of cyanamide hydratase gene and antimicrobial activity of cyanamide."
      Kirubakaran S.I., Sakthivel N.
      Curr. Microbiol. 56:42-47(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: BIOTECHNOLOGY.

    Entry informationi

    Entry nameiCYAH_MYRVE
    AccessioniPrimary (citable) accession number: P22143
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1991
    Last sequence update: August 1, 1991
    Last modified: June 24, 2015
    This is version 61 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    This enzyme is highly specific for cyanamide.1 Publication

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.