Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P22142 (NDUS2_NEUCR) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 116. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
NADH-ubiquinone oxidoreductase 49 kDa subunit, mitochondrial

EC=1.6.5.3
EC=1.6.99.3
Alternative name(s):
Complex I-49kD
Short name=CI-49kD
Gene names
Name:nuo-49
ORF Names:NCU02534
OrganismNeurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) [Reference proteome]
Taxonomic identifier367110 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesSordariomycetidaeSordarialesSordariaceaeNeurospora

Protein attributes

Sequence length478 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. HAMAP-Rule MF_01358

Catalytic activity

NADH + ubiquinone + 5 H+(In) = NAD+ + ubiquinol + 4 H+(Out). HAMAP-Rule MF_01358

NADH + acceptor = NAD+ + reduced acceptor. HAMAP-Rule MF_01358

Cofactor

Binds 1 4Fe-4S cluster.

Subunit structure

Complex I is composed of about 40 different subunits.

Subcellular location

Mitochondrion inner membrane; Peripheral membrane protein; Matrix side HAMAP-Rule MF_01358.

Sequence similarities

Belongs to the complex I 49 kDa subunit family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 4242Mitochondrion Ref.1
Chain43 – 478436NADH-ubiquinone oxidoreductase 49 kDa subunit, mitochondrial HAMAP-Rule MF_01358
PRO_0000019984

Sites

Metal binding3411Iron-sulfur (4Fe-4S) Potential
Metal binding3471Iron-sulfur (4Fe-4S) Potential
Metal binding3621Iron-sulfur (4Fe-4S) Potential

Experimental info

Sequence conflict4171A → G in CAA38368. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P22142 [UniParc].

Last modified December 4, 2007. Version 2.
Checksum: 159C35BE3DF9D916

FASTA47854,019
        10         20         30         40         50         60 
MATTLFRLAG RNAKRHCMRQ STTIAHNLNS TRAFSASALR RYAEPSYEGQ GTRLVPTGDD 

        70         80         90        100        110        120 
FAPNNDLYGL EALKADGAPR VPPQDHILAR KVRHYTVNFG PQHPAAHGVL RLILELKGEE 

       130        140        150        160        170        180 
IVRADPHVGL LHRGTEKLCE YRTYLQALPY FDRLDYVSMM TNEQCFALAV EKLLNVEIPE 

       190        200        210        220        230        240 
RAKWIRTMFA EITRILNHLM SVLSHAMDVG ALTPFLWGFE EREKLMEFYE RVSGARLHAA 

       250        260        270        280        290        300 
YVRPGGVHQD IPLGLLDDIY MWATQFGDRI DETEEMLTDN RIWIDRLRGI GVVSAADALN 

       310        320        330        340        350        360 
LSFTGVMLRG SGVPWDIRKS QPYDAYDQVE FDVPVGINGD CYDRYLCRME EFRQSLRIIH 

       370        380        390        400        410        420 
QCLNKMPAGP VRVEDYKISP PPRSAMKENM EALIHHFLLY TKGYAVPPGD TYSAIEAPKG 

       430        440        450        460        470 
EMGVYVVSDG SERPYRVHIR APGFAHLGGF DHLSRGHMLA DAVAVIGTMD LVFGEVDR 

« Hide

References

« Hide 'large scale' references
[1]"The 49 K subunit of NADH: ubiquinone reductase (complex I) from Neurospora crassa mitochondria: primary structure of the gene and the protein."
Preis D., van der Pas J.C., Nehls U., Roehlen D.-A., Sackmann U., Jahnke U., Weiss H.
Curr. Genet. 18:59-64(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 43-62.
Strain: 74-ORS-6a / FGSC 4200.
[2]"The genome sequence of the filamentous fungus Neurospora crassa."
Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D., Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B., Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M., Qui D. expand/collapse author list , Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M., Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U., Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D., Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S., Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D., Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S., Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A., DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R., Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R., Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I., Sachs M.S., Lander E.S., Nusbaum C., Birren B.W.
Nature 422:859-868(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X54508 Genomic DNA. Translation: CAA38368.1.
CM002236 Genomic DNA. Translation: EAA36429.2.
PIRS13801.
RefSeqXP_965665.2. XM_960572.2.
UniGeneNcr.16312.

3D structure databases

ProteinModelPortalP22142.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING5141.NCU02534.1.

Protein family/group databases

TCDB3.D.1.6.2. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiEFNCRT00000002181; EFNCRP00000002181; EFNCRG00000002178.
GeneID3881799.
KEGGncr:NCU02534.

Phylogenomic databases

eggNOGCOG0649.
HOGENOMHOG000228264.
KOK03935.
OrthoDBEOG7B31WZ.

Family and domain databases

HAMAPMF_01358. NDH1_NuoD.
InterProIPR001135. NADH_Q_OxRdtase_suD.
IPR014029. NADH_UbQ_OxRdtase_49kDa_CS.
IPR022885. NDH1_su_D/H.
[Graphical view]
PfamPF00346. Complex1_49kDa. 1 hit.
[Graphical view]
TIGRFAMsTIGR01962. NuoD. 1 hit.
PROSITEPS00535. COMPLEX1_49K. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNDUS2_NEUCR
AccessionPrimary (citable) accession number: P22142
Secondary accession number(s): Q7RVM8
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: December 4, 2007
Last modified: February 19, 2014
This is version 116 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families