NADH-ubiquinone oxidoreductase 49 kDa subunit, mitochondrial precursor - Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)

Contribute

Send feedback

Read comments (?) or add your own

P22142 (NDUS2_NEUCR) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 110. History...

Clusters with 100%, 90%, 50% identity |

Documents (1) |

Third-party data

text xml rdf/xml gff fasta

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
NADH-ubiquinone oxidoreductase 49 kDa subunit, mitochondrial
EC=1.6.5.3
EC=1.6.99.3

Alternative name(s):
Complex I-49kD
Short name=CI-49kD

Gene names

Name:	nuo-49
ORF Names:	NCU02534

Organism

Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) [Reference proteome]

Taxonomic identifier

367110 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Sordariomycetes › Sordariomycetidae › Sordariales › Sordariaceae › Neurospora ›

Protein attributes

Sequence length	478 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
Catalytic activity	NADH + ubiquinone = NAD⁺ + ubiquinol. NADH + acceptor = NAD⁺ + reduced acceptor.
Cofactor	Binds 1 4Fe-4S cluster.
Subunit structure	Complex I is composed of about 40 different subunits.
Subcellular location	Mitochondrion inner membrane; Peripheral membrane protein; Matrix side.
Sequence similarities	Belongs to the complex I 49 kDa subunit family.

Ontologies

Keywords
Biological process	Electron transport Respiratory chain Transport
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Domain	Transit peptide
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding NAD Ubiquinone
Molecular function	Oxidoreductase
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	mitochondrial inner membrane Inferred from electronic annotation. Source: UniProtKB-SubCell respiratory chain Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW NAD binding Inferred from electronic annotation. Source: InterPro NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW quinone binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Transit peptide

1 – 42

Mitochondrion Ref.1

Chain

43 – 478

436

NADH-ubiquinone oxidoreductase 49 kDa subunit, mitochondrial

PRO_0000019984

Sites

Metal binding

341

Iron-sulfur (4Fe-4S) Potential

Metal binding

347

Iron-sulfur (4Fe-4S) Potential

Metal binding

362

Iron-sulfur (4Fe-4S) Potential

Experimental info

Sequence conflict

417

A → G in CAA38368. Ref.1

Sequences

Sequence

Length

Mass (Da)

Tools

P22142 [UniParc].

Last modified December 4, 2007. Version 2.
Checksum: 159C35BE3DF9D916
Show »

FASTA

478

54,019

        10         20         30         40         50         60 
MATTLFRLAG RNAKRHCMRQ STTIAHNLNS TRAFSASALR RYAEPSYEGQ GTRLVPTGDD 

        70         80         90        100        110        120 
FAPNNDLYGL EALKADGAPR VPPQDHILAR KVRHYTVNFG PQHPAAHGVL RLILELKGEE 

       130        140        150        160        170        180 
IVRADPHVGL LHRGTEKLCE YRTYLQALPY FDRLDYVSMM TNEQCFALAV EKLLNVEIPE 

       190        200        210        220        230        240 
RAKWIRTMFA EITRILNHLM SVLSHAMDVG ALTPFLWGFE EREKLMEFYE RVSGARLHAA 

       250        260        270        280        290        300 
YVRPGGVHQD IPLGLLDDIY MWATQFGDRI DETEEMLTDN RIWIDRLRGI GVVSAADALN 

       310        320        330        340        350        360 
LSFTGVMLRG SGVPWDIRKS QPYDAYDQVE FDVPVGINGD CYDRYLCRME EFRQSLRIIH 

       370        380        390        400        410        420 
QCLNKMPAGP VRVEDYKISP PPRSAMKENM EALIHHFLLY TKGYAVPPGD TYSAIEAPKG 

       430        440        450        460        470 
EMGVYVVSDG SERPYRVHIR APGFAHLGGF DHLSRGHMLA DAVAVIGTMD LVFGEVDR

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The 49 K subunit of NADH: ubiquinone reductase (complex I) from Neurospora crassa mitochondria: primary structure of the gene and the protein." Preis D., van der Pas J.C., Nehls U., Roehlen D.-A., Sackmann U., Jahnke U., Weiss H. Curr. Genet. 18:59-64(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 43-62. Strain: 74-ORS-6a / FGSC 4200.
[2]	"The genome sequence of the filamentous fungus Neurospora crassa." Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D., Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B., Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M., Qui D. Birren B.W. Nature 422:859-868(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X54508 Genomic DNA. Translation: CAA38368.1. AABX02000006 Genomic DNA. Translation: EAA36429.2.
PIR	S13801.
RefSeq	XP_965665.2. XM_960572.2.
UniGene	Ncr.16312.
3D structure databases
ProteinModelPortal	P22142.
ModBase	Search...
Protein-protein interaction databases
STRING	5141.NCU02534.1.
Protein family/group databases
TCDB	3.D.1.6.2. H+ or Na+-translocating NADH dehydrogenase (NDH) family.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	EFNCRT00000002181; EFNCRP00000002181; EFNCRG00000002178.
GeneID	3881799.
KEGG	ncr:NCU02534.
Phylogenomic databases
eggNOG	COG0649.
HOGENOM	HOG000228264.
KO	K03935.
OrthoDB	EOG4B5SDK.
Family and domain databases
InterPro	IPR001135. NADH_Q_OxRdtase_suD. IPR014029. NADH_UbQ_OxRdtase_49kDa_CS. IPR022885. NDH1_su_D/H. [Graphical view]
Pfam	PF00346. Complex1_49kDa. 1 hit. [Graphical view]
TIGRFAMs	TIGR01962. NuoD. 1 hit.
PROSITE	PS00535. COMPLEX1_49K. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NDUS2_NEUCR

Accession

Primary (citable) accession number: P22142
Secondary accession number(s): Q7RVM8

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1991
Last sequence update:	December 4, 2007
Last modified:	April 3, 2013
This is version 110 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Sites

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

Protocols and materials databases

Genome annotation databases

Phylogenomic databases

Family and domain databases

Entry information

Relevant documents