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P22142

- NDUS2_NEUCR

UniProt

P22142 - NDUS2_NEUCR

Protein

NADH-ubiquinone oxidoreductase 49 kDa subunit, mitochondrial

Gene

nuo-49

Organism
Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 118 (01 Oct 2014)
      Sequence version 2 (04 Dec 2007)
      Previous versions | rss
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    Functioni

    Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

    Catalytic activityi

    NADH + ubiquinone + 5 H+(In) = NAD+ + ubiquinol + 4 H+(Out).
    NADH + acceptor = NAD+ + reduced acceptor.

    Cofactori

    Binds 1 4Fe-4S cluster.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi341 – 3411Iron-sulfur (4Fe-4S)Sequence Analysis
    Metal bindingi347 – 3471Iron-sulfur (4Fe-4S)Sequence Analysis
    Metal bindingi362 – 3621Iron-sulfur (4Fe-4S)Sequence Analysis

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    2. metal ion binding Source: UniProtKB-KW
    3. NAD binding Source: InterPro
    4. NADH dehydrogenase (ubiquinone) activity Source: UniProtKB-EC
    5. quinone binding Source: InterPro

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Electron transport, Respiratory chain, Transport

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Metal-binding, NAD, Ubiquinone

    Protein family/group databases

    TCDBi3.D.1.6.2. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    NADH-ubiquinone oxidoreductase 49 kDa subunit, mitochondrial (EC:1.6.5.3, EC:1.6.99.3)
    Alternative name(s):
    Complex I-49kD
    Short name:
    CI-49kD
    Gene namesi
    Name:nuo-49
    ORF Names:NCU02534
    OrganismiNeurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
    Taxonomic identifieri367110 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesSordariomycetidaeSordarialesSordariaceaeNeurospora
    ProteomesiUP000001805: Chromosome 1, Linkage Group I

    Subcellular locationi

    GO - Cellular componenti

    1. mitochondrial inner membrane Source: UniProtKB-SubCell
    2. respiratory chain Source: UniProtKB-KW

    Keywords - Cellular componenti

    Membrane, Mitochondrion, Mitochondrion inner membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 4242Mitochondrion1 PublicationAdd
    BLAST
    Chaini43 – 478436NADH-ubiquinone oxidoreductase 49 kDa subunit, mitochondrialPRO_0000019984Add
    BLAST

    Interactioni

    Subunit structurei

    Complex I is composed of about 40 different subunits.

    Protein-protein interaction databases

    STRINGi5141.NCU02534.1.

    Structurei

    3D structure databases

    ProteinModelPortaliP22142.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the complex I 49 kDa subunit family.Curated

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG0649.
    HOGENOMiHOG000228264.
    KOiK03935.
    OrthoDBiEOG7B31WZ.

    Family and domain databases

    Gene3Di1.10.645.10. 1 hit.
    HAMAPiMF_01358. NDH1_NuoD.
    InterProiIPR001135. NADH_Q_OxRdtase_suD.
    IPR014029. NADH_UbQ_OxRdtase_49kDa_CS.
    IPR022885. NDH1_su_D/H.
    IPR029014. NiFe_Hase-like.
    [Graphical view]
    PfamiPF00346. Complex1_49kDa. 1 hit.
    [Graphical view]
    SUPFAMiSSF56762. SSF56762. 1 hit.
    TIGRFAMsiTIGR01962. NuoD. 1 hit.
    PROSITEiPS00535. COMPLEX1_49K. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P22142-1 [UniParc]FASTAAdd to Basket

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    MATTLFRLAG RNAKRHCMRQ STTIAHNLNS TRAFSASALR RYAEPSYEGQ    50
    GTRLVPTGDD FAPNNDLYGL EALKADGAPR VPPQDHILAR KVRHYTVNFG 100
    PQHPAAHGVL RLILELKGEE IVRADPHVGL LHRGTEKLCE YRTYLQALPY 150
    FDRLDYVSMM TNEQCFALAV EKLLNVEIPE RAKWIRTMFA EITRILNHLM 200
    SVLSHAMDVG ALTPFLWGFE EREKLMEFYE RVSGARLHAA YVRPGGVHQD 250
    IPLGLLDDIY MWATQFGDRI DETEEMLTDN RIWIDRLRGI GVVSAADALN 300
    LSFTGVMLRG SGVPWDIRKS QPYDAYDQVE FDVPVGINGD CYDRYLCRME 350
    EFRQSLRIIH QCLNKMPAGP VRVEDYKISP PPRSAMKENM EALIHHFLLY 400
    TKGYAVPPGD TYSAIEAPKG EMGVYVVSDG SERPYRVHIR APGFAHLGGF 450
    DHLSRGHMLA DAVAVIGTMD LVFGEVDR 478
    Length:478
    Mass (Da):54,019
    Last modified:December 4, 2007 - v2
    Checksum:i159C35BE3DF9D916
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti417 – 4171A → G in CAA38368. (PubMed:2147127)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X54508 Genomic DNA. Translation: CAA38368.1.
    CM002236 Genomic DNA. Translation: EAA36429.2.
    PIRiS13801.
    RefSeqiXP_965665.2. XM_960572.2.
    UniGeneiNcr.16312.

    Genome annotation databases

    EnsemblFungiiEFNCRT00000002181; EFNCRP00000002181; EFNCRG00000002178.
    GeneIDi3881799.
    KEGGincr:NCU02534.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X54508 Genomic DNA. Translation: CAA38368.1 .
    CM002236 Genomic DNA. Translation: EAA36429.2 .
    PIRi S13801.
    RefSeqi XP_965665.2. XM_960572.2.
    UniGenei Ncr.16312.

    3D structure databases

    ProteinModelPortali P22142.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 5141.NCU02534.1.

    Protein family/group databases

    TCDBi 3.D.1.6.2. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii EFNCRT00000002181 ; EFNCRP00000002181 ; EFNCRG00000002178 .
    GeneIDi 3881799.
    KEGGi ncr:NCU02534.

    Phylogenomic databases

    eggNOGi COG0649.
    HOGENOMi HOG000228264.
    KOi K03935.
    OrthoDBi EOG7B31WZ.

    Family and domain databases

    Gene3Di 1.10.645.10. 1 hit.
    HAMAPi MF_01358. NDH1_NuoD.
    InterProi IPR001135. NADH_Q_OxRdtase_suD.
    IPR014029. NADH_UbQ_OxRdtase_49kDa_CS.
    IPR022885. NDH1_su_D/H.
    IPR029014. NiFe_Hase-like.
    [Graphical view ]
    Pfami PF00346. Complex1_49kDa. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56762. SSF56762. 1 hit.
    TIGRFAMsi TIGR01962. NuoD. 1 hit.
    PROSITEi PS00535. COMPLEX1_49K. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The 49 K subunit of NADH: ubiquinone reductase (complex I) from Neurospora crassa mitochondria: primary structure of the gene and the protein."
      Preis D., van der Pas J.C., Nehls U., Roehlen D.-A., Sackmann U., Jahnke U., Weiss H.
      Curr. Genet. 18:59-64(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 43-62.
      Strain: 74-ORS-6a / FGSC 4200.
    2. "The genome sequence of the filamentous fungus Neurospora crassa."
      Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D., Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B., Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M., Qui D.
      , Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M., Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U., Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D., Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S., Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D., Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S., Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A., DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R., Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R., Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I., Sachs M.S., Lander E.S., Nusbaum C., Birren B.W.
      Nature 422:859-868(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.

    Entry informationi

    Entry nameiNDUS2_NEUCR
    AccessioniPrimary (citable) accession number: P22142
    Secondary accession number(s): Q7RVM8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1991
    Last sequence update: December 4, 2007
    Last modified: October 1, 2014
    This is version 118 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3