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P22142

- NDUS2_NEUCR

UniProt

P22142 - NDUS2_NEUCR

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Protein

NADH-ubiquinone oxidoreductase 49 kDa subunit, mitochondrial

Gene

nuo-49

Organism
Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

Catalytic activityi

NADH + ubiquinone + 5 H+(In) = NAD+ + ubiquinol + 4 H+(Out).
NADH + acceptor = NAD+ + reduced acceptor.

Cofactori

Binds 1 4Fe-4S cluster.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi341 – 3411Iron-sulfur (4Fe-4S)Sequence Analysis
Metal bindingi347 – 3471Iron-sulfur (4Fe-4S)Sequence Analysis
Metal bindingi362 – 3621Iron-sulfur (4Fe-4S)Sequence Analysis

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-KW
  3. NAD binding Source: InterPro
  4. NADH dehydrogenase (ubiquinone) activity Source: UniProtKB-EC
  5. quinone binding Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Respiratory chain, Transport

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, NAD, Ubiquinone

Protein family/group databases

TCDBi3.D.1.6.2. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

Names & Taxonomyi

Protein namesi
Recommended name:
NADH-ubiquinone oxidoreductase 49 kDa subunit, mitochondrial (EC:1.6.5.3, EC:1.6.99.3)
Alternative name(s):
Complex I-49kD
Short name:
CI-49kD
Gene namesi
Name:nuo-49
ORF Names:NCU02534
OrganismiNeurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Taxonomic identifieri367110 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesSordariomycetidaeSordarialesSordariaceaeNeurospora
ProteomesiUP000001805: Chromosome 1, Linkage Group I

Subcellular locationi

GO - Cellular componenti

  1. mitochondrial inner membrane Source: UniProtKB-KW
  2. respiratory chain Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 4242Mitochondrion1 PublicationAdd
BLAST
Chaini43 – 478436NADH-ubiquinone oxidoreductase 49 kDa subunit, mitochondrialPRO_0000019984Add
BLAST

Interactioni

Subunit structurei

Complex I is composed of about 40 different subunits.

Protein-protein interaction databases

STRINGi5141.NCU02534.1.

Structurei

3D structure databases

ProteinModelPortaliP22142.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the complex I 49 kDa subunit family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0649.
HOGENOMiHOG000228264.
InParanoidiP22142.
KOiK03935.
OrthoDBiEOG7B31WZ.

Family and domain databases

Gene3Di1.10.645.10. 1 hit.
HAMAPiMF_01358. NDH1_NuoD.
InterProiIPR001135. NADH_Q_OxRdtase_suD.
IPR014029. NADH_UbQ_OxRdtase_49kDa_CS.
IPR022885. NDH1_su_D/H.
IPR029014. NiFe_Hase-like.
[Graphical view]
PfamiPF00346. Complex1_49kDa. 1 hit.
[Graphical view]
SUPFAMiSSF56762. SSF56762. 1 hit.
TIGRFAMsiTIGR01962. NuoD. 1 hit.
PROSITEiPS00535. COMPLEX1_49K. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P22142-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MATTLFRLAG RNAKRHCMRQ STTIAHNLNS TRAFSASALR RYAEPSYEGQ
60 70 80 90 100
GTRLVPTGDD FAPNNDLYGL EALKADGAPR VPPQDHILAR KVRHYTVNFG
110 120 130 140 150
PQHPAAHGVL RLILELKGEE IVRADPHVGL LHRGTEKLCE YRTYLQALPY
160 170 180 190 200
FDRLDYVSMM TNEQCFALAV EKLLNVEIPE RAKWIRTMFA EITRILNHLM
210 220 230 240 250
SVLSHAMDVG ALTPFLWGFE EREKLMEFYE RVSGARLHAA YVRPGGVHQD
260 270 280 290 300
IPLGLLDDIY MWATQFGDRI DETEEMLTDN RIWIDRLRGI GVVSAADALN
310 320 330 340 350
LSFTGVMLRG SGVPWDIRKS QPYDAYDQVE FDVPVGINGD CYDRYLCRME
360 370 380 390 400
EFRQSLRIIH QCLNKMPAGP VRVEDYKISP PPRSAMKENM EALIHHFLLY
410 420 430 440 450
TKGYAVPPGD TYSAIEAPKG EMGVYVVSDG SERPYRVHIR APGFAHLGGF
460 470
DHLSRGHMLA DAVAVIGTMD LVFGEVDR
Length:478
Mass (Da):54,019
Last modified:December 4, 2007 - v2
Checksum:i159C35BE3DF9D916
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti417 – 4171A → G in CAA38368. (PubMed:2147127)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X54508 Genomic DNA. Translation: CAA38368.1.
CM002236 Genomic DNA. Translation: EAA36429.2.
PIRiS13801.
RefSeqiXP_965665.2. XM_960572.2.
UniGeneiNcr.16312.

Genome annotation databases

EnsemblFungiiEFNCRT00000002181; EFNCRP00000002181; EFNCRG00000002178.
GeneIDi3881799.
KEGGincr:NCU02534.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X54508 Genomic DNA. Translation: CAA38368.1 .
CM002236 Genomic DNA. Translation: EAA36429.2 .
PIRi S13801.
RefSeqi XP_965665.2. XM_960572.2.
UniGenei Ncr.16312.

3D structure databases

ProteinModelPortali P22142.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 5141.NCU02534.1.

Protein family/group databases

TCDBi 3.D.1.6.2. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii EFNCRT00000002181 ; EFNCRP00000002181 ; EFNCRG00000002178 .
GeneIDi 3881799.
KEGGi ncr:NCU02534.

Phylogenomic databases

eggNOGi COG0649.
HOGENOMi HOG000228264.
InParanoidi P22142.
KOi K03935.
OrthoDBi EOG7B31WZ.

Family and domain databases

Gene3Di 1.10.645.10. 1 hit.
HAMAPi MF_01358. NDH1_NuoD.
InterProi IPR001135. NADH_Q_OxRdtase_suD.
IPR014029. NADH_UbQ_OxRdtase_49kDa_CS.
IPR022885. NDH1_su_D/H.
IPR029014. NiFe_Hase-like.
[Graphical view ]
Pfami PF00346. Complex1_49kDa. 1 hit.
[Graphical view ]
SUPFAMi SSF56762. SSF56762. 1 hit.
TIGRFAMsi TIGR01962. NuoD. 1 hit.
PROSITEi PS00535. COMPLEX1_49K. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The 49 K subunit of NADH: ubiquinone reductase (complex I) from Neurospora crassa mitochondria: primary structure of the gene and the protein."
    Preis D., van der Pas J.C., Nehls U., Roehlen D.-A., Sackmann U., Jahnke U., Weiss H.
    Curr. Genet. 18:59-64(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 43-62.
    Strain: 74-ORS-6a / FGSC 4200.
  2. "The genome sequence of the filamentous fungus Neurospora crassa."
    Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D., Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B., Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M., Qui D.
    , Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M., Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U., Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D., Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S., Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D., Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S., Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A., DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R., Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R., Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I., Sachs M.S., Lander E.S., Nusbaum C., Birren B.W.
    Nature 422:859-868(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.

Entry informationi

Entry nameiNDUS2_NEUCR
AccessioniPrimary (citable) accession number: P22142
Secondary accession number(s): Q7RVM8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: December 4, 2007
Last modified: October 29, 2014
This is version 119 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3