Skip Header

Contribute Send feedback
Read comments (?) or add your own

P22140 (EPT1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 112. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Choline/ethanolaminephosphotransferase 1
Short name=ETHPT
Short name=Ethanolaminephosphotransferase 1
EC=2.7.8.1
EC=2.7.8.2
Gene names
Name:EPT1
Ordered Locus Names:YHR123W
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length391 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in protein-dependent process of phospholipid transport to distribute phosphatidyl ethanolamine to the lumenal surface. The multiple transmembrane domains and lumenal hydrophilic domains of the ethanolaminephosphotransferase might participate in the transport process. EPT1 catalyzes both choline- and ethanolamine-phosphotransferase reactions.

Catalytic activity

CDP-ethanolamine + 1,2-diacylglycerol = CMP + a phosphatidylethanolamine.

CDP-choline + 1,2-diacylglycerol = CMP + a phosphatidylcholine.

Cofactor

Phospholipid.

Enzyme regulation

Requires a divalent cation activator, and is inhibited by CMP.

Pathway

Phospholipid metabolism; phosphatidylethanolamine biosynthesis; phosphatidylethanolamine from ethanolamine: step 3/3.

Phospholipid metabolism; phosphatidylcholine biosynthesis; phosphatidylcholine from phosphocholine: step 2/2.

Subcellular location

Microsome membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein.

Sequence similarities

Belongs to the CDP-alcohol phosphatidyltransferase class-I family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 391391Choline/ethanolaminephosphotransferase 1
PRO_0000056809

Regions

Topological domain1 – 4848Lumenal Potential
Transmembrane49 – 6921Helical; Potential
Topological domain70 – 174105Cytoplasmic Potential
Transmembrane175 – 19521Helical; Potential
Transmembrane216 – 23621Helical; Potential
Transmembrane263 – 28321Helical; Potential
Transmembrane289 – 30921Helical; Potential
Transmembrane319 – 33921Helical; Potential
Transmembrane347 – 36721Helical; Potential
Topological domain368 – 39124Cytoplasmic Potential

Experimental info

Sequence conflict961D → V in AAA63572. Ref.1
Sequence conflict981C → S in AAA63572. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P22140 [UniParc].

Last modified February 1, 1995. Version 2.
Checksum: 038942A7FB9EB580

FASTA39144,560
        10         20         30         40         50         60 
MGYFVPDSHI ENLKSYKYQS EDRSLVSKYF LKPFWQRFCH IFPTWMAPNI ITLSGFAFIV 

        70         80         90        100        110        120 
INVLTVFYYD PNLNTDTPRW TYFSYALGVF LYQTFDGCDG VHARRINQSG PLGELFDHSI 

       130        140        150        160        170        180 
DAINSTLSIF IFASETGMGF SYNLMLSQFA MLTNFYLSTW EEYHTHTLYL SEFSGPVEGI 

       190        200        210        220        230        240 
LIVCVSLILT GIYGKQVIWH TYLFTITVGD KVIDVDTLDI VFSLAVFGLV MNALSAKRNV 

       250        260        270        280        290        300 
DKYYRNSTSS ANNITQIEQD SAIKGLLPFF AYYASIALLV WMQPSFITLS FILSVGFTGA 

       310        320        330        340        350        360 
FTVGRIIVCH LTKQSFPMFN APMLIPLCQI VLYKICLSLW GIESNKIVFA LSWLGFGLSL 

       370        380        390 
GVHIMFMNDI IHEFTEYLDV YALSIKRSKL T

« Hide

References

« Hide 'large scale' references
[1]"sn-1,2-diacylglycerol choline- and ethanolaminephosphotransferases in Saccharomyces cerevisiae. Nucleotide sequence of the EPT1 gene and comparison of the CPT1 and EPT1 gene products."
Hjelmstad R.H., Bell R.M.
J. Biol. Chem. 266:5094-5103(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204660 / DBY746.
[2]"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII."
Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z., Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T., Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P. expand/collapse author list , Louis E.J., Macri C., Mardis E., Menezes S., Mouser L., Nhan M., Rifkin L., Riles L., St Peter H., Trevaskis E., Vaughan K., Vignati D., Wilcox L., Wohldman P., Waterston R., Wilson R., Vaudin M.
Science 265:2077-2082(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204511 / S288c / AB972.
[3]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"sn-1,2-diacylglycerol choline- and ethanolaminephosphotransferases in Saccharomyces cerevisiae. Mixed micellar analysis of the CPT1 and EPT1 gene products."
Hjelmstad R.H., Bell R.M.
J. Biol. Chem. 266:4357-4365(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		M59311 Genomic DNA. Translation: AAA63572.1.
U10398 Genomic DNA. Translation: AAB68409.1.
BK006934 Genomic DNA. Translation: DAA06817.1.
PIRS48967.
RefSeqNP_011991.1. NM_001179253.1.

3D structure databases

ProteinModelPortalP22140.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP-5619N.
IntActP22140. 10 interactions.
MINTMINT-475142.
STRING4932.YHR123W.

Proteomic databases

PaxDbP22140.
PeptideAtlasP22140.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYHR123W; YHR123W; YHR123W.
GeneID856523.
KEGGsce:YHR123W.

Organism-specific databases

CYGDYHR123w.
SGDS000001165. EPT1.

Phylogenomic databases

eggNOGCOG5050.
GeneTreeENSGT00530000063048.
HOGENOMHOG000157893.
KOK00993.
OMATIDKSIM.
OrthoDBEOG4K9FN5.

Enzyme and pathway databases

UniPathwayUPA00558; UER00743.
UPA00753; UER00740.

Gene expression databases

GenevestigatorP22140.
GermOnlineYHR123W. Saccharomyces cerevisiae.

Family and domain databases

InterProIPR000462. CDP-OH_P_trans.
IPR014472. CHOPT.
[Graphical view]
PfamPF01066. CDP-OH_P_transf. 1 hit.
[Graphical view]
PIRSFPIRSF015665. CHOPT. 1 hit.
PROSITEPS00379. CDP_ALCOHOL_P_TRANSF. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio982287.

Entry information

Entry nameEPT1_YEAST
AccessionPrimary (citable) accession number: P22140
Secondary accession number(s): D3DL73
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: February 1, 1995
Last modified: May 1, 2013
This is version 112 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome VIII

Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents