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Protein

Clathrin heavy chain

Gene

CHC1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. In yeast, it is involved in the retention of proteins in an intracellular membrane compartment, presumably the trans-Golgi.1 Publication

GO - Molecular functioni

  • structural molecule activity Source: SGD

GO - Biological processi

  • endocytosis Source: SGD
  • intracellular protein transport Source: InterPro
  • vesicle-mediated transport Source: SGD
Complete GO annotation...

Enzyme and pathway databases

BioCyciYEAST:G3O-30683-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Clathrin heavy chain
Gene namesi
Name:CHC1
Ordered Locus Names:YGL206C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGL206C.
SGDiS000003174. CHC1.

Subcellular locationi

GO - Cellular componenti

  • clathrin coat of coated pit Source: InterPro
  • clathrin coat of trans-Golgi network vesicle Source: InterPro
  • clathrin vesicle coat Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Coated pit, Cytoplasmic vesicle, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 16531653Clathrin heavy chainPRO_0000205785Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki1107 – 1107Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Proteomic databases

MaxQBiP22137.
PRIDEiP22137.

PTM databases

iPTMnetiP22137.

Interactioni

Subunit structurei

Clathrin triskelions, composed of 3 heavy chains and 3 light chains, are the basic subunits of the clathrin coat. Interacts with the auxilin-like clathrin uncoating factor SWA2. Interacts with INP53.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
APL2P360003EBI-4766,EBI-2206
SLA1P327904EBI-4766,EBI-17313
YAP1801P388562EBI-4766,EBI-24811

Protein-protein interaction databases

BioGridi33050. 102 interactions.
DIPiDIP-2279N.
IntActiP22137. 53 interactions.
MINTiMINT-372536.

Structurei

3D structure databases

ProteinModelPortaliP22137.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati543 – 689147CHCR 1Add
BLAST
Repeati692 – 834143CHCR 2Add
BLAST
Repeati839 – 978140CHCR 3Add
BLAST
Repeati985 – 1130146CHCR 4Add
BLAST
Repeati1134 – 1275142CHCR 5Add
BLAST
Repeati1280 – 1426147CHCR 6Add
BLAST
Repeati1429 – 1572144CHCR 7Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni? – 1653Proximal segment
Regioni1 – 483483Globular terminal domainAdd
BLAST
Regioni23 – 6644WD40-like repeat 1Add
BLAST
Regioni67 – 10741WD40-like repeat 2Add
BLAST
Regioni108 – 15245WD40-like repeat 3Add
BLAST
Regioni153 – 19846WD40-like repeat 4Add
BLAST
Regioni199 – 26365WD40-like repeat 5Add
BLAST
Regioni264 – 30744WD40-like repeat 6Add
BLAST
Regioni308 – 33629WD40-like repeat 7Add
BLAST
Regioni453 – 46917Binding site for the uncoating ATPase, involved in lattice disassemblySequence analysisAdd
BLAST
Regioni484 – 52744Flexible linkerAdd
BLAST
Regioni528 – 16531126Heavy chain armAdd
BLAST
Regioni528 – ?Distal segment
Regioni1219 – 1528310Involved in binding clathrin light chainBy similarityAdd
BLAST

Domaini

The C-terminal third of the heavy chains forms the hub of the triskelion. This region contains the trimerization domain and the light-chain binding domain involved in the assembly of the clathrin lattice.
The N-terminal seven-bladed beta-propeller is formed by WD40-like repeats, and projects inward from the polyhedral outer clathrin coat. It consitutes a major protein-protein interaction node (By similarity).By similarity

Sequence similaritiesi

Belongs to the clathrin heavy chain family.Curated
Contains 7 CHCR (clathrin heavy-chain) repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

GeneTreeiENSGT00400000022107.
HOGENOMiHOG000188877.
InParanoidiP22137.
KOiK04646.
OMAiMNEDIVY.
OrthoDBiEOG7V4B6Q.

Family and domain databases

Gene3Di1.25.40.10. 3 hits.
2.130.10.110. 1 hit.
InterProiIPR016024. ARM-type_fold.
IPR000547. Clathrin_H-chain/VPS_repeat.
IPR016025. Clathrin_H-chain_link/propller.
IPR015348. Clathrin_H-chain_linker_core.
IPR001473. Clathrin_H-chain_propeller_N.
IPR022365. Clathrin_H-chain_propeller_rpt.
IPR016341. Clathrin_heavy_chain.
IPR011990. TPR-like_helical_dom.
[Graphical view]
PfamiPF00637. Clathrin. 7 hits.
PF09268. Clathrin-link. 1 hit.
PF01394. Clathrin_propel. 1 hit.
[Graphical view]
PIRSFiPIRSF002290. Clathrin_H_chain. 1 hit.
SMARTiSM00299. CLH. 7 hits.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 6 hits.
SSF50989. SSF50989. 1 hit.
PROSITEiPS50236. CHCR. 7 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P22137-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDLPIEFTE LVDLMSLGIS PQFLDFRSTT FESDHFVTVR ETKDGTNSVA
60 70 80 90 100
IVDLAKGNEV TRKNMGGDSA IMHPSQMVIS VRANGTIVQI FNLETKSKLK
110 120 130 140 150
SFTLDEPVIF WRWLSETTLG FVTARSILTS NVFDGNVNAK PQLLTLRHAN
160 170 180 190 200
LNNTQIINFV ANKNLDWFAV VGILQENGRI AGRIQLFSKQ RNISQAIDGH
210 220 230 240 250
VAIFTNILLE GNGSTPVQVF VTGNRNATTG AGELRIIEID HDASLPSQYQ
260 270 280 290 300
KETTDIFFPP DATNDFPIAV QVSEKYGIIY LLTKYGFIHL YELETGTNLF
310 320 330 340 350
VNRITAESVF TAAPYNHENG IACINKKGQV LAVEISTSQI VPYILNKLSN
360 370 380 390 400
VALALIVATR GGLPGADDLF QKQFESLLLQ NDYQNAAKVA ASSTSLRNQN
410 420 430 440 450
TINRLKNIQA PPGAISPILL YFSTLLDKGK LNKEETIELA RPVLQQDRKQ
460 470 480 490 500
LFEKWLKEDK LECSEELGDI VKPFDTTLAL ACYLRAGAHA KVISCLAELQ
510 520 530 540 550
QFEKIIPYCQ KVGYQPNFLV LISSLIRSSP DRASEFAVSL LQNPETASQI
560 570 580 590 600
DIEKIADLFF SQNHIQQGTS LLLDALKGDT PDQGHLQTRV LEVNLLHAPQ
610 620 630 640 650
VADAILGNNI FSHYDKPTIA SLSEKAGLYQ RALENYTDIK DIKRCVVHTN
660 670 680 690 700
ALPIDWLVGY FGKLNVEQSL ACLKALMDNN IQANIQTVVQ VATKFSDLIG
710 720 730 740 750
PSTLIKLFED YNATEGLYYY LASLVNLTED KDVVYKYIEA AAKMKQYREI
760 770 780 790 800
ERIVKDNNVY DPERVKNFLK DANLEDQLPL VIVCDRFDFV HEMILYLYKS
810 820 830 840 850
QNLKFIETYV QQVNPSKTAQ VVGALLDMDC DEAFIQSLLQ SVLGQVPINE
860 870 880 890 900
LTTEVEKRNR LKILLPFLEQ SLSQGIQDQA VYNALAKIYI DSNNSPEKFL
910 920 930 940 950
KENDQYDTLD VGHYCEKRDP YLAYIAYEKG QNDDDLIRIT NENSMYKYQA
960 970 980 990 1000
RYLLERSDLD LWNKVLNQEN IHRRQLIDSV ISVGIPELTD PEPVSLTVQA
1010 1020 1030 1040 1050
FMTNGLKLEL IELLEKIILE PSPFNENVAL QGLLLLSAIK YEPTKVSSYI
1060 1070 1080 1090 1100
EKLDNYDADE IAPLCIEHDL KEEAFEIYDK HEMYGKALKV LIEDIMSLDR
1110 1120 1130 1140 1150
AASYADKINT PELWSQIGTA QLDGLRIPDA IESYIKAEDP SNYENVIDIA
1160 1170 1180 1190 1200
EQAGKYEELI PFLLMARKTL KEPKIDGALI LAYAELNKIH EIENLLAGSN
1210 1220 1230 1240 1250
VANLDHVGDK LFENKEYKAA RLCYSAVSNY SKLASTLVYL GDYQAAVDTA
1260 1270 1280 1290 1300
RKASNIKVWK LVNDACIEKK EFKLAQICGL NLIVHAEELD ELVERYESNG
1310 1320 1330 1340 1350
YFEELISLFE AGLGLERAHM GMFTELAILY SKYEPDKTFE HLKLFWSRIN
1360 1370 1380 1390 1400
IPKVIRAVEQ AHLWSELVFL YAHYDEWDNA ALTLIEKSTK DLDHAYFKEV
1410 1420 1430 1440 1450
VVKVSNLEIY YKAINFYVKF HPSLLVDLLT SLTPRLDIPR TVKIFSKSDN
1460 1470 1480 1490 1500
LPLIKPFLIN VLPKNNSVVN QAYHDLMIEE EDYKALQDAV DSYDKFDQLG
1510 1520 1530 1540 1550
LASRLESHKL IFFKKIGALL YRRNKKWAKS LSILKEEKLW KDAIETAAIS
1560 1570 1580 1590 1600
QDPKVVEALL TYFVETGNRE GFVALLYAAY NLVRIEFVLE ISWMNSLEDY
1610 1620 1630 1640 1650
IKPFEISIKK EQNDSIKKIT EELAKKSGSN EEHKDGQPLM LMNSAMNVQP

TGF
Length:1,653
Mass (Da):187,234
Last modified:August 1, 1991 - v1
Checksum:i622F3083DF2FE315
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52900 Genomic DNA. Translation: CAA37082.1.
Z72728 Genomic DNA. Translation: CAA96919.1.
BK006941 Genomic DNA. Translation: DAA07910.1.
PIRiA36349.
RefSeqiNP_011309.1. NM_001181071.1.

Genome annotation databases

EnsemblFungiiYGL206C; YGL206C; YGL206C.
GeneIDi852666.
KEGGisce:YGL206C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52900 Genomic DNA. Translation: CAA37082.1.
Z72728 Genomic DNA. Translation: CAA96919.1.
BK006941 Genomic DNA. Translation: DAA07910.1.
PIRiA36349.
RefSeqiNP_011309.1. NM_001181071.1.

3D structure databases

ProteinModelPortaliP22137.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33050. 102 interactions.
DIPiDIP-2279N.
IntActiP22137. 53 interactions.
MINTiMINT-372536.

PTM databases

iPTMnetiP22137.

Proteomic databases

MaxQBiP22137.
PRIDEiP22137.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYGL206C; YGL206C; YGL206C.
GeneIDi852666.
KEGGisce:YGL206C.

Organism-specific databases

EuPathDBiFungiDB:YGL206C.
SGDiS000003174. CHC1.

Phylogenomic databases

GeneTreeiENSGT00400000022107.
HOGENOMiHOG000188877.
InParanoidiP22137.
KOiK04646.
OMAiMNEDIVY.
OrthoDBiEOG7V4B6Q.

Enzyme and pathway databases

BioCyciYEAST:G3O-30683-MONOMER.

Miscellaneous databases

PROiP22137.

Family and domain databases

Gene3Di1.25.40.10. 3 hits.
2.130.10.110. 1 hit.
InterProiIPR016024. ARM-type_fold.
IPR000547. Clathrin_H-chain/VPS_repeat.
IPR016025. Clathrin_H-chain_link/propller.
IPR015348. Clathrin_H-chain_linker_core.
IPR001473. Clathrin_H-chain_propeller_N.
IPR022365. Clathrin_H-chain_propeller_rpt.
IPR016341. Clathrin_heavy_chain.
IPR011990. TPR-like_helical_dom.
[Graphical view]
PfamiPF00637. Clathrin. 7 hits.
PF09268. Clathrin-link. 1 hit.
PF01394. Clathrin_propel. 1 hit.
[Graphical view]
PIRSFiPIRSF002290. Clathrin_H_chain. 1 hit.
SMARTiSM00299. CLH. 7 hits.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 6 hits.
SSF50989. SSF50989. 1 hit.
PROSITEiPS50236. CHCR. 7 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence of the clathrin heavy chain from Saccharomyces cerevisiae and requirement of the COOH terminus for clathrin function."
    Lemmon S.K., Pellicena-Palle A., Conley K., Freund C.L.
    J. Cell Biol. 112:65-80(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. "Analysis of 21.7 kb DNA sequence from the left arm of chromosome VII reveals 11 open reading frames: two correspond to new genes."
    Feuermann M., Simeonava L., Souciet J.-L., Potier S.
    Yeast 13:475-477(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
    Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
    , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
    Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. "The synaptojanin-like protein Inp53/Sjl3 functions with clathrin in a yeast TGN-to-endosome pathway distinct from the GGA protein-dependent pathway."
    Ha S.-A., Torabinejad J., DeWald D.B., Wenk M.R., Lucast L., De Camilli P., Newitt R.A., Aebersold R., Nothwehr S.F.
    Mol. Biol. Cell 14:1319-1333(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH INP53.
  6. "Dissection of Swa2p/auxilin domain requirements for cochaperoning Hsp70 clathrin-uncoating activity in vivo."
    Xiao J., Kim L.S., Graham T.R.
    Mol. Biol. Cell 17:3281-3290(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SWA2.
  7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  8. "Sites of ubiquitin attachment in Saccharomyces cerevisiae."
    Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
    Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-1107, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiCLH_YEAST
AccessioniPrimary (citable) accession number: P22137
Secondary accession number(s): D6VTU9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: August 1, 1991
Last modified: July 6, 2016
This is version 148 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 28700 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.