P22137 (CLH_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Clathrin heavy chain

Gene names

Name:	CHC1
Ordered Locus Names:	YGL206C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	1653 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. In yeast, it is involved in the retention of proteins in an intracellular membrane compartment, presumably the trans-Golgi. Ref.5
Subunit structure	Clathrin triskelions, composed of 3 heavy chains and 3 light chains, are the basic subunits of the clathrin coat. Interacts with the auxilin-like clathrin uncoating factor SWA2. Interacts with INP53. Ref.5 Ref.6
Subcellular location	Cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side. Membrane › coated pit; Peripheral membrane protein; Cytoplasmic side. Note: Cytoplasmic face of coated pits and vesicles.
Domain	The C-terminal third of the heavy chains forms the hub of the triskelion. This region contains the trimerization domain and the light-chain binding domain involved in the assembly of the clathrin lattice.
Miscellaneous	Present with 28700 molecules/cell in log phase SD medium. Ref.7
Sequence similarities	Belongs to the clathrin heavy chain family.

Ontologies

Keywords
Cellular component	Coated pit Cytoplasmic vesicle Membrane
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	endocytosis Inferred from direct assay. Source: SGD intracellular protein transport Inferred from electronic annotation. Source: InterPro
Cellular component	clathrin coat of coated pit Inferred from electronic annotation. Source: InterPro clathrin coat of trans-Golgi network vesicle Inferred from electronic annotation. Source: InterPro
Molecular function	protein binding Inferred from physical interaction. Source: IntAct structural molecule activity Traceable author statement. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
APL2	P36000	3	EBI-4766,EBI-2206
YAP1801	P38856	2	EBI-4766,EBI-24811

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1653	1653	Clathrin heavy chain		PRO_0000205785
Regions
	Region	1 – 483	483	Globular terminal domain
	Region	453 – 469	17	Binding site for the uncoating ATPase, involved in lattice disassembly Potential
	Region	484 – 527	44	Flexible linker
	Region	528 – 1653	1126	Heavy chain arm
	Region	528 – ?		Distal segment
	Region	1219 – 1528	310	Involved in binding clathrin light chain By similarity
	Region	? – 1653		Proximal segment
Amino acid modifications
	Modified residue	892	1	Phosphoserine Ref.8

Sequences

	Sequence		Length	Mass (Da)
	P22137 [UniParc]. Last modified August 1, 1991. Version 1. Checksum: 622F3083DF2FE315	FASTA	1,653	187,234
	10 20 30 40 50 60 MSDLPIEFTE LVDLMSLGIS PQFLDFRSTT FESDHFVTVR ETKDGTNSVA IVDLAKGNEV 70 80 90 100 110 120 TRKNMGGDSA IMHPSQMVIS VRANGTIVQI FNLETKSKLK SFTLDEPVIF WRWLSETTLG 130 140 150 160 170 180 FVTARSILTS NVFDGNVNAK PQLLTLRHAN LNNTQIINFV ANKNLDWFAV VGILQENGRI 190 200 210 220 230 240 AGRIQLFSKQ RNISQAIDGH VAIFTNILLE GNGSTPVQVF VTGNRNATTG AGELRIIEID 250 260 270 280 290 300 HDASLPSQYQ KETTDIFFPP DATNDFPIAV QVSEKYGIIY LLTKYGFIHL YELETGTNLF 310 320 330 340 350 360 VNRITAESVF TAAPYNHENG IACINKKGQV LAVEISTSQI VPYILNKLSN VALALIVATR 370 380 390 400 410 420 GGLPGADDLF QKQFESLLLQ NDYQNAAKVA ASSTSLRNQN TINRLKNIQA PPGAISPILL 430 440 450 460 470 480 YFSTLLDKGK LNKEETIELA RPVLQQDRKQ LFEKWLKEDK LECSEELGDI VKPFDTTLAL 490 500 510 520 530 540 ACYLRAGAHA KVISCLAELQ QFEKIIPYCQ KVGYQPNFLV LISSLIRSSP DRASEFAVSL 550 560 570 580 590 600 LQNPETASQI DIEKIADLFF SQNHIQQGTS LLLDALKGDT PDQGHLQTRV LEVNLLHAPQ 610 620 630 640 650 660 VADAILGNNI FSHYDKPTIA SLSEKAGLYQ RALENYTDIK DIKRCVVHTN ALPIDWLVGY 670 680 690 700 710 720 FGKLNVEQSL ACLKALMDNN IQANIQTVVQ VATKFSDLIG PSTLIKLFED YNATEGLYYY 730 740 750 760 770 780 LASLVNLTED KDVVYKYIEA AAKMKQYREI ERIVKDNNVY DPERVKNFLK DANLEDQLPL 790 800 810 820 830 840 VIVCDRFDFV HEMILYLYKS QNLKFIETYV QQVNPSKTAQ VVGALLDMDC DEAFIQSLLQ 850 860 870 880 890 900 SVLGQVPINE LTTEVEKRNR LKILLPFLEQ SLSQGIQDQA VYNALAKIYI DSNNSPEKFL 910 920 930 940 950 960 KENDQYDTLD VGHYCEKRDP YLAYIAYEKG QNDDDLIRIT NENSMYKYQA RYLLERSDLD 970 980 990 1000 1010 1020 LWNKVLNQEN IHRRQLIDSV ISVGIPELTD PEPVSLTVQA FMTNGLKLEL IELLEKIILE 1030 1040 1050 1060 1070 1080 PSPFNENVAL QGLLLLSAIK YEPTKVSSYI EKLDNYDADE IAPLCIEHDL KEEAFEIYDK 1090 1100 1110 1120 1130 1140 HEMYGKALKV LIEDIMSLDR AASYADKINT PELWSQIGTA QLDGLRIPDA IESYIKAEDP 1150 1160 1170 1180 1190 1200 SNYENVIDIA EQAGKYEELI PFLLMARKTL KEPKIDGALI LAYAELNKIH EIENLLAGSN 1210 1220 1230 1240 1250 1260 VANLDHVGDK LFENKEYKAA RLCYSAVSNY SKLASTLVYL GDYQAAVDTA RKASNIKVWK 1270 1280 1290 1300 1310 1320 LVNDACIEKK EFKLAQICGL NLIVHAEELD ELVERYESNG YFEELISLFE AGLGLERAHM 1330 1340 1350 1360 1370 1380 GMFTELAILY SKYEPDKTFE HLKLFWSRIN IPKVIRAVEQ AHLWSELVFL YAHYDEWDNA 1390 1400 1410 1420 1430 1440 ALTLIEKSTK DLDHAYFKEV VVKVSNLEIY YKAINFYVKF HPSLLVDLLT SLTPRLDIPR 1450 1460 1470 1480 1490 1500 TVKIFSKSDN LPLIKPFLIN VLPKNNSVVN QAYHDLMIEE EDYKALQDAV DSYDKFDQLG 1510 1520 1530 1540 1550 1560 LASRLESHKL IFFKKIGALL YRRNKKWAKS LSILKEEKLW KDAIETAAIS QDPKVVEALL 1570 1580 1590 1600 1610 1620 TYFVETGNRE GFVALLYAAY NLVRIEFVLE ISWMNSLEDY IKPFEISIKK EQNDSIKKIT 1630 1640 1650 EELAKKSGSN EEHKDGQPLM LMNSAMNVQP TGF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence of the clathrin heavy chain from Saccharomyces cerevisiae and requirement of the COOH terminus for clathrin function." Lemmon S.K., Pellicena-Palle A., Conley K., Freund C.L. J. Cell Biol. 112:65-80(1991) [PubMed: 1898742] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"Analysis of 21.7 kb DNA sequence from the left arm of chromosome VII reveals 11 open reading frames: two correspond to new genes." Feuermann M., Simeonava L., Souciet J.-L., Potier S. Yeast 13:475-477(1997) [PubMed: 9153757] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K. Nature 387:81-84(1997) [PubMed: 9169869] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"The synaptojanin-like protein Inp53/Sjl3 functions with clathrin in a yeast TGN-to-endosome pathway distinct from the GGA protein-dependent pathway." Ha S.-A., Torabinejad J., DeWald D.B., Wenk M.R., Lucast L., De Camilli P., Newitt R.A., Aebersold R., Nothwehr S.F. Mol. Biol. Cell 14:1319-1333(2003) [PubMed: 12686590] [Abstract] Cited for: FUNCTION, INTERACTION WITH INP53.
[6]	"Dissection of Swa2p/auxilin domain requirements for cochaperoning Hsp70 clathrin-uncoating activity in vivo." Xiao J., Kim L.S., Graham T.R. Mol. Biol. Cell 17:3281-3290(2006) [PubMed: 16687570] [Abstract] Cited for: INTERACTION WITH SWA2.
[7]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-892, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X52900 Genomic DNA. Translation: CAA37082.1. Z72728 Genomic DNA. Translation: CAA96919.1. BK006941 Genomic DNA. Translation: DAA07910.1.
PIR	A36349.
RefSeq	NP_011309.1. NM_001181071.1.
3D structure databases
ProteinModelPortal	P22137.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2279N.
IntAct	P22137. 47 interactions.
MINT	MINT-372536.
STRING	P22137.
Proteomic databases
PeptideAtlas	P22137.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YGL206C; YGL206C; YGL206C.
GeneID	852666.
KEGG	sce:YGL206C.
NMPDR	fig|4932.3.peg.2412.
Organism-specific databases
CYGD	YGL206c.
SGD	S000003174. CHC1.
Phylogenomic databases
eggNOG	fuNOG06807.
GeneTree	EFGT00050000000413.
HOGENOM	HBG317411.
OMA	GFIHLYE.
OrthoDB	EOG4DFSWK.
Gene expression databases
ArrayExpress	P22137.
Genevestigator	P22137.
GermOnline	YGL206C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR016024. ARM-type_fold. IPR000547. Clathrin_H-chain/VPS_repeat. IPR016025. Clathrin_H-chain_link/propller. IPR015348. Clathrin_H-chain_linker_core. IPR001473. Clathrin_H-chain_propeller_N. IPR022365. Clathrin_H-chain_propeller_rpt. IPR016341. Clathrin_heavy_chain. IPR011990. TPR-like_helical. [Graphical view]
Gene3D	G3DSA:2.130.10.110. Clathrin_H-chain_link/propller. 1 hit. G3DSA:1.25.40.10. TPR-like_helical. 3 hits.
KO	K04646.
Pfam	PF00637. Clathrin. 7 hits. PF09268. Clathrin-link. 1 hit. PF01394. Clathrin_propel. 1 hit. [Graphical view]
PIRSF	PIRSF002290. Clathrin_H_chain. 1 hit.
SMART	SM00299. CLH. 7 hits. [Graphical view]
SUPFAM	SSF48371. ARM-type_fold. 6 hits. SSF50989. Clathrin_H-chain_propeller_N. 1 hit.
ProtoNet	Search...
Other
NextBio	971960.

Entry information

Entry name

CLH_YEAST

Accession

Primary (citable) accession number: P22137
Secondary accession number(s): D6VTU9

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1991
Last sequence update:	August 1, 1991
Last modified:	December 14, 2011
This is version 108 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program