Reviewed, UniProtKB/Swiss-Prot P22135 (ATP12_YEAST)

Names and origin

Protein names

Recommended name:
    Protein ATP12, mitochondrial

Gene names

Name:	ATP12
Ordered Locus Names:	YJL180C
ORF Names:	J0486

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	325 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Essential for the assembly of the mitochondrial F1-F0 complex. Ref.1
Subunit structure	Exists either as a homo- or heterooligomer. Ref.1
Subcellular location	Mitochondrion. Note: Mitochondrial, either as a constituent of the matrix, or in tenous association with the internal side of the inner membrane. Ref.1
Miscellaneous	Present with 6370 molecules/cell in log phase SD medium. Ref.3
Sequence similarities	Belongs to the ATP12 family.

Ontologies

Keywords
Cellular component	Mitochondrion
Domain	Transit peptide
Molecular function	Chaperone
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	mitochondrial proton-transporting ATP synthase complex assembly Inferred from mutant phenotype. Source: SGD
Cellular component	mitochondrion Ref.1 Inferred from direct assay. Source: SGD
Molecular function	protein domain specific binding Inferred from physical interaction. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
BZZ1	P38822	1	EBI-3312,EBI-3889
FMC1	P40491	1	EBI-3312,EBI-25141

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?32	32	Mitochondrion Potential
	Chain	?33 – 325	293	Protein ATP12, mitochondrial		PRO_0000002421
Experimental info
	Sequence conflict	48	1	S → N in AAA34442. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P22135-1 [UniParc]. Last modified November 1, 1995. Version 2. Checksum: 0571C6C493E12CB1	FASTA	325	36,554
	10 20 30 40 50 60 MLPSLRKGCF IVNSIRLKLP RFYSLNAQPL GTDNTIENNT PTETNRLSKT SQKFWEKVSL 70 80 90 100 110 120 NRDVEKGKIA LQLDGRTIKT PLGNGIIVDN AKSLLAYLLK LEWSSLSSLS IKTHSLPLTS 130 140 150 160 170 180 LVARCIDLQM TNEPGCDPQL VAKIGGNSDV IKNQLLRYLD TDTLLVFSPM NEFEGRLRNA 190 200 210 220 230 240 QNELYIPIIK GMEEFLRNFS SESNIRLQIL DADIHGLRGN QQSDIVKNAA KKYMSSLSPW 250 260 270 280 290 300 DLAILEKTVL TTKSFICGVL LLENKKDTAN LIPALKTDMD NIVRAATLET IFQVEKWGEV 310 320 EDTHDVDKRD IRRKIHTAAI AAFKQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of ATP12, a yeast nuclear gene required for the assembly of the mitochondrial F1-ATPase." Bowman S., Ackerman S.H., Griffiths D.E., Tzagoloff A. J. Biol. Chem. 266:7517-7523(1991) [PubMed: 1826907] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBUNIT, SUBCELLULAR LOCATION.
[2]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X." Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L. EMBO J. 15:2031-2049(1996) [PubMed: 8641269] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M61773 Genomic DNA. Translation: AAA34442.1. Z49455 Genomic DNA. Translation: CAA89475.1.
PIR	S56963.
RefSeq	NP_012355.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:3027N.
IntAct	P22135. 4 interactions.
STRING	P22135.
Genome annotation databases
Ensembl	YJL180C; YJL180C; YJL180C; Saccharomyces cerevisiae. [Genome view]
GeneID	853259.
KEGG	sce:YJL180C.
NMPDR	fig|4932.3.peg.3319.
Organism-specific databases
CYGD	YJL180c.
SGD	S000003716. ATP12.
Phylogenomic databases
HOGENOM	P22135.
OMA	RYLDTDT
OrthoDB	EOG9FFFJ6
Gene expression databases
ArrayExpress	P22135.
Genevestigator	P22135.
GermOnline	YJL180C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR011419. ATP12_ATPase-F1F0-assembly. [Graphical view]
PANTHER	PTHR21013. ATP12. 1 hit.
Pfam	PF07542. ATP12. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	973514.

Entry information

Entry name

ATP12_YEAST

Accession

Primary (citable) accession number: P22135

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1991
Last sequence update:	November 1, 1995
Last modified:	November 24, 2009
This is version 71 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)