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Protein

DNA-3-methyladenine glycosylase

Gene

MAG1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine or 7-methyladenine from the damaged DNA polymer formed by alkylation lesions.

Catalytic activityi

Hydrolysis of alkylated DNA, releasing 3-methyladenine, 3-methylguanine, 7-methylguanine and 7-methyladenine.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei189Determinant for substrate specificity and/or activityBy similarity1
Active sitei209Proton acceptorBy similarity1

GO - Molecular functioni

GO - Biological processi

  • base-excision repair, AP site formation Source: SGD
  • DNA dealkylation involved in DNA repair Source: SGD

Keywordsi

Molecular functionHydrolase
Biological processDNA damage, DNA repair

Enzyme and pathway databases

BioCyciYEAST:G3O-30303-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-3-methyladenine glycosylase (EC:3.2.2.21)
Alternative name(s):
3-methyladenine DNA glycosidase
3MEA DNA glycosylase
Gene namesi
Name:MAG1
Synonyms:MAG
Ordered Locus Names:YER142C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome V

Organism-specific databases

EuPathDBiFungiDB:YER142C
SGDiS000000944 MAG1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001948831 – 296DNA-3-methyladenine glycosylaseAdd BLAST296

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei110PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP22134
PaxDbiP22134
PRIDEiP22134

PTM databases

iPTMnetiP22134

Expressioni

Inductioni

By DNA damage.

Interactioni

Protein-protein interaction databases

BioGridi36891, 114 interactors
DIPiDIP-6599N
IntActiP22134, 25 interactors
STRINGi4932.YER142C

Structurei

3D structure databases

ProteinModelPortaliP22134
SMRiP22134
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000113466
InParanoidiP22134
KOiK01247
OMAiWSAKMFA
OrthoDBiEOG092C51YL

Family and domain databases

CDDicd00056 ENDO3c, 1 hit
InterProiView protein in InterPro
IPR000035 Alkylbase_DNA_glycsylse_CS
IPR011257 DNA_glycosylase
IPR003265 HhH-GPD_domain
PfamiView protein in Pfam
PF00730 HhH-GPD, 1 hit
SMARTiView protein in SMART
SM00478 ENDO3c, 1 hit
SUPFAMiSSF48150 SSF48150, 2 hits
PROSITEiView protein in PROSITE
PS00516 ALKYLBASE_DNA_GLYCOS, 1 hit

Sequencei

Sequence statusi: Complete.

P22134-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLKREYDEL IKADAVKEIA KELGSRPLEV ALPEKYIARH EEKFNMACEH
60 70 80 90 100
ILEKDPSLFP ILKNNEFTLY LKETQVPNTL EDYFIRLAST ILSQQISGQA
110 120 130 140 150
AESIKARVVS LYGGAFPDYK ILFEDFKDPA KCAEIAKCGL SKRKMIYLES
160 170 180 190 200
LAVYFTEKYK DIEKLFGQKD NDEEVIESLV TNVKGIGPWS AKMFLISGLK
210 220 230 240 250
RMDVFAPEDL GIARGFSKYL SDKPELEKEL MRERKVVKKS KIKHKKYNWK
260 270 280 290
IYDDDIMEKC SETFSPYRSV FMFILWRLAS TNTDAMMKAE ENFVKS
Length:296
Mass (Da):34,333
Last modified:August 1, 1991 - v1
Checksum:i14CDD51CA6645507
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X56662 Genomic DNA Translation: CAA39989.1
X57781 Genomic DNA Translation: CAA40927.1
U18917 Genomic DNA Translation: AAB64669.1
AY692938 Genomic DNA Translation: AAT92957.1
BK006939 Genomic DNA Translation: DAA07803.1
PIRiS12498
RefSeqiNP_011069.1, NM_001179032.1

Genome annotation databases

EnsemblFungiiYER142C; YER142C; YER142C
GeneIDi856885
KEGGisce:YER142C

Similar proteinsi

Entry informationi

Entry nameiMAG_YEAST
AccessioniPrimary (citable) accession number: P22134
Secondary accession number(s): D3DM49
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: August 1, 1991
Last modified: March 28, 2018
This is version 157 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome
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Main funding by: National Institutes of Health