DNA-3-methyladenine glycosylase - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Contribute

Send feedback

Read comments (?) or add your own

P22134 (MAG_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 116. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

text xml rdf/xml gff fasta

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
DNA-3-methyladenine glycosylase
EC=3.2.2.21

Alternative name(s):
3-methyladenine DNA glycosidase
3MEA DNA glycosylase

Gene names

Name:	MAG1
Synonyms:	MAG
Ordered Locus Names:	YER142C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	296 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine or 7-methyladenine from the damaged DNA polymer formed by alkylation lesions.
Catalytic activity	Hydrolysis of alkylated DNA, releasing 3-methyladenine, 3-methylguanine, 7-methylguanine and 7-methyladenine.
Subcellular location	Nucleus Ref.7.
Induction	By DNA damage.
Sequence similarities	Belongs to the alkylbase DNA glycosidase AlkA family.

Ontologies

Keywords
Biological process	DNA damage DNA repair
Cellular component	Nucleus
Molecular function	Hydrolase
PTM	Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	DNA dealkylation involved in DNA repair Inferred from genetic interaction Ref.2. Source: SGD base-excision repair, AP site formation Inferred from mutant phenotype PubMed 18043759. Source: SGD
Cellular_component	nucleus Inferred by curator Ref.1. Source: SGD
Molecular_function	DNA binding Inferred from electronic annotation. Source: InterPro DNA-3-methyladenine glycosylase activity Inferred from electronic annotation. Source: EC DNA-3-methylguanine glycosylase activity Inferred from electronic annotation. Source: EC DNA-7-methyladenine glycosylase activity Inferred from electronic annotation. Source: EC DNA-7-methylguanine glycosylase activity Inferred from electronic annotation. Source: EC alkylbase DNA N-glycosylase activity Inferred from direct assay Ref.1 PubMed 2682633. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CAN1	P04817	1	EBI-10381,EBI-3993

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 296

296

DNA-3-methyladenine glycosylase

PRO_0000194883

Sites

Active site

209

Proton acceptor By similarity

Site

189

Determinant for substrate specificity and/or activity By similarity

Amino acid modifications

Modified residue

110

Phosphoserine Ref.8

Sequences

Sequence

Length

Mass (Da)

Tools

P22134 [UniParc].

Last modified August 1, 1991. Version 1.
Checksum: 14CDD51CA6645507
Show »

FASTA

296

34,333

        10         20         30         40         50         60 
MKLKREYDEL IKADAVKEIA KELGSRPLEV ALPEKYIARH EEKFNMACEH ILEKDPSLFP 

        70         80         90        100        110        120 
ILKNNEFTLY LKETQVPNTL EDYFIRLAST ILSQQISGQA AESIKARVVS LYGGAFPDYK 

       130        140        150        160        170        180 
ILFEDFKDPA KCAEIAKCGL SKRKMIYLES LAVYFTEKYK DIEKLFGQKD NDEEVIESLV 

       190        200        210        220        230        240 
TNVKGIGPWS AKMFLISGLK RMDVFAPEDL GIARGFSKYL SDKPELEKEL MRERKVVKKS 

       250        260        270        280        290 
KIKHKKYNWK IYDDDIMEKC SETFSPYRSV FMFILWRLAS TNTDAMMKAE ENFVKS

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and expression in Escherichia coli of a gene for an alkylbase DNA glycosylase from Saccharomyces cerevisiae; a homologue to the bacterial alkA gene." Berdal K.G., Bjoeraas M., Bjelland S., Seeberg E.C. EMBO J. 9:4563-4568(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Saccharomyces cerevisiae 3-methyladenine DNA glycosylase has homology to the AlkA glycosylase of E. coli and is induced in response to DNA alkylation damage." Chen J., Derfler B., Samson L. EMBO J. 9:4569-4575(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome V." Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W. Davis R.W. Nature 387:78-81(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[6]	"Purification and properties of the alkylation repair DNA glycosylase encoded the MAG gene from Saccharomyces cerevisiae." Bjoras M., Klungland A., Johansen R.F., Seeberg E. Biochemistry 34:4577-4582(1995) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-11.
[7]	"Yeast mitochondrial dehydrogenases are associated in a supramolecular complex." Grandier-Vazeille X., Bathany K., Chaignepain S., Camougrand N., Manon S., Schmitter J.-M. Biochemistry 40:9758-9769(2001) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION.
[8]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-110, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X56662 Genomic DNA. Translation: CAA39989.1. X57781 Genomic DNA. Translation: CAA40927.1. U18917 Genomic DNA. Translation: AAB64669.1. AY692938 Genomic DNA. Translation: AAT92957.1. BK006939 Genomic DNA. Translation: DAA07803.1.
PIR	S12498.
RefSeq	NP_011069.1. NM_001179032.1.
3D structure databases
ProteinModelPortal	P22134.
SMR	P22134. Positions 51-291.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-6599N.
IntAct	P22134. 16 interactions.
MINT	MINT-675790.
STRING	4932.YER142C.
Proteomic databases
PaxDb	P22134.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YER142C; YER142C; YER142C.
GeneID	856885.
KEGG	sce:YER142C.
Organism-specific databases
CYGD	YER142c.
SGD	S000000944. MAG1.
Phylogenomic databases
eggNOG	COG0122.
HOGENOM	HOG000113466.
KO	K01247.
OMA	WSAKMFA.
OrthoDB	EOG45HW6S.
Gene expression databases
Genevestigator	P22134.
GermOnline	YER142C. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	1.10.1670.10. 2 hits. 1.10.340.30. 1 hit.
InterPro	IPR000035. Alkylbase_DNA_glycsylse_CS. IPR011257. DNA_glycosylase. IPR003265. HhH-GPD_domain. IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif. IPR023170. HTH_base_excis_C. [Graphical view]
Pfam	PF00730. HhH-GPD. 1 hit. [Graphical view]
SMART	SM00478. ENDO3c. 1 hit. SM00278. HhH1. 1 hit. [Graphical view]
SUPFAM	SSF48150. DNA_glycsylse. 1 hit.
PROSITE	PS00516. ALKYLBASE_DNA_GLYCOS. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	983278.

Entry information

Entry name

MAG_YEAST

Accession

Primary (citable) accession number: P22134
Secondary accession number(s): D3DM49

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1991
Last sequence update:	August 1, 1991
Last modified:	May 1, 2013
This is version 116 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome V

Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Sites

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents