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P22134

- MAG_YEAST

UniProt

P22134 - MAG_YEAST

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Protein
DNA-3-methyladenine glycosylase
Gene
MAG1, MAG, YER142C
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine or 7-methyladenine from the damaged DNA polymer formed by alkylation lesions.

Catalytic activityi

Hydrolysis of alkylated DNA, releasing 3-methyladenine, 3-methylguanine, 7-methylguanine and 7-methyladenine.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei189 – 1891Determinant for substrate specificity and/or activity By similarity
Active sitei209 – 2091Proton acceptor By similarity

GO - Molecular functioni

  1. DNA-3-methyladenine glycosylase activity Source: SGD
  2. DNA-3-methylguanine glycosylase activity Source: UniProtKB-EC
  3. DNA-7-methyladenine glycosylase activity Source: UniProtKB-EC
  4. DNA-7-methylguanine glycosylase activity Source: UniProtKB-EC
  5. alkylbase DNA N-glycosylase activity Source: SGD
  6. damaged DNA binding Source: SGD

GO - Biological processi

  1. DNA dealkylation involved in DNA repair Source: SGD
  2. base-excision repair, AP site formation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

DNA damage, DNA repair

Enzyme and pathway databases

BioCyciYEAST:G3O-30303-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-3-methyladenine glycosylase (EC:3.2.2.21)
Alternative name(s):
3-methyladenine DNA glycosidase
3MEA DNA glycosylase
Gene namesi
Name:MAG1
Synonyms:MAG
Ordered Locus Names:YER142C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome V

Organism-specific databases

CYGDiYER142c.
SGDiS000000944. MAG1.

Subcellular locationi

Nucleus 1 Publication

GO - Cellular componenti

  1. nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 296296DNA-3-methyladenine glycosylase
PRO_0000194883Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei110 – 1101Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP22134.
PaxDbiP22134.

Expressioni

Inductioni

By DNA damage.

Gene expression databases

GenevestigatoriP22134.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
CAN1P048171EBI-10381,EBI-3993

Protein-protein interaction databases

BioGridi36891. 72 interactions.
DIPiDIP-6599N.
MINTiMINT-675790.
STRINGi4932.YER142C.

Structurei

3D structure databases

ProteinModelPortaliP22134.
SMRiP22134. Positions 51-291.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0122.
HOGENOMiHOG000113466.
KOiK01247.
OMAiWSAKMFA.
OrthoDBiEOG78SQVJ.

Family and domain databases

Gene3Di1.10.1670.10. 2 hits.
1.10.340.30. 1 hit.
InterProiIPR000035. Alkylbase_DNA_glycsylse_CS.
IPR011257. DNA_glycosylase.
IPR003265. HhH-GPD_domain.
IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif.
IPR023170. HTH_base_excis_C.
[Graphical view]
PfamiPF00730. HhH-GPD. 1 hit.
[Graphical view]
SMARTiSM00478. ENDO3c. 1 hit.
SM00278. HhH1. 1 hit.
[Graphical view]
SUPFAMiSSF48150. SSF48150. 2 hits.
PROSITEiPS00516. ALKYLBASE_DNA_GLYCOS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P22134-1 [UniParc]FASTAAdd to Basket

« Hide

MKLKREYDEL IKADAVKEIA KELGSRPLEV ALPEKYIARH EEKFNMACEH    50
ILEKDPSLFP ILKNNEFTLY LKETQVPNTL EDYFIRLAST ILSQQISGQA 100
AESIKARVVS LYGGAFPDYK ILFEDFKDPA KCAEIAKCGL SKRKMIYLES 150
LAVYFTEKYK DIEKLFGQKD NDEEVIESLV TNVKGIGPWS AKMFLISGLK 200
RMDVFAPEDL GIARGFSKYL SDKPELEKEL MRERKVVKKS KIKHKKYNWK 250
IYDDDIMEKC SETFSPYRSV FMFILWRLAS TNTDAMMKAE ENFVKS 296
Length:296
Mass (Da):34,333
Last modified:August 1, 1991 - v1
Checksum:i14CDD51CA6645507
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X56662 Genomic DNA. Translation: CAA39989.1.
X57781 Genomic DNA. Translation: CAA40927.1.
U18917 Genomic DNA. Translation: AAB64669.1.
AY692938 Genomic DNA. Translation: AAT92957.1.
BK006939 Genomic DNA. Translation: DAA07803.1.
PIRiS12498.
RefSeqiNP_011069.1. NM_001179032.1.

Genome annotation databases

EnsemblFungiiYER142C; YER142C; YER142C.
GeneIDi856885.
KEGGisce:YER142C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X56662 Genomic DNA. Translation: CAA39989.1 .
X57781 Genomic DNA. Translation: CAA40927.1 .
U18917 Genomic DNA. Translation: AAB64669.1 .
AY692938 Genomic DNA. Translation: AAT92957.1 .
BK006939 Genomic DNA. Translation: DAA07803.1 .
PIRi S12498.
RefSeqi NP_011069.1. NM_001179032.1.

3D structure databases

ProteinModelPortali P22134.
SMRi P22134. Positions 51-291.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 36891. 72 interactions.
DIPi DIP-6599N.
MINTi MINT-675790.
STRINGi 4932.YER142C.

Proteomic databases

MaxQBi P22134.
PaxDbi P22134.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YER142C ; YER142C ; YER142C .
GeneIDi 856885.
KEGGi sce:YER142C.

Organism-specific databases

CYGDi YER142c.
SGDi S000000944. MAG1.

Phylogenomic databases

eggNOGi COG0122.
HOGENOMi HOG000113466.
KOi K01247.
OMAi WSAKMFA.
OrthoDBi EOG78SQVJ.

Enzyme and pathway databases

BioCyci YEAST:G3O-30303-MONOMER.

Miscellaneous databases

NextBioi 983278.

Gene expression databases

Genevestigatori P22134.

Family and domain databases

Gene3Di 1.10.1670.10. 2 hits.
1.10.340.30. 1 hit.
InterProi IPR000035. Alkylbase_DNA_glycsylse_CS.
IPR011257. DNA_glycosylase.
IPR003265. HhH-GPD_domain.
IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif.
IPR023170. HTH_base_excis_C.
[Graphical view ]
Pfami PF00730. HhH-GPD. 1 hit.
[Graphical view ]
SMARTi SM00478. ENDO3c. 1 hit.
SM00278. HhH1. 1 hit.
[Graphical view ]
SUPFAMi SSF48150. SSF48150. 2 hits.
PROSITEi PS00516. ALKYLBASE_DNA_GLYCOS. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and expression in Escherichia coli of a gene for an alkylbase DNA glycosylase from Saccharomyces cerevisiae; a homologue to the bacterial alkA gene."
    Berdal K.G., Bjoeraas M., Bjelland S., Seeberg E.C.
    EMBO J. 9:4563-4568(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Saccharomyces cerevisiae 3-methyladenine DNA glycosylase has homology to the AlkA glycosylase of E. coli and is induced in response to DNA alkylation damage."
    Chen J., Derfler B., Samson L.
    EMBO J. 9:4569-4575(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  6. "Purification and properties of the alkylation repair DNA glycosylase encoded the MAG gene from Saccharomyces cerevisiae."
    Bjoras M., Klungland A., Johansen R.F., Seeberg E.
    Biochemistry 34:4577-4582(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 1-11.
  7. "Yeast mitochondrial dehydrogenases are associated in a supramolecular complex."
    Grandier-Vazeille X., Bathany K., Chaignepain S., Camougrand N., Manon S., Schmitter J.-M.
    Biochemistry 40:9758-9769(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  8. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-110, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiMAG_YEAST
AccessioniPrimary (citable) accession number: P22134
Secondary accession number(s): D3DM49
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: August 1, 1991
Last modified: June 11, 2014
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome V
    Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

External Data

Dasty 3

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