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P22134 (MAG_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 125. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA-3-methyladenine glycosylase

EC=3.2.2.21
Alternative name(s):
3-methyladenine DNA glycosidase
3MEA DNA glycosylase
Gene names
Name:MAG1
Synonyms:MAG
Ordered Locus Names:YER142C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length296 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine or 7-methyladenine from the damaged DNA polymer formed by alkylation lesions.

Catalytic activity

Hydrolysis of alkylated DNA, releasing 3-methyladenine, 3-methylguanine, 7-methylguanine and 7-methyladenine.

Subcellular location

Nucleus Ref.7.

Induction

By DNA damage.

Sequence similarities

Belongs to the alkylbase DNA glycosidase AlkA family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

CAN1P048171EBI-10381,EBI-3993

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 296296DNA-3-methyladenine glycosylase
PRO_0000194883

Sites

Active site2091Proton acceptor By similarity
Site1891Determinant for substrate specificity and/or activity By similarity

Amino acid modifications

Modified residue1101Phosphoserine Ref.8

Sequences

Sequence LengthMass (Da)Tools
P22134 [UniParc].

Last modified August 1, 1991. Version 1.
Checksum: 14CDD51CA6645507

FASTA29634,333
        10         20         30         40         50         60 
MKLKREYDEL IKADAVKEIA KELGSRPLEV ALPEKYIARH EEKFNMACEH ILEKDPSLFP 

        70         80         90        100        110        120 
ILKNNEFTLY LKETQVPNTL EDYFIRLAST ILSQQISGQA AESIKARVVS LYGGAFPDYK 

       130        140        150        160        170        180 
ILFEDFKDPA KCAEIAKCGL SKRKMIYLES LAVYFTEKYK DIEKLFGQKD NDEEVIESLV 

       190        200        210        220        230        240 
TNVKGIGPWS AKMFLISGLK RMDVFAPEDL GIARGFSKYL SDKPELEKEL MRERKVVKKS 

       250        260        270        280        290 
KIKHKKYNWK IYDDDIMEKC SETFSPYRSV FMFILWRLAS TNTDAMMKAE ENFVKS 

« Hide

References

« Hide 'large scale' references
[1]"Cloning and expression in Escherichia coli of a gene for an alkylbase DNA glycosylase from Saccharomyces cerevisiae; a homologue to the bacterial alkA gene."
Berdal K.G., Bjoeraas M., Bjelland S., Seeberg E.C.
EMBO J. 9:4563-4568(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Saccharomyces cerevisiae 3-methyladenine DNA glycosylase has homology to the AlkA glycosylase of E. coli and is induced in response to DNA alkylation damage."
Chen J., Derfler B., Samson L.
EMBO J. 9:4569-4575(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"The nucleotide sequence of Saccharomyces cerevisiae chromosome V."
Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W. expand/collapse author list , Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X., Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y., Botstein D., Davis R.W.
Nature 387:78-81(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[6]"Purification and properties of the alkylation repair DNA glycosylase encoded the MAG gene from Saccharomyces cerevisiae."
Bjoras M., Klungland A., Johansen R.F., Seeberg E.
Biochemistry 34:4577-4582(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 1-11.
[7]"Yeast mitochondrial dehydrogenases are associated in a supramolecular complex."
Grandier-Vazeille X., Bathany K., Chaignepain S., Camougrand N., Manon S., Schmitter J.-M.
Biochemistry 40:9758-9769(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[8]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-110, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X56662 Genomic DNA. Translation: CAA39989.1.
X57781 Genomic DNA. Translation: CAA40927.1.
U18917 Genomic DNA. Translation: AAB64669.1.
AY692938 Genomic DNA. Translation: AAT92957.1.
BK006939 Genomic DNA. Translation: DAA07803.1.
PIRS12498.
RefSeqNP_011069.1. NM_001179032.1.

3D structure databases

ProteinModelPortalP22134.
SMRP22134. Positions 51-291.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid36891. 71 interactions.
DIPDIP-6599N.
MINTMINT-675790.
STRING4932.YER142C.

Proteomic databases

PaxDbP22134.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYER142C; YER142C; YER142C.
GeneID856885.
KEGGsce:YER142C.

Organism-specific databases

CYGDYER142c.
SGDS000000944. MAG1.

Phylogenomic databases

eggNOGCOG0122.
HOGENOMHOG000113466.
KOK01247.
OMAWSAKMFA.
OrthoDBEOG78SQVJ.

Enzyme and pathway databases

BioCycYEAST:G3O-30303-MONOMER.

Gene expression databases

GenevestigatorP22134.

Family and domain databases

Gene3D1.10.1670.10. 2 hits.
1.10.340.30. 1 hit.
InterProIPR000035. Alkylbase_DNA_glycsylse_CS.
IPR011257. DNA_glycosylase.
IPR003265. HhH-GPD_domain.
IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif.
IPR023170. HTH_base_excis_C.
[Graphical view]
PfamPF00730. HhH-GPD. 1 hit.
[Graphical view]
SMARTSM00478. ENDO3c. 1 hit.
SM00278. HhH1. 1 hit.
[Graphical view]
SUPFAMSSF48150. SSF48150. 2 hits.
PROSITEPS00516. ALKYLBASE_DNA_GLYCOS. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio983278.

Entry information

Entry nameMAG_YEAST
AccessionPrimary (citable) accession number: P22134
Secondary accession number(s): D3DM49
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: August 1, 1991
Last modified: March 19, 2014
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome V

Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families