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P22134

- MAG_YEAST

UniProt

P22134 - MAG_YEAST

Protein

DNA-3-methyladenine glycosylase

Gene

MAG1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 128 (01 Oct 2014)
      Sequence version 1 (01 Aug 1991)
      Previous versions | rss
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    Functioni

    Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine or 7-methyladenine from the damaged DNA polymer formed by alkylation lesions.

    Catalytic activityi

    Hydrolysis of alkylated DNA, releasing 3-methyladenine, 3-methylguanine, 7-methylguanine and 7-methyladenine.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei189 – 1891Determinant for substrate specificity and/or activityBy similarity
    Active sitei209 – 2091Proton acceptorBy similarity

    GO - Molecular functioni

    1. alkylbase DNA N-glycosylase activity Source: SGD
    2. damaged DNA binding Source: SGD
    3. DNA-3-methyladenine glycosylase activity Source: SGD
    4. DNA-3-methylguanine glycosylase activity Source: UniProtKB-EC
    5. DNA-7-methyladenine glycosylase activity Source: UniProtKB-EC
    6. DNA-7-methylguanine glycosylase activity Source: UniProtKB-EC

    GO - Biological processi

    1. base-excision repair, AP site formation Source: SGD
    2. DNA dealkylation involved in DNA repair Source: SGD

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    DNA damage, DNA repair

    Enzyme and pathway databases

    BioCyciYEAST:G3O-30303-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA-3-methyladenine glycosylase (EC:3.2.2.21)
    Alternative name(s):
    3-methyladenine DNA glycosidase
    3MEA DNA glycosylase
    Gene namesi
    Name:MAG1
    Synonyms:MAG
    Ordered Locus Names:YER142C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome V

    Organism-specific databases

    CYGDiYER142c.
    SGDiS000000944. MAG1.

    Subcellular locationi

    Nucleus 1 Publication

    GO - Cellular componenti

    1. nucleus Source: SGD

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 296296DNA-3-methyladenine glycosylasePRO_0000194883Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei110 – 1101Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP22134.
    PaxDbiP22134.

    Expressioni

    Inductioni

    By DNA damage.

    Gene expression databases

    GenevestigatoriP22134.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    CAN1P048171EBI-10381,EBI-3993

    Protein-protein interaction databases

    BioGridi36891. 72 interactions.
    DIPiDIP-6599N.
    MINTiMINT-675790.
    STRINGi4932.YER142C.

    Structurei

    3D structure databases

    ProteinModelPortaliP22134.
    SMRiP22134. Positions 51-291.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0122.
    HOGENOMiHOG000113466.
    KOiK01247.
    OMAiWSAKMFA.
    OrthoDBiEOG78SQVJ.

    Family and domain databases

    Gene3Di1.10.1670.10. 2 hits.
    1.10.340.30. 1 hit.
    InterProiIPR000035. Alkylbase_DNA_glycsylse_CS.
    IPR011257. DNA_glycosylase.
    IPR003265. HhH-GPD_domain.
    IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif.
    IPR023170. HTH_base_excis_C.
    [Graphical view]
    PfamiPF00730. HhH-GPD. 1 hit.
    [Graphical view]
    SMARTiSM00478. ENDO3c. 1 hit.
    SM00278. HhH1. 1 hit.
    [Graphical view]
    SUPFAMiSSF48150. SSF48150. 2 hits.
    PROSITEiPS00516. ALKYLBASE_DNA_GLYCOS. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P22134-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKLKREYDEL IKADAVKEIA KELGSRPLEV ALPEKYIARH EEKFNMACEH    50
    ILEKDPSLFP ILKNNEFTLY LKETQVPNTL EDYFIRLAST ILSQQISGQA 100
    AESIKARVVS LYGGAFPDYK ILFEDFKDPA KCAEIAKCGL SKRKMIYLES 150
    LAVYFTEKYK DIEKLFGQKD NDEEVIESLV TNVKGIGPWS AKMFLISGLK 200
    RMDVFAPEDL GIARGFSKYL SDKPELEKEL MRERKVVKKS KIKHKKYNWK 250
    IYDDDIMEKC SETFSPYRSV FMFILWRLAS TNTDAMMKAE ENFVKS 296
    Length:296
    Mass (Da):34,333
    Last modified:August 1, 1991 - v1
    Checksum:i14CDD51CA6645507
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X56662 Genomic DNA. Translation: CAA39989.1.
    X57781 Genomic DNA. Translation: CAA40927.1.
    U18917 Genomic DNA. Translation: AAB64669.1.
    AY692938 Genomic DNA. Translation: AAT92957.1.
    BK006939 Genomic DNA. Translation: DAA07803.1.
    PIRiS12498.
    RefSeqiNP_011069.1. NM_001179032.1.

    Genome annotation databases

    EnsemblFungiiYER142C; YER142C; YER142C.
    GeneIDi856885.
    KEGGisce:YER142C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X56662 Genomic DNA. Translation: CAA39989.1 .
    X57781 Genomic DNA. Translation: CAA40927.1 .
    U18917 Genomic DNA. Translation: AAB64669.1 .
    AY692938 Genomic DNA. Translation: AAT92957.1 .
    BK006939 Genomic DNA. Translation: DAA07803.1 .
    PIRi S12498.
    RefSeqi NP_011069.1. NM_001179032.1.

    3D structure databases

    ProteinModelPortali P22134.
    SMRi P22134. Positions 51-291.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 36891. 72 interactions.
    DIPi DIP-6599N.
    MINTi MINT-675790.
    STRINGi 4932.YER142C.

    Proteomic databases

    MaxQBi P22134.
    PaxDbi P22134.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YER142C ; YER142C ; YER142C .
    GeneIDi 856885.
    KEGGi sce:YER142C.

    Organism-specific databases

    CYGDi YER142c.
    SGDi S000000944. MAG1.

    Phylogenomic databases

    eggNOGi COG0122.
    HOGENOMi HOG000113466.
    KOi K01247.
    OMAi WSAKMFA.
    OrthoDBi EOG78SQVJ.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-30303-MONOMER.

    Miscellaneous databases

    NextBioi 983278.

    Gene expression databases

    Genevestigatori P22134.

    Family and domain databases

    Gene3Di 1.10.1670.10. 2 hits.
    1.10.340.30. 1 hit.
    InterProi IPR000035. Alkylbase_DNA_glycsylse_CS.
    IPR011257. DNA_glycosylase.
    IPR003265. HhH-GPD_domain.
    IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif.
    IPR023170. HTH_base_excis_C.
    [Graphical view ]
    Pfami PF00730. HhH-GPD. 1 hit.
    [Graphical view ]
    SMARTi SM00478. ENDO3c. 1 hit.
    SM00278. HhH1. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48150. SSF48150. 2 hits.
    PROSITEi PS00516. ALKYLBASE_DNA_GLYCOS. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and expression in Escherichia coli of a gene for an alkylbase DNA glycosylase from Saccharomyces cerevisiae; a homologue to the bacterial alkA gene."
      Berdal K.G., Bjoeraas M., Bjelland S., Seeberg E.C.
      EMBO J. 9:4563-4568(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Saccharomyces cerevisiae 3-methyladenine DNA glycosylase has homology to the AlkA glycosylase of E. coli and is induced in response to DNA alkylation damage."
      Chen J., Derfler B., Samson L.
      EMBO J. 9:4569-4575(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    6. "Purification and properties of the alkylation repair DNA glycosylase encoded the MAG gene from Saccharomyces cerevisiae."
      Bjoras M., Klungland A., Johansen R.F., Seeberg E.
      Biochemistry 34:4577-4582(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 1-11.
    7. "Yeast mitochondrial dehydrogenases are associated in a supramolecular complex."
      Grandier-Vazeille X., Bathany K., Chaignepain S., Camougrand N., Manon S., Schmitter J.-M.
      Biochemistry 40:9758-9769(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
    8. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-110, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiMAG_YEAST
    AccessioniPrimary (citable) accession number: P22134
    Secondary accession number(s): D3DM49
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1991
    Last sequence update: August 1, 1991
    Last modified: October 1, 2014
    This is version 128 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome V
      Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

    External Data

    Dasty 3