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Protein

DNA-3-methyladenine glycosylase

Gene

MAG1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine or 7-methyladenine from the damaged DNA polymer formed by alkylation lesions.

Catalytic activityi

Hydrolysis of alkylated DNA, releasing 3-methyladenine, 3-methylguanine, 7-methylguanine and 7-methyladenine.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei189Determinant for substrate specificity and/or activityBy similarity1
Active sitei209Proton acceptorBy similarity1

GO - Molecular functioni

GO - Biological processi

  • base-excision repair, AP site formation Source: SGD
  • DNA dealkylation involved in DNA repair Source: SGD

Keywordsi

Molecular functionHydrolase
Biological processDNA damage, DNA repair

Enzyme and pathway databases

BioCyciYEAST:G3O-30303-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-3-methyladenine glycosylase (EC:3.2.2.21)
Alternative name(s):
3-methyladenine DNA glycosidase
3MEA DNA glycosylase
Gene namesi
Name:MAG1
Synonyms:MAG
Ordered Locus Names:YER142C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome V

Organism-specific databases

EuPathDBiFungiDB:YER142C.
SGDiS000000944. MAG1.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: SGD
  • protein-DNA complex Source: GO_Central

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001948831 – 296DNA-3-methyladenine glycosylaseAdd BLAST296

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei110PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP22134.
PRIDEiP22134.

PTM databases

iPTMnetiP22134.

Expressioni

Inductioni

By DNA damage.

Interactioni

Protein-protein interaction databases

BioGridi36891. 114 interactors.
DIPiDIP-6599N.
MINTiMINT-675790.

Structurei

3D structure databases

ProteinModelPortaliP22134.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000113466.
InParanoidiP22134.
KOiK01247.
OMAiWSAKMFA.
OrthoDBiEOG092C51YL.

Family and domain databases

CDDicd00056. ENDO3c. 1 hit.
Gene3Di1.10.1670.10. 2 hits.
1.10.340.30. 1 hit.
InterProiView protein in InterPro
IPR000035. Alkylbase_DNA_glycsylse_CS.
IPR011257. DNA_glycosylase.
IPR003265. HhH-GPD_domain.
IPR023170. HTH_base_excis_C.
PfamiView protein in Pfam
PF00730. HhH-GPD. 1 hit.
SMARTiView protein in SMART
SM00478. ENDO3c. 1 hit.
SUPFAMiSSF48150. SSF48150. 2 hits.
PROSITEiView protein in PROSITE
PS00516. ALKYLBASE_DNA_GLYCOS. 1 hit.

Sequencei

Sequence statusi: Complete.

P22134-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLKREYDEL IKADAVKEIA KELGSRPLEV ALPEKYIARH EEKFNMACEH
60 70 80 90 100
ILEKDPSLFP ILKNNEFTLY LKETQVPNTL EDYFIRLAST ILSQQISGQA
110 120 130 140 150
AESIKARVVS LYGGAFPDYK ILFEDFKDPA KCAEIAKCGL SKRKMIYLES
160 170 180 190 200
LAVYFTEKYK DIEKLFGQKD NDEEVIESLV TNVKGIGPWS AKMFLISGLK
210 220 230 240 250
RMDVFAPEDL GIARGFSKYL SDKPELEKEL MRERKVVKKS KIKHKKYNWK
260 270 280 290
IYDDDIMEKC SETFSPYRSV FMFILWRLAS TNTDAMMKAE ENFVKS
Length:296
Mass (Da):34,333
Last modified:August 1, 1991 - v1
Checksum:i14CDD51CA6645507
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X56662 Genomic DNA. Translation: CAA39989.1.
X57781 Genomic DNA. Translation: CAA40927.1.
U18917 Genomic DNA. Translation: AAB64669.1.
AY692938 Genomic DNA. Translation: AAT92957.1.
BK006939 Genomic DNA. Translation: DAA07803.1.
PIRiS12498.
RefSeqiNP_011069.1. NM_001179032.1.

Genome annotation databases

EnsemblFungiiYER142C; YER142C; YER142C.
GeneIDi856885.
KEGGisce:YER142C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X56662 Genomic DNA. Translation: CAA39989.1.
X57781 Genomic DNA. Translation: CAA40927.1.
U18917 Genomic DNA. Translation: AAB64669.1.
AY692938 Genomic DNA. Translation: AAT92957.1.
BK006939 Genomic DNA. Translation: DAA07803.1.
PIRiS12498.
RefSeqiNP_011069.1. NM_001179032.1.

3D structure databases

ProteinModelPortaliP22134.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36891. 114 interactors.
DIPiDIP-6599N.
MINTiMINT-675790.

PTM databases

iPTMnetiP22134.

Proteomic databases

MaxQBiP22134.
PRIDEiP22134.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYER142C; YER142C; YER142C.
GeneIDi856885.
KEGGisce:YER142C.

Organism-specific databases

EuPathDBiFungiDB:YER142C.
SGDiS000000944. MAG1.

Phylogenomic databases

HOGENOMiHOG000113466.
InParanoidiP22134.
KOiK01247.
OMAiWSAKMFA.
OrthoDBiEOG092C51YL.

Enzyme and pathway databases

BioCyciYEAST:G3O-30303-MONOMER.

Miscellaneous databases

PROiP22134.

Family and domain databases

CDDicd00056. ENDO3c. 1 hit.
Gene3Di1.10.1670.10. 2 hits.
1.10.340.30. 1 hit.
InterProiView protein in InterPro
IPR000035. Alkylbase_DNA_glycsylse_CS.
IPR011257. DNA_glycosylase.
IPR003265. HhH-GPD_domain.
IPR023170. HTH_base_excis_C.
PfamiView protein in Pfam
PF00730. HhH-GPD. 1 hit.
SMARTiView protein in SMART
SM00478. ENDO3c. 1 hit.
SUPFAMiSSF48150. SSF48150. 2 hits.
PROSITEiView protein in PROSITE
PS00516. ALKYLBASE_DNA_GLYCOS. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMAG_YEAST
AccessioniPrimary (citable) accession number: P22134
Secondary accession number(s): D3DM49
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: August 1, 1991
Last modified: March 15, 2017
This is version 149 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome V
    Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.