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P22133

- MDHC_YEAST

UniProt

P22133 - MDHC_YEAST

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Protein

Malate dehydrogenase, cytoplasmic

Gene

MDH2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

The isoenzyme MDH2 may function primarily in the glyoxylate cycle.

Catalytic activityi

(S)-malate + NAD+ = oxaloacetate + NADH.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei57 – 571NADBy similarity
Binding sitei106 – 1061SubstratePROSITE-ProRule annotation
Binding sitei112 – 1121SubstratePROSITE-ProRule annotation
Binding sitei119 – 1191NADBy similarity
Binding sitei146 – 1461SubstratePROSITE-ProRule annotation
Binding sitei185 – 1851SubstratePROSITE-ProRule annotation
Active sitei215 – 2151Proton acceptorBy similarity
Binding sitei266 – 2661NADBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi20 – 267NADBy similarity
Nucleotide bindingi144 – 1463NADBy similarity

GO - Molecular functioni

  1. L-malate dehydrogenase activity Source: SGD

GO - Biological processi

  1. cellular carbohydrate metabolic process Source: InterPro
  2. gluconeogenesis Source: SGD
  3. malate metabolic process Source: SGD
  4. protein import into peroxisome matrix Source: SGD
  5. tricarboxylic acid cycle Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Tricarboxylic acid cycle

Keywords - Ligandi

NAD

Enzyme and pathway databases

BioCyciYEAST:YOL126C-MONOMER.
ReactomeiREACT_235985. Gluconeogenesis.
REACT_245486. Citric acid cycle (TCA cycle).
SABIO-RKP22133.

Names & Taxonomyi

Protein namesi
Recommended name:
Malate dehydrogenase, cytoplasmic (EC:1.1.1.37)
Gene namesi
Name:MDH2
Ordered Locus Names:YOL126C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XV

Organism-specific databases

SGDiS000005486. MDH2.

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed2 Publications
Chaini2 – 377376Malate dehydrogenase, cytoplasmicPRO_0000113338Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei6 – 61Phosphothreonine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP22133.
PaxDbiP22133.
PeptideAtlasiP22133.

Expressioni

Inductioni

By acetate as carbon source in the growth medium. Is inactivated by addition of glucose (catabolite inactivation).

Gene expression databases

GenevestigatoriP22133.

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

BioGridi34249. 39 interactions.
DIPiDIP-4211N.
IntActiP22133. 6 interactions.
MINTiMINT-568274.
STRINGi4932.YOL126C.

Structurei

3D structure databases

ProteinModelPortaliP22133.
SMRiP22133. Positions 14-370.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. MDH type 1 family.Curated

Phylogenomic databases

eggNOGiCOG0039.
GeneTreeiENSGT00550000075847.
HOGENOMiHOG000213792.
InParanoidiP22133.
KOiK00026.
OMAiETIIPLF.
OrthoDBiEOG7JDR71.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
InterProiIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR001252. Malate_DH_AS.
IPR010097. Malate_DH_type1.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11540. PTHR11540. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
SUPFAMiSSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01772. MDH_euk_gproteo. 1 hit.
PROSITEiPS00068. MDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P22133-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPHSVTPSIE QDSLKIAILG AAGGIGQSLS LLLKAQLQYQ LKESNRSVTH
60 70 80 90 100
IHLALYDVNQ EAINGVTADL SHIDTPISVS SHSPAGGIEN CLHNASIVVI
110 120 130 140 150
PAGVPRKPGM TRDDLFNVNA GIISQLGDSI AECCDLSKVF VLVISNPVNS
160 170 180 190 200
LVPVMVSNIL KNHPQSRNSG IERRIMGVTK LDIVRASTFL REINIESGLT
210 220 230 240 250
PRVNSMPDVP VIGGHSGETI IPLFSQSNFL SRLNEDQLKY LIHRVQYGGD
260 270 280 290 300
EVVKAKNGKG SATLSMAHAG YKCVVQFVSL LLGNIEQIHG TYYVPLKDAN
310 320 330 340 350
NFPIAPGADQ LLPLVDGADY FAIPLTITTK GVSYVDYDIV NRMNDMERNQ
360 370
MLPICVSQLK KNIDKGLEFV ASRSASS
Length:377
Mass (Da):40,731
Last modified:January 23, 2007 - v2
Checksum:iCAF4784FA7DD6E27
GO

Sequence cautioni

The sequence AAC49466.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence CAA99145.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M62808 Genomic DNA. Translation: AAA34766.1.
U41293 Genomic DNA. Translation: AAC49466.1. Different initiation.
Z74868 Genomic DNA. Translation: CAA99145.1. Different initiation.
BK006948 Genomic DNA. Translation: DAA10658.1.
PIRiS63444. DEBYMC.
RefSeqiNP_014515.2. NM_001183380.1.

Genome annotation databases

EnsemblFungiiYOL126C; YOL126C; YOL126C.
GeneIDi853994.
KEGGisce:YOL126C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M62808 Genomic DNA. Translation: AAA34766.1 .
U41293 Genomic DNA. Translation: AAC49466.1 . Different initiation.
Z74868 Genomic DNA. Translation: CAA99145.1 . Different initiation.
BK006948 Genomic DNA. Translation: DAA10658.1 .
PIRi S63444. DEBYMC.
RefSeqi NP_014515.2. NM_001183380.1.

3D structure databases

ProteinModelPortali P22133.
SMRi P22133. Positions 14-370.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 34249. 39 interactions.
DIPi DIP-4211N.
IntActi P22133. 6 interactions.
MINTi MINT-568274.
STRINGi 4932.YOL126C.

Proteomic databases

MaxQBi P22133.
PaxDbi P22133.
PeptideAtlasi P22133.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YOL126C ; YOL126C ; YOL126C .
GeneIDi 853994.
KEGGi sce:YOL126C.

Organism-specific databases

SGDi S000005486. MDH2.

Phylogenomic databases

eggNOGi COG0039.
GeneTreei ENSGT00550000075847.
HOGENOMi HOG000213792.
InParanoidi P22133.
KOi K00026.
OMAi ETIIPLF.
OrthoDBi EOG7JDR71.

Enzyme and pathway databases

BioCyci YEAST:YOL126C-MONOMER.
Reactomei REACT_235985. Gluconeogenesis.
REACT_245486. Citric acid cycle (TCA cycle).
SABIO-RK P22133.

Miscellaneous databases

NextBioi 975482.

Gene expression databases

Genevestigatori P22133.

Family and domain databases

Gene3Di 3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
InterProi IPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR001252. Malate_DH_AS.
IPR010097. Malate_DH_type1.
IPR016040. NAD(P)-bd_dom.
[Graphical view ]
PANTHERi PTHR11540. PTHR11540. 1 hit.
Pfami PF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view ]
PIRSFi PIRSF000102. Lac_mal_DH. 1 hit.
SUPFAMi SSF56327. SSF56327. 1 hit.
TIGRFAMsi TIGR01772. MDH_euk_gproteo. 1 hit.
PROSITEi PS00068. MDH. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation, nucleotide sequence analysis, and disruption of the MDH2 gene from Saccharomyces cerevisiae: evidence for three isozymes of yeast malate dehydrogenase."
    Minard K.I., McAlister-Henn L.
    Mol. Cell. Biol. 11:370-380(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-17.
  2. "Sequence analysis of a 13.4 kbp fragment from the left arm of chromosome XV reveals a malate dehydrogenase gene, a putative Ser/Thr protein kinase, the ribosomal L25 gene and four new open reading frames."
    Casamayor A., Khalid H., Balcells L., Aldea M., Casas C., Herrero E., Arino J.
    Yeast 12:1013-1020(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 96604 / S288c / FY1679.
  3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
    Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D.
    , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
    Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. "Purification procedure and N-terminal amino acid sequence of yeast malate dehydrogenase isoenzymes."
    Kopetzki E., Entian K.-D., Lottspeich F., Mecke D.
    Biochim. Biophys. Acta 912:398-403(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-35.
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-6, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiMDHC_YEAST
AccessioniPrimary (citable) accession number: P22133
Secondary accession number(s): D6W1U2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: January 23, 2007
Last modified: November 26, 2014
This is version 140 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Yeast contains at least 3 malate dehydrogenase isoenzymes: a mitochondrial (MDH1), a cytoplasmic (MDH2) and a peroxisomal (MDH3).
Present with 5260 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

External Data

Dasty 3