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P22133

- MDHC_YEAST

UniProt

P22133 - MDHC_YEAST

Protein

Malate dehydrogenase, cytoplasmic

Gene

MDH2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 138 (01 Oct 2014)
      Sequence version 2 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    The isoenzyme MDH2 may function primarily in the glyoxylate cycle.

    Catalytic activityi

    (S)-malate + NAD+ = oxaloacetate + NADH.PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei57 – 571NADBy similarity
    Binding sitei106 – 1061SubstratePROSITE-ProRule annotation
    Binding sitei112 – 1121SubstratePROSITE-ProRule annotation
    Binding sitei119 – 1191NADBy similarity
    Binding sitei146 – 1461SubstratePROSITE-ProRule annotation
    Binding sitei185 – 1851SubstratePROSITE-ProRule annotation
    Active sitei215 – 2151Proton acceptorBy similarity
    Binding sitei266 – 2661NADBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi20 – 267NADBy similarity
    Nucleotide bindingi144 – 1463NADBy similarity

    GO - Molecular functioni

    1. L-malate dehydrogenase activity Source: SGD

    GO - Biological processi

    1. cellular carbohydrate metabolic process Source: InterPro
    2. gluconeogenesis Source: SGD
    3. malate metabolic process Source: SGD
    4. protein import into peroxisome matrix Source: SGD
    5. tricarboxylic acid cycle Source: UniProtKB-KW

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Tricarboxylic acid cycle

    Keywords - Ligandi

    NAD

    Enzyme and pathway databases

    BioCyciYEAST:YOL126C-MONOMER.
    SABIO-RKP22133.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Malate dehydrogenase, cytoplasmic (EC:1.1.1.37)
    Gene namesi
    Name:MDH2
    Ordered Locus Names:YOL126C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XV

    Organism-specific databases

    SGDiS000005486. MDH2.

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: SGD

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed2 Publications
    Chaini2 – 377376Malate dehydrogenase, cytoplasmicPRO_0000113338Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei6 – 61Phosphothreonine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP22133.
    PaxDbiP22133.
    PeptideAtlasiP22133.

    Expressioni

    Inductioni

    By acetate as carbon source in the growth medium. Is inactivated by addition of glucose (catabolite inactivation).

    Gene expression databases

    GenevestigatoriP22133.

    Interactioni

    Subunit structurei

    Homodimer.

    Protein-protein interaction databases

    BioGridi34249. 38 interactions.
    DIPiDIP-4211N.
    IntActiP22133. 6 interactions.
    MINTiMINT-568274.
    STRINGi4932.YOL126C.

    Structurei

    3D structure databases

    ProteinModelPortaliP22133.
    SMRiP22133. Positions 14-370.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the LDH/MDH superfamily. MDH type 1 family.Curated

    Phylogenomic databases

    eggNOGiCOG0039.
    GeneTreeiENSGT00550000075847.
    HOGENOMiHOG000213792.
    KOiK00026.
    OMAiETIIPLF.
    OrthoDBiEOG7JDR71.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    3.90.110.10. 1 hit.
    InterProiIPR001557. L-lactate/malate_DH.
    IPR022383. Lactate/malate_DH_C.
    IPR001236. Lactate/malate_DH_N.
    IPR015955. Lactate_DH/Glyco_Ohase_4_C.
    IPR001252. Malate_DH_AS.
    IPR010097. Malate_DH_type1.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PANTHERiPTHR11540. PTHR11540. 1 hit.
    PfamiPF02866. Ldh_1_C. 1 hit.
    PF00056. Ldh_1_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
    SUPFAMiSSF56327. SSF56327. 1 hit.
    TIGRFAMsiTIGR01772. MDH_euk_gproteo. 1 hit.
    PROSITEiPS00068. MDH. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P22133-1 [UniParc]FASTAAdd to Basket

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    MPHSVTPSIE QDSLKIAILG AAGGIGQSLS LLLKAQLQYQ LKESNRSVTH    50
    IHLALYDVNQ EAINGVTADL SHIDTPISVS SHSPAGGIEN CLHNASIVVI 100
    PAGVPRKPGM TRDDLFNVNA GIISQLGDSI AECCDLSKVF VLVISNPVNS 150
    LVPVMVSNIL KNHPQSRNSG IERRIMGVTK LDIVRASTFL REINIESGLT 200
    PRVNSMPDVP VIGGHSGETI IPLFSQSNFL SRLNEDQLKY LIHRVQYGGD 250
    EVVKAKNGKG SATLSMAHAG YKCVVQFVSL LLGNIEQIHG TYYVPLKDAN 300
    NFPIAPGADQ LLPLVDGADY FAIPLTITTK GVSYVDYDIV NRMNDMERNQ 350
    MLPICVSQLK KNIDKGLEFV ASRSASS 377
    Length:377
    Mass (Da):40,731
    Last modified:January 23, 2007 - v2
    Checksum:iCAF4784FA7DD6E27
    GO

    Sequence cautioni

    The sequence AAC49466.1 differs from that shown. Reason: Erroneous initiation.
    The sequence CAA99145.1 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M62808 Genomic DNA. Translation: AAA34766.1.
    U41293 Genomic DNA. Translation: AAC49466.1. Different initiation.
    Z74868 Genomic DNA. Translation: CAA99145.1. Different initiation.
    BK006948 Genomic DNA. Translation: DAA10658.1.
    PIRiS63444. DEBYMC.
    RefSeqiNP_014515.2. NM_001183380.1.

    Genome annotation databases

    EnsemblFungiiYOL126C; YOL126C; YOL126C.
    GeneIDi853994.
    KEGGisce:YOL126C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M62808 Genomic DNA. Translation: AAA34766.1 .
    U41293 Genomic DNA. Translation: AAC49466.1 . Different initiation.
    Z74868 Genomic DNA. Translation: CAA99145.1 . Different initiation.
    BK006948 Genomic DNA. Translation: DAA10658.1 .
    PIRi S63444. DEBYMC.
    RefSeqi NP_014515.2. NM_001183380.1.

    3D structure databases

    ProteinModelPortali P22133.
    SMRi P22133. Positions 14-370.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 34249. 38 interactions.
    DIPi DIP-4211N.
    IntActi P22133. 6 interactions.
    MINTi MINT-568274.
    STRINGi 4932.YOL126C.

    Proteomic databases

    MaxQBi P22133.
    PaxDbi P22133.
    PeptideAtlasi P22133.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YOL126C ; YOL126C ; YOL126C .
    GeneIDi 853994.
    KEGGi sce:YOL126C.

    Organism-specific databases

    SGDi S000005486. MDH2.

    Phylogenomic databases

    eggNOGi COG0039.
    GeneTreei ENSGT00550000075847.
    HOGENOMi HOG000213792.
    KOi K00026.
    OMAi ETIIPLF.
    OrthoDBi EOG7JDR71.

    Enzyme and pathway databases

    BioCyci YEAST:YOL126C-MONOMER.
    SABIO-RK P22133.

    Miscellaneous databases

    NextBioi 975482.

    Gene expression databases

    Genevestigatori P22133.

    Family and domain databases

    Gene3Di 3.40.50.720. 1 hit.
    3.90.110.10. 1 hit.
    InterProi IPR001557. L-lactate/malate_DH.
    IPR022383. Lactate/malate_DH_C.
    IPR001236. Lactate/malate_DH_N.
    IPR015955. Lactate_DH/Glyco_Ohase_4_C.
    IPR001252. Malate_DH_AS.
    IPR010097. Malate_DH_type1.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view ]
    PANTHERi PTHR11540. PTHR11540. 1 hit.
    Pfami PF02866. Ldh_1_C. 1 hit.
    PF00056. Ldh_1_N. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000102. Lac_mal_DH. 1 hit.
    SUPFAMi SSF56327. SSF56327. 1 hit.
    TIGRFAMsi TIGR01772. MDH_euk_gproteo. 1 hit.
    PROSITEi PS00068. MDH. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Isolation, nucleotide sequence analysis, and disruption of the MDH2 gene from Saccharomyces cerevisiae: evidence for three isozymes of yeast malate dehydrogenase."
      Minard K.I., McAlister-Henn L.
      Mol. Cell. Biol. 11:370-380(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-17.
    2. "Sequence analysis of a 13.4 kbp fragment from the left arm of chromosome XV reveals a malate dehydrogenase gene, a putative Ser/Thr protein kinase, the ribosomal L25 gene and four new open reading frames."
      Casamayor A., Khalid H., Balcells L., Aldea M., Casas C., Herrero E., Arino J.
      Yeast 12:1013-1020(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 96604 / S288c / FY1679.
    3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
      Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D.
      , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
      Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. "Purification procedure and N-terminal amino acid sequence of yeast malate dehydrogenase isoenzymes."
      Kopetzki E., Entian K.-D., Lottspeich F., Mecke D.
      Biochim. Biophys. Acta 912:398-403(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 2-35.
    6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    7. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-6, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiMDHC_YEAST
    AccessioniPrimary (citable) accession number: P22133
    Secondary accession number(s): D6W1U2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1991
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 138 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Yeast contains at least 3 malate dehydrogenase isoenzymes: a mitochondrial (MDH1), a cytoplasmic (MDH2) and a peroxisomal (MDH3).
    Present with 5260 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XV
      Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

    External Data

    Dasty 3