Reviewed, UniProtKB/Swiss-Prot P22133 (MDHC_YEAST)

Names and origin

Protein names

Recommended name:
    Malate dehydrogenase, cytoplasmic
    EC=1.1.1.37

Gene names

Name:	MDH2
Ordered Locus Names:	YOL126C

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	377 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	The isoenzyme MDH2 may function primarily in the glyoxylate cycle.
Catalytic activity	(S)-malate + NAD+ = oxaloacetate + NADH.
Subunit structure	Homodimer.
Subcellular location	Cytoplasm.
Induction	By acetate as carbon source in the growth medium. Is inactivated by addition of glucose (catabolite inactivation).
Miscellaneous	Yeast contains at least 3 malate dehydrogenase isoenzymes: a mitochondrial (MDH1), a cytoplasmic (MDH2) and a peroxisomal (MDH3). Present with 5260 molecules/cell in log phase SD medium. Ref.5
Sequence similarities	Belongs to the LDH/MDH superfamily. MDH type 1 family.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Oxidoreductase
PTM	Phosphoprotein
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	gluconeogenesis Inferred from mutant phenotype. Source: SGD glycolysis Inferred from electronic annotation. Source: InterPro malate metabolic process Traceable author statement. Source: SGD oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW protein import into peroxisome matrix Inferred from mutant phenotype. Source: SGD tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytosol Ref.1 Inferred from direct assay. Source: SGD
Molecular function	L-malate dehydrogenase activity Ref.1 Inferred from direct assay. Source: SGD protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
GET3	Q12154	1	EBI-10589,EBI-2989

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.1 Ref.4
	Chain	2 – 377	376	Malate dehydrogenase, cytoplasmic		PRO_0000113338
Regions
	Nucleotide binding	20 – 26	7	NAD By similarity
	Nucleotide binding	144 – 146	3	NAD By similarity
Sites
	Active site	215	1	Proton acceptor By similarity
	Binding site	57	1	NAD By similarity
	Binding site	106	1	Substrate By similarity
	Binding site	112	1	Substrate By similarity
	Binding site	119	1	NAD By similarity
	Binding site	146	1	Substrate By similarity
	Binding site	185	1	Substrate By similarity
	Binding site	266	1	NAD By similarity
Amino acid modifications
	Modified residue	6	1	Phosphothreonine Ref.6

Sequences

	Sequence		Length	Mass (Da)
	P22133-1 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: CAF4784FA7DD6E27	FASTA	377	40,731
	10 20 30 40 50 60 MPHSVTPSIE QDSLKIAILG AAGGIGQSLS LLLKAQLQYQ LKESNRSVTH IHLALYDVNQ 70 80 90 100 110 120 EAINGVTADL SHIDTPISVS SHSPAGGIEN CLHNASIVVI PAGVPRKPGM TRDDLFNVNA 130 140 150 160 170 180 GIISQLGDSI AECCDLSKVF VLVISNPVNS LVPVMVSNIL KNHPQSRNSG IERRIMGVTK 190 200 210 220 230 240 LDIVRASTFL REINIESGLT PRVNSMPDVP VIGGHSGETI IPLFSQSNFL SRLNEDQLKY 250 260 270 280 290 300 LIHRVQYGGD EVVKAKNGKG SATLSMAHAG YKCVVQFVSL LLGNIEQIHG TYYVPLKDAN 310 320 330 340 350 360 NFPIAPGADQ LLPLVDGADY FAIPLTITTK GVSYVDYDIV NRMNDMERNQ MLPICVSQLK 370 KNIDKGLEFV ASRSASS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation, nucleotide sequence analysis, and disruption of the MDH2 gene from Saccharomyces cerevisiae: evidence for three isozymes of yeast malate dehydrogenase." Minard K.I., McAlister-Henn L. Mol. Cell. Biol. 11:370-380(1991) [PubMed: 1986231] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-17.
[2]	"Sequence analysis of a 13.4 kbp fragment from the left arm of chromosome XV reveals a malate dehydrogenase gene, a putative Ser/Thr protein kinase, the ribosomal L25 gene and four new open reading frames." Casamayor A., Khalid H., Balcells L., Aldea M., Casas C., Herrero E., Arino J. Yeast 12:1013-1020(1996) [PubMed: 8896265] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XV." Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D. , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K. Nature 387:98-102(1997) [PubMed: 9169874] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[4]	"Purification procedure and N-terminal amino acid sequence of yeast malate dehydrogenase isoenzymes." Kopetzki E., Entian K.-D., Lottspeich F., Mecke D. Biochim. Biophys. Acta 912:398-403(1987) [PubMed: 3552052] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-35.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-6, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M62808 Genomic DNA. Translation: AAA34766.1. U41293 Genomic DNA. Translation: AAC49466.1. Different initiation. Z74868 Genomic DNA. Translation: CAA99145.1. Different initiation.
PIR	DEBYMC. S63444.
RefSeq	NP_014515.2.
3D structure databases
HSSP	HSSP built from PDB template 1MLD based on UniProtKB P00346.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:4211N.
IntAct	P22133. 8 interactions.
Proteomic databases
PeptideAtlas	P22133.
Genome annotation databases
Ensembl	YOL126C. Saccharomyces cerevisiae. [Contig view]
GeneID	853994.
GenomeReviews	Gene locus YOL126C in contig Y13140_GR.
KEGG	sce:YOL126C.
NMPDR	fig|4932.3.peg.5601.
Organism-specific databases
CYGD	YOL126c.
SGD	S000005486. MDH2.
Yeast-GFP	Search...
Phylogenomic databases
HOGENOM	P22133.
Enzyme and pathway databases
BRENDA	1.1.1.37. 250.
Gene expression databases
ArrayExpress	P22133.
GermOnline	YOL126C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR001557. L-lactate/malate_DH. IPR001236. Lactate/malate_DH. IPR015955. Lactate_DH/Glyco_Ohase_4_C. IPR001252. Malate_DH_AS. IPR010097. Malate_DH_NAD-dep_euk_g_bac. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.90.110.10. lact_mal_DH. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR11540:SF1. MDH_euk_g_bac. 1 hit.
Pfam	PF02866. Ldh_1_C. 1 hit. PF00056. Ldh_1_N. 1 hit. [Graphical view]
PIRSF	PIRSF000102. Lac_mal_DH. 1 hit.
TIGRFAMs	TIGR01772. MDH_euk_gproteo. 1 hit.
PROSITE	PS00068. MDH. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	975482.

Entry information

Entry name

MDHC_YEAST

Accession

Primary (citable) accession number: P22133

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1991
Last sequence update:	January 23, 2007
Last modified:	June 16, 2009
This is version 89 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)