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Protein

Malate dehydrogenase, cytoplasmic

Gene

MDH2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

The isoenzyme MDH2 may function primarily in the glyoxylate cycle.

Miscellaneous

Yeast contains at least 3 malate dehydrogenase isoenzymes: a mitochondrial (MDH1), a cytoplasmic (MDH2) and a peroxisomal (MDH3).
Present with 5260 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

(S)-malate + NAD+ = oxaloacetate + NADH.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei57NADBy similarity1
Binding sitei106SubstratePROSITE-ProRule annotation1
Binding sitei112SubstratePROSITE-ProRule annotation1
Binding sitei119NADBy similarity1
Binding sitei146SubstratePROSITE-ProRule annotation1
Binding sitei185SubstratePROSITE-ProRule annotation1
Active sitei215Proton acceptorBy similarity1
Binding sitei266NADBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi20 – 26NADBy similarity7
Nucleotide bindingi144 – 146NADBy similarity3

GO - Molecular functioni

  • L-malate dehydrogenase activity Source: SGD

GO - Biological processi

  • gluconeogenesis Source: SGD
  • malate metabolic process Source: SGD
  • protein import into peroxisome matrix Source: SGD
  • tricarboxylic acid cycle Source: UniProtKB-KW

Keywordsi

Molecular functionOxidoreductase
Biological processTricarboxylic acid cycle
LigandNAD

Enzyme and pathway databases

BioCyciYEAST:YOL126C-MONOMER
BRENDAi1.1.1.37 984
SABIO-RKiP22133

Names & Taxonomyi

Protein namesi
Recommended name:
Malate dehydrogenase, cytoplasmic (EC:1.1.1.37)
Gene namesi
Name:MDH2
Ordered Locus Names:YOL126C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOL126C
SGDiS000005486 MDH2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved2 Publications
ChainiPRO_00001133382 – 377Malate dehydrogenase, cytoplasmicAdd BLAST376

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei6PhosphothreonineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP22133
PaxDbiP22133
PRIDEiP22133

PTM databases

iPTMnetiP22133

Expressioni

Inductioni

By acetate as carbon source in the growth medium. Is inactivated by addition of glucose (catabolite inactivation).

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

BioGridi34249, 166 interactors
DIPiDIP-4211N
IntActiP22133, 33 interactors
MINTiP22133
STRINGi4932.YOL126C

Structurei

3D structure databases

ProteinModelPortaliP22133
SMRiP22133
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. MDH type 1 family.Curated

Phylogenomic databases

GeneTreeiENSGT00550000075847
HOGENOMiHOG000213792
InParanoidiP22133
KOiK00026
OMAiEIYQLGP
OrthoDBiEOG092C2KVE

Family and domain databases

Gene3Di3.90.110.10, 1 hit
InterProiView protein in InterPro
IPR001557 L-lactate/malate_DH
IPR022383 Lactate/malate_DH_C
IPR001236 Lactate/malate_DH_N
IPR015955 Lactate_DH/Glyco_Ohase_4_C
IPR001252 Malate_DH_AS
IPR010097 Malate_DH_type1
IPR036291 NAD(P)-bd_dom_sf
PfamiView protein in Pfam
PF02866 Ldh_1_C, 1 hit
PF00056 Ldh_1_N, 1 hit
PIRSFiPIRSF000102 Lac_mal_DH, 1 hit
SUPFAMiSSF51735 SSF51735, 1 hit
SSF56327 SSF56327, 1 hit
TIGRFAMsiTIGR01772 MDH_euk_gproteo, 1 hit
PROSITEiView protein in PROSITE
PS00068 MDH, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P22133-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPHSVTPSIE QDSLKIAILG AAGGIGQSLS LLLKAQLQYQ LKESNRSVTH
60 70 80 90 100
IHLALYDVNQ EAINGVTADL SHIDTPISVS SHSPAGGIEN CLHNASIVVI
110 120 130 140 150
PAGVPRKPGM TRDDLFNVNA GIISQLGDSI AECCDLSKVF VLVISNPVNS
160 170 180 190 200
LVPVMVSNIL KNHPQSRNSG IERRIMGVTK LDIVRASTFL REINIESGLT
210 220 230 240 250
PRVNSMPDVP VIGGHSGETI IPLFSQSNFL SRLNEDQLKY LIHRVQYGGD
260 270 280 290 300
EVVKAKNGKG SATLSMAHAG YKCVVQFVSL LLGNIEQIHG TYYVPLKDAN
310 320 330 340 350
NFPIAPGADQ LLPLVDGADY FAIPLTITTK GVSYVDYDIV NRMNDMERNQ
360 370
MLPICVSQLK KNIDKGLEFV ASRSASS
Length:377
Mass (Da):40,731
Last modified:January 23, 2007 - v2
Checksum:iCAF4784FA7DD6E27
GO

Sequence cautioni

The sequence AAC49466 differs from that shown. Reason: Erroneous initiation.Curated
The sequence CAA99145 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M62808 Genomic DNA Translation: AAA34766.1
U41293 Genomic DNA Translation: AAC49466.1 Different initiation.
Z74868 Genomic DNA Translation: CAA99145.1 Different initiation.
BK006948 Genomic DNA Translation: DAA10658.1
PIRiS63444 DEBYMC
RefSeqiNP_014515.2, NM_001183380.1

Genome annotation databases

EnsemblFungiiYOL126C; YOL126C; YOL126C
GeneIDi853994
KEGGisce:YOL126C

Similar proteinsi

Entry informationi

Entry nameiMDHC_YEAST
AccessioniPrimary (citable) accession number: P22133
Secondary accession number(s): D6W1U2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: January 23, 2007
Last modified: May 23, 2018
This is version 169 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

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