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Protein

Malate dehydrogenase, cytoplasmic

Gene

MDH2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The isoenzyme MDH2 may function primarily in the glyoxylate cycle.

Catalytic activityi

(S)-malate + NAD+ = oxaloacetate + NADH.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei57NADBy similarity1
Binding sitei106SubstratePROSITE-ProRule annotation1
Binding sitei112SubstratePROSITE-ProRule annotation1
Binding sitei119NADBy similarity1
Binding sitei146SubstratePROSITE-ProRule annotation1
Binding sitei185SubstratePROSITE-ProRule annotation1
Active sitei215Proton acceptorBy similarity1
Binding sitei266NADBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi20 – 26NADBy similarity7
Nucleotide bindingi144 – 146NADBy similarity3

GO - Molecular functioni

  • L-malate dehydrogenase activity Source: SGD

GO - Biological processi

  • gluconeogenesis Source: SGD
  • malate metabolic process Source: SGD
  • protein import into peroxisome matrix Source: SGD
  • tricarboxylic acid cycle Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Tricarboxylic acid cycle

Keywords - Ligandi

NAD

Enzyme and pathway databases

BioCyciYEAST:YOL126C-MONOMER.
BRENDAi1.1.1.37. 984.
SABIO-RKP22133.

Names & Taxonomyi

Protein namesi
Recommended name:
Malate dehydrogenase, cytoplasmic (EC:1.1.1.37)
Gene namesi
Name:MDH2
Ordered Locus Names:YOL126C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOL126C.
SGDiS000005486. MDH2.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: SGD
  • nuclear periphery Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved2 Publications
ChainiPRO_00001133382 – 377Malate dehydrogenase, cytoplasmicAdd BLAST376

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei6PhosphothreonineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP22133.
PRIDEiP22133.

PTM databases

iPTMnetiP22133.

Expressioni

Inductioni

By acetate as carbon source in the growth medium. Is inactivated by addition of glucose (catabolite inactivation).

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

BioGridi34249. 39 interactors.
DIPiDIP-4211N.
IntActiP22133. 6 interactors.
MINTiMINT-568274.

Structurei

3D structure databases

ProteinModelPortaliP22133.
SMRiP22133.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. MDH type 1 family.Curated

Phylogenomic databases

GeneTreeiENSGT00550000075847.
HOGENOMiHOG000213792.
InParanoidiP22133.
KOiK00026.
OMAiNDMERNQ.
OrthoDBiEOG092C2KVE.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
InterProiIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR001252. Malate_DH_AS.
IPR010097. Malate_DH_type1.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11540. PTHR11540. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01772. MDH_euk_gproteo. 1 hit.
PROSITEiPS00068. MDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P22133-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPHSVTPSIE QDSLKIAILG AAGGIGQSLS LLLKAQLQYQ LKESNRSVTH
60 70 80 90 100
IHLALYDVNQ EAINGVTADL SHIDTPISVS SHSPAGGIEN CLHNASIVVI
110 120 130 140 150
PAGVPRKPGM TRDDLFNVNA GIISQLGDSI AECCDLSKVF VLVISNPVNS
160 170 180 190 200
LVPVMVSNIL KNHPQSRNSG IERRIMGVTK LDIVRASTFL REINIESGLT
210 220 230 240 250
PRVNSMPDVP VIGGHSGETI IPLFSQSNFL SRLNEDQLKY LIHRVQYGGD
260 270 280 290 300
EVVKAKNGKG SATLSMAHAG YKCVVQFVSL LLGNIEQIHG TYYVPLKDAN
310 320 330 340 350
NFPIAPGADQ LLPLVDGADY FAIPLTITTK GVSYVDYDIV NRMNDMERNQ
360 370
MLPICVSQLK KNIDKGLEFV ASRSASS
Length:377
Mass (Da):40,731
Last modified:January 23, 2007 - v2
Checksum:iCAF4784FA7DD6E27
GO

Sequence cautioni

The sequence AAC49466 differs from that shown. Reason: Erroneous initiation.Curated
The sequence CAA99145 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M62808 Genomic DNA. Translation: AAA34766.1.
U41293 Genomic DNA. Translation: AAC49466.1. Different initiation.
Z74868 Genomic DNA. Translation: CAA99145.1. Different initiation.
BK006948 Genomic DNA. Translation: DAA10658.1.
PIRiS63444. DEBYMC.
RefSeqiNP_014515.2. NM_001183380.1.

Genome annotation databases

EnsemblFungiiYOL126C; YOL126C; YOL126C.
GeneIDi853994.
KEGGisce:YOL126C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M62808 Genomic DNA. Translation: AAA34766.1.
U41293 Genomic DNA. Translation: AAC49466.1. Different initiation.
Z74868 Genomic DNA. Translation: CAA99145.1. Different initiation.
BK006948 Genomic DNA. Translation: DAA10658.1.
PIRiS63444. DEBYMC.
RefSeqiNP_014515.2. NM_001183380.1.

3D structure databases

ProteinModelPortaliP22133.
SMRiP22133.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34249. 39 interactors.
DIPiDIP-4211N.
IntActiP22133. 6 interactors.
MINTiMINT-568274.

PTM databases

iPTMnetiP22133.

Proteomic databases

MaxQBiP22133.
PRIDEiP22133.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYOL126C; YOL126C; YOL126C.
GeneIDi853994.
KEGGisce:YOL126C.

Organism-specific databases

EuPathDBiFungiDB:YOL126C.
SGDiS000005486. MDH2.

Phylogenomic databases

GeneTreeiENSGT00550000075847.
HOGENOMiHOG000213792.
InParanoidiP22133.
KOiK00026.
OMAiNDMERNQ.
OrthoDBiEOG092C2KVE.

Enzyme and pathway databases

BioCyciYEAST:YOL126C-MONOMER.
BRENDAi1.1.1.37. 984.
SABIO-RKP22133.

Miscellaneous databases

PROiP22133.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
InterProiIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR001252. Malate_DH_AS.
IPR010097. Malate_DH_type1.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11540. PTHR11540. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01772. MDH_euk_gproteo. 1 hit.
PROSITEiPS00068. MDH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMDHC_YEAST
AccessioniPrimary (citable) accession number: P22133
Secondary accession number(s): D6W1U2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 157 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Yeast contains at least 3 malate dehydrogenase isoenzymes: a mitochondrial (MDH1), a cytoplasmic (MDH2) and a peroxisomal (MDH3).
Present with 5260 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.