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Protein

Phosphoenolpyruvate carboxykinase [GTP]

Gene
N/A
Organism
Neocallimastix frontalis (Rumen fungus)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.By similarity

Catalytic activityi

GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2.

Cofactori

Mn2+By similarityNote: Binds 1 Mn2+ ion per subunit.By similarity

Pathwayi: gluconeogenesis

This protein is involved in the pathway gluconeogenesis, which is part of Carbohydrate biosynthesis.
View all proteins of this organism that are known to be involved in the pathway gluconeogenesis and in Carbohydrate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei75SubstrateBy similarity1
Metal bindingi224ManganeseBy similarity1
Metal bindingi245Manganese; via tele nitrogenBy similarity1
Binding sitei267SubstrateBy similarity1
Active sitei269By similarity1
Metal bindingi292ManganeseBy similarity1
Binding sitei388GTPBy similarity1
Binding sitei419GTPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi268 – 273GTPBy similarity6
Nucleotide bindingi513 – 516GTPBy similarity4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDecarboxylase, Lyase
Biological processGluconeogenesis
LigandGTP-binding, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00138.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoenolpyruvate carboxykinase [GTP] (EC:4.1.1.32)
Short name:
PEPCK
OrganismiNeocallimastix frontalis (Rumen fungus)
Taxonomic identifieri4757 [NCBI]
Taxonomic lineageiEukaryotaFungiNeocallimastigomycotaNeocallimastigomycetesNeocallimastigalesNeocallimastigaceaeNeocallimastix

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001036361 – 608Phosphoenolpyruvate carboxykinase [GTP]Add BLAST608

Interactioni

Subunit structurei

Monomer.By similarity

Structurei

3D structure databases

ProteinModelPortaliP22130.
SMRiP22130.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni215 – 217Substrate bindingBy similarity3
Regioni386 – 388Substrate bindingBy similarity3

Sequence similaritiesi

Family and domain databases

CDDicd00819. PEPCK_GTP. 1 hit.
Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00452. PEPCK_GTP. 1 hit.
InterProiView protein in InterPro
IPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR035077. PEP_carboxykinase_GTP_C.
IPR035078. PEP_carboxykinase_GTP_N.
IPR008210. PEP_carboxykinase_N.
PANTHERiPTHR11561. PTHR11561. 1 hit.
PfamiView protein in Pfam
PF00821. PEPCK_C. 1 hit.
PF17297. PEPCK_N. 1 hit.
PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
PROSITEiView protein in PROSITE
PS00505. PEPCK_GTP. 1 hit.

Sequencei

Sequence statusi: Complete.

P22130-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAHSVSSVVN KQLLAYIKES SELMTPKDIY VCDGSAEEYH NLCELLVKQG
60 70 80 90 100
IFTKLNETKR PNCYLARSNP ADVARVEKCT YICSEKEEDA GPTNNWMAPA
110 120 130 140 150
EMKAKLNGLM KGCMKGRTMY VIPFSMGPIG GPISRVGVEI TDSPYVVVNM
160 170 180 190 200
CIMAKVGKKV LDLLGVDGTF VPCTHSCLAP LEEGQKDSTW PCNIDNRYIV
210 220 230 240 250
QFPEEHRIVS VGSGYGGNAL LGKKCYALRI ATVLSREAGD SLAEHMLILG
260 270 280 290 300
ITNPQGKKYY ITAAFPSACG KTNLAMLNAT IPGWKIECVG DDIAWLKIGK
310 320 330 340 350
DGRLWAINPE SGFFGVAPGT SYKSNPNAMK SCEKDTIFTN VALTEDGDVW
360 370 380 390 400
WEGMTKEVPK GKIITWLGKE WSADSGEPKP NLAHPNSRFT ARVENVPVGD
410 420 430 440 450
PGYYALEGVP VSAMIFGGRR ENTVPLVFQS RSWKHGVLLG SSVASETTAA
460 470 480 490 500
AEAAAGQLRF DPFAMLPFCG YNMGDYFGYW LSFADKYDEA KLPKIFHVNW
510 520 530 540 550
FRKDNGRFLW PGYGENSRVL KWIIERVEGK EGIAKETPIG YLPAKGALDL
560 570 580 590 600
SGLDVPEADM EKILTVDCKA YLSEVEKIRQ YHSKFGSLLP KALIAELDAL

EQRLKAAL
Length:608
Mass (Da):66,904
Last modified:August 1, 1991 - v1
Checksum:iC4D2B249A92B7D26
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M59372 mRNA. Translation: AAA33553.1.
PIRiJQ1462.

Similar proteinsi

Entry informationi

Entry nameiPCKG_NEOFR
AccessioniPrimary (citable) accession number: P22130
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: August 1, 1991
Last modified: September 27, 2017
This is version 83 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families