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P22130

- PCKG_NEOFR

UniProt

P22130 - PCKG_NEOFR

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Protein

Phosphoenolpyruvate carboxykinase [GTP]

Gene
N/A
Organism
Neocallimastix frontalis (Rumen fungus)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli

Functioni

Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.By similarity

Catalytic activityi

GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2.

Cofactori

Mn2+By similarityNote: Binds 1 Mn(2+) ion per subunit.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei75 – 751SubstrateBy similarity
Binding sitei217 – 2171Substrate; via amide nitrogenBy similarity
Metal bindingi224 – 2241ManganeseBy similarity
Binding sitei224 – 2241SubstrateBy similarity
Metal bindingi245 – 2451Manganese; via tele nitrogenBy similarity
Binding sitei267 – 2671SubstrateBy similarity
Active sitei269 – 2691Sequence Analysis
Metal bindingi292 – 2921ManganeseBy similarity
Binding sitei388 – 3881GTPBy similarity
Binding sitei419 – 4191GTPBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi268 – 2736GTPBy similarity
Nucleotide bindingi513 – 5164GTPBy similarity

GO - Molecular functioni

  1. GTP binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-KW
  3. phosphoenolpyruvate carboxykinase (GTP) activity Source: UniProtKB-EC

GO - Biological processi

  1. gluconeogenesis Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Decarboxylase, Lyase

Keywords - Biological processi

Gluconeogenesis

Keywords - Ligandi

GTP-binding, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00138.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoenolpyruvate carboxykinase [GTP] (EC:4.1.1.32)
Short name:
PEPCK
OrganismiNeocallimastix frontalis (Rumen fungus)
Taxonomic identifieri4757 [NCBI]
Taxonomic lineageiEukaryotaFungiNeocallimastigomycotaNeocallimastigomycetesNeocallimastigalesNeocallimastigaceaeNeocallimastix

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 608608Phosphoenolpyruvate carboxykinase [GTP]PRO_0000103636Add
BLAST

Interactioni

Subunit structurei

Monomer.By similarity

Structurei

3D structure databases

ProteinModelPortaliP22130.
SMRiP22130. Positions 8-605.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni386 – 3883Substrate bindingBy similarity

Sequence similaritiesi

Family and domain databases

Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00452. PEPCK_GTP.
InterProiIPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PANTHERiPTHR11561. PTHR11561. 1 hit.
PfamiPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
PROSITEiPS00505. PEPCK_GTP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P22130-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAHSVSSVVN KQLLAYIKES SELMTPKDIY VCDGSAEEYH NLCELLVKQG
60 70 80 90 100
IFTKLNETKR PNCYLARSNP ADVARVEKCT YICSEKEEDA GPTNNWMAPA
110 120 130 140 150
EMKAKLNGLM KGCMKGRTMY VIPFSMGPIG GPISRVGVEI TDSPYVVVNM
160 170 180 190 200
CIMAKVGKKV LDLLGVDGTF VPCTHSCLAP LEEGQKDSTW PCNIDNRYIV
210 220 230 240 250
QFPEEHRIVS VGSGYGGNAL LGKKCYALRI ATVLSREAGD SLAEHMLILG
260 270 280 290 300
ITNPQGKKYY ITAAFPSACG KTNLAMLNAT IPGWKIECVG DDIAWLKIGK
310 320 330 340 350
DGRLWAINPE SGFFGVAPGT SYKSNPNAMK SCEKDTIFTN VALTEDGDVW
360 370 380 390 400
WEGMTKEVPK GKIITWLGKE WSADSGEPKP NLAHPNSRFT ARVENVPVGD
410 420 430 440 450
PGYYALEGVP VSAMIFGGRR ENTVPLVFQS RSWKHGVLLG SSVASETTAA
460 470 480 490 500
AEAAAGQLRF DPFAMLPFCG YNMGDYFGYW LSFADKYDEA KLPKIFHVNW
510 520 530 540 550
FRKDNGRFLW PGYGENSRVL KWIIERVEGK EGIAKETPIG YLPAKGALDL
560 570 580 590 600
SGLDVPEADM EKILTVDCKA YLSEVEKIRQ YHSKFGSLLP KALIAELDAL

EQRLKAAL
Length:608
Mass (Da):66,904
Last modified:August 1, 1991 - v1
Checksum:iC4D2B249A92B7D26
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M59372 mRNA. Translation: AAA33553.1.
PIRiJQ1462.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M59372 mRNA. Translation: AAA33553.1 .
PIRi JQ1462.

3D structure databases

ProteinModelPortali P22130.
SMRi P22130. Positions 8-605.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00138 .

Family and domain databases

Gene3Di 3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPi MF_00452. PEPCK_GTP.
InterProi IPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view ]
PANTHERi PTHR11561. PTHR11561. 1 hit.
Pfami PF00821. PEPCK. 1 hit.
[Graphical view ]
PIRSFi PIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMi SSF68923. SSF68923. 1 hit.
PROSITEi PS00505. PEPCK_GTP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Sequence of the phosphoenolpyruvate carboxykinase-encoding cDNA from the rumen anaerobic fungus Neocallimastix frontalis: comparison of the amino acid sequence with animals and yeast."
    Reymond P., Geourjon C., Roux B., Durand R., Fevre M.
    Gene 110:57-63(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Entry informationi

Entry nameiPCKG_NEOFR
AccessioniPrimary (citable) accession number: P22130
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: August 1, 1991
Last modified: November 26, 2014
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3