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P22130

- PCKG_NEOFR

UniProt

P22130 - PCKG_NEOFR

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Protein

Phosphoenolpyruvate carboxykinase [GTP]

Gene
N/A
Organism
Neocallimastix frontalis (Rumen fungus)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at transcript leveli

Functioni

Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle By similarity.UniRule annotation

Catalytic activityi

GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2.UniRule annotation

Cofactori

Binds 1 manganese ion per subunit By similarity.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei75 – 751Substrate By similarity
Binding sitei217 – 2171Substrate; via amide nitrogen By similarity
Metal bindingi224 – 2241Manganese By similarity
Binding sitei224 – 2241Substrate By similarity
Metal bindingi245 – 2451Manganese; via tele nitrogen By similarity
Binding sitei267 – 2671Substrate By similarity
Active sitei269 – 2691 Reviewed prediction
Metal bindingi292 – 2921Manganese By similarity
Binding sitei388 – 3881GTP By similarity
Binding sitei419 – 4191GTP By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi268 – 2736GTP By similarity
Nucleotide bindingi513 – 5164GTP By similarity

GO - Molecular functioni

  1. GTP binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-KW
  3. phosphoenolpyruvate carboxykinase (GTP) activity Source: UniProtKB-EC

GO - Biological processi

  1. gluconeogenesis Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Decarboxylase, Lyase

Keywords - Biological processi

Gluconeogenesis

Keywords - Ligandi

GTP-binding, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00138.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoenolpyruvate carboxykinase [GTP] (EC:4.1.1.32)
Short name:
PEPCK
OrganismiNeocallimastix frontalis (Rumen fungus)
Taxonomic identifieri4757 [NCBI]
Taxonomic lineageiEukaryotaFungiNeocallimastigomycotaNeocallimastigomycetesNeocallimastigalesNeocallimastigaceaeNeocallimastix

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 608608Phosphoenolpyruvate carboxykinase [GTP]UniRule annotationPRO_0000103636Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliP22130.
SMRiP22130. Positions 8-605.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni386 – 3883Substrate binding By similarity

Sequence similaritiesi

Family and domain databases

Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00452. PEPCK_GTP.
InterProiIPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PANTHERiPTHR11561. PTHR11561. 1 hit.
PfamiPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
PROSITEiPS00505. PEPCK_GTP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P22130-1 [UniParc]FASTAAdd to Basket

« Hide

MAHSVSSVVN KQLLAYIKES SELMTPKDIY VCDGSAEEYH NLCELLVKQG    50
IFTKLNETKR PNCYLARSNP ADVARVEKCT YICSEKEEDA GPTNNWMAPA 100
EMKAKLNGLM KGCMKGRTMY VIPFSMGPIG GPISRVGVEI TDSPYVVVNM 150
CIMAKVGKKV LDLLGVDGTF VPCTHSCLAP LEEGQKDSTW PCNIDNRYIV 200
QFPEEHRIVS VGSGYGGNAL LGKKCYALRI ATVLSREAGD SLAEHMLILG 250
ITNPQGKKYY ITAAFPSACG KTNLAMLNAT IPGWKIECVG DDIAWLKIGK 300
DGRLWAINPE SGFFGVAPGT SYKSNPNAMK SCEKDTIFTN VALTEDGDVW 350
WEGMTKEVPK GKIITWLGKE WSADSGEPKP NLAHPNSRFT ARVENVPVGD 400
PGYYALEGVP VSAMIFGGRR ENTVPLVFQS RSWKHGVLLG SSVASETTAA 450
AEAAAGQLRF DPFAMLPFCG YNMGDYFGYW LSFADKYDEA KLPKIFHVNW 500
FRKDNGRFLW PGYGENSRVL KWIIERVEGK EGIAKETPIG YLPAKGALDL 550
SGLDVPEADM EKILTVDCKA YLSEVEKIRQ YHSKFGSLLP KALIAELDAL 600
EQRLKAAL 608
Length:608
Mass (Da):66,904
Last modified:August 1, 1991 - v1
Checksum:iC4D2B249A92B7D26
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M59372 mRNA. Translation: AAA33553.1.
PIRiJQ1462.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M59372 mRNA. Translation: AAA33553.1 .
PIRi JQ1462.

3D structure databases

ProteinModelPortali P22130.
SMRi P22130. Positions 8-605.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00138 .

Family and domain databases

Gene3Di 3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPi MF_00452. PEPCK_GTP.
InterProi IPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view ]
PANTHERi PTHR11561. PTHR11561. 1 hit.
Pfami PF00821. PEPCK. 1 hit.
[Graphical view ]
PIRSFi PIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMi SSF68923. SSF68923. 1 hit.
PROSITEi PS00505. PEPCK_GTP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Sequence of the phosphoenolpyruvate carboxykinase-encoding cDNA from the rumen anaerobic fungus Neocallimastix frontalis: comparison of the amino acid sequence with animals and yeast."
    Reymond P., Geourjon C., Roux B., Durand R., Fevre M.
    Gene 110:57-63(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Entry informationi

Entry nameiPCKG_NEOFR
AccessioniPrimary (citable) accession number: P22130
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: August 1, 1991
Last modified: April 16, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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