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P22130

- PCKG_NEOFR

UniProt

P22130 - PCKG_NEOFR

Protein

Phosphoenolpyruvate carboxykinase [GTP]

Gene
N/A
Organism
Neocallimastix frontalis (Rumen fungus)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 74 (01 Oct 2014)
      Sequence version 1 (01 Aug 1991)
      Previous versions | rss
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    Functioni

    Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.By similarity

    Catalytic activityi

    GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2.

    Cofactori

    Binds 1 manganese ion per subunit.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei75 – 751SubstrateBy similarity
    Binding sitei217 – 2171Substrate; via amide nitrogenBy similarity
    Metal bindingi224 – 2241ManganeseBy similarity
    Binding sitei224 – 2241SubstrateBy similarity
    Metal bindingi245 – 2451Manganese; via tele nitrogenBy similarity
    Binding sitei267 – 2671SubstrateBy similarity
    Active sitei269 – 2691Sequence Analysis
    Metal bindingi292 – 2921ManganeseBy similarity
    Binding sitei388 – 3881GTPBy similarity
    Binding sitei419 – 4191GTPBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi268 – 2736GTPBy similarity
    Nucleotide bindingi513 – 5164GTPBy similarity

    GO - Molecular functioni

    1. GTP binding Source: UniProtKB-KW
    2. metal ion binding Source: UniProtKB-KW
    3. phosphoenolpyruvate carboxykinase (GTP) activity Source: UniProtKB-EC

    GO - Biological processi

    1. gluconeogenesis Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Decarboxylase, Lyase

    Keywords - Biological processi

    Gluconeogenesis

    Keywords - Ligandi

    GTP-binding, Manganese, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    UniPathwayiUPA00138.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phosphoenolpyruvate carboxykinase [GTP] (EC:4.1.1.32)
    Short name:
    PEPCK
    OrganismiNeocallimastix frontalis (Rumen fungus)
    Taxonomic identifieri4757 [NCBI]
    Taxonomic lineageiEukaryotaFungiNeocallimastigomycotaNeocallimastigomycetesNeocallimastigalesNeocallimastigaceaeNeocallimastix

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 608608Phosphoenolpyruvate carboxykinase [GTP]PRO_0000103636Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliP22130.
    SMRiP22130. Positions 8-605.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni386 – 3883Substrate bindingBy similarity

    Sequence similaritiesi

    Family and domain databases

    Gene3Di3.40.449.10. 1 hit.
    3.90.228.20. 2 hits.
    HAMAPiMF_00452. PEPCK_GTP.
    InterProiIPR018091. PEP_carboxykin_GTP_CS.
    IPR013035. PEP_carboxykinase_C.
    IPR008209. PEP_carboxykinase_GTP.
    IPR008210. PEP_carboxykinase_N.
    [Graphical view]
    PANTHERiPTHR11561. PTHR11561. 1 hit.
    PfamiPF00821. PEPCK. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
    SUPFAMiSSF68923. SSF68923. 1 hit.
    PROSITEiPS00505. PEPCK_GTP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P22130-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAHSVSSVVN KQLLAYIKES SELMTPKDIY VCDGSAEEYH NLCELLVKQG    50
    IFTKLNETKR PNCYLARSNP ADVARVEKCT YICSEKEEDA GPTNNWMAPA 100
    EMKAKLNGLM KGCMKGRTMY VIPFSMGPIG GPISRVGVEI TDSPYVVVNM 150
    CIMAKVGKKV LDLLGVDGTF VPCTHSCLAP LEEGQKDSTW PCNIDNRYIV 200
    QFPEEHRIVS VGSGYGGNAL LGKKCYALRI ATVLSREAGD SLAEHMLILG 250
    ITNPQGKKYY ITAAFPSACG KTNLAMLNAT IPGWKIECVG DDIAWLKIGK 300
    DGRLWAINPE SGFFGVAPGT SYKSNPNAMK SCEKDTIFTN VALTEDGDVW 350
    WEGMTKEVPK GKIITWLGKE WSADSGEPKP NLAHPNSRFT ARVENVPVGD 400
    PGYYALEGVP VSAMIFGGRR ENTVPLVFQS RSWKHGVLLG SSVASETTAA 450
    AEAAAGQLRF DPFAMLPFCG YNMGDYFGYW LSFADKYDEA KLPKIFHVNW 500
    FRKDNGRFLW PGYGENSRVL KWIIERVEGK EGIAKETPIG YLPAKGALDL 550
    SGLDVPEADM EKILTVDCKA YLSEVEKIRQ YHSKFGSLLP KALIAELDAL 600
    EQRLKAAL 608
    Length:608
    Mass (Da):66,904
    Last modified:August 1, 1991 - v1
    Checksum:iC4D2B249A92B7D26
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M59372 mRNA. Translation: AAA33553.1.
    PIRiJQ1462.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M59372 mRNA. Translation: AAA33553.1 .
    PIRi JQ1462.

    3D structure databases

    ProteinModelPortali P22130.
    SMRi P22130. Positions 8-605.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    UniPathwayi UPA00138 .

    Family and domain databases

    Gene3Di 3.40.449.10. 1 hit.
    3.90.228.20. 2 hits.
    HAMAPi MF_00452. PEPCK_GTP.
    InterProi IPR018091. PEP_carboxykin_GTP_CS.
    IPR013035. PEP_carboxykinase_C.
    IPR008209. PEP_carboxykinase_GTP.
    IPR008210. PEP_carboxykinase_N.
    [Graphical view ]
    PANTHERi PTHR11561. PTHR11561. 1 hit.
    Pfami PF00821. PEPCK. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001348. PEP_carboxykinase_GTP. 1 hit.
    SUPFAMi SSF68923. SSF68923. 1 hit.
    PROSITEi PS00505. PEPCK_GTP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence of the phosphoenolpyruvate carboxykinase-encoding cDNA from the rumen anaerobic fungus Neocallimastix frontalis: comparison of the amino acid sequence with animals and yeast."
      Reymond P., Geourjon C., Roux B., Durand R., Fevre M.
      Gene 110:57-63(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].

    Entry informationi

    Entry nameiPCKG_NEOFR
    AccessioniPrimary (citable) accession number: P22130
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1991
    Last sequence update: August 1, 1991
    Last modified: October 1, 2014
    This is version 74 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3