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Protein

Heat shock factor protein

Gene

HSF

Organism
Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. Also required for growth at normal temperatures.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi194 – 299By similarityAdd BLAST106

GO - Molecular functioni

  • DNA binding Source: CAFA
  • sequence-specific DNA binding Source: InterPro
  • transcription factor activity, sequence-specific DNA binding Source: InterPro

GO - Biological processi

Keywordsi

Molecular functionActivator, DNA-binding
Biological processStress response, Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Heat shock factor protein
Short name:
HSF
Alternative name(s):
Heat shock transcription factor
Short name:
HSTF
Gene namesi
Name:HSF
Ordered Locus Names:KLLA0D03322g
OrganismiKluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica)
Taxonomic identifieri284590 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeKluyveromyces
Proteomesi
  • UP000000598 Componenti: Chromosome D

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: UniProtKB-SubCell
  • protein-DNA complex Source: CAFA

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001245791 – 677Heat shock factor proteinAdd BLAST677

Post-translational modificationi

Exhibits temperature-dependent phosphorylation.

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiP22121.

Interactioni

Subunit structurei

Homotrimer.

Protein-protein interaction databases

STRINGi284590.XP_453214.1.

Structurei

Secondary structure

1677
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi196 – 206Combined sources11
Helixi208 – 210Combined sources3
Turni211 – 213Combined sources3
Beta strandi214 – 216Combined sources3
Beta strandi218 – 221Combined sources4
Beta strandi222 – 226Combined sources5
Helixi228 – 234Combined sources7
Helixi236 – 239Combined sources4
Beta strandi240 – 242Combined sources3
Helixi245 – 254Combined sources10
Beta strandi257 – 259Combined sources3
Turni266 – 269Combined sources4
Beta strandi276 – 280Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1FBQX-ray2.00A/B195-281[»]
1FBSX-ray2.00A/B195-281[»]
1FBUX-ray2.00A/B195-281[»]
1FYKX-ray2.50A193-284[»]
1FYLX-ray2.10A/B193-284[»]
1FYMX-ray2.20A/B193-284[»]
2HTSX-ray1.83A193-281[»]
3HSFNMR-A193-281[»]
3HTSX-ray1.75B193-281[»]
DisProtiDP00036.
ProteinModelPortaliP22121.
SMRiP22121.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP22121.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni320 – 372Involved in trimerizationBy similarityAdd BLAST53
Regioni466 – 677ActivatoryAdd BLAST212

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi458 – 465Poly-Ser8
Compositional biasi485 – 498Poly-GlnAdd BLAST14

Sequence similaritiesi

Belongs to the HSF family.Curated

Phylogenomic databases

eggNOGiKOG0627. Eukaryota.
COG5169. LUCA.
InParanoidiP22121.
KOiK09419.
OrthoDBiEOG092C2G9O.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiView protein in InterPro
IPR000232. HSF_DNA-bd.
IPR027725. HSF_fam.
IPR011991. WHTH_DNA-bd_dom.
PANTHERiPTHR10015. PTHR10015. 1 hit.
PfamiView protein in Pfam
PF00447. HSF_DNA-bind. 1 hit.
PRINTSiPR00056. HSFDOMAIN.
SMARTiView protein in SMART
SM00415. HSF. 1 hit.
SUPFAMiSSF46785. SSF46785. 1 hit.
PROSITEiView protein in PROSITE
PS00434. HSF_DOMAIN. 1 hit.

Sequencei

Sequence statusi: Complete.

P22121-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGHNDSVETM DEISNPNNIL LPHDGTGLDA TGISGSQEPY GMVDVLNPDS
60 70 80 90 100
LKDDSNVDEP LIEDIVNPSL DPEGVVSAEP SNEVGTPLLQ QPISLDHVIT
110 120 130 140 150
RPASAGGVYS IGNSSTSSAA KLSDGDLTNA TDPLLNNAHG HGQPSSESQS
160 170 180 190 200
HSNGYHKQGQ SQQPLLSLNK RKLLAKAHVD KHHSKKKLST TRARPAFVNK
210 220 230 240 250
LWSMVNDKSN EKFIHWSTSG ESIVVPNRER FVQEVLPKYF KHSNFASFVR
260 270 280 290 300
QLNMYGWHKV QDVKSGSMLS NNDSRWEFEN ENFKRGKEYL LENIVRQKSN
310 320 330 340 350
TNILGGTTNA EVDIHILLNE LETVKYNQLA IAEDLKRITK DNEMLWKENM
360 370 380 390 400
MARERHQSQQ QVLEKLLRFL SSVFGPNSAK TIGNGFQPDL IHELSDMQVN
410 420 430 440 450
HMSNNNHNNT GNINPNAYHN ETDDPMANVF GPLTPTDQGK VPLQDYKLRP
460 470 480 490 500
RLLLKNRSMS SSSSSNLNQR QSPQNRIVGQ SPPPQQQQQQ QQQQGQPQGQ
510 520 530 540 550
QFSYPIQGGN QMMNQLGSPI GTQVGSPVGS QYGNQYGNQY SNQFGNQLQQ
560 570 580 590 600
QTSRPALHHG SNGEIRELTP SIVSSDSPDP AFFQDLQNNI DKQEESIQEI
610 620 630 640 650
QDWITKLNPG PGEDGNTPIF PELNMPSYFA NTGGSGQSEQ PSDYGDSQIE
660 670
ELRNSRLHEP DRSFEEKNNG QKRRRAA
Length:677
Mass (Da):75,420
Last modified:August 1, 1991 - v1
Checksum:i0BF03BCF990C210B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X55149 Genomic DNA. Translation: CAA38950.1.
CR382124 Genomic DNA. Translation: CAH00310.1.
PIRiS13365.
RefSeqiXP_453214.1. XM_453214.1.

Genome annotation databases

EnsemblFungiiCAH00310; CAH00310; KLLA0_D03322g.
GeneIDi2893291.
KEGGikla:KLLA0D03322g.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiHSF_KLULA
AccessioniPrimary (citable) accession number: P22121
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: August 1, 1991
Last modified: May 10, 2017
This is version 117 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families