Reviewed, UniProtKB/Swiss-Prot P22099 (TRPE_VIBPA)

Names and origin

Protein names

Recommended name:
    Anthranilate synthase component 1
    EC=4.1.3.27
Alternative name(s):
    Anthranilate synthase component I

Gene names

Name:	trpE
Ordered Locus Names:	VP1956

Organism

Vibrio parahaemolyticus [Complete proteome] [HAMAP]

Taxonomic identifier

670 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio

Protein attributes

Sequence length	541 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Chorismate + L-glutamine = anthranilate + pyruvate + L-glutamate.
Pathway	Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 1/5.
Subunit structure	Tetramer of two components I and two components II By similarity.
Miscellaneous	Component I catalyzes the formation of anthranilate using ammonia rather than glutamine, whereas component II provides glutamine amidotransferase activity.
Sequence similarities	Belongs to the anthranilate synthase component I family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis Tryptophan biosynthesis
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tryptophan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	anthranilate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 541	541	Anthranilate synthase component 1		PRO_0000154119
Experimental info
	Sequence conflict	105	1	V → L in CAA35031. Ref.1
	Sequence conflict	221	1	D → S in CAA35031. Ref.1
	Sequence conflict	233	1	E → A in CAA35031. Ref.1
	Sequence conflict	261	1	P → S in CAA35031. Ref.1
	Sequence conflict	366	1	S → G in CAA35031. Ref.1
	Sequence conflict	490	1	S → L in CAA35031. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P22099-1 [UniParc]. Last modified April 4, 2003. Version 2. Checksum: CE186C09CAB8AA0D	FASTA	541	59,771
	10 20 30 40 50 60 MRLCTAGRLK QLQGGLVNKA IEIKKLGQLE VLKASVPYTQ DPTRLFHTIC ENKTDSLLLE 70 80 90 100 110 120 SAEIDSKQNL KSLLIVDSAV RIVCYGHTVS FHALTENGKN LLTHVNQNVR GEVASQFDGE 130 140 150 160 170 180 TLTLEFIQPC DTIDEDSRLR EASSFDALRL VQHSFDLSSQ DKHAIFLGGL FAYDLVANFE 190 200 210 220 230 240 PLGDAVATNQ CPDYVFYVAE TLLVVDHQTE SCQLQATLFV DGSQKAALES RIEDIRAQCT 250 260 270 280 290 300 SPKRLPDATQ VANITAQPSV PDQDFCQIVR DLKEFVVKGD IFQVVPSRRF TLPCPSPLAA 310 320 330 340 350 360 YKELKQSNPS PYMFYMQDEL FTLFGASPES ALKYETDTNQ IEIYPIAGTR RRGKRPNGEI 370 380 390 400 410 420 DFDLDSRIEL ELRSDKKENA EHMMLVDLAR NDVARISQAG TRHVADLLKV DRYSHVMHLV 430 440 450 460 470 480 SRVVGQLRDD LDALHAYQAC MNMGTLTGAP KIRAMQLIRD VEGARRGSYG GAVGYLTGEG 490 500 510 520 530 540 TLDTCIVIRS AYVENGIAQV QAGAGVVFDS DPQAEADETR GKAQAVISAI QAAHSQPANK E

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and features of the tryptophan operon of Vibrio parahemolyticus." Crawford I.P., Han C.Y., Silverman M. DNA Seq. 1:189-196(1991) [PubMed: 1773058] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: BB22.
[2]	"Genome sequence of Vibrio parahaemolyticus: a pathogenic mechanism distinct from that of V. cholerae." Makino K., Oshima K., Kurokawa K., Yokoyama K., Uda T., Tagomori K., Iijima Y., Najima M., Nakano M., Yamashita A., Kubota Y., Kimura S., Yasunaga T., Honda T., Shinagawa H., Hattori M., Iida T. Lancet 361:743-749(2003) [PubMed: 12620739] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: RIMD 2210633 / Serotype O3:K6.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X17149 Genomic DNA. Translation: CAA35031.1. BA000031 Genomic DNA. Translation: BAC60219.1. Different initiation.
RefSeq	NP_798335.1.
3D structure databases
SMR	P22099. Positions 28-535.
ModBase	Search...
Genome annotation databases
GeneID	1189467.
GenomeReviews	Gene locus VP1956 in contig BA000031_GR.
KEGG	vpa:VP1956.
NMPDR	fig|223926.1.peg.1956.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG507440.
Enzyme and pathway databases
BioCyc	VPAR223926:VP1956-MONOMER.
BRENDA	4.1.3.27. 3063.
Family and domain databases
InterPro	IPR005801. ADC_synthase. IPR006805. Anth_synth_I_N. IPR019999. Anthranilate_synth_I_C. IPR015890. Chorismate-bd_C. IPR005257. TrpE_synth. [Graphical view]
Gene3D	G3DSA:3.60.120.10. TRPE_1_chor_bd. 1 hit.
PANTHER	PTHR11236. TRPE_1_chor_bd. 1 hit.
Pfam	PF04715. Anth_synt_I_N. 1 hit. PF00425. Chorismate_bind. 1 hit. [Graphical view]
PRINTS	PR00095. ANTSNTHASEI.
TIGRFAMs	TIGR00565. trpE_proteo. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TRPE_VIBPA

Accession

Primary (citable) accession number: P22099

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1991
Last sequence update:	April 4, 2003
Last modified:	February 9, 2010
This is version 73 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)