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P22091 (TAL1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 133. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
T-cell acute lymphocytic leukemia protein 1 homolog

Short name=TAL-1
Alternative name(s):
Stem cell protein
Gene names
Name:Tal1
Synonyms:Scl, Tal-1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length329 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Implicated in the genesis of hemopoietic malignancies. It may play an important role in hemopoietic differentiation. Serves as a positive regulator of erythroid differentiation. Ref.3

Subunit structure

Efficient DNA binding requires dimerization with another bHLH protein. Forms heterodimers with TCF3. Binds to the LIM domain containing protein LMO2 and to DRG1. Can assemble in a complex with LDB1 and LMO2. Component of a TAL-1 complex composed at least of CBFA2T3, LDB1, TAL1 and TCF3. Ref.3 Ref.5

Subcellular location

Nucleus Ref.3.

Tissue specificity

Erythroid and myeloid cells.

Domain

The helix-loop-helix domain is necessary and sufficient for the interaction with DRG1.

Post-translational modification

Phosphorylated on serine residues. Phosphorylation of Ser-122 by MAPK is strongly stimulated by hypoxia. Ref.4

Ubiquitinated; subsequent to hypoxia-dependent phosphorylation of Ser-122, ubiquitination targets the protein for rapid degradation via the ubiquitin system. This process may be characteristic for microvascular endothelial cells, since it could not be observed in large vessel endothelial cells. Ref.4

Involvement in disease

Involved in chromosomal translocation in leukemic stem-cells.

Sequence similarities

Contains 1 bHLH (basic helix-loop-helix) domain.

Ontologies

Keywords
   Biological processDifferentiation
Transcription
Transcription regulation
   Cellular componentNucleus
   Coding sequence diversityChromosomal rearrangement
   DiseaseProto-oncogene
   LigandDNA-binding
   Molecular functionDevelopmental protein
   PTMPhosphoprotein
Ubl conjugation
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processangiogenesis

Inferred from mutant phenotype PubMed 15677556PubMed 9472016. Source: MGI

astrocyte fate commitment

Inferred from mutant phenotype PubMed 16292311. Source: MGI

basophil differentiation

Inferred from electronic annotation. Source: Ensembl

cell fate commitment

Inferred from mutant phenotype PubMed 12569129. Source: MGI

definitive hemopoiesis

Inferred from mutant phenotype PubMed 18550854PubMed 8861941. Source: MGI

embryonic hemopoiesis

Inferred from mutant phenotype PubMed 19200805. Source: UniProtKB

erythrocyte differentiation

Inferred from direct assay PubMed 11731461PubMed 15314159. Source: MGI

erythrocyte maturation

Inferred from mutant phenotype PubMed 18550854. Source: MGI

generation of neurons

Inferred from mutant phenotype PubMed 16623824. Source: MGI

hemangioblast cell differentiation

Inferred from mutant phenotype PubMed 15677567PubMed 18550854PubMed 19182774. Source: MGI

hematopoietic stem cell differentiation

Inferred from mutant phenotype PubMed 14726374PubMed 15677556. Source: MGI

hemopoiesis

Inferred from direct assay PubMed 12485991. Source: MGI

locomotory behavior

Inferred from mutant phenotype PubMed 16623824. Source: MGI

megakaryocyte development

Inferred from mutant phenotype PubMed 16763211. Source: MGI

megakaryocyte differentiation

Inferred from mutant phenotype PubMed 12552125. Source: MGI

myeloid cell differentiation

Inferred from direct assay PubMed 14726394. Source: MGI

negative regulation of transcription from RNA polymerase II promoter

Inferred from genetic interaction PubMed 10373552PubMed 16007160PubMed 8760303. Source: MGI

neuron differentiation

Inferred from mutant phenotype PubMed 16292311. Source: MGI

platelet formation

Inferred from mutant phenotype PubMed 16763211. Source: MGI

positive regulation of cell division

Inferred from electronic annotation. Source: Ensembl

positive regulation of chromatin assembly or disassembly

Inferred from electronic annotation. Source: Ensembl

positive regulation of erythrocyte differentiation

Inferred from direct assay PubMed 19497860. Source: BHF-UCL

positive regulation of mitotic cell cycle

Inferred from electronic annotation. Source: Ensembl

positive regulation of protein complex assembly

Inferred from electronic annotation. Source: Ensembl

positive regulation of transcription from RNA polymerase II promoter

Inferred from genetic interaction PubMed 15314159PubMed 18184866PubMed 19323994PubMed 19799863PubMed 9819382. Source: MGI

positive regulation of transcription, DNA-templated

Inferred from sequence or structural similarity. Source: UniProtKB

regulation of cell proliferation

Inferred from mutant phenotype PubMed 20194619. Source: MGI

regulation of mast cell differentiation

Inferred from mutant phenotype PubMed 17644741. Source: MGI

regulation of myeloid cell differentiation

Inferred from mutant phenotype PubMed 16763211. Source: MGI

regulation of stem cell maintenance

Inferred from mutant phenotype PubMed 19850742. Source: MGI

regulation of transcription from RNA polymerase II promoter

Inferred from direct assay PubMed 19497860. Source: BHF-UCL

spinal cord association neuron differentiation

Inferred from genetic interaction PubMed 19323994. Source: MGI

transcription from RNA polymerase II promoter

Inferred from direct assay PubMed 18550854. Source: GOC

   Cellular_componentLsd1/2 complex

Inferred from electronic annotation. Source: Ensembl

histone deacetylase complex

Inferred from electronic annotation. Source: Ensembl

nuclear chromatin

Inferred from electronic annotation. Source: Ensembl

nucleus

Inferred from direct assay Ref.3. Source: UniProtKB

protein complex

Inferred from physical interaction Ref.3. Source: UniProtKB

transcription factor complex

Inferred from direct assay PubMed 8760303. Source: MGI

   Molecular_functionE-box binding

Inferred from direct assay PubMed 18550854. Source: MGI

RNA polymerase II core promoter sequence-specific DNA binding

Inferred from direct assay PubMed 23980096. Source: MGI

RNA polymerase II distal enhancer sequence-specific DNA binding

Inferred from direct assay PubMed 17962413. Source: MGI

chromatin binding

Inferred from direct assay PubMed 18184866PubMed 18550854PubMed 19011221. Source: MGI

protein binding

Inferred from physical interaction Ref.3. Source: UniProtKB

protein heterodimerization activity

Inferred from physical interaction PubMed 11439353PubMed 15314159PubMed 19323994. Source: MGI

sequence-specific DNA binding RNA polymerase II transcription factor activity

Inferred from direct assay PubMed 18550854. Source: MGI

transcription regulatory region DNA binding

Inferred from direct assay PubMed 19497860. Source: BHF-UCL

transcription regulatory region sequence-specific DNA binding

Inferred from direct assay PubMed 9111058. Source: MGI

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 329329T-cell acute lymphocytic leukemia protein 1 homolog
PRO_0000127455

Regions

Domain187 – 23953bHLH
Compositional bias263 – 27210Poly-Gly

Amino acid modifications

Modified residue1221Phosphoserine; by MAPK Ref.4

Experimental info

Mutagenesis1221S → A: Remains stable, even in the face of severe hypoxia. Ref.4

Sequences

Sequence LengthMass (Da)Tools
P22091 [UniParc].

Last modified August 1, 1991. Version 1.
Checksum: 189480B6B93CB371

FASTA32934,279
        10         20         30         40         50         60 
MTERPPSEAA RSDPQLEGQD AAEARMAPPH LVLLNGVAKE TSRAAPAEPP VIELGARSGA 

        70         80         90        100        110        120 
GGGPASGGGA ARDLKGRDAV AAEARLRVPT TELCRPPGPA PAPAPASAPA ELPGDGRMVQ 

       130        140        150        160        170        180 
LSPPALAAPA GPGRALLYSL SQPLASLGSG FFGEPDAFPM FTNNNRVKRR PSPYEMEISD 

       190        200        210        220        230        240 
GPHTKVVRRI FTNSRERWRQ QNVNGAFAEL RKLIPTHPPD KKLSKNEILR LAMKYINFLA 

       250        260        270        280        290        300 
KLLNDQEEEG TQRAKPGKDP VVGAGGGGAG GGIPPEDLLQ DVLSPNSSCG SSLDGAASPD 

       310        320 
SYTEEPTPKH TSRSLHPALL PAADGAGPR 

« Hide

References

« Hide 'large scale' references
[1]"Molecular cloning and chromosomal localization of the murine homolog of the human helix-loop-helix gene SCL."
Begley C.G., Visvader J., Green A.R., Aplan P.D., Metcalf D., Kirsch I.R., Gough N.M.
Proc. Natl. Acad. Sci. U.S.A. 88:869-873(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Bone marrow macrophage.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6.
Tissue: Brain.
[3]"The LIM-domain binding protein Ldb1 and its partner LMO2 act as negative regulators of erythroid differentiation."
Visvader J.E., Mao X., Fujiwara Y., Hahm K., Orkin S.H.
Proc. Natl. Acad. Sci. U.S.A. 94:13707-13712(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH LMO2, IDENTIFICATION IN A COMPLEX WITH LDB1 AND LMO2, SUBCELLULAR LOCATION.
[4]"Phosphorylation by mitogen-activated protein kinase mediates the hypoxia-induced turnover of the TAL1/SCL transcription factor in endothelial cells."
Tang T., Arbiser J.L., Brandt S.J.
J. Biol. Chem. 277:18365-18372(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION AT SER-122, MUTAGENESIS OF SER-122, UBIQUITINATION.
[5]"ETO2 coordinates cellular proliferation and differentiation during erythropoiesis."
Goardon N., Lambert J.A., Rodriguez P., Nissaire P., Herblot S., Thibault P., Dumenil D., Strouboulis J., Romeo P.-H., Hoang T.
EMBO J. 25:357-366(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH CBFA2T3.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M59764 mRNA. Translation: AAA40097.1.
U01530 Genomic DNA. Translation: AAA86937.1.
BC063060 mRNA. Translation: AAH63060.1.
CCDSCCDS18486.1.
PIRA37864.
RefSeqNP_001274317.1. NM_001287388.1.
NP_035657.1. NM_011527.3.
XP_006502973.1. XM_006502910.1.
XP_006502975.1. XM_006502912.1.
XP_006502976.1. XM_006502913.1.
XP_006502977.1. XM_006502914.1.
XP_006502978.1. XM_006502915.1.
XP_006502979.1. XM_006502916.1.
UniGeneMm.439685.

3D structure databases

ProteinModelPortalP22091.
SMRP22091. Positions 182-248.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid203962. 11 interactions.
DIPDIP-42839N.
IntActP22091. 18 interactions.
MINTMINT-2567683.
STRING10090.ENSMUSP00000030489.

PTM databases

PhosphoSiteP22091.

Proteomic databases

PaxDbP22091.
PRIDEP22091.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000030489; ENSMUSP00000030489; ENSMUSG00000028717.
ENSMUST00000161601; ENSMUSP00000125202; ENSMUSG00000028717.
ENSMUST00000162489; ENSMUSP00000124983; ENSMUSG00000028717.
GeneID21349.
KEGGmmu:21349.
UCSCuc008uek.1. mouse.

Organism-specific databases

CTD6886.
MGIMGI:98480. Tal1.

Phylogenomic databases

eggNOGNOG307510.
HOGENOMHOG000113414.
HOVERGENHBG005018.
InParanoidP22091.
KOK09068.
OMAEGSQRAK.
OrthoDBEOG7SN8DF.
PhylomeDBP22091.
TreeFamTF315153.

Gene expression databases

ArrayExpressP22091.
BgeeP22091.
CleanExMM_TAL1.
GenevestigatorP22091.

Family and domain databases

Gene3D4.10.280.10. 1 hit.
InterProIPR011598. bHLH_dom.
[Graphical view]
PfamPF00010. HLH. 1 hit.
[Graphical view]
SMARTSM00353. HLH. 1 hit.
[Graphical view]
SUPFAMSSF47459. SSF47459. 1 hit.
PROSITEPS50888. BHLH. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio300536.
PROP22091.
SOURCESearch...

Entry information

Entry nameTAL1_MOUSE
AccessionPrimary (citable) accession number: P22091
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: August 1, 1991
Last modified: July 9, 2014
This is version 133 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot