P22083 (FUT4_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alpha-(1,3)-fucosyltransferase
EC=2.4.1.-

Alternative name(s):
ELAM-1 ligand fucosyltransferase
Fucosyltransferase 4
Fucosyltransferase IV
Short name=Fuc-TIV
Short name=FucT-IV
Galactoside 3-L-fucosyltransferase

Gene names

Name:	FUT4
Synonyms:	ELFT, FCT3A

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	530 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May catalyze alpha-1,3 glycosidic linkages involved in the expression of Lewis X/SSEA-1 and VIM-2 antigens.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus › Golgi stack membrane; Single-pass type II membrane protein. Note: Membrane-bound form in trans cisternae of Golgi.
Sequence similarities	Belongs to the glycosyltransferase 10 family.
Caution	It is uncertain whether Met-1 or Met-126 is the initiator.
Sequence caution	The sequence AAB20349.1 differs from that shown. Reason: Frameshift at position 114.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Coding sequence diversity	Polymorphism
Domain	Signal-anchor Transmembrane Transmembrane helix
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	L-fucose catabolic process Non-traceable author statement PubMed 11698403. Source: UniProtKB protein glycosylation Traceable author statement PubMed 11698403. Source: UniProtKB
Cellular_component	Golgi apparatus Traceable author statement PubMed 11698403. Source: UniProtKB Golgi cisterna membrane Inferred from electronic annotation. Source: UniProtKB-SubCell cell periphery Inferred from electronic annotation. Source: Compara cell surface Inferred from electronic annotation. Source: Compara integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW membrane Traceable author statement Ref.6. Source: ProtInc
Molecular_function	alpha-(1->3)-fucosyltransferase activity Traceable author statement PubMed 11698403. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 530	530	Alpha-(1,3)-fucosyltransferase		PRO_0000221100
Regions
	Topological domain	1 – 147	147	Cytoplasmic Potential
	Transmembrane	148 – 172	25	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	173 – 530	358	Lumenal Potential
Amino acid modifications
	Glycosylation	216	1	N-linked (GlcNAc...) Potential
	Glycosylation	315	1	N-linked (GlcNAc...) Potential
Natural variations
	Natural variant	255	1	I → V. Corresponds to variant rs2230273 [ dbSNP | Ensembl ].		VAR_055844
Experimental info
	Sequence conflict	212	1	R → P in AAA92977. Ref.6
	Sequence conflict	366	1	E → D in AAB20349. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	P22083 [UniParc]. Last modified April 14, 2009. Version 3. Checksum: B10C1C3C67BE1562	FASTA	530	59,084
	10 20 30 40 50 60 MRRLWGAARK PSGAGWEKEW AEAPQEAPGA WSGRLGPGRS GRKGRAVPGW ASWPAHLALA 70 80 90 100 110 120 ARPARHLGGA GQGPRPLHSG TAPFHSRASG ERQRRLEPQL QHESRCRSST PADAWRAEAA 130 140 150 160 170 180 LPVRAMGAPW GSPTAAAGGR RGWRRGRGLP WTVCVLAAAG LTCTALITYA CWGQLPPLPW 190 200 210 220 230 240 ASPTPSRPVG VLLWWEPFGG RDSAPRPPPD CRLRFNISGC RLLTDRASYG EAQAVLFHHR 250 260 270 280 290 300 DLVKGPPDWP PPWGIQAHTA EEVDLRVLDY EEAAAAAEAL ATSSPRPPGQ RWVWMNFESP 310 320 330 340 350 360 SHSPGLRSLA SNLFNWTLSY RADSDVFVPY GYLYPRSHPG DPPSGLAPPL SRKQGLVAWV 370 380 390 400 410 420 VSHWDERQAR VRYYHQLSQH VTVDVFGRGG PGQPVPEIGL LHTVARYKFY LAFENSQHLD 430 440 450 460 470 480 YITEKLWRNA LLAGAVPVVL GPDRANYERF VPRGAFIHVD DFPSASSLAS YLLFLDRNPA 490 500 510 520 530 VYRRYFHWRR SYAVHITSFW DEPWCRVCQA VQRAGDRPKS IRNLASWFER

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"ELFT: a gene that directs the expression of an ELAM-1 ligand." Goelz S.E., Hession C., Goff D., Griffiths B., Tizard R., Newman B., Chi-Rosso G., Lobb R. Cell 63:1349-1356(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Human chromosome 11 DNA sequence and analysis including novel gene identification." Taylor T.D., Noguchi H., Totoki Y., Toyoda A., Kuroki Y., Dewar K., Lloyd C., Itoh T., Takeda T., Kim D.-W., She X., Barlow K.F., Bloom T., Bruford E., Chang J.L., Cuomo C.A., Eichler E., FitzGerald M.G. , Jaffe D.B., LaButti K., Nicol R., Park H.-S., Seaman C., Sougnez C., Yang X., Zimmer A.R., Zody M.C., Birren B.W., Nusbaum C., Fujiyama A., Hattori M., Rogers J., Lander E.S., Sakaki Y. Nature 440:497-500(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis.
[5]	"Cloning of a human alpha(1,3)-fucosyltransferase gene that encodes ELFT but does not confer ELAM-1 recognition on Chinese hamster ovary cell transfectants." Kumar R., Potvin B., Muller W.A., Stanley P. J. Biol. Chem. 266:21777-21783(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 77-525.
[6]	"Molecular cloning of a human fucosyltransferase gene that determines expression of the Lewis x and VIM-2 epitopes but not ELAM-1-dependent cell adhesion." Lowe J.B., Kukowska-Latallo J.F., Nair R.P., Larsen R.D., Marks R.M., Macher B.A., Kelly R.J., Ernst L.K. J. Biol. Chem. 266:17467-17477(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 114-530. Tissue: Peripheral blood leukocyte.
+	Additional computationally mapped references.

Web resources

GGDB

GlycoGene database

Functional Glycomics Gateway - GTase

Fucosyltransferase 4

Cross-references

Sequence databases
EMBL GenBank DDBJ	M58596 mRNA. Translation: AAA63172.1. M58597 mRNA. Translation: AAA63173.1. AP000943 Genomic DNA. No translation available. CH471065 Genomic DNA. Translation: EAW66938.1. BC136373 mRNA. Translation: AAI36374.1. BC136374 mRNA. Translation: AAI36375.1. S65161 Genomic DNA. Translation: AAB20349.1. Sequence problems. M65030 Genomic DNA. Translation: AAA92977.1.
IPI	IPI00219011.
PIR	B36340.
RefSeq	NP_002024.1. NM_002033.3.
UniGene	Hs.390420. Hs.572064. Hs.623098.
3D structure databases
ProteinModelPortal	P22083.
SMR	P22083. Positions 352-494.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000351602.
Protein family/group databases
CAZy	GT10. Glycosyltransferase Family 10.
PTM databases
PhosphoSite	P22083.
Polymorphism databases
DMDM	226694189.
Proteomic databases
PaxDb	P22083.
PRIDE	P22083.
Protocols and materials databases
DNASU	2526.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000358752; ENSP00000351602; ENSG00000196371.
GeneID	2526.
KEGG	hsa:2526.
UCSC	uc001pez.3. human.
Organism-specific databases
CTD	2526.
GeneCards	GC11P094277.
H-InvDB	HIX0036409.
HGNC	HGNC:4015. FUT4.
HPA	CAB000014. CAB016129.
MIM	104230. gene.
neXtProt	NX_P22083.
PharmGKB	PA28431.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG283180.
HOGENOM	HOG000045583.
HOVERGEN	HBG000274.
InParanoid	P22083.
KO	K07632.
OMA	CRLRFNI.
OrthoDB	EOG4H72BB.
PhylomeDB	P22083.
Enzyme and pathway databases
UniPathway	UPA00378.
Gene expression databases
Bgee	P22083.
CleanEx	HS_FUT4.
Genevestigator	P22083.
GermOnline	ENSG00000196371. Homo sapiens.
Family and domain databases
InterPro	IPR001503. Glyco_trans_10. [Graphical view]
PANTHER	PTHR11929. PTHR11929. 1 hit.
Pfam	PF00852. Glyco_transf_10. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChEMBL	CHEMBL4996.
GenomeRNAi	2526.
NextBio	9953.
SOURCE	Search...

Entry information

Entry name

FUT4_HUMAN

Accession

Primary (citable) accession number: P22083
Secondary accession number(s): B2RMS0

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1991
Last sequence update:	April 14, 2009
Last modified:	May 1, 2013
This is version 123 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.