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Protein

Alpha-(1,3)-fucosyltransferase 4

Gene

FUT4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May catalyze alpha-1,3 glycosidic linkages involved in the expression of Lewis X/SSEA-1 and VIM-2 antigens.

Pathwayi

GO - Molecular functioni

  1. alpha-(1->3)-fucosyltransferase activity Source: UniProtKB
  2. fucosyltransferase activity Source: ProtInc

GO - Biological processi

  1. carbohydrate metabolic process Source: ProtInc
  2. fucosylation Source: GOC
  3. L-fucose catabolic process Source: UniProtKB
  4. protein glycosylation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT10. Glycosyltransferase Family 10.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-(1,3)-fucosyltransferase 4 (EC:2.4.1.-)
Alternative name(s):
ELAM-1 ligand fucosyltransferase
Fucosyltransferase 4
Fucosyltransferase IV
Short name:
Fuc-TIV
Short name:
FucT-IV
Galactoside 3-L-fucosyltransferase
Gene namesi
Name:FUT4
Synonyms:ELFT, FCT3A
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 11

Organism-specific databases

HGNCiHGNC:4015. FUT4.

Subcellular locationi

Golgi apparatusGolgi stack membrane; Single-pass type II membrane protein
Note: Membrane-bound form in trans cisternae of Golgi.

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 147147CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei148 – 17225Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
BLAST
Topological domaini173 – 530358LumenalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. cell periphery Source: Ensembl
  2. cell surface Source: Ensembl
  3. Golgi apparatus Source: UniProtKB
  4. Golgi cisterna membrane Source: UniProtKB-SubCell
  5. integral component of membrane Source: UniProtKB-KW
  6. membrane Source: ProtInc
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA28431.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 530530Alpha-(1,3)-fucosyltransferase 4PRO_0000221100Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi216 – 2161N-linked (GlcNAc...)Sequence Analysis
Glycosylationi315 – 3151N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiP22083.
PaxDbiP22083.
PRIDEiP22083.

PTM databases

PhosphoSiteiP22083.

Expressioni

Gene expression databases

BgeeiP22083.
CleanExiHS_FUT4.
GenevestigatoriP22083.

Organism-specific databases

HPAiCAB000014.
CAB016129.

Interactioni

Protein-protein interaction databases

BioGridi108802. 2 interactions.
STRINGi9606.ENSP00000351602.

Structurei

3D structure databases

ProteinModelPortaliP22083.
SMRiP22083. Positions 352-494.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyltransferase 10 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG283180.
GeneTreeiENSGT00680000099679.
HOGENOMiHOG000045583.
HOVERGENiHBG000274.
InParanoidiP22083.
KOiK07632.
OMAiCRLRFNI.
OrthoDBiEOG7Z69C9.
PhylomeDBiP22083.
TreeFamiTF316348.

Family and domain databases

InterProiIPR001503. Glyco_trans_10.
[Graphical view]
PANTHERiPTHR11929. PTHR11929. 1 hit.
PfamiPF00852. Glyco_transf_10. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P22083-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRRLWGAARK PSGAGWEKEW AEAPQEAPGA WSGRLGPGRS GRKGRAVPGW
60 70 80 90 100
ASWPAHLALA ARPARHLGGA GQGPRPLHSG TAPFHSRASG ERQRRLEPQL
110 120 130 140 150
QHESRCRSST PADAWRAEAA LPVRAMGAPW GSPTAAAGGR RGWRRGRGLP
160 170 180 190 200
WTVCVLAAAG LTCTALITYA CWGQLPPLPW ASPTPSRPVG VLLWWEPFGG
210 220 230 240 250
RDSAPRPPPD CRLRFNISGC RLLTDRASYG EAQAVLFHHR DLVKGPPDWP
260 270 280 290 300
PPWGIQAHTA EEVDLRVLDY EEAAAAAEAL ATSSPRPPGQ RWVWMNFESP
310 320 330 340 350
SHSPGLRSLA SNLFNWTLSY RADSDVFVPY GYLYPRSHPG DPPSGLAPPL
360 370 380 390 400
SRKQGLVAWV VSHWDERQAR VRYYHQLSQH VTVDVFGRGG PGQPVPEIGL
410 420 430 440 450
LHTVARYKFY LAFENSQHLD YITEKLWRNA LLAGAVPVVL GPDRANYERF
460 470 480 490 500
VPRGAFIHVD DFPSASSLAS YLLFLDRNPA VYRRYFHWRR SYAVHITSFW
510 520 530
DEPWCRVCQA VQRAGDRPKS IRNLASWFER
Length:530
Mass (Da):59,084
Last modified:April 14, 2009 - v3
Checksum:iB10C1C3C67BE1562
GO

Sequence cautioni

The sequence AAB20349.1 differs from that shown. Reason: Frameshift at position 114. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti212 – 2121R → P in AAA92977 (PubMed:1716630).Curated
Sequence conflicti366 – 3661E → D in AAB20349 (PubMed:1718983).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti255 – 2551I → V.
Corresponds to variant rs2230273 [ dbSNP | Ensembl ].
VAR_055844

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M58596 mRNA. Translation: AAA63172.1.
M58597 mRNA. Translation: AAA63173.1.
AP000943 Genomic DNA. No translation available.
CH471065 Genomic DNA. Translation: EAW66938.1.
BC136373 mRNA. Translation: AAI36374.1.
BC136374 mRNA. Translation: AAI36375.1.
S65161 Genomic DNA. Translation: AAB20349.1. Sequence problems.
M65030 Genomic DNA. Translation: AAA92977.1.
CCDSiCCDS8301.1.
PIRiB36340.
RefSeqiNP_002024.1. NM_002033.3.
UniGeneiHs.390420.
Hs.572064.
Hs.623098.

Genome annotation databases

EnsembliENST00000358752; ENSP00000351602; ENSG00000196371.
GeneIDi2526.
KEGGihsa:2526.
UCSCiuc001pez.3. human.

Polymorphism databases

DMDMi226694189.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Web resourcesi

GGDB

GlycoGene database

Functional Glycomics Gateway - GTase

Fucosyltransferase 4

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M58596 mRNA. Translation: AAA63172.1.
M58597 mRNA. Translation: AAA63173.1.
AP000943 Genomic DNA. No translation available.
CH471065 Genomic DNA. Translation: EAW66938.1.
BC136373 mRNA. Translation: AAI36374.1.
BC136374 mRNA. Translation: AAI36375.1.
S65161 Genomic DNA. Translation: AAB20349.1. Sequence problems.
M65030 Genomic DNA. Translation: AAA92977.1.
CCDSiCCDS8301.1.
PIRiB36340.
RefSeqiNP_002024.1. NM_002033.3.
UniGeneiHs.390420.
Hs.572064.
Hs.623098.

3D structure databases

ProteinModelPortaliP22083.
SMRiP22083. Positions 352-494.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi108802. 2 interactions.
STRINGi9606.ENSP00000351602.

Chemistry

ChEMBLiCHEMBL4996.

Protein family/group databases

CAZyiGT10. Glycosyltransferase Family 10.

PTM databases

PhosphoSiteiP22083.

Polymorphism databases

DMDMi226694189.

Proteomic databases

MaxQBiP22083.
PaxDbiP22083.
PRIDEiP22083.

Protocols and materials databases

DNASUi2526.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000358752; ENSP00000351602; ENSG00000196371.
GeneIDi2526.
KEGGihsa:2526.
UCSCiuc001pez.3. human.

Organism-specific databases

CTDi2526.
GeneCardsiGC11P094277.
H-InvDBHIX0036409.
HGNCiHGNC:4015. FUT4.
HPAiCAB000014.
CAB016129.
MIMi104230. gene.
neXtProtiNX_P22083.
PharmGKBiPA28431.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG283180.
GeneTreeiENSGT00680000099679.
HOGENOMiHOG000045583.
HOVERGENiHBG000274.
InParanoidiP22083.
KOiK07632.
OMAiCRLRFNI.
OrthoDBiEOG7Z69C9.
PhylomeDBiP22083.
TreeFamiTF316348.

Enzyme and pathway databases

UniPathwayiUPA00378.

Miscellaneous databases

GenomeRNAii2526.
NextBioi9953.
PROiP22083.
SOURCEiSearch...

Gene expression databases

BgeeiP22083.
CleanExiHS_FUT4.
GenevestigatoriP22083.

Family and domain databases

InterProiIPR001503. Glyco_trans_10.
[Graphical view]
PANTHERiPTHR11929. PTHR11929. 1 hit.
PfamiPF00852. Glyco_transf_10. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "ELFT: a gene that directs the expression of an ELAM-1 ligand."
    Goelz S.E., Hession C., Goff D., Griffiths B., Tizard R., Newman B., Chi-Rosso G., Lobb R.
    Cell 63:1349-1356(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Testis.
  5. "Cloning of a human alpha(1,3)-fucosyltransferase gene that encodes ELFT but does not confer ELAM-1 recognition on Chinese hamster ovary cell transfectants."
    Kumar R., Potvin B., Muller W.A., Stanley P.
    J. Biol. Chem. 266:21777-21783(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 77-525.
  6. "Molecular cloning of a human fucosyltransferase gene that determines expression of the Lewis x and VIM-2 epitopes but not ELAM-1-dependent cell adhesion."
    Lowe J.B., Kukowska-Latallo J.F., Nair R.P., Larsen R.D., Marks R.M., Macher B.A., Kelly R.J., Ernst L.K.
    J. Biol. Chem. 266:17467-17477(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 114-530.
    Tissue: Peripheral blood leukocyte.

Entry informationi

Entry nameiFUT4_HUMAN
AccessioniPrimary (citable) accession number: P22083
Secondary accession number(s): B2RMS0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: April 14, 2009
Last modified: January 7, 2015
This is version 136 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Caution

It is uncertain whether Met-1 or Met-126 is the initiator.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  6. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.