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Protein

Transcription regulatory protein SNF2

Gene

SNF2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in transcriptional activation. Catalytic component of the SWI/SNF complex, an ATP-dependent chromatin-remodeling complex, which is required for the positive and negative regulation of gene expression of a large number of genes. It changes chromatin structure by altering DNA-histone contacts within a nucleosome, leading eventually to a change in nucleosome position, thus facilitating or repressing binding of gene-specific transcription factors.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi792 – 799ATPPROSITE-ProRule annotation8
DNA bindingi1446 – 1456A.T hook 1Add BLAST11
DNA bindingi1502 – 1513A.T hook 2Add BLAST12
DNA bindingi1516 – 1526A.T hook 3Add BLAST11

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • DNA-dependent ATPase activity Source: SGD
  • helicase activity Source: UniProtKB-KW
  • lysine-acetylated histone binding Source: SGD
  • nucleosomal DNA binding Source: SGD
  • rDNA binding Source: SGD
  • RNA polymerase II activating transcription factor binding Source: SGD

GO - Biological processi

  • ATP-dependent chromatin remodeling Source: SGD
  • cellular alcohol catabolic process Source: SGD
  • chromatin remodeling Source: SGD
  • DNA-dependent DNA replication Source: SGD
  • double-strand break repair Source: SGD
  • nucleosome mobilization Source: SGD
  • positive regulation of cell adhesion involved in single-species biofilm formation Source: SGD
  • positive regulation of invasive growth in response to glucose limitation Source: SGD
  • positive regulation of mating type switching Source: SGD
  • positive regulation of transcription from RNA polymerase II promoter Source: SGD
  • positive regulation of transcription from RNA polymerase II promoter in response to amino acid starvation Source: SGD
  • strand invasion Source: SGD
  • sucrose catabolic process Source: SGD
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator, Helicase, Hydrolase

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-33775-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription regulatory protein SNF2 (EC:3.6.4.-)
Alternative name(s):
ATP-dependent helicase SNF2
Regulatory protein GAM1
Regulatory protein SWI2
SWI/SNF complex component SNF2
Transcription factor TYE3
Gene namesi
Name:SNF2
Synonyms:GAM1, RIC1, SWI2, TYE3
Ordered Locus Names:YOR290C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOR290C.
SGDiS000005816. SNF2.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: SGD
  • SWI/SNF complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000743581 – 1703Transcription regulatory protein SNF2Add BLAST1703

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei358PhosphoserineCombined sources1
Modified residuei383PhosphothreonineCombined sources1
Cross-linki543Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Modified residuei716PhosphoserineCombined sources1
Modified residuei1340PhosphoserineCombined sources1

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP22082.
PRIDEiP22082.

PTM databases

iPTMnetiP22082.

Interactioni

Subunit structurei

Component of the SWI/SNF global transcription activator complex. The 1.14 MDa SWI/SNF complex is composed of 11 different subunits: one copy each of SWI1, SNF2/SWI2, SNF5, SNF12/SWP73, ARP7/SWP61, ARP9/SWP59; two copies each of SWI3, SNF6, SNF11, SWP82; and three copies of TAF14/SWP29.

Binary interactionsi

WithEntry#Exp.IntActNotes
HMT1P380743EBI-17526,EBI-8394
SNF11P389563EBI-17526,EBI-17560
SWI3P325916EBI-17526,EBI-18622

GO - Molecular functioni

  • lysine-acetylated histone binding Source: SGD
  • RNA polymerase II activating transcription factor binding Source: SGD

Protein-protein interaction databases

BioGridi34678. 157 interactors.
DIPiDIP-1150N.
IntActiP22082. 43 interactors.
MINTiMINT-600461.

Structurei

Secondary structure

11703
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi593 – 657Combined sources65

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4I6MX-ray2.80C575-667[»]
ProteinModelPortaliP22082.
SMRiP22082.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini247 – 282QLQPROSITE-ProRule annotationAdd BLAST36
Domaini588 – 661HSAPROSITE-ProRule annotationAdd BLAST74
Domaini779 – 944Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST166
Domaini1091 – 1254Helicase C-terminalPROSITE-ProRule annotationAdd BLAST164
Domaini1568 – 1638BromoPROSITE-ProRule annotationAdd BLAST71

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi894 – 897DEGH box4

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi55 – 68Gln-richAdd BLAST14
Compositional biasi207 – 239Ala/Gln-richAdd BLAST33

Sequence similaritiesi

Contains 3 A.T hook DNA-binding domains.Curated
Contains 1 bromo domain.PROSITE-ProRule annotation
Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation
Contains 1 HSA domain.PROSITE-ProRule annotation
Contains 1 QLQ domain.PROSITE-ProRule annotation

Keywords - Domaini

Bromodomain, Repeat

Phylogenomic databases

GeneTreeiENSGT00550000074659.
HOGENOMiHOG000172362.
InParanoidiP22082.
KOiK11786.
OMAiNRCYLRW.
OrthoDBiEOG092C1YH4.

Family and domain databases

Gene3Di1.20.920.10. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR017956. AT_hook_DNA-bd_motif.
IPR001487. Bromodomain.
IPR018359. Bromodomain_CS.
IPR014978. Gln-Leu-Gln_QLQ.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR014012. HSA_dom.
IPR027417. P-loop_NTPase.
IPR029295. SnAC.
IPR000330. SNF2_N.
[Graphical view]
PfamiPF00439. Bromodomain. 1 hit.
PF00271. Helicase_C. 1 hit.
PF07529. HSA. 1 hit.
PF08880. QLQ. 1 hit.
PF14619. SnAC. 1 hit.
PF00176. SNF2_N. 1 hit.
[Graphical view]
PRINTSiPR00503. BROMODOMAIN.
SMARTiSM00384. AT_hook. 2 hits.
SM00297. BROMO. 1 hit.
SM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
SM00951. QLQ. 1 hit.
SM01314. SnAC. 1 hit.
[Graphical view]
SUPFAMiSSF47370. SSF47370. 1 hit.
SSF52540. SSF52540. 2 hits.
PROSITEiPS00633. BROMODOMAIN_1. 1 hit.
PS50014. BROMODOMAIN_2. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51204. HSA. 1 hit.
PS51666. QLQ. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P22082-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNIPQRQFSN EEVNRCYLRW QHLRNEHGMN APSVPEFIYL TKVLQFAAKQ
60 70 80 90 100
RQELQMQRQQ QGISGSQQNI VPNSSDQAEL PNNASSHISA SASPHLAPNM
110 120 130 140 150
QLNGNETFST SAHQSPIMQT QMPLNSNGGN NMLPQRQSSV GSLNATNFSP
160 170 180 190 200
TPANNGENAA EKPDNSNHNN LNLNNSELQP QNRSLQEHNI QDSNVMPGSQ
210 220 230 240 250
INSPMPQQAQ MQQAQFQAQQ AQQAQQAQQA QQAQARLQQG RRLPMTMFTA
260 270 280 290 300
EQSELLKAQI TSLKCLVNRK PIPFEFQAVI QKSINHPPDF KRMLLSLSEF
310 320 330 340 350
ARRRQPTDQN NQSNLNGGNN TQQPGTNSHY NNTNTDNVSG LTRNAPLDSK
360 370 380 390 400
DENFASVSPA GPSSVHNAKN GTLDKNSQTV SGTPITQTES KKEENETISN
410 420 430 440 450
VAKTAPNSNK THTEQNNPPK PQKPVPLNVL QDQYKEGIKV VDIDDPDMMV
460 470 480 490 500
DSFTMPNISH SNIDYQTLLA NSDHAKFTIE PGVLPVGIDT HTATDIYQTL
510 520 530 540 550
IALNLDTTVN DCLDKLLNDE CTESTRENAL YDYYALQLLP LQKAVRGHVL
560 570 580 590 600
QFEWHQNSLL TNTHPNFLSK IRNINVQDAL LTNQLYKNHE LLKLERKKTE
610 620 630 640 650
AVARLKSMNK SAINQYNRRQ DKKNKRLKFG HRLIATHTNL ERDEQKRAEK
660 670 680 690 700
KAKERLQALK ANDEEAYIKL LDQTKDTRIT HLLRQTNAFL DSLTRAVKDQ
710 720 730 740 750
QKYTKEMIDS HIKEASEEVD DLSMVPKMKD EEYDDDDDNS NVDYYNVAHR
760 770 780 790 800
IKEDIKKQPS ILVGGTLKDY QIKGLQWMVS LFNNHLNGIL ADEMGLGKTI
810 820 830 840 850
QTISLLTYLY EMKNIRGPYL VIVPLSTLSN WSSEFAKWAP TLRTISFKGS
860 870 880 890 900
PNERKAKQAK IRAGEFDVVL TTFEYIIKER ALLSKVKWVH MIIDEGHRMK
910 920 930 940 950
NAQSKLSLTL NTHYHADYRL ILTGTPLQNN LPELWALLNF VLPKIFNSVK
960 970 980 990 1000
SFDEWFNTPF ANTGGQDKIE LSEEETLLVI RRLHKVLRPF LLRRLKKDVE
1010 1020 1030 1040 1050
KELPDKVEKV VKCKMSALQQ IMYQQMLKYR RLFIGDQNNK KMVGLRGFNN
1060 1070 1080 1090 1100
QIMQLKKICN HPFVFEEVED QINPTRETND DIWRVAGKFE LLDRILPKLK
1110 1120 1130 1140 1150
ATGHRVLIFF QMTQIMDIME DFLRYINIKY LRLDGHTKSD ERSELLRLFN
1160 1170 1180 1190 1200
APDSEYLCFI LSTRAGGLGL NLQTADTVII FDTDWNPHQD LQAQDRAHRI
1210 1220 1230 1240 1250
GQKNEVRILR LITTNSVEEV ILERAYKKLD IDGKVIQAGK FDNKSTSEEQ
1260 1270 1280 1290 1300
EALLRSLLDA EEERRKKRES GVEEEEELKD SEINEILARN DEEMAVLTRM
1310 1320 1330 1340 1350
DEDRSKKEEE LGVKSRLLEK SELPDIYSRD IGAELKREES ESAAVYNGRG
1360 1370 1380 1390 1400
ARERKTATYN DNMSEEQWLR QFEVSDDEKN DKQARKQRTK KEDKSEAIDG
1410 1420 1430 1440 1450
NGEIKGENID ADNDGPRINN ISAEDRADTD LAMNDDDFLS KKRKAGRPRG
1460 1470 1480 1490 1500
RPKKVKLEGS ENSEPPALES SPVTGDNSPS EDFMDIPKPR TAGKTSVKSA
1510 1520 1530 1540 1550
RTSTRGRGRG RGRGRGRGRG RGRPPKARNG LDYVRTPAAA TSPIDIREKV
1560 1570 1580 1590 1600
AKQALDLYHF ALNYENEAGR KLSDIFLSKP SKALYPDYYM IIKYPVAFDN
1610 1620 1630 1640 1650
INTHIETLAY NSLKETLQDF HLIFSNARIY NTEGSVVYED SLELEKVVTK
1660 1670 1680 1690 1700
KYCEIMGDNS QLDFTEFDEQ YGTRPLVLPP VVTSSVAESF TDEADSSMTE

ASV
Length:1,703
Mass (Da):194,051
Last modified:August 1, 1991 - v1
Checksum:i84B8BC595C8F3E6D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M61703 Genomic DNA. Translation: AAA35059.1.
X57837 Genomic DNA. Translation: CAA40969.1.
D90459 Genomic DNA. Translation: BAA14423.1.
X89633 Genomic DNA. Translation: CAA61793.1.
Z75198 Genomic DNA. Translation: CAA99517.1.
Z75199 Genomic DNA. Translation: CAA99519.1.
BK006948 Genomic DNA. Translation: DAA11054.1.
PIRiS15047.
RefSeqiNP_014933.3. NM_001183709.3.

Genome annotation databases

EnsemblFungiiYOR290C; YOR290C; YOR290C.
GeneIDi854465.
KEGGisce:YOR290C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M61703 Genomic DNA. Translation: AAA35059.1.
X57837 Genomic DNA. Translation: CAA40969.1.
D90459 Genomic DNA. Translation: BAA14423.1.
X89633 Genomic DNA. Translation: CAA61793.1.
Z75198 Genomic DNA. Translation: CAA99517.1.
Z75199 Genomic DNA. Translation: CAA99519.1.
BK006948 Genomic DNA. Translation: DAA11054.1.
PIRiS15047.
RefSeqiNP_014933.3. NM_001183709.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4I6MX-ray2.80C575-667[»]
ProteinModelPortaliP22082.
SMRiP22082.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34678. 157 interactors.
DIPiDIP-1150N.
IntActiP22082. 43 interactors.
MINTiMINT-600461.

PTM databases

iPTMnetiP22082.

Proteomic databases

MaxQBiP22082.
PRIDEiP22082.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYOR290C; YOR290C; YOR290C.
GeneIDi854465.
KEGGisce:YOR290C.

Organism-specific databases

EuPathDBiFungiDB:YOR290C.
SGDiS000005816. SNF2.

Phylogenomic databases

GeneTreeiENSGT00550000074659.
HOGENOMiHOG000172362.
InParanoidiP22082.
KOiK11786.
OMAiNRCYLRW.
OrthoDBiEOG092C1YH4.

Enzyme and pathway databases

BioCyciYEAST:G3O-33775-MONOMER.

Miscellaneous databases

PROiP22082.

Family and domain databases

Gene3Di1.20.920.10. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR017956. AT_hook_DNA-bd_motif.
IPR001487. Bromodomain.
IPR018359. Bromodomain_CS.
IPR014978. Gln-Leu-Gln_QLQ.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR014012. HSA_dom.
IPR027417. P-loop_NTPase.
IPR029295. SnAC.
IPR000330. SNF2_N.
[Graphical view]
PfamiPF00439. Bromodomain. 1 hit.
PF00271. Helicase_C. 1 hit.
PF07529. HSA. 1 hit.
PF08880. QLQ. 1 hit.
PF14619. SnAC. 1 hit.
PF00176. SNF2_N. 1 hit.
[Graphical view]
PRINTSiPR00503. BROMODOMAIN.
SMARTiSM00384. AT_hook. 2 hits.
SM00297. BROMO. 1 hit.
SM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
SM00951. QLQ. 1 hit.
SM01314. SnAC. 1 hit.
[Graphical view]
SUPFAMiSSF47370. SSF47370. 1 hit.
SSF52540. SSF52540. 2 hits.
PROSITEiPS00633. BROMODOMAIN_1. 1 hit.
PS50014. BROMODOMAIN_2. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51204. HSA. 1 hit.
PS51666. QLQ. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSNF2_YEAST
AccessioniPrimary (citable) accession number: P22082
Secondary accession number(s): D6W2Y8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: August 1, 1991
Last modified: November 30, 2016
This is version 174 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 217 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.