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P22082 (SNF2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 150. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Transcription regulatory protein SNF2

EC=3.6.4.-
Alternative name(s):
ATP-dependent helicase SNF2
Regulatory protein GAM1
Regulatory protein SWI2
SWI/SNF complex component SNF2
Transcription factor TYE3
Gene names
Name:SNF2
Synonyms:GAM1, RIC1, SWI2, TYE3
Ordered Locus Names:YOR290C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length1703 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in transcriptional activation. Catalytic component of the SWI/SNF complex, an ATP-dependent chromatin-remodeling complex, which is required for the positive and negative regulation of gene expression of a large number of genes. It changes chromatin structure by altering DNA-histone contacts within a nucleosome, leading eventually to a change in nucleosome position, thus facilitating or repressing binding of gene-specific transcription factors.

Subunit structure

Component of the SWI/SNF global transcription activator complex. The 1.14 MDa SWI/SNF complex is composed of 11 different subunits: one copy each of SWI1, SNF2/SWI2, SNF5, SNF12/SWP73, ARP7/SWP61, ARP9/SWP59; two copies each of SWI3, SNF6, SNF11, SWP82; and three copies of TAF14/SWP29.

Subcellular location

Nucleus.

Miscellaneous

Present with 217 molecules/cell in log phase SD medium.

Sequence similarities

Contains 3 A.T hook DNA-binding domains.

Contains 1 bromo domain.

Contains 1 helicase ATP-binding domain.

Contains 1 helicase C-terminal domain.

Contains 1 HSA domain.

Contains 1 QLQ domain.

Ontologies

Keywords
   Biological processTranscription
Transcription regulation
   Cellular componentNucleus
   DomainBromodomain
Repeat
   LigandATP-binding
Nucleotide-binding
   Molecular functionActivator
Helicase
Hydrolase
   PTMPhosphoprotein
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processATP catabolic process

Inferred from direct assay PubMed 17188033PubMed 8127913PubMed 8458575. Source: GOC

ATP-dependent chromatin remodeling

Inferred from direct assay PubMed 8016655. Source: SGD

DNA-dependent DNA replication

Inferred from mutant phenotype PubMed 10198436. Source: SGD

cellular alcohol catabolic process

Inferred from mutant phenotype PubMed 1339306. Source: SGD

chromatin remodeling

Inferred from mutant phenotype PubMed 1459453. Source: SGD

double-strand break repair

Inferred from mutant phenotype PubMed 16024655. Source: SGD

nucleosome mobilization

Inferred from direct assay PubMed 17496903. Source: SGD

positive regulation of mating type switching

Inferred from mutant phenotype PubMed 6436497. Source: SGD

positive regulation of transcription from RNA polymerase II promoter

Inferred from mutant phenotype PubMed 1339306PubMed 17681272PubMed 3542227PubMed 8016655. Source: SGD

positive regulation of transcription from RNA polymerase II promoter in response to amino acid starvation

Inferred from mutant phenotype PubMed 10549298. Source: SGD

strand invasion

Inferred from mutant phenotype PubMed 19766565. Source: SGD

sucrose catabolic process

Inferred from mutant phenotype PubMed 1339306PubMed 6392017. Source: SGD

transcription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentSWI/SNF complex

Inferred from direct assay PubMed 18644858PubMed 8016655PubMed 8127913PubMed 8159677. Source: SGD

nucleus

Inferred from direct assay Ref.1PubMed 22932476. Source: SGD

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

DNA-dependent ATPase activity

Inferred from direct assay PubMed 8127913PubMed 8458575. Source: SGD

RNA polymerase II activating transcription factor binding

Inferred from physical interaction PubMed 11865042. Source: SGD

helicase activity

Inferred from electronic annotation. Source: UniProtKB-KW

lysine-acetylated histone binding

Inferred from direct assay PubMed 20126658. Source: SGD

nucleosomal DNA binding

Inferred from direct assay PubMed 18644858. Source: SGD

protein binding

Inferred from physical interaction PubMed 16429126PubMed 22997150PubMed 7623818PubMed 8016655PubMed 8127913PubMed 9726966. Source: IntAct

Complete GO annotation...

Binary interactions

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 17031703Transcription regulatory protein SNF2
PRO_0000074358

Regions

Domain247 – 28236QLQ
Domain588 – 66174HSA
Domain779 – 944166Helicase ATP-binding
Domain1091 – 1254164Helicase C-terminal
Domain1568 – 163871Bromo
Nucleotide binding792 – 7998ATP By similarity
DNA binding1446 – 145611A.T hook 1
DNA binding1502 – 151312A.T hook 2
DNA binding1516 – 152611A.T hook 3
Motif894 – 8974DEGH box
Compositional bias55 – 6814Gln-rich
Compositional bias207 – 23933Ala/Gln-rich

Amino acid modifications

Modified residue3581Phosphoserine Ref.10 Ref.11 Ref.12
Modified residue3831Phosphothreonine Ref.12
Modified residue7161Phosphoserine Ref.11 Ref.12
Modified residue13401Phosphoserine Ref.11

Secondary structure

... 1703
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P22082 [UniParc].

Last modified August 1, 1991. Version 1.
Checksum: 84B8BC595C8F3E6D

FASTA1,703194,051
        10         20         30         40         50         60 
MNIPQRQFSN EEVNRCYLRW QHLRNEHGMN APSVPEFIYL TKVLQFAAKQ RQELQMQRQQ 

        70         80         90        100        110        120 
QGISGSQQNI VPNSSDQAEL PNNASSHISA SASPHLAPNM QLNGNETFST SAHQSPIMQT 

       130        140        150        160        170        180 
QMPLNSNGGN NMLPQRQSSV GSLNATNFSP TPANNGENAA EKPDNSNHNN LNLNNSELQP 

       190        200        210        220        230        240 
QNRSLQEHNI QDSNVMPGSQ INSPMPQQAQ MQQAQFQAQQ AQQAQQAQQA QQAQARLQQG 

       250        260        270        280        290        300 
RRLPMTMFTA EQSELLKAQI TSLKCLVNRK PIPFEFQAVI QKSINHPPDF KRMLLSLSEF 

       310        320        330        340        350        360 
ARRRQPTDQN NQSNLNGGNN TQQPGTNSHY NNTNTDNVSG LTRNAPLDSK DENFASVSPA 

       370        380        390        400        410        420 
GPSSVHNAKN GTLDKNSQTV SGTPITQTES KKEENETISN VAKTAPNSNK THTEQNNPPK 

       430        440        450        460        470        480 
PQKPVPLNVL QDQYKEGIKV VDIDDPDMMV DSFTMPNISH SNIDYQTLLA NSDHAKFTIE 

       490        500        510        520        530        540 
PGVLPVGIDT HTATDIYQTL IALNLDTTVN DCLDKLLNDE CTESTRENAL YDYYALQLLP 

       550        560        570        580        590        600 
LQKAVRGHVL QFEWHQNSLL TNTHPNFLSK IRNINVQDAL LTNQLYKNHE LLKLERKKTE 

       610        620        630        640        650        660 
AVARLKSMNK SAINQYNRRQ DKKNKRLKFG HRLIATHTNL ERDEQKRAEK KAKERLQALK 

       670        680        690        700        710        720 
ANDEEAYIKL LDQTKDTRIT HLLRQTNAFL DSLTRAVKDQ QKYTKEMIDS HIKEASEEVD 

       730        740        750        760        770        780 
DLSMVPKMKD EEYDDDDDNS NVDYYNVAHR IKEDIKKQPS ILVGGTLKDY QIKGLQWMVS 

       790        800        810        820        830        840 
LFNNHLNGIL ADEMGLGKTI QTISLLTYLY EMKNIRGPYL VIVPLSTLSN WSSEFAKWAP 

       850        860        870        880        890        900 
TLRTISFKGS PNERKAKQAK IRAGEFDVVL TTFEYIIKER ALLSKVKWVH MIIDEGHRMK 

       910        920        930        940        950        960 
NAQSKLSLTL NTHYHADYRL ILTGTPLQNN LPELWALLNF VLPKIFNSVK SFDEWFNTPF 

       970        980        990       1000       1010       1020 
ANTGGQDKIE LSEEETLLVI RRLHKVLRPF LLRRLKKDVE KELPDKVEKV VKCKMSALQQ 

      1030       1040       1050       1060       1070       1080 
IMYQQMLKYR RLFIGDQNNK KMVGLRGFNN QIMQLKKICN HPFVFEEVED QINPTRETND 

      1090       1100       1110       1120       1130       1140 
DIWRVAGKFE LLDRILPKLK ATGHRVLIFF QMTQIMDIME DFLRYINIKY LRLDGHTKSD 

      1150       1160       1170       1180       1190       1200 
ERSELLRLFN APDSEYLCFI LSTRAGGLGL NLQTADTVII FDTDWNPHQD LQAQDRAHRI 

      1210       1220       1230       1240       1250       1260 
GQKNEVRILR LITTNSVEEV ILERAYKKLD IDGKVIQAGK FDNKSTSEEQ EALLRSLLDA 

      1270       1280       1290       1300       1310       1320 
EEERRKKRES GVEEEEELKD SEINEILARN DEEMAVLTRM DEDRSKKEEE LGVKSRLLEK 

      1330       1340       1350       1360       1370       1380 
SELPDIYSRD IGAELKREES ESAAVYNGRG ARERKTATYN DNMSEEQWLR QFEVSDDEKN 

      1390       1400       1410       1420       1430       1440 
DKQARKQRTK KEDKSEAIDG NGEIKGENID ADNDGPRINN ISAEDRADTD LAMNDDDFLS 

      1450       1460       1470       1480       1490       1500 
KKRKAGRPRG RPKKVKLEGS ENSEPPALES SPVTGDNSPS EDFMDIPKPR TAGKTSVKSA 

      1510       1520       1530       1540       1550       1560 
RTSTRGRGRG RGRGRGRGRG RGRPPKARNG LDYVRTPAAA TSPIDIREKV AKQALDLYHF 

      1570       1580       1590       1600       1610       1620 
ALNYENEAGR KLSDIFLSKP SKALYPDYYM IIKYPVAFDN INTHIETLAY NSLKETLQDF 

      1630       1640       1650       1660       1670       1680 
HLIFSNARIY NTEGSVVYED SLELEKVVTK KYCEIMGDNS QLDFTEFDEQ YGTRPLVLPP 

      1690       1700 
VVTSSVAESF TDEADSSMTE ASV 

« Hide

References

« Hide 'large scale' references
[1]"Functional interdependence of the yeast SNF2, SNF5, and SNF6 proteins in transcriptional activation."
Laurent B.C., Treitel M.A., Carlson M.
Proc. Natl. Acad. Sci. U.S.A. 88:2687-2691(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"The GAM1/SNF2 gene of Saccharomyces cerevisiae encodes a highly charged nuclear protein required for transcription of the STA1 gene."
Yoshimoto H., Yamashita I.
Mol. Gen. Genet. 228:270-280(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 38626 / AH22 / NRRL Y-12843.
[3]"The SNF2/SWI2/GAM1/TYE3/RIC1 gene is involved in the coordinate regulation of phospholipid synthesis in Saccharomyces cerevisiae."
Kodaki T., Hosaka K., Nikawa J., Yamashita S.
J. Biochem. 117:362-368(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 26787 / X2180-1B.
[4]"DNA sequence analysis of the VPH1-SNF2 region on chromosome XV of Saccharomyces cerevisiae."
Cheret G., Bernardi A., Sor F.J.
Yeast 12:1059-1064(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[5]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D. expand/collapse author list , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[6]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[7]"Sequence and analysis of a 36.2 kb fragment from the right arm of yeast chromosome XV reveals 19 open reading frames including SNF2 (5' end), CPA1, SLY41, a putative transport ATPase, a putative ribosomal protein and an SNF2 homologue."
Poirey R., Cziepluch C., Tobiasch E., Pujol A., Kordes E., Jauniaux J.-C.
Yeast 13:479-482(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-309.
Strain: ATCC 96604 / S288c / FY1679.
[8]"Functional analysis of the DNA-stimulated ATPase domain of yeast SWI2/SNF2."
Richmond E., Peterson C.L.
Nucleic Acids Res. 24:3685-3692(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: MUTAGENESIS.
[9]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[10]"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-358, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: ADR376.
[11]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-358; SER-716 AND SER-1340, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[12]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-358; THR-383 AND SER-716, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[13]"Sites of ubiquitin attachment in Saccharomyces cerevisiae."
Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[14]"Structural analysis of the yeast SWI/SNF chromatin remodeling complex."
Smith C.L., Horowitz-Scherer R., Flanagan J.F., Woodcock C.L., Peterson C.L.
Nat. Struct. Biol. 10:141-145(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: 3D-STRUCTURE MODELING OF THE SWI/SNF COMPLEX, ELECTRON MICROSCOPY OF THE SWI/SNF COMPLEX.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M61703 Genomic DNA. Translation: AAA35059.1.
X57837 Genomic DNA. Translation: CAA40969.1.
D90459 Genomic DNA. Translation: BAA14423.1.
X89633 Genomic DNA. Translation: CAA61793.1.
Z75198 Genomic DNA. Translation: CAA99517.1.
Z75199 Genomic DNA. Translation: CAA99519.1.
BK006948 Genomic DNA. Translation: DAA11054.1.
PIRS15047.
RefSeqNP_014933.3. NM_001183709.3.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
4I6MX-ray2.80C575-667[»]
ProteinModelPortalP22082.
SMRP22082. Positions 592-660, 753-1241, 1565-1655.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid34678. 142 interactions.
DIPDIP-1150N.
IntActP22082. 42 interactions.
MINTMINT-600461.
STRING4932.YOR290C.

Proteomic databases

MaxQBP22082.
PaxDbP22082.
PeptideAtlasP22082.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYOR290C; YOR290C; YOR290C.
GeneID854465.
KEGGsce:YOR290C.

Organism-specific databases

CYGDYOR290c.
SGDS000005816. SNF2.

Phylogenomic databases

eggNOGCOG0553.
GeneTreeENSGT00750000117971.
HOGENOMHOG000172362.
KOK11786.
OMAFVYEEVE.
OrthoDBEOG7M98QM.

Enzyme and pathway databases

BioCycYEAST:G3O-33775-MONOMER.

Gene expression databases

GenevestigatorP22082.

Family and domain databases

Gene3D1.20.920.10. 1 hit.
3.40.50.300. 2 hits.
InterProIPR017956. AT_hook_DNA-bd_motif.
IPR001487. Bromodomain.
IPR018359. Bromodomain_CS.
IPR014978. Gln-Leu-Gln_QLQ.
IPR014012. Helicase/SANT-assoc_DNA-bd.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR029295. SnAC.
IPR000330. SNF2_N.
[Graphical view]
PfamPF00439. Bromodomain. 1 hit.
PF00271. Helicase_C. 1 hit.
PF08880. QLQ. 1 hit.
PF14619. SnAC. 1 hit.
PF00176. SNF2_N. 1 hit.
[Graphical view]
PRINTSPR00503. BROMODOMAIN.
SMARTSM00384. AT_hook. 2 hits.
SM00297. BROMO. 1 hit.
SM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
SM00951. QLQ. 1 hit.
[Graphical view]
SUPFAMSSF47370. SSF47370. 1 hit.
SSF52540. SSF52540. 2 hits.
PROSITEPS00633. BROMODOMAIN_1. 1 hit.
PS50014. BROMODOMAIN_2. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51204. HSA. 1 hit.
PS51666. QLQ. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio976750.

Entry information

Entry nameSNF2_YEAST
AccessionPrimary (citable) accession number: P22082
Secondary accession number(s): D6W2Y8
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: August 1, 1991
Last modified: July 9, 2014
This is version 150 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XV

Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references