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P22079 (PERL_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 142. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Lactoperoxidase

Short name=LPO
EC=1.11.1.7
Alternative name(s):
Salivary peroxidase
Short name=SPO
Gene names
Name:LPO
Synonyms:SAPX
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length712 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

May contribute to airway host defense against infection. Ref.7

Catalytic activity

2 phenolic donor + H2O2 = 2 phenoxyl radical of the donor + 2 H2O.

Cofactor

Binds 1 calcium ion per heterodimer By similarity.

Binds 1 heme B (iron-protoporphyrin IX) group covalently per heterodimer By similarity.

Subcellular location

Secretedextracellular space.

Tissue specificity

Mammary gland; milk and salivary gland. Found in bronchial submucosal glands. Ref.7

Sequence similarities

Belongs to the peroxidase family. XPO subfamily.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Note: Additional isoforms seem to exist.
Isoform 1 (identifier: P22079-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: P22079-2)

Also known as: V3;

The sequence of this isoform differs from the canonical sequence as follows:
     26-108: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2626 Potential
Propeptide27 – 10074 By similarity
PRO_0000023649
Chain101 – 712612Lactoperoxidase
PRO_0000023650

Sites

Active site2261Proton acceptor By similarity
Metal binding2271Calcium By similarity
Metal binding3011Calcium By similarity
Metal binding3031Calcium; via carbonyl oxygen By similarity
Metal binding3051Calcium By similarity
Metal binding3071Calcium By similarity
Metal binding4681Iron (heme axial ligand) By similarity
Binding site2251Heme (covalent; via 2 links) By similarity
Binding site3751Heme (covalent; via 2 links) By similarity
Site3721Transition state stabilizer By similarity

Amino acid modifications

Modified residue3151Phosphoserine By similarity
Glycosylation1061N-linked (GlcNAc...) Potential
Glycosylation2121N-linked (GlcNAc...) Ref.8
Glycosylation3221N-linked (GlcNAc...) Potential
Glycosylation3581N-linked (GlcNAc...) Ref.8 Ref.9
Disulfide bond132 ↔ 145 By similarity
Disulfide bond246 ↔ 256 By similarity
Disulfide bond250 ↔ 274 By similarity
Disulfide bond354 ↔ 365 By similarity
Disulfide bond573 ↔ 630 By similarity
Disulfide bond671 ↔ 696 By similarity

Natural variations

Alternative sequence26 – 10883Missing in isoform 2.
VSP_044473
Natural variant1051T → I. Ref.3
Corresponds to variant rs8178318 [ dbSNP | Ensembl ].
VAR_018809
Natural variant2441A → T. Ref.3
Corresponds to variant rs8178338 [ dbSNP | Ensembl ].
VAR_018810
Natural variant4141R → Q. Ref.3
Corresponds to variant rs8178355 [ dbSNP | Ensembl ].
VAR_018811
Natural variant4211V → M. Ref.2 Ref.3 Ref.6
Corresponds to variant rs2301870 [ dbSNP | Ensembl ].
VAR_018812
Natural variant5141R → Q. Ref.3
Corresponds to variant rs8178401 [ dbSNP | Ensembl ].
VAR_018813
Natural variant6141I → T. Ref.3
Corresponds to variant rs8178408 [ dbSNP | Ensembl ].
VAR_018814
Natural variant7001D → N. Ref.3
Corresponds to variant rs8178412 [ dbSNP | Ensembl ].
VAR_018815

Experimental info

Sequence conflict2551N → S in ABQ53140. Ref.2
Sequence conflict6781K → M in ABQ53140. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified January 24, 2001. Version 2.
Checksum: BC7AA410997198C0

FASTA71280,288
        10         20         30         40         50         60 
MRVLLHLPAL LASLILLQAA ASTTRAQTTR TSAISDTVSQ AKVQVNKAFL DSRTRLKTAM 

        70         80         90        100        110        120 
SSETPTSRQL SEYLKHAKGR TRTAIRNGQV WEESLKRLRQ KASLTNVTDP SLDLTSLSLE 

       130        140        150        160        170        180 
VGCGAPAPVV RCDPCSPYRT ITGDCNNRRK PALGAANRAL ARWLPAEYED GLSLPFGWTP 

       190        200        210        220        230        240 
GKTRNGFPLP LAREVSNKIV GYLNEEGVLD QNRSLLFMQW GQIVDHDLDF APDTELGSSE 

       250        260        270        280        290        300 
YSKAQCDEYC IQGDNCFPIM FPPNDPKAGT QGKCMPFFRA GFVCPTPPYK SLAREQINAL 

       310        320        330        340        350        360 
TSFLDASFVY SSEPSLASRL RNLSSPLGLM AVNQEVSDHG LPYLPYDSKK PSPCEFINTT 

       370        380        390        400        410        420 
ARVPCFLAGD SRASEHILLA TSHTLFLREH NRLARELKRL NPQWDGEKLY QEARKILGAF 

       430        440        450        460        470        480 
VQIITFRDYL PILLGDHMQK WIPPYQGYSE SVDPRISNVF TFAFRFGHLE VPSSMFRLDE 

       490        500        510        520        530        540 
NYQPWGPEPE LPLHTLFFNT WRMVKDGGID PLVRGLLAKK SKLMKQNKMM TGELRNKLFQ 

       550        560        570        580        590        600 
PTHRIHGFDL AAINTQRCRD HGQPGYNSWR AFCDLSQPQT LEELNTVLKS KMLAKKLLGL 

       610        620        630        640        650        660 
YGTPDNIDIW IGAIAEPLVE RGRVGPLLAC LLGKQFQQIR DGDRFWWENP GVFTNEQKDS 

       670        680        690        700        710 
LQKMSFSRLV CDNTRITKVP RDPFWANSYP YDFVDCSAID KLDLSPWASV KN 

« Hide

Isoform 2 (V3) [UniParc].

Checksum: 8A8BBBB5E48C8BBD
Show »

FASTA62970,984

References

« Hide 'large scale' references
[1]"Cloning and sequence analysis of the human salivary peroxidase-encoding cDNA."
Kiser C., Caterina J., Engler J.A., Rahemtulla B., Rahemtulla F.
Gene 173:261-264(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Tissue: Submandibular gland.
[2]"Molecular heterogeneity and alternative splicing of human lactoperoxidase."
Fragoso M.A., Torbati A., Fregien N., Conner G.E.
Arch. Biochem. Biophys. 482:52-57(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT MET-421, ALTERNATIVE SPLICING.
[3]NIEHS SNPs program
Submitted (JUN-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS ILE-105; THR-244; GLN-414; MET-421; GLN-514; THR-614 AND ASN-700.
[4]"DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage."
Zody M.C., Garber M., Adams D.J., Sharpe T., Harrow J., Lupski J.R., Nicholson C., Searle S.M., Wilming L., Young S.K., Abouelleil A., Allen N.R., Bi W., Bloom T., Borowsky M.L., Bugalter B.E., Butler J., Chang J.L. expand/collapse author list , Chen C.-K., Cook A., Corum B., Cuomo C.A., de Jong P.J., DeCaprio D., Dewar K., FitzGerald M., Gilbert J., Gibson R., Gnerre S., Goldstein S., Grafham D.V., Grocock R., Hafez N., Hagopian D.S., Hart E., Norman C.H., Humphray S., Jaffe D.B., Jones M., Kamal M., Khodiyar V.K., LaButti K., Laird G., Lehoczky J., Liu X., Lokyitsang T., Loveland J., Lui A., Macdonald P., Major J.E., Matthews L., Mauceli E., McCarroll S.A., Mihalev A.H., Mudge J., Nguyen C., Nicol R., O'Leary S.B., Osoegawa K., Schwartz D.C., Shaw-Smith C., Stankiewicz P., Steward C., Swarbreck D., Venkataraman V., Whittaker C.A., Yang X., Zimmer A.R., Bradley A., Hubbard T., Birren B.W., Rogers J., Lander E.S., Nusbaum C.
Nature 440:1045-1049(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[6]"Molecular cloning of cDNAs encoding bovine and human lactoperoxidase."
Dull T.J., Uyeda C., Strosberg A.D., Nedwin G., Seilhamer J.J.
DNA Cell Biol. 9:499-509(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 389-712, VARIANT MET-421.
[7]"Lactoperoxidase and human airway host defense."
Wijkstrom-Frei C., El-Chemaly S., Ali-Rachedi R., Gerson C., Cobas M.A., Forteza R., Salathe M., Conner G.E.
Am. J. Respir. Cell Mol. Biol. 29:206-212(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, TISSUE SPECIFICITY.
[8]"Identification of N-linked glycoproteins in human saliva by glycoprotein capture and mass spectrometry."
Ramachandran P., Boontheung P., Xie Y., Sondej M., Wong D.T., Loo J.A.
J. Proteome Res. 5:1493-1503(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-212 AND ASN-358.
Tissue: Saliva.
[9]"Identification of N-linked glycoproteins in human milk by hydrophilic interaction liquid chromatography and mass spectrometry."
Picariello G., Ferranti P., Mamone G., Roepstorff P., Addeo F.
Proteomics 8:3833-3847(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-358.
Tissue: Milk.
+Additional computationally mapped references.

Web resources

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U39573 mRNA. Translation: AAC50717.1.
EF579964 mRNA. Translation: ABQ53140.1.
AY324876 Genomic DNA. Translation: AAP72968.1.
AC004687 Genomic DNA. No translation available.
AC005962 Genomic DNA. No translation available.
BC107166 mRNA. Translation: AAI07167.1.
BC107167 mRNA. Translation: AAI07168.1.
M58151 mRNA. Translation: AAA63213.1.
PIRJC4935.
RefSeqNP_001153574.1. NM_001160102.1.
NP_006142.1. NM_006151.2.
UniGeneHs.234742.

3D structure databases

ProteinModelPortalP22079.
SMRP22079. Positions 130-712.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid110207. 1 interaction.
STRING9606.ENSP00000262290.

Chemistry

BindingDBP22079.
ChEMBLCHEMBL5898.

Protein family/group databases

PeroxiBase3316. HsLPO.

PTM databases

PhosphoSiteP22079.

Polymorphism databases

DMDM12643419.

Proteomic databases

PaxDbP22079.
PeptideAtlasP22079.
PRIDEP22079.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000262290; ENSP00000262290; ENSG00000167419. [P22079-1]
ENST00000421678; ENSP00000400245; ENSG00000167419. [P22079-2]
ENST00000582328; ENSP00000464636; ENSG00000167419. [P22079-2]
GeneID4025.
KEGGhsa:4025.
UCSCuc002ivt.3. human. [P22079-1]
uc010dcp.3. human. [P22079-2]

Organism-specific databases

CTD4025.
GeneCardsGC17P056315.
HGNCHGNC:6678. LPO.
HPAHPA028688.
MIM150205. gene.
170990. gene.
neXtProtNX_P22079.
PharmGKBPA30439.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG262194.
HOGENOMHOG000016084.
HOVERGENHBG000071.
InParanoidP22079.
KOK12550.
OMASPWASVK.
OrthoDBEOG7M0NQW.
PhylomeDBP22079.
TreeFamTF314316.

Gene expression databases

ArrayExpressP22079.
BgeeP22079.
CleanExHS_LPO.
GenevestigatorP22079.

Family and domain databases

Gene3D1.10.640.10. 2 hits.
InterProIPR010255. Haem_peroxidase.
IPR002007. Haem_peroxidase_animal.
IPR019791. Haem_peroxidase_animal_subgr.
[Graphical view]
PfamPF03098. An_peroxidase. 1 hit.
[Graphical view]
PRINTSPR00457. ANPEROXIDASE.
SUPFAMSSF48113. SSF48113. 1 hit.
PROSITEPS50292. PEROXIDASE_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSLPO. human.
GeneWikiLactoperoxidase.
GenomeRNAi4025.
NextBio15782.
PROP22079.
SOURCESearch...

Entry information

Entry namePERL_HUMAN
AccessionPrimary (citable) accession number: P22079
Secondary accession number(s): A5JUY4 expand/collapse secondary AC list , E7EMJ3, Q13408, Q3KNQ2
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: January 24, 2001
Last modified: April 16, 2014
This is version 142 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 17

Human chromosome 17: entries, gene names and cross-references to MIM