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P22079

- PERL_HUMAN

UniProt

P22079 - PERL_HUMAN

Protein

Lactoperoxidase

Gene

LPO

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 146 (01 Oct 2014)
      Sequence version 2 (24 Jan 2001)
      Previous versions | rss
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    Functioni

    May contribute to airway host defense against infection.1 Publication

    Catalytic activityi

    2 phenolic donor + H2O2 = 2 phenoxyl radical of the donor + 2 H2O.

    Cofactori

    Binds 1 calcium ion per heterodimer.PROSITE-ProRule annotation
    Binds 1 heme B (iron-protoporphyrin IX) group covalently per heterodimer.PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei225 – 2251Heme (covalent; via 2 links)By similarity
    Active sitei226 – 2261Proton acceptorPROSITE-ProRule annotation
    Metal bindingi227 – 2271CalciumPROSITE-ProRule annotation
    Metal bindingi301 – 3011CalciumPROSITE-ProRule annotation
    Metal bindingi303 – 3031Calcium; via carbonyl oxygenPROSITE-ProRule annotation
    Metal bindingi305 – 3051CalciumPROSITE-ProRule annotation
    Metal bindingi307 – 3071CalciumPROSITE-ProRule annotation
    Sitei372 – 3721Transition state stabilizerPROSITE-ProRule annotation
    Binding sitei375 – 3751Heme (covalent; via 2 links)By similarity
    Metal bindingi468 – 4681Iron (heme axial ligand)PROSITE-ProRule annotation

    GO - Molecular functioni

    1. heme binding Source: InterPro
    2. metal ion binding Source: UniProtKB-KW
    3. thiocyanate peroxidase activity Source: UniProt

    GO - Biological processi

    1. defense response to bacterium Source: UniProt
    2. detection of chemical stimulus involved in sensory perception of bitter taste Source: UniProt
    3. hydrogen peroxide catabolic process Source: UniProtKB-KW
    4. response to oxidative stress Source: UniProtKB
    5. thiocyanate metabolic process Source: GOC

    Keywords - Molecular functioni

    Oxidoreductase, Peroxidase

    Keywords - Biological processi

    Hydrogen peroxide

    Keywords - Ligandi

    Calcium, Heme, Iron, Metal-binding

    Protein family/group databases

    PeroxiBasei3316. HsLPO.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Lactoperoxidase (EC:1.11.1.7)
    Short name:
    LPO
    Alternative name(s):
    Salivary peroxidase
    Short name:
    SPO
    Gene namesi
    Name:LPO
    Synonyms:SAPX
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 17

    Organism-specific databases

    HGNCiHGNC:6678. LPO.

    Subcellular locationi

    GO - Cellular componenti

    1. basolateral plasma membrane Source: UniProt
    2. cytoplasm Source: Ensembl
    3. extracellular space Source: UniProt
    4. extracellular vesicular exosome Source: UniProt

    Keywords - Cellular componenti

    Secreted

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA30439.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2626Sequence AnalysisAdd
    BLAST
    Propeptidei27 – 10074By similarityPRO_0000023649Add
    BLAST
    Chaini101 – 712612LactoperoxidasePRO_0000023650Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi106 – 1061N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi132 ↔ 145PROSITE-ProRule annotation
    Glycosylationi212 – 2121N-linked (GlcNAc...)1 Publication
    Disulfide bondi246 ↔ 256PROSITE-ProRule annotation
    Disulfide bondi250 ↔ 274PROSITE-ProRule annotation
    Modified residuei315 – 3151PhosphoserineBy similarity
    Glycosylationi322 – 3221N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi354 ↔ 365PROSITE-ProRule annotation
    Glycosylationi358 – 3581N-linked (GlcNAc...)2 Publications
    Disulfide bondi573 ↔ 630PROSITE-ProRule annotation
    Disulfide bondi671 ↔ 696PROSITE-ProRule annotation

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Phosphoprotein

    Proteomic databases

    PaxDbiP22079.
    PeptideAtlasiP22079.
    PRIDEiP22079.

    PTM databases

    PhosphoSiteiP22079.

    Expressioni

    Tissue specificityi

    Mammary gland; milk and salivary gland. Found in bronchial submucosal glands.1 Publication

    Gene expression databases

    ArrayExpressiP22079.
    BgeeiP22079.
    CleanExiHS_LPO.
    GenevestigatoriP22079.

    Organism-specific databases

    HPAiHPA028688.

    Interactioni

    Protein-protein interaction databases

    BioGridi110207. 1 interaction.
    STRINGi9606.ENSP00000262290.

    Structurei

    3D structure databases

    ProteinModelPortaliP22079.
    SMRiP22079. Positions 130-712.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the peroxidase family. XPO subfamily.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG262194.
    HOGENOMiHOG000016084.
    HOVERGENiHBG000071.
    InParanoidiP22079.
    KOiK12550.
    OMAiSPWASVK.
    OrthoDBiEOG7M0NQW.
    PhylomeDBiP22079.
    TreeFamiTF314316.

    Family and domain databases

    Gene3Di1.10.640.10. 2 hits.
    InterProiIPR010255. Haem_peroxidase.
    IPR019791. Haem_peroxidase_animal.
    IPR029587. LPO.
    [Graphical view]
    PANTHERiPTHR11475:SF53. PTHR11475:SF53. 1 hit.
    PfamiPF03098. An_peroxidase. 1 hit.
    [Graphical view]
    PRINTSiPR00457. ANPEROXIDASE.
    SUPFAMiSSF48113. SSF48113. 1 hit.
    PROSITEiPS50292. PEROXIDASE_3. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Note: Additional isoforms seem to exist.

    Isoform 1 (identifier: P22079-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MRVLLHLPAL LASLILLQAA ASTTRAQTTR TSAISDTVSQ AKVQVNKAFL    50
    DSRTRLKTAM SSETPTSRQL SEYLKHAKGR TRTAIRNGQV WEESLKRLRQ 100
    KASLTNVTDP SLDLTSLSLE VGCGAPAPVV RCDPCSPYRT ITGDCNNRRK 150
    PALGAANRAL ARWLPAEYED GLSLPFGWTP GKTRNGFPLP LAREVSNKIV 200
    GYLNEEGVLD QNRSLLFMQW GQIVDHDLDF APDTELGSSE YSKAQCDEYC 250
    IQGDNCFPIM FPPNDPKAGT QGKCMPFFRA GFVCPTPPYK SLAREQINAL 300
    TSFLDASFVY SSEPSLASRL RNLSSPLGLM AVNQEVSDHG LPYLPYDSKK 350
    PSPCEFINTT ARVPCFLAGD SRASEHILLA TSHTLFLREH NRLARELKRL 400
    NPQWDGEKLY QEARKILGAF VQIITFRDYL PILLGDHMQK WIPPYQGYSE 450
    SVDPRISNVF TFAFRFGHLE VPSSMFRLDE NYQPWGPEPE LPLHTLFFNT 500
    WRMVKDGGID PLVRGLLAKK SKLMKQNKMM TGELRNKLFQ PTHRIHGFDL 550
    AAINTQRCRD HGQPGYNSWR AFCDLSQPQT LEELNTVLKS KMLAKKLLGL 600
    YGTPDNIDIW IGAIAEPLVE RGRVGPLLAC LLGKQFQQIR DGDRFWWENP 650
    GVFTNEQKDS LQKMSFSRLV CDNTRITKVP RDPFWANSYP YDFVDCSAID 700
    KLDLSPWASV KN 712
    Length:712
    Mass (Da):80,288
    Last modified:January 24, 2001 - v2
    Checksum:iBC7AA410997198C0
    GO
    Isoform 2 (identifier: P22079-2) [UniParc]FASTAAdd to Basket

    Also known as: V3

    The sequence of this isoform differs from the canonical sequence as follows:
         26-108: Missing.

    Show »
    Length:629
    Mass (Da):70,984
    Checksum:i8A8BBBB5E48C8BBD
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti255 – 2551N → S in ABQ53140. (PubMed:19059195)Curated
    Sequence conflicti678 – 6781K → M in ABQ53140. (PubMed:19059195)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti105 – 1051T → I.1 Publication
    Corresponds to variant rs8178318 [ dbSNP | Ensembl ].
    VAR_018809
    Natural varianti244 – 2441A → T.1 Publication
    Corresponds to variant rs8178338 [ dbSNP | Ensembl ].
    VAR_018810
    Natural varianti414 – 4141R → Q.1 Publication
    Corresponds to variant rs8178355 [ dbSNP | Ensembl ].
    VAR_018811
    Natural varianti421 – 4211V → M.3 Publications
    Corresponds to variant rs2301870 [ dbSNP | Ensembl ].
    VAR_018812
    Natural varianti514 – 5141R → Q.1 Publication
    Corresponds to variant rs8178401 [ dbSNP | Ensembl ].
    VAR_018813
    Natural varianti614 – 6141I → T.1 Publication
    Corresponds to variant rs8178408 [ dbSNP | Ensembl ].
    VAR_018814
    Natural varianti700 – 7001D → N.1 Publication
    Corresponds to variant rs8178412 [ dbSNP | Ensembl ].
    VAR_018815

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei26 – 10883Missing in isoform 2. CuratedVSP_044473Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U39573 mRNA. Translation: AAC50717.1.
    EF579964 mRNA. Translation: ABQ53140.1.
    AY324876 Genomic DNA. Translation: AAP72968.1.
    AC004687 Genomic DNA. No translation available.
    AC005962 Genomic DNA. No translation available.
    BC107166 mRNA. Translation: AAI07167.1.
    BC107167 mRNA. Translation: AAI07168.1.
    M58151 mRNA. Translation: AAA63213.1.
    CCDSiCCDS32689.1. [P22079-1]
    CCDS54149.1. [P22079-2]
    PIRiJC4935.
    RefSeqiNP_001153574.1. NM_001160102.1. [P22079-2]
    NP_006142.1. NM_006151.2. [P22079-1]
    UniGeneiHs.234742.

    Genome annotation databases

    EnsembliENST00000262290; ENSP00000262290; ENSG00000167419. [P22079-1]
    ENST00000421678; ENSP00000400245; ENSG00000167419. [P22079-2]
    ENST00000582328; ENSP00000464636; ENSG00000167419. [P22079-2]
    GeneIDi4025.
    KEGGihsa:4025.
    UCSCiuc002ivt.3. human. [P22079-1]
    uc010dcp.3. human. [P22079-2]

    Polymorphism databases

    DMDMi12643419.

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Cross-referencesi

    Web resourcesi

    NIEHS-SNPs

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U39573 mRNA. Translation: AAC50717.1 .
    EF579964 mRNA. Translation: ABQ53140.1 .
    AY324876 Genomic DNA. Translation: AAP72968.1 .
    AC004687 Genomic DNA. No translation available.
    AC005962 Genomic DNA. No translation available.
    BC107166 mRNA. Translation: AAI07167.1 .
    BC107167 mRNA. Translation: AAI07168.1 .
    M58151 mRNA. Translation: AAA63213.1 .
    CCDSi CCDS32689.1. [P22079-1 ]
    CCDS54149.1. [P22079-2 ]
    PIRi JC4935.
    RefSeqi NP_001153574.1. NM_001160102.1. [P22079-2 ]
    NP_006142.1. NM_006151.2. [P22079-1 ]
    UniGenei Hs.234742.

    3D structure databases

    ProteinModelPortali P22079.
    SMRi P22079. Positions 130-712.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 110207. 1 interaction.
    STRINGi 9606.ENSP00000262290.

    Chemistry

    BindingDBi P22079.
    ChEMBLi CHEMBL5898.

    Protein family/group databases

    PeroxiBasei 3316. HsLPO.

    PTM databases

    PhosphoSitei P22079.

    Polymorphism databases

    DMDMi 12643419.

    Proteomic databases

    PaxDbi P22079.
    PeptideAtlasi P22079.
    PRIDEi P22079.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000262290 ; ENSP00000262290 ; ENSG00000167419 . [P22079-1 ]
    ENST00000421678 ; ENSP00000400245 ; ENSG00000167419 . [P22079-2 ]
    ENST00000582328 ; ENSP00000464636 ; ENSG00000167419 . [P22079-2 ]
    GeneIDi 4025.
    KEGGi hsa:4025.
    UCSCi uc002ivt.3. human. [P22079-1 ]
    uc010dcp.3. human. [P22079-2 ]

    Organism-specific databases

    CTDi 4025.
    GeneCardsi GC17P056315.
    HGNCi HGNC:6678. LPO.
    HPAi HPA028688.
    MIMi 150205. gene.
    170990. gene.
    neXtProti NX_P22079.
    PharmGKBi PA30439.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG262194.
    HOGENOMi HOG000016084.
    HOVERGENi HBG000071.
    InParanoidi P22079.
    KOi K12550.
    OMAi SPWASVK.
    OrthoDBi EOG7M0NQW.
    PhylomeDBi P22079.
    TreeFami TF314316.

    Miscellaneous databases

    ChiTaRSi LPO. human.
    GeneWikii Lactoperoxidase.
    GenomeRNAii 4025.
    NextBioi 15782.
    PROi P22079.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P22079.
    Bgeei P22079.
    CleanExi HS_LPO.
    Genevestigatori P22079.

    Family and domain databases

    Gene3Di 1.10.640.10. 2 hits.
    InterProi IPR010255. Haem_peroxidase.
    IPR019791. Haem_peroxidase_animal.
    IPR029587. LPO.
    [Graphical view ]
    PANTHERi PTHR11475:SF53. PTHR11475:SF53. 1 hit.
    Pfami PF03098. An_peroxidase. 1 hit.
    [Graphical view ]
    PRINTSi PR00457. ANPEROXIDASE.
    SUPFAMi SSF48113. SSF48113. 1 hit.
    PROSITEi PS50292. PEROXIDASE_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and sequence analysis of the human salivary peroxidase-encoding cDNA."
      Kiser C., Caterina J., Engler J.A., Rahemtulla B., Rahemtulla F.
      Gene 173:261-264(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
      Tissue: Submandibular gland.
    2. "Molecular heterogeneity and alternative splicing of human lactoperoxidase."
      Fragoso M.A., Torbati A., Fregien N., Conner G.E.
      Arch. Biochem. Biophys. 482:52-57(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT MET-421, ALTERNATIVE SPLICING.
    3. NIEHS SNPs program
      Submitted (JUN-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS ILE-105; THR-244; GLN-414; MET-421; GLN-514; THR-614 AND ASN-700.
    4. "DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage."
      Zody M.C., Garber M., Adams D.J., Sharpe T., Harrow J., Lupski J.R., Nicholson C., Searle S.M., Wilming L., Young S.K., Abouelleil A., Allen N.R., Bi W., Bloom T., Borowsky M.L., Bugalter B.E., Butler J., Chang J.L.
      , Chen C.-K., Cook A., Corum B., Cuomo C.A., de Jong P.J., DeCaprio D., Dewar K., FitzGerald M., Gilbert J., Gibson R., Gnerre S., Goldstein S., Grafham D.V., Grocock R., Hafez N., Hagopian D.S., Hart E., Norman C.H., Humphray S., Jaffe D.B., Jones M., Kamal M., Khodiyar V.K., LaButti K., Laird G., Lehoczky J., Liu X., Lokyitsang T., Loveland J., Lui A., Macdonald P., Major J.E., Matthews L., Mauceli E., McCarroll S.A., Mihalev A.H., Mudge J., Nguyen C., Nicol R., O'Leary S.B., Osoegawa K., Schwartz D.C., Shaw-Smith C., Stankiewicz P., Steward C., Swarbreck D., Venkataraman V., Whittaker C.A., Yang X., Zimmer A.R., Bradley A., Hubbard T., Birren B.W., Rogers J., Lander E.S., Nusbaum C.
      Nature 440:1045-1049(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    6. "Molecular cloning of cDNAs encoding bovine and human lactoperoxidase."
      Dull T.J., Uyeda C., Strosberg A.D., Nedwin G., Seilhamer J.J.
      DNA Cell Biol. 9:499-509(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 389-712, VARIANT MET-421.
    7. Cited for: FUNCTION, TISSUE SPECIFICITY.
    8. "Identification of N-linked glycoproteins in human saliva by glycoprotein capture and mass spectrometry."
      Ramachandran P., Boontheung P., Xie Y., Sondej M., Wong D.T., Loo J.A.
      J. Proteome Res. 5:1493-1503(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-212 AND ASN-358.
      Tissue: Saliva.
    9. "Identification of N-linked glycoproteins in human milk by hydrophilic interaction liquid chromatography and mass spectrometry."
      Picariello G., Ferranti P., Mamone G., Roepstorff P., Addeo F.
      Proteomics 8:3833-3847(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-358.
      Tissue: Milk.

    Entry informationi

    Entry nameiPERL_HUMAN
    AccessioniPrimary (citable) accession number: P22079
    Secondary accession number(s): A5JUY4
    , E7EMJ3, Q13408, Q3KNQ2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1991
    Last sequence update: January 24, 2001
    Last modified: October 1, 2014
    This is version 146 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 17
      Human chromosome 17: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3