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Protein

Lactoperoxidase

Gene

LPO

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Antimicrobial agent which utilizes hydrogen peroxide and thiocyanate (SCN) to generate the antimicrobial substance hypothiocyanous acid (HOSCN) (By similarity). May contribute to airway host defense against infection.By similarity1 Publication

Catalytic activityi

2 phenolic donor + H2O2 = 2 phenoxyl radical of the donor + 2 H2O.

Cofactori

Protein has several cofactor binding sites:
  • Ca2+PROSITE-ProRule annotationNote: Binds 1 Ca2+ ion per heterodimer.PROSITE-ProRule annotation
  • heme bPROSITE-ProRule annotationNote: Binds 1 heme b (iron(II)-protoporphyrin IX) group covalently per heterodimer.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei225Heme (covalent; via 2 links)By similarity1
Active sitei226Proton acceptorPROSITE-ProRule annotation1
Metal bindingi227CalciumPROSITE-ProRule annotation1
Metal bindingi301CalciumPROSITE-ProRule annotation1
Metal bindingi303Calcium; via carbonyl oxygenPROSITE-ProRule annotation1
Metal bindingi305CalciumPROSITE-ProRule annotation1
Metal bindingi307CalciumPROSITE-ProRule annotation1
Sitei372Transition state stabilizerPROSITE-ProRule annotation1
Binding sitei375Heme (covalent; via 2 links)By similarity1
Metal bindingi468Iron (heme axial ligand)PROSITE-ProRule annotation1

GO - Molecular functioni

  • heme binding Source: InterPro
  • metal ion binding Source: UniProtKB-KW
  • thiocyanate peroxidase activity Source: UniProtKB

GO - Biological processi

  • defense response to bacterium Source: UniProtKB
  • detection of chemical stimulus involved in sensory perception of bitter taste Source: UniProtKB
  • hydrogen peroxide catabolic process Source: UniProtKB-KW
  • response to oxidative stress Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Antimicrobial, Oxidoreductase, Peroxidase

Keywords - Biological processi

Hydrogen peroxide

Keywords - Ligandi

Calcium, Heme, Iron, Metal-binding

Enzyme and pathway databases

BioCyciZFISH:HS09558-MONOMER.

Protein family/group databases

PeroxiBasei3316. HsLPO.

Names & Taxonomyi

Protein namesi
Recommended name:
Lactoperoxidase (EC:1.11.1.7)
Short name:
LPO
Alternative name(s):
Salivary peroxidase
Short name:
SPO
Gene namesi
Name:LPO
Synonyms:SAPX
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 17

Organism-specific databases

HGNCiHGNC:6678. LPO.

Subcellular locationi

GO - Cellular componenti

  • basolateral plasma membrane Source: UniProtKB
  • cytoplasm Source: GO_Central
  • extracellular exosome Source: UniProtKB
  • extracellular space Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Organism-specific databases

DisGeNETi4025.
OpenTargetsiENSG00000167419.
PharmGKBiPA30439.

Chemistry databases

ChEMBLiCHEMBL5898.

Polymorphism and mutation databases

BioMutaiLPO.
DMDMi12643419.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 26Sequence analysisAdd BLAST26
PropeptideiPRO_000002364927 – 801 PublicationAdd BLAST54
ChainiPRO_000002365081 – 712Lactoperoxidase1 PublicationAdd BLAST632

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi106N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi132 ↔ 145PROSITE-ProRule annotation
Glycosylationi212N-linked (GlcNAc...)1 Publication1
Disulfide bondi246 ↔ 256PROSITE-ProRule annotation
Disulfide bondi250 ↔ 274PROSITE-ProRule annotation
Modified residuei315PhosphoserineBy similarity1
Glycosylationi322N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi354 ↔ 365PROSITE-ProRule annotation
Glycosylationi358N-linked (GlcNAc...)2 Publications1
Disulfide bondi573 ↔ 630PROSITE-ProRule annotation
Disulfide bondi671 ↔ 696PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiP22079.
PeptideAtlasiP22079.
PRIDEiP22079.

PTM databases

iPTMnetiP22079.
PhosphoSitePlusiP22079.

Expressioni

Tissue specificityi

Mammary gland; milk and salivary gland. Found in bronchial submucosal glands.1 Publication

Gene expression databases

BgeeiENSG00000167419.
CleanExiHS_LPO.
ExpressionAtlasiP22079. baseline and differential.
GenevisibleiP22079. HS.

Organism-specific databases

HPAiHPA028688.

Interactioni

Protein-protein interaction databases

BioGridi110207. 1 interactor.
STRINGi9606.ENSP00000262290.

Chemistry databases

BindingDBiP22079.

Structurei

3D structure databases

ProteinModelPortaliP22079.
SMRiP22079.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peroxidase family. XPO subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG2408. Eukaryota.
ENOG410XPZ3. LUCA.
GeneTreeiENSGT00550000074325.
HOGENOMiHOG000016084.
HOVERGENiHBG000071.
InParanoidiP22079.
KOiK12550.
OMAiSPWASVK.
OrthoDBiEOG091G0236.
PhylomeDBiP22079.
TreeFamiTF314316.

Family and domain databases

Gene3Di1.10.640.10. 2 hits.
InterProiIPR010255. Haem_peroxidase.
IPR019791. Haem_peroxidase_animal.
IPR029587. LPO.
[Graphical view]
PANTHERiPTHR11475:SF67. PTHR11475:SF67. 1 hit.
PfamiPF03098. An_peroxidase. 1 hit.
[Graphical view]
PRINTSiPR00457. ANPEROXIDASE.
SUPFAMiSSF48113. SSF48113. 1 hit.
PROSITEiPS50292. PEROXIDASE_3. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Note: Additional isoforms seem to exist.
Isoform 1 (identifier: P22079-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRVLLHLPAL LASLILLQAA ASTTRAQTTR TSAISDTVSQ AKVQVNKAFL
60 70 80 90 100
DSRTRLKTAM SSETPTSRQL SEYLKHAKGR TRTAIRNGQV WEESLKRLRQ
110 120 130 140 150
KASLTNVTDP SLDLTSLSLE VGCGAPAPVV RCDPCSPYRT ITGDCNNRRK
160 170 180 190 200
PALGAANRAL ARWLPAEYED GLSLPFGWTP GKTRNGFPLP LAREVSNKIV
210 220 230 240 250
GYLNEEGVLD QNRSLLFMQW GQIVDHDLDF APDTELGSSE YSKAQCDEYC
260 270 280 290 300
IQGDNCFPIM FPPNDPKAGT QGKCMPFFRA GFVCPTPPYK SLAREQINAL
310 320 330 340 350
TSFLDASFVY SSEPSLASRL RNLSSPLGLM AVNQEVSDHG LPYLPYDSKK
360 370 380 390 400
PSPCEFINTT ARVPCFLAGD SRASEHILLA TSHTLFLREH NRLARELKRL
410 420 430 440 450
NPQWDGEKLY QEARKILGAF VQIITFRDYL PILLGDHMQK WIPPYQGYSE
460 470 480 490 500
SVDPRISNVF TFAFRFGHLE VPSSMFRLDE NYQPWGPEPE LPLHTLFFNT
510 520 530 540 550
WRMVKDGGID PLVRGLLAKK SKLMKQNKMM TGELRNKLFQ PTHRIHGFDL
560 570 580 590 600
AAINTQRCRD HGQPGYNSWR AFCDLSQPQT LEELNTVLKS KMLAKKLLGL
610 620 630 640 650
YGTPDNIDIW IGAIAEPLVE RGRVGPLLAC LLGKQFQQIR DGDRFWWENP
660 670 680 690 700
GVFTNEQKDS LQKMSFSRLV CDNTRITKVP RDPFWANSYP YDFVDCSAID
710
KLDLSPWASV KN
Length:712
Mass (Da):80,288
Last modified:January 24, 2001 - v2
Checksum:iBC7AA410997198C0
GO
Isoform 2 (identifier: P22079-2) [UniParc]FASTAAdd to basket
Also known as: V3

The sequence of this isoform differs from the canonical sequence as follows:
     26-108: Missing.

Show »
Length:629
Mass (Da):70,984
Checksum:i8A8BBBB5E48C8BBD
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti255N → S in ABQ53140 (PubMed:19059195).Curated1
Sequence conflicti678K → M in ABQ53140 (PubMed:19059195).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_018809105T → I.1 PublicationCorresponds to variant rs8178318dbSNPEnsembl.1
Natural variantiVAR_018810244A → T.1 PublicationCorresponds to variant rs8178338dbSNPEnsembl.1
Natural variantiVAR_018811414R → Q.1 PublicationCorresponds to variant rs8178355dbSNPEnsembl.1
Natural variantiVAR_018812421V → M.3 PublicationsCorresponds to variant rs2301870dbSNPEnsembl.1
Natural variantiVAR_018813514R → Q.1 PublicationCorresponds to variant rs8178401dbSNPEnsembl.1
Natural variantiVAR_018814614I → T.1 PublicationCorresponds to variant rs8178408dbSNPEnsembl.1
Natural variantiVAR_018815700D → N.1 PublicationCorresponds to variant rs8178412dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_04447326 – 108Missing in isoform 2. CuratedAdd BLAST83

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U39573 mRNA. Translation: AAC50717.1.
EF579964 mRNA. Translation: ABQ53140.1.
AY324876 Genomic DNA. Translation: AAP72968.1.
AC004687 Genomic DNA. No translation available.
AC005962 Genomic DNA. No translation available.
BC107166 mRNA. Translation: AAI07167.1.
BC107167 mRNA. Translation: AAI07168.1.
M58151 mRNA. Translation: AAA63213.1.
CCDSiCCDS32689.1. [P22079-1]
CCDS54149.1. [P22079-2]
PIRiJC4935.
RefSeqiNP_001153574.1. NM_001160102.1. [P22079-2]
NP_006142.1. NM_006151.2. [P22079-1]
UniGeneiHs.234742.

Genome annotation databases

EnsembliENST00000262290; ENSP00000262290; ENSG00000167419. [P22079-1]
ENST00000421678; ENSP00000400245; ENSG00000167419. [P22079-2]
ENST00000582328; ENSP00000464636; ENSG00000167419. [P22079-2]
GeneIDi4025.
KEGGihsa:4025.
UCSCiuc002ivt.3. human. [P22079-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Web resourcesi

NIEHS-SNPs

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U39573 mRNA. Translation: AAC50717.1.
EF579964 mRNA. Translation: ABQ53140.1.
AY324876 Genomic DNA. Translation: AAP72968.1.
AC004687 Genomic DNA. No translation available.
AC005962 Genomic DNA. No translation available.
BC107166 mRNA. Translation: AAI07167.1.
BC107167 mRNA. Translation: AAI07168.1.
M58151 mRNA. Translation: AAA63213.1.
CCDSiCCDS32689.1. [P22079-1]
CCDS54149.1. [P22079-2]
PIRiJC4935.
RefSeqiNP_001153574.1. NM_001160102.1. [P22079-2]
NP_006142.1. NM_006151.2. [P22079-1]
UniGeneiHs.234742.

3D structure databases

ProteinModelPortaliP22079.
SMRiP22079.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi110207. 1 interactor.
STRINGi9606.ENSP00000262290.

Chemistry databases

BindingDBiP22079.
ChEMBLiCHEMBL5898.

Protein family/group databases

PeroxiBasei3316. HsLPO.

PTM databases

iPTMnetiP22079.
PhosphoSitePlusiP22079.

Polymorphism and mutation databases

BioMutaiLPO.
DMDMi12643419.

Proteomic databases

PaxDbiP22079.
PeptideAtlasiP22079.
PRIDEiP22079.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000262290; ENSP00000262290; ENSG00000167419. [P22079-1]
ENST00000421678; ENSP00000400245; ENSG00000167419. [P22079-2]
ENST00000582328; ENSP00000464636; ENSG00000167419. [P22079-2]
GeneIDi4025.
KEGGihsa:4025.
UCSCiuc002ivt.3. human. [P22079-1]

Organism-specific databases

CTDi4025.
DisGeNETi4025.
GeneCardsiLPO.
HGNCiHGNC:6678. LPO.
HPAiHPA028688.
MIMi150205. gene.
170990. gene.
neXtProtiNX_P22079.
OpenTargetsiENSG00000167419.
PharmGKBiPA30439.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2408. Eukaryota.
ENOG410XPZ3. LUCA.
GeneTreeiENSGT00550000074325.
HOGENOMiHOG000016084.
HOVERGENiHBG000071.
InParanoidiP22079.
KOiK12550.
OMAiSPWASVK.
OrthoDBiEOG091G0236.
PhylomeDBiP22079.
TreeFamiTF314316.

Enzyme and pathway databases

BioCyciZFISH:HS09558-MONOMER.

Miscellaneous databases

ChiTaRSiLPO. human.
GeneWikiiLactoperoxidase.
GenomeRNAii4025.
PROiP22079.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000167419.
CleanExiHS_LPO.
ExpressionAtlasiP22079. baseline and differential.
GenevisibleiP22079. HS.

Family and domain databases

Gene3Di1.10.640.10. 2 hits.
InterProiIPR010255. Haem_peroxidase.
IPR019791. Haem_peroxidase_animal.
IPR029587. LPO.
[Graphical view]
PANTHERiPTHR11475:SF67. PTHR11475:SF67. 1 hit.
PfamiPF03098. An_peroxidase. 1 hit.
[Graphical view]
PRINTSiPR00457. ANPEROXIDASE.
SUPFAMiSSF48113. SSF48113. 1 hit.
PROSITEiPS50292. PEROXIDASE_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPERL_HUMAN
AccessioniPrimary (citable) accession number: P22079
Secondary accession number(s): A5JUY4
, E7EMJ3, Q13408, Q3KNQ2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: January 24, 2001
Last modified: November 2, 2016
This is version 164 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.