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P22072 (3BHS2_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 99. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 2
Alternative name(s):
3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type II
Short name=3-beta-HSD II

Including the following 2 domains:

  1. 3-beta-hydroxy-Delta(5)-steroid dehydrogenase
    EC=1.1.1.145
    Alternative name(s):
    3-beta-hydroxy-5-ene steroid dehydrogenase
    Progesterone reductase
  2. Steroid Delta-isomerase
    EC=5.3.3.1
    Alternative name(s):
    Delta-5-3-ketosteroid isomerase
Gene names
Name:Hsd3b
OrganismRattus norvegicus (Rat)
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length373 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

3-beta-HSD is a bifunctional enzyme, that catalyzes the oxidative conversion of Delta(5)-ene-3-beta-hydroxy steroid, and the oxidative conversion of ketosteroids. The 3-beta-HSD enzymatic system plays a crucial role in the biosynthesis of all classes of hormonal steroids.

Catalytic activity

A 3-beta-hydroxy-Delta(5)-steroid + NAD+ = a 3-oxo-Delta(5)-steroid + NADH.

A 3-oxo-Delta(5)-steroid = a 3-oxo-Delta(4)-steroid.

Pathway

Lipid metabolism; steroid biosynthesis.

Subcellular location

Endoplasmic reticulum membrane; Single-pass membrane protein. Mitochondrion membrane; Single-pass membrane protein.

Tissue specificity

Adrenal glands, testes and ovaries.

Sequence similarities

Belongs to the 3-beta-HSD family.

Caution

Rat 3-beta-HSD type II may possess only one transmembrane domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 3733723 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 2
PRO_0000087788

Regions

Transmembrane288 – 30821Helical; Potential

Sites

Active site1551Proton acceptor By similarity
Binding site1591NAD By similarity

Experimental info

Sequence conflict1151A → T in AAB20228. Ref.2
Sequence conflict1651A → S in AAB20228. Ref.2
Sequence conflict3411E → V in AAB20228. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P22072 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 0F922E9632DE9526

FASTA37342,277
        10         20         30         40         50         60 
MPGWSCLVTG AGGFVGQRII RMLVQEKELQ EVRALDKVFR PETKEEFSKL QTKAKVTMLE 

        70         80         90        100        110        120 
GDILDAQYLR RACQGISVVI HTASVMDFSR VLPRQTILDV NLKGTQNLLE AGIHASVPAF 

       130        140        150        160        170        180 
IYCSTVDVAG PNSYKKTILN GREEEHHEST WSNPYPYSKK MAEKAVLAAN GSILKNGGTL 

       190        200        210        220        230        240 
HTCALRPMYI YGERGQFLSR IIIMALKNKG VLNVTGKFSI VNPVYVGNVA WAHILAARGL 

       250        260        270        280        290        300 
RDPKKSQNIQ GQFYYISDDT PHQSYDDLNC TLSKEWGLRL DSSWSLPLPL LYWLAFLLET 

       310        320        330        340        350        360 
VSFLLRPFYN YRPPFNCHLV TLSNSKFTFS YKKAQRDLGY EPLVSWEEAK QKTSEWIGTL 

       370 
VEQHRETLDT KSQ

« Hide

References

[1]"Characterization of rat 3 beta-hydroxysteroid dehydrogenase/delta 5-delta 4 isomerase cDNAs and differential tissue-specific expression of the corresponding mRNAs in steroidogenic and peripheral tissues."
Zhao H.-F., Labrie C., Simard J., de Launoit Y., Trudel C., Martel C., Rheaume E., Dupont E., Luu-The V., Pelletier G., Labrie F.
J. Biol. Chem. 266:583-593(1991) [PubMed: 1985917] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Regulation of expression of male-specific rat liver microsomal 3 beta-hydroxysteroid dehydrogenase."
Naville D., Keeney D.S., Jenkin G., Murry B.A., Head J.R., Mason J.I.
Mol. Endocrinol. 5:1090-1100(1991) [PubMed: 1944305] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Liver.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		M38179 mRNA. Translation: AAA63475.1.
S63167 mRNA. Translation: AAB20228.1.
IPIIPI00554001.
PIRDERTHM. A40378.
DERTH2. B39051.
RefSeqNP_001036084.1. NM_001042619.1.
UniGeneRn.128814.

3D structure databases

ProteinModelPortalP22072.
ModBaseSearch...

Protein-protein interaction databases

STRINGP22072.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID682974.
KEGGrno:682974.
UCSCM38179. rat.

Organism-specific databases

CTD111785.
RGD1592771. Hsd3b.

Phylogenomic databases

GeneTreeENSGT00550000074557.
HOVERGENHBG000014.
InParanoidP22072.
OrthoDBEOG4K3KWG.
PhylomeDBP22072.

Gene expression databases

ArrayExpressP22072.
GenevestigatorP22072.
GermOnlineENSRNOG00000019454. Rattus norvegicus.

Family and domain databases

InterProIPR002225. 3Beta_OHSteriod_DH/Estase.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
Gene3DG3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PfamPF01073. 3Beta_HSD. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio724140.

Entry information

Entry name3BHS2_RAT
AccessionPrimary (citable) accession number: P22072
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: January 23, 2007
Last modified: December 14, 2011
This is version 99 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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