Reviewed, UniProtKB/Swiss-Prot P22072 (3BHS2_RAT)

Names and origin

Protein names

Recommended name:
    3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 2
Alternative name(s):
    3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type II
      Short name=3-beta-HSD II
Including the following 2 domains:
    1- Recommended name:
            3-beta-hydroxy-Delta(5)-steroid dehydrogenase
              EC=1.1.1.145
        Alternative name(s):
            3-beta-hydroxy-5-ene steroid dehydrogenase
            Progesterone reductase
    2- Recommended name:
            Steroid Delta-isomerase
              EC=5.3.3.1
        Alternative name(s):
            Delta-5-3-ketosteroid isomerase

Gene names

Name:

Hsd3b

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	373 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	3-beta-HSD is a bifunctional enzyme, that catalyzes the oxidative conversion of Delta(5)-ene-3-beta-hydroxy steroid, and the oxidative conversion of ketosteroids. The 3-beta-HSD enzymatic system plays a crucial role in the biosynthesis of all classes of hormonal steroids.
Catalytic activity	A 3-beta-hydroxy-Delta(5)-steroid + NAD+ = a 3-oxo-Delta(5)-steroid + NADH. A 3-oxo-Delta(5)-steroid = a 3-oxo-Delta(4)-steroid.
Pathway	Lipid metabolism; steroid biosynthesis.
Subcellular location	Endoplasmic reticulum membrane; Single-pass membrane protein. Mitochondrion membrane; Single-pass membrane protein.
Tissue specificity	Adrenal glands, testes and ovaries.
Sequence similarities	Belongs to the 3-beta-HSD family.
Caution	Rat 3-beta-HSD type II may possess only one transmembrane domain.

Ontologies

Keywords
Biological process	Steroidogenesis
Cellular component	Endoplasmic reticulum Membrane Mitochondrion
Domain	Transmembrane
Ligand	NAD
Molecular function	Isomerase Oxidoreductase
Technical term	Multifunctional enzyme
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW steroid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell mitochondrion Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	3-beta-hydroxy-delta5-steroid dehydrogenase activity Inferred from electronic annotation. Source: EC binding Inferred from electronic annotation. Source: InterPro steroid delta-isomerase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 373	372	3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 2		PRO_0000087788
Regions
	Transmembrane	288 – 308	21	Potential
Sites
	Active site	155	1	Proton acceptor By similarity
	Binding site	159	1	NAD By similarity
Experimental info
	Sequence conflict	115	1	A → T in AAB20228. Ref.2
	Sequence conflict	165	1	A → S in AAB20228. Ref.2
	Sequence conflict	341	1	E → V in AAB20228. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P22072-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 0F922E9632DE9526	FASTA	373	42,277
	10 20 30 40 50 60 MPGWSCLVTG AGGFVGQRII RMLVQEKELQ EVRALDKVFR PETKEEFSKL QTKAKVTMLE 70 80 90 100 110 120 GDILDAQYLR RACQGISVVI HTASVMDFSR VLPRQTILDV NLKGTQNLLE AGIHASVPAF 130 140 150 160 170 180 IYCSTVDVAG PNSYKKTILN GREEEHHEST WSNPYPYSKK MAEKAVLAAN GSILKNGGTL 190 200 210 220 230 240 HTCALRPMYI YGERGQFLSR IIIMALKNKG VLNVTGKFSI VNPVYVGNVA WAHILAARGL 250 260 270 280 290 300 RDPKKSQNIQ GQFYYISDDT PHQSYDDLNC TLSKEWGLRL DSSWSLPLPL LYWLAFLLET 310 320 330 340 350 360 VSFLLRPFYN YRPPFNCHLV TLSNSKFTFS YKKAQRDLGY EPLVSWEEAK QKTSEWIGTL 370 VEQHRETLDT KSQ

References

[1]	"Characterization of rat 3 beta-hydroxysteroid dehydrogenase/delta 5-delta 4 isomerase cDNAs and differential tissue-specific expression of the corresponding mRNAs in steroidogenic and peripheral tissues." Zhao H.-F., Labrie C., Simard J., de Launoit Y., Trudel C., Martel C., Rheaume E., Dupont E., Luu-The V., Pelletier G., Labrie F. J. Biol. Chem. 266:583-593(1991) [PubMed: 1985917] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Regulation of expression of male-specific rat liver microsomal 3 beta-hydroxysteroid dehydrogenase." Naville D., Keeney D.S., Jenkin G., Murry B.A., Head J.R., Mason J.I. Mol. Endocrinol. 5:1090-1100(1991) [PubMed: 1944305] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M38179 mRNA. Translation: AAA63475.1. S63167 mRNA. Translation: AAB20228.1.
IPI	IPI00554001.
PIR	DERTHM. A40378. DERTH2. B39051.
RefSeq	NP_001036084.1.
UniGene	Rn.128814
3D structure databases
SMR	P22072. Positions 5-364.
ModBase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000056172; ENSRNOP00000053018; ENSRNOG00000019454; Rattus norvegicus. [Genome view]
GeneID	682974.
KEGG	rno:682974.
UCSC	M38179. rat.
Organism-specific databases
CTD	682974.
RGD	1592771. Hsd3b.
Phylogenomic databases
HOVERGEN	P22072.
InParanoid	P22072.
PhylomeDB	P22072.
Enzyme and pathway databases
BRENDA	1.1.1.145. 248. 5.3.3.1. 248.
Gene expression databases
ArrayExpress	P22072.
Genevestigator	P22072.
GermOnline	ENSRNOG00000019454. Rattus norvegicus.
Family and domain databases
InterPro	IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
ProtoNet	Search...
Other Resources
NextBio	724140.

Entry information

Entry name

3BHS2_RAT

Accession

Primary (citable) accession number: P22072

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1991
Last sequence update:	January 23, 2007
Last modified:	February 9, 2010
This is version 84 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)