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Protein

3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 2

Gene

Hsd3b

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

3-beta-HSD is a bifunctional enzyme, that catalyzes the oxidative conversion of Delta(5)-ene-3-beta-hydroxy steroid, and the oxidative conversion of ketosteroids. The 3-beta-HSD enzymatic system plays a crucial role in the biosynthesis of all classes of hormonal steroids.

Catalytic activityi

A 3-beta-hydroxy-Delta(5)-steroid + NAD+ = a 3-oxo-Delta(5)-steroid + NADH.
A 3-oxo-Delta(5)-steroid = a 3-oxo-Delta(4)-steroid.

Pathwayi: steroid biosynthesis

This protein is involved in the pathway steroid biosynthesis, which is part of Lipid metabolism.
View all proteins of this organism that are known to be involved in the pathway steroid biosynthesis and in Lipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei155Proton acceptorBy similarity1
Binding sitei159NADBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Oxidoreductase

Keywords - Biological processi

Steroidogenesis

Keywords - Ligandi

NAD

Enzyme and pathway databases

UniPathwayiUPA00062.

Names & Taxonomyi

Protein namesi
Recommended name:
3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 2
Alternative name(s):
3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type II
Short name:
3-beta-HSD II
Including the following 2 domains:
3-beta-hydroxy-Delta(5)-steroid dehydrogenase (EC:1.1.1.145)
Alternative name(s):
3-beta-hydroxy-5-ene steroid dehydrogenase
Progesterone reductase
Steroid Delta-isomerase (EC:5.3.3.1)
Alternative name(s):
Delta-5-3-ketosteroid isomerase
Gene namesi
Name:Hsd3b
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi1592771. Hsd3b.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei288 – 308HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000877882 – 3733 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 2Add BLAST372

Proteomic databases

PRIDEiP22072.

PTM databases

iPTMnetiP22072.
PhosphoSitePlusiP22072.

Expressioni

Tissue specificityi

Adrenal glands, testes and ovaries.

Structurei

3D structure databases

ProteinModelPortaliP22072.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the 3-beta-HSD family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

HOGENOMiHOG000167989.
HOVERGENiHBG000014.
InParanoidiP22072.
KOiK00070.

Family and domain databases

Gene3Di3.40.50.720. 2 hits.
InterProiIPR002225. 3Beta_OHSteriod_DH/Estase.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF01073. 3Beta_HSD. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 2 hits.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P22072-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPGWSCLVTG AGGFVGQRII RMLVQEKELQ EVRALDKVFR PETKEEFSKL
60 70 80 90 100
QTKAKVTMLE GDILDAQYLR RACQGISVVI HTASVMDFSR VLPRQTILDV
110 120 130 140 150
NLKGTQNLLE AGIHASVPAF IYCSTVDVAG PNSYKKTILN GREEEHHEST
160 170 180 190 200
WSNPYPYSKK MAEKAVLAAN GSILKNGGTL HTCALRPMYI YGERGQFLSR
210 220 230 240 250
IIIMALKNKG VLNVTGKFSI VNPVYVGNVA WAHILAARGL RDPKKSQNIQ
260 270 280 290 300
GQFYYISDDT PHQSYDDLNC TLSKEWGLRL DSSWSLPLPL LYWLAFLLET
310 320 330 340 350
VSFLLRPFYN YRPPFNCHLV TLSNSKFTFS YKKAQRDLGY EPLVSWEEAK
360 370
QKTSEWIGTL VEQHRETLDT KSQ
Length:373
Mass (Da):42,277
Last modified:January 23, 2007 - v3
Checksum:i0F922E9632DE9526
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti115A → T in AAB20228 (PubMed:1944305).Curated1
Sequence conflicti165A → S in AAB20228 (PubMed:1944305).Curated1
Sequence conflicti341E → V in AAB20228 (PubMed:1944305).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M38179 mRNA. Translation: AAA63475.1.
S63167 mRNA. Translation: AAB20228.1.
PIRiA40378. DERTHM.
B39051. DERTH2.
RefSeqiNP_001036084.1. NM_001042619.1.
UniGeneiRn.128814.

Genome annotation databases

GeneIDi682974.
KEGGirno:682974.
UCSCiRGD:1592771. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M38179 mRNA. Translation: AAA63475.1.
S63167 mRNA. Translation: AAB20228.1.
PIRiA40378. DERTHM.
B39051. DERTH2.
RefSeqiNP_001036084.1. NM_001042619.1.
UniGeneiRn.128814.

3D structure databases

ProteinModelPortaliP22072.
ModBaseiSearch...
MobiDBiSearch...

PTM databases

iPTMnetiP22072.
PhosphoSitePlusiP22072.

Proteomic databases

PRIDEiP22072.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi682974.
KEGGirno:682974.
UCSCiRGD:1592771. rat.

Organism-specific databases

CTDi15494.
RGDi1592771. Hsd3b.

Phylogenomic databases

HOGENOMiHOG000167989.
HOVERGENiHBG000014.
InParanoidiP22072.
KOiK00070.

Enzyme and pathway databases

UniPathwayiUPA00062.

Miscellaneous databases

PROiP22072.

Family and domain databases

Gene3Di3.40.50.720. 2 hits.
InterProiIPR002225. 3Beta_OHSteriod_DH/Estase.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF01073. 3Beta_HSD. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 2 hits.
ProtoNetiSearch...

Entry informationi

Entry namei3BHS2_RAT
AccessioniPrimary (citable) accession number: P22072
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 132 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

Rat 3-beta-HSD type II may possess only one transmembrane domain.Curated

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.