P22071 (3BHS1_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1

Alternative name(s):
3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type I
Short name=3-beta-HSD I

Including the following 2 domains:

1. 3-beta-hydroxy-Delta(5)-steroid dehydrogenase
   EC=1.1.1.145
   Alternative name(s):
   3-beta-hydroxy-5-ene steroid dehydrogenase
   Progesterone reductase
2. Steroid Delta-isomerase
   EC=5.3.3.1
   Alternative name(s):
   Delta-5-3-ketosteroid isomerase

Gene names

Name:

Hsd3b1

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	373 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	3-beta-HSD is a bifunctional enzyme, that catalyzes the oxidative conversion of Delta(5)-ene-3-beta-hydroxy steroid, and the oxidative conversion of ketosteroids. The 3-beta-HSD enzymatic system plays a crucial role in the biosynthesis of all classes of hormonal steroids.
Catalytic activity	A 3-beta-hydroxy-Delta(5)-steroid + NAD+ = a 3-oxo-Delta(5)-steroid + NADH. A 3-oxo-Delta(5)-steroid = a 3-oxo-Delta(4)-steroid.
Pathway	Lipid metabolism; steroid biosynthesis.
Subcellular location	Endoplasmic reticulum membrane; Single-pass membrane protein. Mitochondrion membrane; Single-pass membrane protein.
Tissue specificity	Adrenal glands, testes and ovaries.
Sequence similarities	Belongs to the 3-beta-HSD family.

Ontologies

Keywords
Biological process	Steroidogenesis
Cellular component	Endoplasmic reticulum Membrane Mitochondrion
Domain	Transmembrane Transmembrane helix
Ligand	NAD
Molecular function	Isomerase Oxidoreductase
Technical term	Complete proteome Multifunctional enzyme Reference proteome
Gene Ontology (GO)
Biological_process	Leydig cell differentiation Inferred from expression pattern PubMed 18502897. Source: RGD adrenal gland development Inferred from expression pattern PubMed 1935797. Source: RGD biphenyl metabolic process Inferred from expression pattern PubMed 19071209. Source: RGD cellular response to antibiotic Inferred from expression pattern PubMed 18655822. Source: RGD cellular response to cAMP Inferred from expression pattern PubMed 1312436. Source: RGD cellular response to dexamethasone stimulus Inferred from expression pattern PubMed 20075416. Source: RGD cellular response to fibroblast growth factor stimulus Inferred from expression pattern PubMed 17494997. Source: RGD cellular response to follicle-stimulating hormone stimulus Inferred from expression pattern PubMed 20810564. Source: RGD cellular response to glucose stimulus Inferred from expression pattern PubMed 18353182. Source: RGD cellular response to hydrogen peroxide Inferred from expression pattern PubMed 16374549. Source: RGD cellular response to insulin stimulus Inferred from expression pattern PubMed 2177628. Source: RGD cellular response to luteinizing hormone stimulus Inferred from expression pattern PubMed 1312436. Source: RGD cellular response to mercury ion Inferred from expression pattern PubMed 2253967. Source: RGD cellular response to methylmercury Inferred from expression pattern PubMed 2253967. Source: RGD cellular response to transforming growth factor beta stimulus Inferred from expression pattern PubMed 18511507. Source: RGD dibenzo-p-dioxin metabolic process Inferred from expression pattern PubMed 18637154. Source: RGD diterpenoid metabolic process Inferred from expression pattern PubMed 1312436. Source: RGD estrogen biosynthetic process Inferred from expression pattern PubMed 20810564. Source: RGD female gonad development Inferred from expression pattern PubMed 8439611. Source: RGD fractalkine metabolic process Inferred from expression pattern PubMed 18292196. Source: RGD granulocyte differentiation Inferred from expression pattern PubMed 18401763. Source: RGD hippocampus development Inferred from expression pattern PubMed 21047951. Source: RGD insecticide metabolic process Inferred from expression pattern PubMed 18401575. Source: RGD maternal process involved in female pregnancy Inferred from expression pattern PubMed 17257522. Source: RGD organic acid metabolic process Inferred from expression pattern PubMed 17400581. Source: RGD ovulation Inferred from expression pattern PubMed 19775733. Source: RGD phenol-containing compound metabolic process Inferred from expression pattern PubMed 19429456. Source: RGD phosphatidylcholine metabolic process Inferred from expression pattern PubMed 2590715. Source: RGD progesterone biosynthetic process Inferred from direct assay Ref.2. Source: RGD response to L-ascorbic acid Inferred from expression pattern PubMed 17280759. Source: RGD response to activity Inferred from expression pattern PubMed 18923565. Source: RGD response to alkaloid Inferred from expression pattern PubMed 18772241. Source: RGD response to arsenic-containing substance Inferred from expression pattern PubMed 16483355. Source: RGD response to cadmium ion Inferred from expression pattern PubMed 18972398. Source: RGD response to calcium ion Inferred from expression pattern PubMed 16472573. Source: RGD response to corticosterone stimulus Inferred from expression pattern PubMed 18335507. Source: RGD response to drug Inferred from expression pattern PubMed 18772241. Source: RGD response to estradiol stimulus Inferred from expression pattern PubMed 16020475. Source: RGD response to ethanol Inferred from expression pattern PubMed 17193892. Source: RGD response to fatty acid Inferred from expression pattern PubMed 19130109. Source: RGD response to fungicide Inferred from expression pattern PubMed 17485193. Source: RGD response to herbicide Inferred from expression pattern PubMed 20045047. Source: RGD response to lead ion Inferred from expression pattern PubMed 18972398. Source: RGD response to lithium ion Inferred from expression pattern PubMed 14972747. Source: RGD response to vitamin E Inferred from expression pattern PubMed 17280759. Source: RGD testosterone biosynthetic process Inferred from expression pattern PubMed 16467141. Source: RGD toluene metabolic process Inferred from expression pattern PubMed 19110042. Source: RGD
Cellular_component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW mitochondrial crista Inferred from direct assay PubMed 9065457. Source: RGD
Molecular_function	3-beta-hydroxy-delta5-steroid dehydrogenase activity Inferred from direct assay Ref.2. Source: RGD NAD binding Inferred from direct assay Ref.2. Source: RGD dihydrotestosterone 17-beta-dehydrogenase activity Inferred from direct assay PubMed 1309351. Source: RGD steroid binding Inferred from physical interaction Ref.2. Source: RGD steroid delta-isomerase activity Inferred from direct assay Ref.1. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 373	372	3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1		PRO_0000087787
Regions
	Transmembrane	288 – 308	21	Helical; Potential
Sites
	Active site	155	1	Proton acceptor By similarity
	Binding site	159	1	NAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	P22071 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 1BB058B5171C2F24	FASTA	373	42,037
	10 20 30 40 50 60 MPGWSCLVTG AGGFVGQRII RMLVQEKELQ EVRALDKVFR PETKEEFSKL QTKAKVTMLE 70 80 90 100 110 120 GDILDAQYLR RACQGISVVI HTAAVIDVSH VLPRQTILDV NLKGTQNILE ACVEASVPAF 130 140 150 160 170 180 IYCSTVDVAG PNSYKKIILN GHEEEHHEST WSDAYPYSKR MAEKAVLAAN GSILKNGGTL 190 200 210 220 230 240 HTCALRPMYI YGERSPFLSV MILAALKNKG ILNVTGKFSI ANPVYVGNVA WAHILAARGL 250 260 270 280 290 300 RDPKKSQNVQ GQFYYISDDT PHQSYDDLNC TLSKEWGLRL DSSWSLPLPL LYWLAFLLET 310 320 330 340 350 360 VSFLLRPFYN YRPPFNCHLV TLSNSKFTFS YKKAQRDLGY VPLVSWEEAK QKTSEWIGTL 370 VEQHRETLDT KSQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of rat 3 beta-hydroxysteroid dehydrogenase/delta 5-delta 4 isomerase cDNAs and differential tissue-specific expression of the corresponding mRNAs in steroidogenic and peripheral tissues." Zhao H.-F., Labrie C., Simard J., de Launoit Y., Trudel C., Martel C., Rheaume E., Dupont E., Luu-The V., Pelletier G., Labrie F. J. Biol. Chem. 266:583-593(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"3 beta-hydroxysteroid dehydrogenase/delta 5-->4-isomerase expression in rat and characterization of the testis isoform." Lorence M.C., Naville D., Graham-Lorence S.E., Mack S.O., Murry B.A., Trant J.M., Mason J.I. Mol. Cell. Endocrinol. 80:21-31(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Testis.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Ovary.
[4]	Si J.L., Li J., Xu X.Q. Submitted (APR-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 227-358. Strain: Wistar.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M38178 mRNA. Translation: AAA63474.1. BC086578 mRNA. Translation: AAH86578.1. DQ515797 mRNA. Translation: ABF70960.1.
IPI	IPI00362470.
PIR	DERTH1. A39051.
RefSeq	NP_001007720.3. NM_001007719.3.
UniGene	Rn.128814.
3D structure databases
ProteinModelPortal	P22071.
ModBase	Search...
Proteomic databases
PaxDb	P22071.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	360348.
KEGG	rno:360348.
UCSC	RGD:1308676. rat.
Organism-specific databases
CTD	3283.
RGD	1308676. Hsd3b1.
Phylogenomic databases
eggNOG	COG0451.
HOGENOM	HOG000167989.
HOVERGEN	HBG000014.
InParanoid	P22071.
KO	K00070.
OMA	WVFPRIA.
Enzyme and pathway databases
BRENDA	5.3.3.1. 5301.
UniPathway	UPA00062.
Gene expression databases
ArrayExpress	P22071.
Genevestigator	P22071.
GermOnline	ENSRNOG00000019454. Rattus norvegicus.
Family and domain databases
Gene3D	3.40.50.720. 2 hits.
InterPro	IPR002225. 3Beta_OHSteriod_DH/Estase. IPR016040. NAD(P)-bd_dom. [Graphical view]
Pfam	PF01073. 3Beta_HSD. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	672781.

Entry information

Entry name

3BHS1_RAT

Accession

Primary (citable) accession number: P22071
Secondary accession number(s): Q19P43

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1991
Last sequence update:	January 23, 2007
Last modified:	April 3, 2013
This is version 116 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program