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P22071

- 3BHS1_RAT

UniProt

P22071 - 3BHS1_RAT

Protein

3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1

Gene

Hsd3b1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 124 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    3-beta-HSD is a bifunctional enzyme, that catalyzes the oxidative conversion of Delta(5)-ene-3-beta-hydroxy steroid, and the oxidative conversion of ketosteroids. The 3-beta-HSD enzymatic system plays a crucial role in the biosynthesis of all classes of hormonal steroids.

    Catalytic activityi

    A 3-beta-hydroxy-Delta(5)-steroid + NAD+ = a 3-oxo-Delta(5)-steroid + NADH.
    A 3-oxo-Delta(5)-steroid = a 3-oxo-Delta(4)-steroid.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei155 – 1551Proton acceptorBy similarity
    Binding sitei159 – 1591NADBy similarity

    GO - Molecular functioni

    1. 3-beta-hydroxy-delta5-steroid dehydrogenase activity Source: RGD
    2. dihydrotestosterone 17-beta-dehydrogenase activity Source: RGD
    3. NAD binding Source: RGD
    4. steroid binding Source: RGD
    5. steroid delta-isomerase activity Source: RGD

    GO - Biological processi

    1. adrenal gland development Source: RGD
    2. biphenyl metabolic process Source: RGD
    3. C21-steroid hormone metabolic process Source: RGD
    4. cellular response to antibiotic Source: RGD
    5. cellular response to cAMP Source: RGD
    6. cellular response to dexamethasone stimulus Source: RGD
    7. cellular response to fibroblast growth factor stimulus Source: RGD
    8. cellular response to follicle-stimulating hormone stimulus Source: RGD
    9. cellular response to glucose stimulus Source: RGD
    10. cellular response to gonadotropin stimulus Source: RGD
    11. cellular response to hydrogen peroxide Source: RGD
    12. cellular response to insulin stimulus Source: RGD
    13. cellular response to luteinizing hormone stimulus Source: RGD
    14. cellular response to mercury ion Source: RGD
    15. cellular response to methylmercury Source: RGD
    16. cellular response to transforming growth factor beta stimulus Source: RGD
    17. dibenzo-p-dioxin metabolic process Source: RGD
    18. diterpenoid metabolic process Source: RGD
    19. estrogen biosynthetic process Source: RGD
    20. female gonad development Source: RGD
    21. fractalkine metabolic process Source: RGD
    22. granulocyte differentiation Source: RGD
    23. hippocampus development Source: RGD
    24. insecticide metabolic process Source: RGD
    25. Leydig cell differentiation Source: RGD
    26. male gonad development Source: RGD
    27. maternal process involved in female pregnancy Source: RGD
    28. organic acid metabolic process Source: RGD
    29. ovulation Source: RGD
    30. phenol-containing compound metabolic process Source: RGD
    31. phosphatidylcholine metabolic process Source: RGD
    32. progesterone biosynthetic process Source: RGD
    33. response to activity Source: RGD
    34. response to alkaloid Source: RGD
    35. response to arsenic-containing substance Source: RGD
    36. response to cadmium ion Source: RGD
    37. response to calcium ion Source: RGD
    38. response to corticosterone Source: RGD
    39. response to drug Source: RGD
    40. response to estradiol Source: RGD
    41. response to ethanol Source: RGD
    42. response to fatty acid Source: RGD
    43. response to fungicide Source: RGD
    44. response to herbicide Source: RGD
    45. response to L-ascorbic acid Source: RGD
    46. response to lead ion Source: RGD
    47. response to lithium ion Source: RGD
    48. response to metal ion Source: RGD
    49. response to nutrient levels Source: RGD
    50. response to organic cyclic compound Source: RGD
    51. response to organic substance Source: RGD
    52. response to vitamin E Source: RGD
    53. steroid metabolic process Source: RGD
    54. testosterone biosynthetic process Source: RGD
    55. toluene metabolic process Source: RGD

    Keywords - Molecular functioni

    Isomerase, Oxidoreductase

    Keywords - Biological processi

    Steroidogenesis

    Keywords - Ligandi

    NAD

    Enzyme and pathway databases

    BRENDAi5.3.3.1. 5301.
    UniPathwayiUPA00062.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1
    Alternative name(s):
    3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type I
    Short name:
    3-beta-HSD I
    Including the following 2 domains:
    3-beta-hydroxy-Delta(5)-steroid dehydrogenase (EC:1.1.1.145)
    Alternative name(s):
    3-beta-hydroxy-5-ene steroid dehydrogenase
    Progesterone reductase
    Steroid Delta-isomerase (EC:5.3.3.1)
    Alternative name(s):
    Delta-5-3-ketosteroid isomerase
    Gene namesi
    Name:Hsd3b1
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi1308676. Hsd3b1.

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB-KW
    3. intracellular membrane-bounded organelle Source: RGD
    4. mitochondrial crista Source: RGD

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane, Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 3733723 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1PRO_0000087787Add
    BLAST

    Proteomic databases

    PaxDbiP22071.
    PRIDEiP22071.

    Expressioni

    Tissue specificityi

    Adrenal glands, testes and ovaries.

    Gene expression databases

    GenevestigatoriP22071.

    Structurei

    3D structure databases

    ProteinModelPortaliP22071.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei288 – 30821HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the 3-beta-HSD family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0451.
    HOGENOMiHOG000167989.
    HOVERGENiHBG000014.
    InParanoidiP22071.
    KOiK00070.
    PhylomeDBiP22071.

    Family and domain databases

    Gene3Di3.40.50.720. 2 hits.
    InterProiIPR002225. 3Beta_OHSteriod_DH/Estase.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PfamiPF01073. 3Beta_HSD. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P22071-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPGWSCLVTG AGGFVGQRII RMLVQEKELQ EVRALDKVFR PETKEEFSKL    50
    QTKAKVTMLE GDILDAQYLR RACQGISVVI HTAAVIDVSH VLPRQTILDV 100
    NLKGTQNILE ACVEASVPAF IYCSTVDVAG PNSYKKIILN GHEEEHHEST 150
    WSDAYPYSKR MAEKAVLAAN GSILKNGGTL HTCALRPMYI YGERSPFLSV 200
    MILAALKNKG ILNVTGKFSI ANPVYVGNVA WAHILAARGL RDPKKSQNVQ 250
    GQFYYISDDT PHQSYDDLNC TLSKEWGLRL DSSWSLPLPL LYWLAFLLET 300
    VSFLLRPFYN YRPPFNCHLV TLSNSKFTFS YKKAQRDLGY VPLVSWEEAK 350
    QKTSEWIGTL VEQHRETLDT KSQ 373
    Length:373
    Mass (Da):42,037
    Last modified:January 23, 2007 - v3
    Checksum:i1BB058B5171C2F24
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M38178 mRNA. Translation: AAA63474.1.
    BC086578 mRNA. Translation: AAH86578.1.
    DQ515797 mRNA. Translation: ABF70960.1.
    PIRiA39051. DERTH1.
    RefSeqiNP_001007720.3. NM_001007719.3.
    UniGeneiRn.128814.

    Genome annotation databases

    GeneIDi360348.
    KEGGirno:360348.
    UCSCiRGD:1308676. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M38178 mRNA. Translation: AAA63474.1 .
    BC086578 mRNA. Translation: AAH86578.1 .
    DQ515797 mRNA. Translation: ABF70960.1 .
    PIRi A39051. DERTH1.
    RefSeqi NP_001007720.3. NM_001007719.3.
    UniGenei Rn.128814.

    3D structure databases

    ProteinModelPortali P22071.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PaxDbi P22071.
    PRIDEi P22071.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 360348.
    KEGGi rno:360348.
    UCSCi RGD:1308676. rat.

    Organism-specific databases

    CTDi 3283.
    RGDi 1308676. Hsd3b1.

    Phylogenomic databases

    eggNOGi COG0451.
    HOGENOMi HOG000167989.
    HOVERGENi HBG000014.
    InParanoidi P22071.
    KOi K00070.
    PhylomeDBi P22071.

    Enzyme and pathway databases

    UniPathwayi UPA00062 .
    BRENDAi 5.3.3.1. 5301.

    Miscellaneous databases

    NextBioi 672781.

    Gene expression databases

    Genevestigatori P22071.

    Family and domain databases

    Gene3Di 3.40.50.720. 2 hits.
    InterProi IPR002225. 3Beta_OHSteriod_DH/Estase.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view ]
    Pfami PF01073. 3Beta_HSD. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Characterization of rat 3 beta-hydroxysteroid dehydrogenase/delta 5-delta 4 isomerase cDNAs and differential tissue-specific expression of the corresponding mRNAs in steroidogenic and peripheral tissues."
      Zhao H.-F., Labrie C., Simard J., de Launoit Y., Trudel C., Martel C., Rheaume E., Dupont E., Luu-The V., Pelletier G., Labrie F.
      J. Biol. Chem. 266:583-593(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "3 beta-hydroxysteroid dehydrogenase/delta 5-->4-isomerase expression in rat and characterization of the testis isoform."
      Lorence M.C., Naville D., Graham-Lorence S.E., Mack S.O., Murry B.A., Trant J.M., Mason J.I.
      Mol. Cell. Endocrinol. 80:21-31(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Testis.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Ovary.
    4. Si J.L., Li J., Xu X.Q.
      Submitted (APR-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 227-358.
      Strain: Wistar.

    Entry informationi

    Entry namei3BHS1_RAT
    AccessioniPrimary (citable) accession number: P22071
    Secondary accession number(s): Q19P43
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1991
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 124 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Multifunctional enzyme, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3