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P22071

- 3BHS1_RAT

UniProt

P22071 - 3BHS1_RAT

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Protein

3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1

Gene

Hsd3b1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

3-beta-HSD is a bifunctional enzyme, that catalyzes the oxidative conversion of Delta(5)-ene-3-beta-hydroxy steroid, and the oxidative conversion of ketosteroids. The 3-beta-HSD enzymatic system plays a crucial role in the biosynthesis of all classes of hormonal steroids.

Catalytic activityi

A 3-beta-hydroxy-Delta(5)-steroid + NAD+ = a 3-oxo-Delta(5)-steroid + NADH.
A 3-oxo-Delta(5)-steroid = a 3-oxo-Delta(4)-steroid.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei155 – 1551Proton acceptorBy similarity
Binding sitei159 – 1591NADBy similarity

GO - Molecular functioni

  1. 3-beta-hydroxy-delta5-steroid dehydrogenase activity Source: RGD
  2. dihydrotestosterone 17-beta-dehydrogenase activity Source: RGD
  3. NAD binding Source: RGD
  4. steroid binding Source: RGD
  5. steroid delta-isomerase activity Source: RGD

GO - Biological processi

  1. adrenal gland development Source: RGD
  2. biphenyl metabolic process Source: RGD
  3. C21-steroid hormone metabolic process Source: RGD
  4. cellular response to antibiotic Source: RGD
  5. cellular response to cAMP Source: RGD
  6. cellular response to dexamethasone stimulus Source: RGD
  7. cellular response to fibroblast growth factor stimulus Source: RGD
  8. cellular response to follicle-stimulating hormone stimulus Source: RGD
  9. cellular response to glucose stimulus Source: RGD
  10. cellular response to gonadotropin stimulus Source: RGD
  11. cellular response to hydrogen peroxide Source: RGD
  12. cellular response to insulin stimulus Source: RGD
  13. cellular response to luteinizing hormone stimulus Source: RGD
  14. cellular response to mercury ion Source: RGD
  15. cellular response to methylmercury Source: RGD
  16. cellular response to transforming growth factor beta stimulus Source: RGD
  17. dibenzo-p-dioxin metabolic process Source: RGD
  18. diterpenoid metabolic process Source: RGD
  19. estrogen biosynthetic process Source: RGD
  20. female gonad development Source: RGD
  21. fractalkine metabolic process Source: RGD
  22. granulocyte differentiation Source: RGD
  23. hippocampus development Source: RGD
  24. insecticide metabolic process Source: RGD
  25. Leydig cell differentiation Source: RGD
  26. male gonad development Source: RGD
  27. maternal process involved in female pregnancy Source: RGD
  28. organic acid metabolic process Source: RGD
  29. ovulation Source: RGD
  30. phenol-containing compound metabolic process Source: RGD
  31. phosphatidylcholine metabolic process Source: RGD
  32. progesterone biosynthetic process Source: RGD
  33. response to activity Source: RGD
  34. response to alkaloid Source: RGD
  35. response to arsenic-containing substance Source: RGD
  36. response to cadmium ion Source: RGD
  37. response to calcium ion Source: RGD
  38. response to corticosterone Source: RGD
  39. response to drug Source: RGD
  40. response to estradiol Source: RGD
  41. response to ethanol Source: RGD
  42. response to fatty acid Source: RGD
  43. response to fungicide Source: RGD
  44. response to herbicide Source: RGD
  45. response to L-ascorbic acid Source: RGD
  46. response to lead ion Source: RGD
  47. response to lithium ion Source: RGD
  48. response to metal ion Source: RGD
  49. response to nutrient levels Source: RGD
  50. response to organic cyclic compound Source: RGD
  51. response to organic substance Source: RGD
  52. response to vitamin E Source: RGD
  53. steroid metabolic process Source: RGD
  54. testosterone biosynthetic process Source: RGD
  55. toluene metabolic process Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Oxidoreductase

Keywords - Biological processi

Steroidogenesis

Keywords - Ligandi

NAD

Enzyme and pathway databases

BRENDAi5.3.3.1. 5301.
UniPathwayiUPA00062.

Names & Taxonomyi

Protein namesi
Recommended name:
3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1
Alternative name(s):
3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type I
Short name:
3-beta-HSD I
Including the following 2 domains:
3-beta-hydroxy-Delta(5)-steroid dehydrogenase (EC:1.1.1.145)
Alternative name(s):
3-beta-hydroxy-5-ene steroid dehydrogenase
Progesterone reductase
Steroid Delta-isomerase (EC:5.3.3.1)
Alternative name(s):
Delta-5-3-ketosteroid isomerase
Gene namesi
Name:Hsd3b1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi1308676. Hsd3b1.

Subcellular locationi

GO - Cellular componenti

  1. endoplasmic reticulum Source: UniProtKB-KW
  2. integral component of membrane Source: UniProtKB-KW
  3. intracellular membrane-bounded organelle Source: RGD
  4. mitochondrial crista Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 3733723 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1PRO_0000087787Add
BLAST

Proteomic databases

PaxDbiP22071.
PRIDEiP22071.

Expressioni

Tissue specificityi

Adrenal glands, testes and ovaries.

Gene expression databases

GenevestigatoriP22071.

Structurei

3D structure databases

ProteinModelPortaliP22071.
ModBaseiSearch...
MobiDBiSearch...

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei288 – 30821HelicalSequence AnalysisAdd
BLAST

Family & Domainsi

Sequence similaritiesi

Belongs to the 3-beta-HSD family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0451.
HOGENOMiHOG000167989.
HOVERGENiHBG000014.
InParanoidiP22071.
KOiK00070.
PhylomeDBiP22071.

Family and domain databases

Gene3Di3.40.50.720. 2 hits.
InterProiIPR002225. 3Beta_OHSteriod_DH/Estase.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF01073. 3Beta_HSD. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P22071-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPGWSCLVTG AGGFVGQRII RMLVQEKELQ EVRALDKVFR PETKEEFSKL
60 70 80 90 100
QTKAKVTMLE GDILDAQYLR RACQGISVVI HTAAVIDVSH VLPRQTILDV
110 120 130 140 150
NLKGTQNILE ACVEASVPAF IYCSTVDVAG PNSYKKIILN GHEEEHHEST
160 170 180 190 200
WSDAYPYSKR MAEKAVLAAN GSILKNGGTL HTCALRPMYI YGERSPFLSV
210 220 230 240 250
MILAALKNKG ILNVTGKFSI ANPVYVGNVA WAHILAARGL RDPKKSQNVQ
260 270 280 290 300
GQFYYISDDT PHQSYDDLNC TLSKEWGLRL DSSWSLPLPL LYWLAFLLET
310 320 330 340 350
VSFLLRPFYN YRPPFNCHLV TLSNSKFTFS YKKAQRDLGY VPLVSWEEAK
360 370
QKTSEWIGTL VEQHRETLDT KSQ
Length:373
Mass (Da):42,037
Last modified:January 23, 2007 - v3
Checksum:i1BB058B5171C2F24
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M38178 mRNA. Translation: AAA63474.1.
BC086578 mRNA. Translation: AAH86578.1.
DQ515797 mRNA. Translation: ABF70960.1.
PIRiA39051. DERTH1.
RefSeqiNP_001007720.3. NM_001007719.3.
UniGeneiRn.128814.

Genome annotation databases

GeneIDi360348.
KEGGirno:360348.
UCSCiRGD:1308676. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M38178 mRNA. Translation: AAA63474.1 .
BC086578 mRNA. Translation: AAH86578.1 .
DQ515797 mRNA. Translation: ABF70960.1 .
PIRi A39051. DERTH1.
RefSeqi NP_001007720.3. NM_001007719.3.
UniGenei Rn.128814.

3D structure databases

ProteinModelPortali P22071.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PaxDbi P22071.
PRIDEi P22071.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 360348.
KEGGi rno:360348.
UCSCi RGD:1308676. rat.

Organism-specific databases

CTDi 3283.
RGDi 1308676. Hsd3b1.

Phylogenomic databases

eggNOGi COG0451.
HOGENOMi HOG000167989.
HOVERGENi HBG000014.
InParanoidi P22071.
KOi K00070.
PhylomeDBi P22071.

Enzyme and pathway databases

UniPathwayi UPA00062 .
BRENDAi 5.3.3.1. 5301.

Miscellaneous databases

NextBioi 672781.

Gene expression databases

Genevestigatori P22071.

Family and domain databases

Gene3Di 3.40.50.720. 2 hits.
InterProi IPR002225. 3Beta_OHSteriod_DH/Estase.
IPR016040. NAD(P)-bd_dom.
[Graphical view ]
Pfami PF01073. 3Beta_HSD. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of rat 3 beta-hydroxysteroid dehydrogenase/delta 5-delta 4 isomerase cDNAs and differential tissue-specific expression of the corresponding mRNAs in steroidogenic and peripheral tissues."
    Zhao H.-F., Labrie C., Simard J., de Launoit Y., Trudel C., Martel C., Rheaume E., Dupont E., Luu-The V., Pelletier G., Labrie F.
    J. Biol. Chem. 266:583-593(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "3 beta-hydroxysteroid dehydrogenase/delta 5-->4-isomerase expression in rat and characterization of the testis isoform."
    Lorence M.C., Naville D., Graham-Lorence S.E., Mack S.O., Murry B.A., Trant J.M., Mason J.I.
    Mol. Cell. Endocrinol. 80:21-31(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Testis.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Ovary.
  4. Si J.L., Li J., Xu X.Q.
    Submitted (APR-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 227-358.
    Strain: Wistar.

Entry informationi

Entry namei3BHS1_RAT
AccessioniPrimary (citable) accession number: P22071
Secondary accession number(s): Q19P43
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: January 23, 2007
Last modified: October 29, 2014
This is version 125 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3