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Protein

3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1

Gene

Hsd3b1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

3-beta-HSD is a bifunctional enzyme, that catalyzes the oxidative conversion of Delta5-ene-3-beta-hydroxy steroid, and the oxidative conversion of ketosteroids. The 3-beta-HSD enzymatic system plays a crucial role in the biosynthesis of all classes of hormonal steroids.

Catalytic activityi

A 3-beta-hydroxy-Delta5-steroid + NAD+ = a 3-oxo-Delta5-steroid + NADH.
A 3-oxo-Delta5-steroid = a 3-oxo-Delta4-steroid.

Pathwayi: steroid biosynthesis

This protein is involved in the pathway steroid biosynthesis, which is part of Lipid metabolism.
View all proteins of this organism that are known to be involved in the pathway steroid biosynthesis and in Lipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei155Proton acceptorBy similarity1
Binding sitei159NADBy similarity1

GO - Molecular functioni

  • 3-beta-hydroxy-delta5-steroid dehydrogenase activity Source: RGD
  • dihydrotestosterone 17-beta-dehydrogenase activity Source: RGD
  • NAD binding Source: RGD
  • steroid binding Source: RGD
  • steroid delta-isomerase activity Source: RGD

GO - Biological processi

  • adrenal gland development Source: RGD
  • biphenyl metabolic process Source: RGD
  • C21-steroid hormone metabolic process Source: RGD
  • cellular response to antibiotic Source: RGD
  • cellular response to cAMP Source: RGD
  • cellular response to dexamethasone stimulus Source: RGD
  • cellular response to fibroblast growth factor stimulus Source: RGD
  • cellular response to follicle-stimulating hormone stimulus Source: RGD
  • cellular response to glucose stimulus Source: RGD
  • cellular response to gonadotropin stimulus Source: RGD
  • cellular response to hydrogen peroxide Source: RGD
  • cellular response to insulin stimulus Source: RGD
  • cellular response to luteinizing hormone stimulus Source: RGD
  • cellular response to mercury ion Source: RGD
  • cellular response to methylmercury Source: RGD
  • cellular response to transforming growth factor beta stimulus Source: RGD
  • dibenzo-p-dioxin metabolic process Source: RGD
  • diterpenoid metabolic process Source: RGD
  • female gonad development Source: RGD
  • granulocyte differentiation Source: RGD
  • hippocampus development Source: RGD
  • insecticide metabolic process Source: RGD
  • Leydig cell differentiation Source: RGD
  • male gonad development Source: RGD
  • maternal process involved in female pregnancy Source: RGD
  • organic acid metabolic process Source: RGD
  • ovulation Source: RGD
  • phenol-containing compound metabolic process Source: RGD
  • phosphatidylcholine metabolic process Source: RGD
  • progesterone biosynthetic process Source: RGD
  • response to activity Source: RGD
  • response to alkaloid Source: RGD
  • response to arsenic-containing substance Source: RGD
  • response to cadmium ion Source: RGD
  • response to calcium ion Source: RGD
  • response to corticosterone Source: RGD
  • response to drug Source: RGD
  • response to estradiol Source: RGD
  • response to ethanol Source: RGD
  • response to fatty acid Source: RGD
  • response to fungicide Source: RGD
  • response to herbicide Source: RGD
  • response to L-ascorbic acid Source: RGD
  • response to lead ion Source: RGD
  • response to lithium ion Source: RGD
  • response to metal ion Source: RGD
  • response to nutrient levels Source: RGD
  • response to organic cyclic compound Source: RGD
  • response to organic substance Source: RGD
  • response to vitamin E Source: RGD
  • steroid metabolic process Source: RGD
  • testosterone biosynthetic process Source: RGD
  • toluene metabolic process Source: RGD

Keywordsi

Molecular functionIsomerase, Oxidoreductase
Biological processSteroidogenesis
LigandNAD

Enzyme and pathway databases

BRENDAi1.1.1.145. 5301.
5.3.3.1. 5301.
UniPathwayiUPA00062.

Names & Taxonomyi

Protein namesi
Recommended name:
3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1
Alternative name(s):
3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type I
Short name:
3-beta-HSD I
Including the following 2 domains:
3-beta-hydroxy-Delta(5)-steroid dehydrogenase (EC:1.1.1.145)
Alternative name(s):
3-beta-hydroxy-5-ene steroid dehydrogenase
Progesterone reductase
Steroid Delta-isomerase (EC:5.3.3.1)
Alternative name(s):
Delta-5-3-ketosteroid isomerase
Gene namesi
Name:Hsd3b1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi1308676. Hsd3b1.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei288 – 308HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

  • endoplasmic reticulum membrane Source: UniProtKB-SubCell
  • integral component of membrane Source: UniProtKB-KW
  • intracellular membrane-bounded organelle Source: RGD
  • mitochondrial crista Source: RGD

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000877871 – 3733 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1Add BLAST373

Proteomic databases

PRIDEiP22071.

Expressioni

Tissue specificityi

Adrenal glands, testes and ovaries.

Structurei

3D structure databases

ProteinModelPortaliP22071.
SMRiP22071.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the 3-beta-HSD family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

HOGENOMiHOG000167989.
HOVERGENiHBG000014.
InParanoidiP22071.
KOiK00070.
PhylomeDBiP22071.

Family and domain databases

InterProiView protein in InterPro
IPR002225. 3Beta_OHSteriod_DH/Estase.
IPR016040. NAD(P)-bd_dom.
PfamiView protein in Pfam
PF01073. 3Beta_HSD. 1 hit.
SUPFAMiSSF51735. SSF51735. 2 hits.

Sequencei

Sequence statusi: Complete.

P22071-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPGWSCLVTG AGGFVGQRII RMLVQEKELQ EVRALDKVFR PETKEEFSKL
60 70 80 90 100
QTKAKVTMLE GDILDAQYLR RACQGISVVI HTAAVIDVSH VLPRQTILDV
110 120 130 140 150
NLKGTQNILE ACVEASVPAF IYCSTVDVAG PNSYKKIILN GHEEEHHEST
160 170 180 190 200
WSDAYPYSKR MAEKAVLAAN GSILKNGGTL HTCALRPMYI YGERSPFLSV
210 220 230 240 250
MILAALKNKG ILNVTGKFSI ANPVYVGNVA WAHILAARGL RDPKKSQNVQ
260 270 280 290 300
GQFYYISDDT PHQSYDDLNC TLSKEWGLRL DSSWSLPLPL LYWLAFLLET
310 320 330 340 350
VSFLLRPFYN YRPPFNCHLV TLSNSKFTFS YKKAQRDLGY VPLVSWEEAK
360 370
QKTSEWIGTL VEQHRETLDT KSQ
Length:373
Mass (Da):42,037
Last modified:January 23, 2007 - v3
Checksum:i1BB058B5171C2F24
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M38178 mRNA. Translation: AAA63474.1.
BC086578 mRNA. Translation: AAH86578.1.
DQ515797 mRNA. Translation: ABF70960.1.
PIRiA39051. DERTH1.
RefSeqiNP_001007720.3. NM_001007719.3.
UniGeneiRn.128814.

Genome annotation databases

GeneIDi360348.
KEGGirno:360348.
UCSCiRGD:1308676. rat.

Similar proteinsi

Entry informationi

Entry namei3BHS1_RAT
AccessioniPrimary (citable) accession number: P22071
Secondary accession number(s): Q19P43
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: January 23, 2007
Last modified: August 30, 2017
This is version 138 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families