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P22071 (3BHS1_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 123. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1
Alternative name(s):
3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type I
Short name=3-beta-HSD I

Including the following 2 domains:

  1. 3-beta-hydroxy-Delta(5)-steroid dehydrogenase
    EC=1.1.1.145
    Alternative name(s):
    3-beta-hydroxy-5-ene steroid dehydrogenase
    Progesterone reductase
  2. Steroid Delta-isomerase
    EC=5.3.3.1
    Alternative name(s):
    Delta-5-3-ketosteroid isomerase
Gene names
Name:Hsd3b1
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length373 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

3-beta-HSD is a bifunctional enzyme, that catalyzes the oxidative conversion of Delta(5)-ene-3-beta-hydroxy steroid, and the oxidative conversion of ketosteroids. The 3-beta-HSD enzymatic system plays a crucial role in the biosynthesis of all classes of hormonal steroids.

Catalytic activity

A 3-beta-hydroxy-Delta(5)-steroid + NAD+ = a 3-oxo-Delta(5)-steroid + NADH.

A 3-oxo-Delta(5)-steroid = a 3-oxo-Delta(4)-steroid.

Pathway

Lipid metabolism; steroid biosynthesis.

Subcellular location

Endoplasmic reticulum membrane; Single-pass membrane protein. Mitochondrion membrane; Single-pass membrane protein.

Tissue specificity

Adrenal glands, testes and ovaries.

Sequence similarities

Belongs to the 3-beta-HSD family.

Ontologies

Keywords
   Biological processSteroidogenesis
   Cellular componentEndoplasmic reticulum
Membrane
Mitochondrion
   DomainTransmembrane
Transmembrane helix
   LigandNAD
   Molecular functionIsomerase
Oxidoreductase
   Technical termComplete proteome
Multifunctional enzyme
Reference proteome
Gene Ontology (GO)
   Biological_processC21-steroid hormone metabolic process

Inferred from direct assay PubMed 19190754. Source: RGD

Leydig cell differentiation

Inferred from expression pattern PubMed 18502897. Source: RGD

adrenal gland development

Inferred from expression pattern PubMed 1935797. Source: RGD

biphenyl metabolic process

Inferred from expression pattern PubMed 19071209. Source: RGD

cellular response to antibiotic

Inferred from expression pattern PubMed 18655822. Source: RGD

cellular response to cAMP

Inferred from expression pattern PubMed 1312436. Source: RGD

cellular response to dexamethasone stimulus

Inferred from expression pattern PubMed 20075416. Source: RGD

cellular response to fibroblast growth factor stimulus

Inferred from expression pattern PubMed 17494997. Source: RGD

cellular response to follicle-stimulating hormone stimulus

Inferred from expression pattern PubMed 20810564. Source: RGD

cellular response to glucose stimulus

Inferred from expression pattern PubMed 18353182. Source: RGD

cellular response to gonadotropin stimulus

Inferred from expression pattern PubMed 8142315. Source: RGD

cellular response to hydrogen peroxide

Inferred from expression pattern PubMed 16374549. Source: RGD

cellular response to insulin stimulus

Inferred from expression pattern PubMed 2177628. Source: RGD

cellular response to luteinizing hormone stimulus

Inferred from expression pattern PubMed 1312436. Source: RGD

cellular response to mercury ion

Inferred from expression pattern PubMed 2253967. Source: RGD

cellular response to methylmercury

Inferred from expression pattern PubMed 2253967. Source: RGD

cellular response to transforming growth factor beta stimulus

Inferred from expression pattern PubMed 18511507. Source: RGD

dibenzo-p-dioxin metabolic process

Inferred from expression pattern PubMed 18637154. Source: RGD

diterpenoid metabolic process

Inferred from expression pattern PubMed 1312436. Source: RGD

estrogen biosynthetic process

Inferred from expression pattern PubMed 20810564. Source: RGD

female gonad development

Inferred from expression pattern PubMed 8439611. Source: RGD

fractalkine metabolic process

Inferred from expression pattern PubMed 18292196. Source: RGD

granulocyte differentiation

Inferred from expression pattern PubMed 18401763. Source: RGD

hippocampus development

Inferred from expression pattern PubMed 21047951. Source: RGD

insecticide metabolic process

Inferred from expression pattern PubMed 18401575. Source: RGD

male gonad development

Inferred from expression pattern PubMed 18772241. Source: RGD

maternal process involved in female pregnancy

Inferred from expression pattern PubMed 17257522. Source: RGD

organic acid metabolic process

Inferred from expression pattern PubMed 17400581. Source: RGD

ovulation

Inferred from expression pattern PubMed 19775733. Source: RGD

phenol-containing compound metabolic process

Inferred from expression pattern PubMed 19429456. Source: RGD

phosphatidylcholine metabolic process

Inferred from expression pattern PubMed 2590715. Source: RGD

progesterone biosynthetic process

Inferred from direct assay Ref.2. Source: RGD

response to L-ascorbic acid

Inferred from expression pattern PubMed 17280759. Source: RGD

response to activity

Inferred from expression pattern PubMed 18923565. Source: RGD

response to alkaloid

Inferred from expression pattern PubMed 18772241. Source: RGD

response to arsenic-containing substance

Inferred from expression pattern PubMed 16483355. Source: RGD

response to cadmium ion

Inferred from expression pattern PubMed 18972398. Source: RGD

response to calcium ion

Inferred from expression pattern PubMed 16472573. Source: RGD

response to corticosterone

Inferred from expression pattern PubMed 18335507. Source: RGD

response to drug

Inferred from expression pattern PubMed 18772241. Source: RGD

response to estradiol

Inferred from expression pattern PubMed 16020475. Source: RGD

response to ethanol

Inferred from expression pattern PubMed 17193892. Source: RGD

response to fatty acid

Inferred from expression pattern PubMed 19130109. Source: RGD

response to fungicide

Inferred from expression pattern PubMed 17485193. Source: RGD

response to herbicide

Inferred from expression pattern PubMed 20045047. Source: RGD

response to lead ion

Inferred from expression pattern PubMed 18972398. Source: RGD

response to lithium ion

Inferred from expression pattern PubMed 14972747. Source: RGD

response to metal ion

Inferred from expression pattern PubMed 17822870. Source: RGD

response to nutrient levels

Inferred from expression pattern PubMed 19130109. Source: RGD

response to organic cyclic compound

Inferred from expression pattern PubMed 18606229. Source: RGD

response to organic substance

Inferred from expression pattern PubMed 18804513. Source: RGD

response to vitamin E

Inferred from expression pattern PubMed 17280759. Source: RGD

steroid metabolic process

Inferred from direct assay Ref.2. Source: RGD

testosterone biosynthetic process

Inferred from expression pattern PubMed 16467141. Source: RGD

toluene metabolic process

Inferred from expression pattern PubMed 19110042. Source: RGD

   Cellular_componentendoplasmic reticulum membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

integral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

intracellular membrane-bounded organelle

Inferred from direct assay PubMed 17936465PubMed 1944305. Source: RGD

mitochondrial crista

Inferred from direct assay PubMed 9065457. Source: RGD

   Molecular_function3-beta-hydroxy-delta5-steroid dehydrogenase activity

Inferred from direct assay Ref.2. Source: RGD

NAD binding

Inferred from direct assay Ref.2. Source: RGD

dihydrotestosterone 17-beta-dehydrogenase activity

Inferred from direct assay PubMed 1309351. Source: RGD

steroid binding

Inferred from physical interaction Ref.2. Source: RGD

steroid delta-isomerase activity

Inferred from direct assay Ref.1. Source: RGD

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 3733723 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1
PRO_0000087787

Regions

Transmembrane288 – 30821Helical; Potential

Sites

Active site1551Proton acceptor By similarity
Binding site1591NAD By similarity

Sequences

Sequence LengthMass (Da)Tools
P22071 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 1BB058B5171C2F24

FASTA37342,037
        10         20         30         40         50         60 
MPGWSCLVTG AGGFVGQRII RMLVQEKELQ EVRALDKVFR PETKEEFSKL QTKAKVTMLE 

        70         80         90        100        110        120 
GDILDAQYLR RACQGISVVI HTAAVIDVSH VLPRQTILDV NLKGTQNILE ACVEASVPAF 

       130        140        150        160        170        180 
IYCSTVDVAG PNSYKKIILN GHEEEHHEST WSDAYPYSKR MAEKAVLAAN GSILKNGGTL 

       190        200        210        220        230        240 
HTCALRPMYI YGERSPFLSV MILAALKNKG ILNVTGKFSI ANPVYVGNVA WAHILAARGL 

       250        260        270        280        290        300 
RDPKKSQNVQ GQFYYISDDT PHQSYDDLNC TLSKEWGLRL DSSWSLPLPL LYWLAFLLET 

       310        320        330        340        350        360 
VSFLLRPFYN YRPPFNCHLV TLSNSKFTFS YKKAQRDLGY VPLVSWEEAK QKTSEWIGTL 

       370 
VEQHRETLDT KSQ 

« Hide

References

« Hide 'large scale' references
[1]"Characterization of rat 3 beta-hydroxysteroid dehydrogenase/delta 5-delta 4 isomerase cDNAs and differential tissue-specific expression of the corresponding mRNAs in steroidogenic and peripheral tissues."
Zhao H.-F., Labrie C., Simard J., de Launoit Y., Trudel C., Martel C., Rheaume E., Dupont E., Luu-The V., Pelletier G., Labrie F.
J. Biol. Chem. 266:583-593(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"3 beta-hydroxysteroid dehydrogenase/delta 5-->4-isomerase expression in rat and characterization of the testis isoform."
Lorence M.C., Naville D., Graham-Lorence S.E., Mack S.O., Murry B.A., Trant J.M., Mason J.I.
Mol. Cell. Endocrinol. 80:21-31(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Testis.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Ovary.
[4]Si J.L., Li J., Xu X.Q.
Submitted (APR-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 227-358.
Strain: Wistar.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M38178 mRNA. Translation: AAA63474.1.
BC086578 mRNA. Translation: AAH86578.1.
DQ515797 mRNA. Translation: ABF70960.1.
PIRDERTH1. A39051.
RefSeqNP_001007720.3. NM_001007719.3.
UniGeneRn.128814.

3D structure databases

ProteinModelPortalP22071.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PaxDbP22071.
PRIDEP22071.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID360348.
KEGGrno:360348.
UCSCRGD:1308676. rat.

Organism-specific databases

CTD3283.
RGD1308676. Hsd3b1.

Phylogenomic databases

eggNOGCOG0451.
HOGENOMHOG000167989.
HOVERGENHBG000014.
InParanoidP22071.
KOK00070.
PhylomeDBP22071.

Enzyme and pathway databases

BRENDA5.3.3.1. 5301.
UniPathwayUPA00062.

Gene expression databases

GenevestigatorP22071.

Family and domain databases

Gene3D3.40.50.720. 2 hits.
InterProIPR002225. 3Beta_OHSteriod_DH/Estase.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamPF01073. 3Beta_HSD. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio672781.

Entry information

Entry name3BHS1_RAT
AccessionPrimary (citable) accession number: P22071
Secondary accession number(s): Q19P43
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: January 23, 2007
Last modified: April 16, 2014
This is version 123 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways