Reviewed, UniProtKB/Swiss-Prot P22071 (3BHS1_RAT)

Names and origin

Protein names

Recommended name:
    3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1
      Short name=3-beta-HSD I
Including the following 2 domains:
    1- Recommended name:
            3-beta-hydroxy-Delta(5)-steroid dehydrogenase
              EC=1.1.1.145
        Alternative name(s):
            3-beta-hydroxy-5-ene steroid dehydrogenase
            Progesterone reductase
    2- Recommended name:
            Steroid Delta-isomerase
              EC=5.3.3.1
        Alternative name(s):
            Delta-5-3-ketosteroid isomerase

Gene names

Name:

Hsd3b1

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	373 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	3-beta-HSD is a bifunctional enzyme, that catalyzes the oxidative conversion of Delta(5)-ene-3-beta-hydroxy steroid, and the oxidative conversion of ketosteroids. The 3-beta-HSD enzymatic system plays a crucial role in the biosynthesis of all classes of hormonal steroids.
Catalytic activity	A 3-beta-hydroxy-Delta(5)-steroid + NAD+ = a 3-oxo-Delta(5)-steroid + NADH. A 3-oxo-Delta(5)-steroid = a 3-oxo-Delta(4)-steroid.
Pathway	Lipid metabolism; steroid biosynthesis.
Subcellular location	Endoplasmic reticulum membrane; Single-pass membrane protein. Mitochondrion membrane; Single-pass membrane protein.
Tissue specificity	Adrenal glands, testes and ovaries.
Sequence similarities	Belongs to the 3-beta-HSD family.

Ontologies

Keywords
Biological process	Steroidogenesis
Cellular component	Endoplasmic reticulum Membrane Mitochondrion
Domain	Transmembrane
Ligand	NAD
Molecular function	Isomerase Oxidoreductase
Technical term	Multifunctional enzyme
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW progesterone biosynthetic process Ref.2 Inferred from direct assay. Source: RGD
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW microsome Inferred from direct assay. Source: RGD mitochondrial membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-beta-hydroxy-delta5-steroid dehydrogenase activity Ref.2 Inferred from direct assay. Source: RGD NAD or NADH binding Ref.2 Inferred from direct assay. Source: RGD steroid binding Ref.2 Inferred from physical interaction. Source: RGD steroid delta-isomerase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 373	372	3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1		PRO_0000087787
Regions
	Transmembrane	288 – 308	21	Potential
Sites
	Active site	155	1	Proton acceptor By similarity
	Binding site	159	1	NAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	P22071-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 1BB058B5171C2F24	FASTA	373	42,037
	10 20 30 40 50 60 MPGWSCLVTG AGGFVGQRII RMLVQEKELQ EVRALDKVFR PETKEEFSKL QTKAKVTMLE 70 80 90 100 110 120 GDILDAQYLR RACQGISVVI HTAAVIDVSH VLPRQTILDV NLKGTQNILE ACVEASVPAF 130 140 150 160 170 180 IYCSTVDVAG PNSYKKIILN GHEEEHHEST WSDAYPYSKR MAEKAVLAAN GSILKNGGTL 190 200 210 220 230 240 HTCALRPMYI YGERSPFLSV MILAALKNKG ILNVTGKFSI ANPVYVGNVA WAHILAARGL 250 260 270 280 290 300 RDPKKSQNVQ GQFYYISDDT PHQSYDDLNC TLSKEWGLRL DSSWSLPLPL LYWLAFLLET 310 320 330 340 350 360 VSFLLRPFYN YRPPFNCHLV TLSNSKFTFS YKKAQRDLGY VPLVSWEEAK QKTSEWIGTL 370 VEQHRETLDT KSQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of rat 3 beta-hydroxysteroid dehydrogenase/delta 5-delta 4 isomerase cDNAs and differential tissue-specific expression of the corresponding mRNAs in steroidogenic and peripheral tissues." Zhao H.-F., Labrie C., Simard J., de Launoit Y., Trudel C., Martel C., Rheaume E., Dupont E., Luu-The V., Pelletier G., Labrie F. J. Biol. Chem. 266:583-593(1991) [PubMed: 1985917] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"3 beta-hydroxysteroid dehydrogenase/delta 5-->4-isomerase expression in rat and characterization of the testis isoform." Lorence M.C., Naville D., Graham-Lorence S.E., Mack S.O., Murry B.A., Trant J.M., Mason J.I. Mol. Cell. Endocrinol. 80:21-31(1991) [PubMed: 1955079] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Testis.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Ovary.
[4]	Si J.L., Li J., Xu X.Q. Submitted (APR-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 227-358. Strain: Wistar.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M38178 mRNA. Translation: AAA63474.1. BC086578 mRNA. Translation: AAH86578.1. DQ515797 mRNA. Translation: ABF70960.1.
IPI	IPI00362470.
PIR	DERTH1. A39051.
RefSeq	NP_001007720.3.
UniGene	Rn.128814
3D structure databases
ModBase	Search...
Proteomic databases
PRIDE	P22071.
Genome annotation databases
Ensembl	ENSRNOG00000019454. Rattus norvegicus. [Contig view]
GeneID	360348.
KEGG	rno:360348.
Organism-specific databases
RGD	1308676. Hsd3b1.
Phylogenomic databases
HOVERGEN	P22071.
OMA	P22071. LATCAIR.
Enzyme and pathway databases
BRENDA	1.1.1.145. 248. 5.3.3.1. 248.
Gene expression databases
ArrayExpress	P22071.
GermOnline	ENSRNOG00000019454. Rattus norvegicus.
Family and domain databases
InterPro	IPR002225. 3Beta_OHSteriod_DH/Estase. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF01073. 3Beta_HSD. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	672781.

Entry information

Entry name

3BHS1_RAT

Accession

Primary (citable) accession number: P22071
Secondary accession number(s): Q19P43

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1991
Last sequence update:	January 23, 2007
Last modified:	June 16, 2009
This is version 79 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)