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Protein

Protein P-30

Gene
N/A
Organism
Lithobates pipiens (Northern leopard frog) (Rana pipiens)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Basic protein with antiproliferative/cytotoxic activity against several tumor cell lines in vitro, as well as antitumor in vivo. It exhibits a ribonuclease-like activity against high molecular weight ribosomal RNA.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei10 – 101Proton acceptor
Active sitei97 – 971Proton donor

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Enzyme and pathway databases

BRENDAi3.1.27.5. 5287.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein P-30 (EC:3.1.27.-)
Alternative name(s):
Onconase
OrganismiLithobates pipiens (Northern leopard frog) (Rana pipiens)
Taxonomic identifieri8404 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraNeobatrachiaRanoideaRanidaeRana

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 104104Protein P-30PRO_0000057174Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11Pyrrolidone carboxylic acid
Disulfide bondi19 ↔ 68
Disulfide bondi30 ↔ 75
Disulfide bondi48 ↔ 90
Disulfide bondi87 ↔ 104

Keywords - PTMi

Disulfide bond, Pyrrolidone carboxylic acid

Expressioni

Developmental stagei

Early embryos (up to four blastomere stage).

Structurei

Secondary structure

1
104
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi3 – 108Combined sources
Beta strandi11 – 166Combined sources
Helixi19 – 224Combined sources
Beta strandi23 – 253Combined sources
Turni26 – 305Combined sources
Beta strandi32 – 398Combined sources
Helixi41 – 455Combined sources
Helixi46 – 483Combined sources
Beta strandi55 – 584Combined sources
Beta strandi63 – 708Combined sources
Beta strandi77 – 848Combined sources
Beta strandi86 – 916Combined sources
Beta strandi94 – 1029Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1ONCX-ray1.70A2-104[»]
1PU3NMR-A1-104[»]
1YV4X-ray1.51A1-104[»]
1YV6X-ray1.78A1-104[»]
1YV7X-ray1.90A1-102[»]
2GMKX-ray1.65A1-104[»]
2I5SX-ray1.90X1-104[»]
2KB6NMR-A1-104[»]
2LT5NMR-A2-104[»]
3FD7X-ray1.53A/B1-104[»]
3HG6X-ray1.70A2-104[»]
3PHNX-ray1.46A2-104[»]
3SNFX-ray1.10A1-104[»]
3U00X-ray1.65A2-104[»]
3U01X-ray1.12A2-104[»]
ProteinModelPortaliP22069.
SMRiP22069. Positions 1-104.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP22069.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni31 – 355Substrate binding

Sequence similaritiesi

Belongs to the pancreatic ribonuclease family.Curated

Phylogenomic databases

HOVERGENiHBG008396.

Family and domain databases

Gene3Di3.10.130.10. 1 hit.
InterProiIPR001427. RNaseA.
IPR023411. RNaseA_AS.
IPR023412. RNaseA_domain.
[Graphical view]
PfamiPF00074. RnaseA. 1 hit.
[Graphical view]
ProDomiPD000535. RNaseA. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00092. RNAse_Pc. 1 hit.
[Graphical view]
SUPFAMiSSF54076. SSF54076. 1 hit.
PROSITEiPS00127. RNASE_PANCREATIC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P22069-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
QDWLTFQKKH ITNTRDVDCD NIMSTNLFHC KDKNTFIYSR PEPVKAICKG
60 70 80 90 100
IIASKNVLTT SEFYLSDCNV TSRPCKYKLK KSTNKFCVTC ENQAPVHFVG

VGSC
Length:104
Mass (Da):11,845
Last modified:February 1, 1994 - v2
Checksum:i22A753C2F9E566B4
GO

Cross-referencesi

Web resourcesi

Functional Glycomics Gateway - Glycan Binding

Onconase

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1ONCX-ray1.70A2-104[»]
1PU3NMR-A1-104[»]
1YV4X-ray1.51A1-104[»]
1YV6X-ray1.78A1-104[»]
1YV7X-ray1.90A1-102[»]
2GMKX-ray1.65A1-104[»]
2I5SX-ray1.90X1-104[»]
2KB6NMR-A1-104[»]
2LT5NMR-A2-104[»]
3FD7X-ray1.53A/B1-104[»]
3HG6X-ray1.70A2-104[»]
3PHNX-ray1.46A2-104[»]
3SNFX-ray1.10A1-104[»]
3U00X-ray1.65A2-104[»]
3U01X-ray1.12A2-104[»]
ProteinModelPortaliP22069.
SMRiP22069. Positions 1-104.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

HOVERGENiHBG008396.

Enzyme and pathway databases

BRENDAi3.1.27.5. 5287.

Miscellaneous databases

EvolutionaryTraceiP22069.

Family and domain databases

Gene3Di3.10.130.10. 1 hit.
InterProiIPR001427. RNaseA.
IPR023411. RNaseA_AS.
IPR023412. RNaseA_domain.
[Graphical view]
PfamiPF00074. RnaseA. 1 hit.
[Graphical view]
ProDomiPD000535. RNaseA. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00092. RNAse_Pc. 1 hit.
[Graphical view]
SUPFAMiSSF54076. SSF54076. 1 hit.
PROSITEiPS00127. RNASE_PANCREATIC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRNP30_LITPI
AccessioniPrimary (citable) accession number: P22069
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: February 1, 1994
Last modified: October 14, 2015
This is version 91 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.