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Protein

Protein-L-isoaspartate(D-aspartate) O-methyltransferase

Gene

Pcmt1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the methyl esterification of L-isoaspartyl and D-aspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins. Acts on EIF4EBP2, microtubule-associated protein 2, calreticulin, clathrin light chains a and b, Ubiquitin carboxyl-terminal hydrolase isozyme L1, phosphatidylethanolamine-binding protein 1, stathmin, beta-synuclein and alpha-synuclein.By similarity

Catalytic activityi

S-adenosyl-L-methionine + protein L-isoaspartate = S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei60 – 601By similarity

GO - Molecular functioni

  • protein-L-isoaspartate (D-aspartate) O-methyltransferase activity Source: RGD
  • S-adenosylmethionine-dependent methyltransferase activity Source: RGD

GO - Biological processi

  • aging Source: RGD
  • cellular response to hypoxia Source: RGD
  • negative regulation of cardiac muscle cell apoptotic process Source: RGD
  • protein methylation Source: RGD
  • response to ethanol Source: RGD
  • S-adenosylhomocysteine metabolic process Source: RGD
  • S-adenosylmethionine metabolic process Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Ligandi

S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
Protein-L-isoaspartate(D-aspartate) O-methyltransferase (EC:2.1.1.77)
Short name:
PIMT
Alternative name(s):
L-isoaspartyl protein carboxyl methyltransferase
Protein L-isoaspartyl/D-aspartyl methyltransferase
Protein-beta-aspartate methyltransferase
Gene namesi
Name:Pcmt1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi3268. Pcmt1.

Subcellular locationi

GO - Cellular componenti

  • basolateral plasma membrane Source: RGD
  • brush border membrane Source: RGD
  • cytoplasm Source: RGD
  • cytosol Source: RGD
  • extracellular space Source: RGD
  • neuron projection Source: RGD
  • perikaryon Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 227226Protein-L-isoaspartate(D-aspartate) O-methyltransferasePRO_0000111878Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiP22062.
PRIDEiP22062.

PTM databases

iPTMnetiP22062.
PhosphoSiteiP22062.

Expressioni

Gene expression databases

BgeeiENSRNOG00000014330.
GenevisibleiP22062. RN.

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

IntActiP22062. 1 interaction.
MINTiMINT-4589251.
STRINGi10116.ENSRNOP00000019623.

Structurei

3D structure databases

ProteinModelPortaliP22062.
SMRiP22062. Positions 3-226.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1661. Eukaryota.
COG2518. LUCA.
HOGENOMiHOG000257189.
HOVERGENiHBG004483.
InParanoidiP22062.
KOiK00573.
OrthoDBiEOG091G0OTG.
PhylomeDBiP22062.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR000682. PCMT.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR11579. PTHR11579. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00080. pimt. 1 hit.
PROSITEiPS01279. PCMT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P22062-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAWKSGGASH SELIHNLRKN GIIKTDKVFE VMLATDRSHY AKSNPYMDSP
60 70 80 90 100
QSIGFQATIS APHMHAYALE LLFDQLHEGA KALDVGSGSG ILTACFARMV
110 120 130 140 150
GHSGKVIGID HIKELVDDSI TNVKKDDPML LSSGRVRLVV GDGRMGFAEE
160 170 180 190 200
APYDAIHVGA AAPVVPQALI DQLKPGGRLI LPVGPAGGNQ MLEQYDKLQD
210 220
GSVKMKPLMG VIYVPLTDKE KQWSRWK
Length:227
Mass (Da):24,641
Last modified:January 23, 2007 - v2
Checksum:i833498527365E24F
GO

Sequence cautioni

The sequence BAA02034 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti83 – 831L → P in AAA60742 (PubMed:2730663).Curated
Sequence conflicti83 – 831L → P in BAA02034 (PubMed:8263531).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M26686 mRNA. Translation: AAA60742.1.
D11475 mRNA. Translation: BAA02034.1. Different initiation.
BC088417 mRNA. Translation: AAH88417.1.
PIRiA32449.
RefSeqiNP_037205.2. NM_013073.3.
UniGeneiRn.86985.

Genome annotation databases

GeneIDi25604.
KEGGirno:25604.
UCSCiRGD:3268. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M26686 mRNA. Translation: AAA60742.1.
D11475 mRNA. Translation: BAA02034.1. Different initiation.
BC088417 mRNA. Translation: AAH88417.1.
PIRiA32449.
RefSeqiNP_037205.2. NM_013073.3.
UniGeneiRn.86985.

3D structure databases

ProteinModelPortaliP22062.
SMRiP22062. Positions 3-226.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP22062. 1 interaction.
MINTiMINT-4589251.
STRINGi10116.ENSRNOP00000019623.

PTM databases

iPTMnetiP22062.
PhosphoSiteiP22062.

Proteomic databases

PaxDbiP22062.
PRIDEiP22062.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi25604.
KEGGirno:25604.
UCSCiRGD:3268. rat.

Organism-specific databases

CTDi5110.
RGDi3268. Pcmt1.

Phylogenomic databases

eggNOGiKOG1661. Eukaryota.
COG2518. LUCA.
HOGENOMiHOG000257189.
HOVERGENiHBG004483.
InParanoidiP22062.
KOiK00573.
OrthoDBiEOG091G0OTG.
PhylomeDBiP22062.

Miscellaneous databases

PROiP22062.

Gene expression databases

BgeeiENSRNOG00000014330.
GenevisibleiP22062. RN.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR000682. PCMT.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR11579. PTHR11579. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00080. pimt. 1 hit.
PROSITEiPS01279. PCMT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPIMT_RAT
AccessioniPrimary (citable) accession number: P22062
Secondary accession number(s): Q5M7V6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: January 23, 2007
Last modified: September 7, 2016
This is version 117 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.