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Protein

Protein-L-isoaspartate(D-aspartate) O-methyltransferase

Gene

Pcmt1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the methyl esterification of L-isoaspartyl and D-aspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins. Acts on EIF4EBP2, microtubule-associated protein 2, calreticulin, clathrin light chains a and b, Ubiquitin carboxyl-terminal hydrolase isozyme L1, phosphatidylethanolamine-binding protein 1, stathmin, beta-synuclein and alpha-synuclein.By similarity

Catalytic activityi

S-adenosyl-L-methionine + protein L-isoaspartate = S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei60By similarity1

GO - Molecular functioni

  • protein-L-isoaspartate (D-aspartate) O-methyltransferase activity Source: RGD
  • S-adenosylmethionine-dependent methyltransferase activity Source: RGD

GO - Biological processi

  • aging Source: RGD
  • cellular response to hypoxia Source: RGD
  • negative regulation of cardiac muscle cell apoptotic process Source: RGD
  • protein methylation Source: RGD
  • response to ethanol Source: RGD
  • S-adenosylhomocysteine metabolic process Source: RGD
  • S-adenosylmethionine metabolic process Source: RGD

Keywordsi

Molecular functionMethyltransferase, Transferase
LigandS-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
Protein-L-isoaspartate(D-aspartate) O-methyltransferase (EC:2.1.1.77)
Short name:
PIMT
Alternative name(s):
L-isoaspartyl protein carboxyl methyltransferase
Protein L-isoaspartyl/D-aspartyl methyltransferase
Protein-beta-aspartate methyltransferase
Gene namesi
Name:Pcmt1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi3268 Pcmt1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001118782 – 227Protein-L-isoaspartate(D-aspartate) O-methyltransferaseAdd BLAST226

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiP22062
PRIDEiP22062

PTM databases

iPTMnetiP22062
PhosphoSitePlusiP22062

Expressioni

Gene expression databases

GenevisibleiP22062 RN

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

IntActiP22062, 1 interactor
MINTiP22062
STRINGi10116.ENSRNOP00000019623

Structurei

3D structure databases

ProteinModelPortaliP22062
SMRiP22062
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1661 Eukaryota
COG2518 LUCA
HOGENOMiHOG000257189
HOVERGENiHBG004483
InParanoidiP22062
KOiK00573
OrthoDBiEOG091G0OTG
PhylomeDBiP22062

Family and domain databases

InterProiView protein in InterPro
IPR000682 PCMT
IPR029063 SAM-dependent_MTases
PANTHERiPTHR11579 PTHR11579, 1 hit
SUPFAMiSSF53335 SSF53335, 1 hit
TIGRFAMsiTIGR00080 pimt, 1 hit
PROSITEiView protein in PROSITE
PS01279 PCMT, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P22062-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAWKSGGASH SELIHNLRKN GIIKTDKVFE VMLATDRSHY AKSNPYMDSP
60 70 80 90 100
QSIGFQATIS APHMHAYALE LLFDQLHEGA KALDVGSGSG ILTACFARMV
110 120 130 140 150
GHSGKVIGID HIKELVDDSI TNVKKDDPML LSSGRVRLVV GDGRMGFAEE
160 170 180 190 200
APYDAIHVGA AAPVVPQALI DQLKPGGRLI LPVGPAGGNQ MLEQYDKLQD
210 220
GSVKMKPLMG VIYVPLTDKE KQWSRWK
Length:227
Mass (Da):24,641
Last modified:January 23, 2007 - v2
Checksum:i833498527365E24F
GO

Sequence cautioni

The sequence BAA02034 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti83L → P in AAA60742 (PubMed:2730663).Curated1
Sequence conflicti83L → P in BAA02034 (PubMed:8263531).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M26686 mRNA Translation: AAA60742.1
D11475 mRNA Translation: BAA02034.1 Different initiation.
BC088417 mRNA Translation: AAH88417.1
PIRiA32449
RefSeqiNP_037205.2, NM_013073.3
UniGeneiRn.86985

Genome annotation databases

GeneIDi25604
KEGGirno:25604
UCSCiRGD:3268 rat

Similar proteinsi

Entry informationi

Entry nameiPIMT_RAT
AccessioniPrimary (citable) accession number: P22062
Secondary accession number(s): Q5M7V6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: January 23, 2007
Last modified: May 23, 2018
This is version 126 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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