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Protein

Protein-L-isoaspartate(D-aspartate) O-methyltransferase

Gene

PCMT1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the methyl esterification of L-isoaspartyl and D-aspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins. Acts on EIF4EBP2, microtubule-associated protein 2, calreticulin, clathrin light chains a and b, Ubiquitin carboxyl-terminal hydrolase isozyme L1, phosphatidylethanolamine-binding protein 1, stathmin, beta-synuclein and alpha-synuclein.By similarity

Catalytic activityi

S-adenosyl-L-methionine + protein L-isoaspartate = S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei601

GO - Molecular functioni

  • cadherin binding Source: BHF-UCL
  • protein-L-isoaspartate (D-aspartate) O-methyltransferase activity Source: UniProtKB

GO - Biological processi

  • protein methylation Source: ProtInc
  • protein repair Source: UniProtKB

Keywordsi

Molecular functionMethyltransferase, Transferase
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciMetaCyc:HS04385-MONOMER
BRENDAi2.1.1.77 2681
ReactomeiR-HSA-5676934 Protein repair

Names & Taxonomyi

Protein namesi
Recommended name:
Protein-L-isoaspartate(D-aspartate) O-methyltransferase (EC:2.1.1.77)
Short name:
PIMT
Alternative name(s):
L-isoaspartyl protein carboxyl methyltransferase
Protein L-isoaspartyl/D-aspartyl methyltransferase
Protein-beta-aspartate methyltransferase
Gene namesi
Name:PCMT1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 6

Organism-specific databases

EuPathDBiHostDB:ENSG00000120265.16
HGNCiHGNC:8728 PCMT1
MIMi176851 gene
neXtProtiNX_P22061

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

DisGeNETi5110

Chemistry databases

ChEMBLiCHEMBL4240
DrugBankiDB01752 S-Adenosyl-L-Homocysteine

Polymorphism and mutation databases

BioMutaiPCMT1
DMDMi317373537

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001118752 – 227Protein-L-isoaspartate(D-aspartate) O-methyltransferaseAdd BLAST226

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanine1 Publication1
Disulfide bondi43 ↔ 95By similarity

Keywords - PTMi

Acetylation, Disulfide bond

Proteomic databases

EPDiP22061
MaxQBiP22061
PaxDbiP22061
PeptideAtlasiP22061
PRIDEiP22061

2D gel databases

OGPiP22061
REPRODUCTION-2DPAGEIPI00411680
UCD-2DPAGEP22061

PTM databases

iPTMnetiP22061
PhosphoSitePlusiP22061

Expressioni

Gene expression databases

BgeeiENSG00000120265
ExpressionAtlasiP22061 baseline and differential
GenevisibleiP22061 HS

Organism-specific databases

HPAiHPA003239

Interactioni

Subunit structurei

Monomer.

Binary interactionsi

Show more details

GO - Molecular functioni

Protein-protein interaction databases

BioGridi111141, 112 interactors
IntActiP22061, 26 interactors
MINTiP22061
STRINGi9606.ENSP00000356348

Chemistry databases

BindingDBiP22061

Structurei

Secondary structure

1227
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi10 – 19Combined sources10
Helixi26 – 33Combined sources8
Helixi37 – 39Combined sources3
Beta strandi47 – 49Combined sources3
Beta strandi51 – 54Combined sources4
Beta strandi57 – 59Combined sources3
Helixi62 – 71Combined sources10
Turni72 – 75Combined sources4
Beta strandi81 – 85Combined sources5
Helixi91 – 100Combined sources10
Turni101 – 103Combined sources3
Beta strandi105 – 111Combined sources7
Helixi113 – 126Combined sources14
Helixi129 – 132Combined sources4
Beta strandi134 – 141Combined sources8
Helixi143 – 145Combined sources3
Helixi148 – 150Combined sources3
Beta strandi153 – 158Combined sources6
Beta strandi160 – 164Combined sources5
Helixi167 – 171Combined sources5
Beta strandi173 – 184Combined sources12
Beta strandi190 – 197Combined sources8
Beta strandi203 – 211Combined sources9
Helixi219 – 222Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1I1NX-ray1.50A2-227[»]
1KR5X-ray2.10A2-227[»]
ProteinModelPortaliP22061
SMRiP22061
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP22061

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1661 Eukaryota
COG2518 LUCA
HOVERGENiHBG004483
InParanoidiP22061
KOiK00573
PhylomeDBiP22061
TreeFamiTF314431

Family and domain databases

InterProiView protein in InterPro
IPR000682 PCMT
IPR029063 SAM-dependent_MTases
PANTHERiPTHR11579 PTHR11579, 1 hit
SUPFAMiSSF53335 SSF53335, 1 hit
TIGRFAMsiTIGR00080 pimt, 1 hit
PROSITEiView protein in PROSITE
PS01279 PCMT, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P22061-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAWKSGGASH SELIHNLRKN GIIKTDKVFE VMLATDRSHY AKCNPYMDSP
60 70 80 90 100
QSIGFQATIS APHMHAYALE LLFDQLHEGA KALDVGSGSG ILTACFARMV
110 120 130 140 150
GCTGKVIGID HIKELVDDSV NNVRKDDPTL LSSGRVQLVV GDGRMGYAEE
160 170 180 190 200
APYDAIHVGA AAPVVPQALI DQLKPGGRLI LPVGPAGGNQ MLEQYDKLQD
210 220
GSIKMKPLMG VIYVPLTDKE KQWSRWK
Length:227
Mass (Da):24,636
Last modified:January 11, 2011 - v4
Checksum:i4EB1122379C8040A
GO
Isoform 2 (identifier: P22061-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     226-227: WK → DEL

Show »
Length:228
Mass (Da):24,679
Checksum:i69E688C22379C804
GO

Sequence cautioni

The sequence EAW47786 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti19K → G AA sequence (PubMed:3167043).Curated1
Sequence conflicti23I → L AA sequence (PubMed:2684970).Curated1
Sequence conflicti60S → A AA sequence (PubMed:3167043).Curated1
Sequence conflicti102C → Q AA sequence (PubMed:2684970).Curated1
Sequence conflicti168A → P AA sequence (PubMed:3167043).Curated1
Sequence conflicti206K → R in AAA90933 (PubMed:1339271).Curated1

Polymorphismi

The allele frequencies for the polymorphism at codon 120 differ between ethnic groups; in the Caucasian population Ile-120 is present at a frequency of 0.45, while it is found at a frequency of 0.88 and 0.81 in the Asian and the African populations respectively. Val-120 is found at a frequency of 0.55 in the Caucasians, 0.12 and 0.19 in the Asian and African populations respectively. The Ile-120 variant has higher specific activity and thermostability than the Val-120 variant. The Val-120 variant has a higher affinity for protein substrates.

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_006173120V → ICombined sources5 PublicationsCorresponds to variant dbSNP:rs4816Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_004716226 – 227WK → DEL in isoform 2. 4 Publications2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M93008 mRNA Translation: AAA90934.1
M93009 mRNA Translation: AAA90933.1
D25545 mRNA Translation: BAA05028.1
D25546 mRNA Translation: BAA05029.1
D25547 mRNA Translation: BAA05030.1
D13892 mRNA Translation: BAA02991.1
AK289724 mRNA Translation: BAF82413.1
AL355312 Genomic DNA No translation available.
CH471051 Genomic DNA Translation: EAW47786.1 Sequence problems.
BC007501 mRNA Translation: AAH07501.1
BC008748 mRNA Translation: AAH08748.1
U49740 Genomic DNA Translation: AAB38386.1
S73902 Genomic DNA Translation: AAC60639.2
S73903 Genomic DNA Translation: AAC60640.1
S73905 Genomic DNA Translation: AAC60641.2
PIRiA34489
JH0624
RefSeqiNP_001238978.1, NM_001252049.1
NP_001238982.1, NM_001252053.1
NP_005380.2, NM_005389.2
UniGeneiHs.279257

Genome annotation databases

EnsembliENST00000367380; ENSP00000356350; ENSG00000120265
GeneIDi5110
KEGGihsa:5110
UCSCiuc011eeg.3 human [P22061-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiPIMT_HUMAN
AccessioniPrimary (citable) accession number: P22061
Secondary accession number(s): A8K109
, J3KP72, Q14661, Q16556, Q5VYC1, Q5VYC2, Q93061, Q96II9, Q99625, Q9BQV7, Q9BQV8, Q9NP03
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: January 11, 2011
Last modified: May 23, 2018
This is version 191 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

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