Reviewed, UniProtKB/Swiss-Prot P22045 (P100_LEIMA)

Names and origin

Protein names

Recommended name:
    Probable reductase
    EC=1.1.-.-

Gene names

Name:

P100/11E

Organism

Leishmania major

Taxonomic identifier

5664 [NCBI]

Taxonomic lineage

Eukaryota › Euglenozoa › Kinetoplastida › Trypanosomatidae › Leishmania

Protein attributes

Sequence length	284 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Developmental stage	P110/11E abundance is markedly elevated in promastigotes relative to amastigotes.
Sequence similarities	Belongs to the aldo/keto reductase family.

Ontologies

Keywords
Ligand	NADP
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	oxidoreductase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 284	284	Probable reductase		PRO_0000124653
Regions
	Nucleotide binding	198 – 250	53	NADP By similarity
Sites
	Active site	54	1	Proton donor By similarity
	Binding site	112	1	Substrate By similarity
	Site	79	1	Lowers pKa of active site Tyr By similarity

Sequences

	Sequence		Length	Mass (Da)
	P22045-1 [UniParc]. Last modified November 1, 1995. Version 2. Checksum: 82FF5B17F7EDC6CC	FASTA	284	31,836
	10 20 30 40 50 60 MAGVDKAMVT LSNGVKMPQF GLGVWQSPAG EVTENAVNWA LCAGYRHIDT AAIYKNEESV 70 80 90 100 110 120 GAGLRASGVP REDVFITTKL WNTEQGYEST LAAFEESRQK LGVDYIDLYL IHWPRGKDIL 130 140 150 160 170 180 SKEGKKYLDS WRAFEQLYKE KKVRAIGVSN FHIHHLEDVL AMCTVTPMVN QVELHPLNNQ 190 200 210 220 230 240 ADLRAFCDAK QIKVEAWSPL GQGKLLSNPI LSAIGAKYNK TAAQVILRWN IQKNLITIPK 250 260 270 280 SVHRERIEEN ADIFDFELGA EDVMSIDALN TNSRYGPDPD EAQF

References

[1]	"The developmentally regulated P100/11E gene of Leishmania major shows homology to a superfamily of reductase genes." Samaras N., Spithill T.W. J. Biol. Chem. 264:4251-4254(1989) [PubMed: 2918000] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	J04483 mRNA. Translation: AAA57350.1.
PIR	A32950.
3D structure databases
HSSP	HSSP built from PDB template 1HW6 based on UniProtKB P06632.
SMR	P22045. Positions 9-280.
ModBase	Search...
Family and domain databases
InterPro	IPR001395. Aldo/ket_red. IPR018170. Aldo/ket_reductase_CS. [Graphical view]
Gene3D	G3DSA:3.20.20.100. Aldo/ket_red. 1 hit.
PANTHER	PTHR11732. Aldo/ket_red. 1 hit.
Pfam	PF00248. Aldo_ket_red. 1 hit. [Graphical view]
ProDom	PD000288. Aldo/ket_red. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00798. ALDOKETO_REDUCTASE_1. 1 hit. PS00062. ALDOKETO_REDUCTASE_2. 1 hit. PS00063. ALDOKETO_REDUCTASE_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

P100_LEIMA

Accession

Primary (citable) accession number: P22045

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1991
Last sequence update:	November 1, 1995
Last modified:	June 16, 2009
This is version 47 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)