Reviewed, UniProtKB/Swiss-Prot P22035 (BAS1_YEAST)

Names and origin

Protein names

Recommended name:
    Myb-like DNA-binding protein BAS1

Gene names

Name:	BAS1
Ordered Locus Names:	YKR099W

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	811 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Activates HIS4 transcription only in combination with PHO2/BAS2. BAS1 is also involved in the regulation of the purine biosynthesis pathway. Ref.4
Subunit structure	Monomer.
Subcellular location	Nucleus.
Miscellaneous	Present with 861 molecules/cell in log phase SD medium. Ref.5
Sequence similarities	Contains 2 HTH myb-type DNA-binding domains. Contains 1 Myb-like domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Domain	Repeat
Ligand	DNA-binding
Molecular function	Activator
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	histidine biosynthetic process Inferred from direct assay. Source: SGD purine base biosynthetic process Inferred from direct assay. Source: SGD reciprocal meiotic recombination Inferred from direct assay. Source: SGD regulation of transcription Inferred from electronic annotation. Source: UniProtKB-KW transcription from RNA polymerase II promoter Inferred from direct assay. Source: SGD
Cellular component	nucleus Inferred from direct assay. Source: SGD
Molecular function	protein binding Inferred from physical interaction. Source: IntAct sequence-specific DNA binding Inferred from direct assay. Source: SGD specific RNA polymerase II transcription factor activity Inferred from direct assay. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
SHM2	P37291	1	EBI-3428,EBI-7744

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 811	811	Myb-like DNA-binding protein BAS1		PRO_0000197085
Regions
	Domain	34 – 110	77	Myb-like
	Domain	111 – 165	55	HTH myb-type 1
	Domain	166 – 218	53	HTH myb-type 2
	DNA binding	138 – 161	24	H-T-H motif By similarity
	DNA binding	191 – 214	24	H-T-H motif By similarity
Amino acid modifications
	Modified residue	317	1	Phosphoserine Ref.6
	Modified residue	557	1	Phosphoserine Ref.6
	Modified residue	656	1	Phosphoserine Ref.6

Sequences

	Sequence		Length	Mass (Da)
	P22035-1 [UniParc]. Last modified August 1, 1991. Version 1. Checksum: E874E72A3B7BDDE5	FASTA	811	89,604
	10 20 30 40 50 60 MSNISTKDIR KSKPKRGSGF DLLEVTESLG YQTHRKNGRN SWSKDDDNML RSLVNESAKE 70 80 90 100 110 120 LGYENGLEDV KTIQQSNHLS KCIAWDVLAT RFKHTVRTSK DVRKRWTGSL DPNLKKGKWT 130 140 150 160 170 180 QEEDEQLLKA YEEHGPHWLS ISMDIPGRTE DQCAKRYIEV LGPGSKGRLR EWTLEEDLNL 190 200 210 220 230 240 ISKVKAYGTK WRKISSEMEF RPSLTCRNRW RKIITMVVRG QASEVITKAI KENKNIDMTD 250 260 270 280 290 300 GKLRQHPIAD SDIRSDSTPN KEEQLQLSQQ NNPSLIKQDI LNVKENESSK LPRLKDNDGP 310 320 330 340 350 360 ILNDSKPQAL PPLKEISAPP PIRMTQVGQT HTSGSIRSKV SLPIEGLSQM NKQSPGGISD 370 380 390 400 410 420 SPQTSLPPAF NPASLDEHMM NSNSISDSPK HAYSTVKTRE PNSSSTQWKF TLKDGQGLSI 430 440 450 460 470 480 SNGTIDSTKL VKELVDQAKK YSLKISIHQH IHNHYVTSTD HPVSSNTGLS NIGNINGNPL 490 500 510 520 530 540 LMDSFPHMGR QLGNGLPGLN SNSDTFNPEY RTSLDNMDSD FLSRTPNYNA FSLEATSHNP 550 560 570 580 590 600 ADNANELGSQ SNRETNSPSV FYPQANTLIP TNSTATNNEI IQGNVSANSM SPNFNGTNGK 610 620 630 640 650 660 APSSTASYTT SGSEMPPDVG PNRIAHFNYL PPTIRPHLGS SDATRGADLN KLLNPSPNSV 670 680 690 700 710 720 RSNGSKTKKK EKRKSESSQH HSSSSVTTNK FNHIDQSEIS RTTSRSDTPL RDEDGLDFWE 730 740 750 760 770 780 TLRSLATTNP NPPVEKSAEN DGAKPQVVHQ GIGSHTEDSS LGSHSGGYDF FNELLDKKAD 790 800 810 TLHNEAKKTS EHDMTSGGST DNGSVLPLNP S

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"BAS1 has a Myb motif and activates HIS4 transcription only in combination with BAS2." Tice-Baldwin K., Fink G.R., Arndt K.T. Science 246:931-935(1989) [PubMed: 2683089] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete DNA sequence of yeast chromosome XI." Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W. Nature 369:371-378(1994) [PubMed: 8196765] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	"DNA-binding domain and recognition sequence of the yeast BAS1 protein, a divergent member of the Myb family of transcription factors." Hoevring P.I., Bostad A., Ording E., Myrset A.H., Gabrielsen O.S. J. Biol. Chem. 269:17663-17669(1994) [PubMed: 8021277] [Abstract] Cited for: DNA-BINDING REGION.
[4]	"Role of the myb-like protein bas1p in Saccharomyces cerevisiae: a proteome analysis." Denis V., Boucherie H., Monribot C., Daignan-Fornier B. Mol. Microbiol. 30:557-566(1998) [PubMed: 9822821] [Abstract] Cited for: FUNCTION.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-317; SER-557 AND SER-656, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M58057 Genomic DNA. Translation: AAB04030.1. Z28324 Genomic DNA. Translation: CAA82179.1.
PIR	A40083.
RefSeq	NP_013025.1.
3D structure databases
SMR	P22035. Positions 39-161, 111-215.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-142N.
IntAct	P22035. 5 interactions.
STRING	P22035.
Genome annotation databases
Ensembl	YKR099W; YKR099W; YKR099W; Saccharomyces cerevisiae. [Genome view]
GeneID	853974.
KEGG	sce:YKR099W.
NMPDR	fig|4932.3.peg.4013.
Organism-specific databases
CYGD	YKR099w.
SGD	S000001807. BAS1.
Phylogenomic databases
eggNOG	fuNOG05261.
HOGENOM	HBG202395.
OMA	ISIHQHI.
OrthoDB	EOG9WDFV5.
PhylomeDB	P22035.
Gene expression databases
ArrayExpress	P22035.
Genevestigator	P22035.
GermOnline	YKR099W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR009057. Homeodomain-like. IPR012287. Homeodomain-rel. IPR017930. HTH_Myb-type_DNA-bd. IPR017877. MYB-like. IPR014778. Myb_DNA-bd. IPR015495. Myb_trans_fac. IPR001005. SANT_DNA-bd. [Graphical view]
Gene3D	G3DSA:1.10.10.60. Homeodomain-rel. 2 hits.
PANTHER	PTHR10641. Myb_transfac. 1 hit.
Pfam	PF00249. Myb_DNA-binding. 2 hits. [Graphical view]
SMART	SM00717. SANT. 3 hits. [Graphical view]
PROSITE	PS51294. HTH_MYB. 2 hits. PS50090. MYB_LIKE. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	975425.

Entry information

Entry name

BAS1_YEAST

Accession

Primary (citable) accession number: P22035

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1991
Last sequence update:	August 1, 1991
Last modified:	January 19, 2010
This is version 93 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)