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Protein

Zinc metalloproteinase-disintegrin-like botrocetin

Gene
N/A
Organism
Bothrops jararaca (Jararaca) (Bothrops jajaraca)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Metalloproteinase that binds to von Willebrand factor (VWF) and induces its interaction with GPIb (GP1BA), resulting in platelet aggregation.

Miscellaneous

There are two distinct forms of the vWF-dependent platelet coagglutinin. The dimeric form (snaclec) is 34-fold more active than the metalloprotease botrocetin in promoting vWF binding to platelets (PubMed:1993206).1 Publication

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi6Calcium; via carbonyl oxygenBy similarity1
Metal bindingi9CalciumBy similarity1
Metal bindingi11Calcium; via carbonyl oxygenBy similarity1
Metal bindingi13CalciumBy similarity1
Metal bindingi16CalciumBy similarity1
Metal bindingi19CalciumBy similarity1

GO - Molecular functioni

GO - Biological processi

  • negative regulation of platelet aggregation in other organism Source: CACAO

Keywordsi

Molecular functionCell adhesion impairing toxin, Hemostasis impairing toxin, Hydrolase, Platelet aggregation activating toxin, Toxin
LigandCalcium, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Zinc metalloproteinase-disintegrin-like botrocetin (EC:3.4.24.-)
Alternative name(s):
Snake venom metalloproteinase
Short name:
SVMP
von Willebrand factor-dependent platelet coagglutinin
Cleaved into the following chain:
OrganismiBothrops jararaca (Jararaca) (Bothrops jajaraca)
Taxonomic identifieri8724 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiLepidosauriaSquamataBifurcataUnidentataEpisquamataToxicoferaSerpentesColubroideaViperidaeCrotalinaeBothrops

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_0000046696‹1 – ›25Zinc metalloproteinase-disintegrin-like botrocetinAdd BLAST›25
ChainiPRO_00004224241 – ›25Disintegrin-like botrocetinAdd BLAST›25

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi18 ↔ ?By similarity
Disulfide bondi20 ↔ ?By similarity

Keywords - PTMi

Disulfide bond

Expressioni

Tissue specificityi

Expressed by the venom gland.

Interactioni

Subunit structurei

Monomer. Botrocetin and vWF form a soluble complex.1 Publication

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini4 – ›25DisintegrinAdd BLAST›22

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi17 – 19D/ECD-tripeptide3

Sequence similaritiesi

Sequencei

Sequence statusi: Fragment.

Sequence processingi: The displayed sequence is further processed into a mature form.

P22028-1 [UniParc]FASTAAdd to basket

« Hide

        10         20 
IISPPVCGNE LLEEGEECDC GTPEN
Length:25
Mass (Da):2,648
Last modified:May 29, 2013 - v2
Checksum:iD25D9031A7119AF8
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Non-terminal residuei11
Sequence uncertaintyi20Assigned by comparison with orthologs1
Non-terminal residuei251

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti2I → V. 1

Similar proteinsi

Entry informationi

Entry nameiVM3B_BOTJA
AccessioniPrimary (citable) accession number: P22028
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: May 29, 2013
Last modified: November 22, 2017
This is version 68 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
Annotation programAnimal Toxin Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families