P22023 (KRE5_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Killer toxin-resistance protein 5

Gene names

Name:	KRE5
Ordered Locus Names:	YOR336W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	1365 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for (1->6)-beta-D-glucan synthesis and normal cell growth.
Subcellular location	Endoplasmic reticulum lumen.
Miscellaneous	Present with 815 molecules/cell in log phase SD medium.
Sequence similarities	To D.melanogaster UGGG.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation
Cellular component	Endoplasmic reticulum
Domain	Signal
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	(1->6)-beta-D-glucan biosynthetic process Traceable author statement PubMed 8550469. Source: SGD protein glycosylation Inferred from electronic annotation. Source: InterPro
Cellular_component	endoplasmic reticulum Traceable author statement PubMed 10601196. Source: SGD endoplasmic reticulum lumen Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	UDP-glucose:glycoprotein glucosyltransferase activity Inferred from direct assay PubMed 9611196. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 17	17	Potential
	Chain	18 – 1365	1348	Killer toxin-resistance protein 5		PRO_0000021563
Regions
	Motif	1362 – 1365	4	Prevents secretion from ER
Amino acid modifications
	Glycosylation	115	1	N-linked (GlcNAc...) Potential
	Glycosylation	228	1	N-linked (GlcNAc...) Potential
	Glycosylation	293	1	N-linked (GlcNAc...) Potential
	Glycosylation	457	1	N-linked (GlcNAc...) Potential
	Glycosylation	519	1	N-linked (GlcNAc...) Potential
	Glycosylation	523	1	N-linked (GlcNAc...) Potential
	Glycosylation	644	1	N-linked (GlcNAc...) Potential
	Glycosylation	870	1	N-linked (GlcNAc...) Potential
	Glycosylation	1091	1	N-linked (GlcNAc...) Potential
	Glycosylation	1150	1	N-linked (GlcNAc...) Potential
	Glycosylation	1195	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	582	1	Missing in AAA34725. Ref.1
	Sequence conflict	780 – 794	15	HLDQN…EHFEA → ILIKMKCQKQNISKAK in AAA34725. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P22023 [UniParc]. Last modified November 1, 1997. Version 2. Checksum: D0F5851175CC0333	FASTA	1,365	156,477
	10 20 30 40 50 60 MRLLALVLLL LCAPLRAWTY SLRYGIPESA QVWSILVHLL GDVDNQLLTN LYPLVTGLDD 70 80 90 100 110 120 EIDIQENLVA LTSNVLRERY DKEDVADLLE LYASLYPMGM IQHDISSNAE QDDANSSYFV 130 140 150 160 170 180 LNGNRYEKPD DVFYLKSKDL TIQQKVPDVD VIQPYDVVIG TNSEAPILIL YGCPTVIDSD 190 200 210 220 230 240 FEEFNRNLFM EAMNGEGKFR FIWRSTCSLD GKSVEYPLTH PLEITLQNGS RMSSIPQLKK 250 260 270 280 290 300 ILYTVPKEIL VGADNDDQLH DLEPEELREL DLRVTSLISE FYQYKKDITA TLNFTKSIVN 310 320 330 340 350 360 NFPLISKQLI KVSSVNKDII TSNEELNSKG FDYNMLGLYI NGQNWKITSL TPYNLLTALK 370 380 390 400 410 420 TEYQSLLKIT NLLQELEPSK CILDSKFLLN KFSQFSLGKL QNLQPIKMDL HTIPGFSESV 430 440 450 460 470 480 IYFNDIESDP QYDELVNSVQ AFFDKSKFGE LPEIKQNWSE IIFVIDFARL EDSEVKEALG 490 500 510 520 530 540 GLVRAVNVVS QGYPQRVGLL PFSSDSDKSV VNKIYELKNS TDNLTELKSF LETMLLADGL 550 560 570 580 590 600 SANAKHSKHI PVPDVFHLLD ELQIDETSII INGEIYPFRK NAWNYLIAKV IKKDTEFIRK 610 620 630 640 650 660 ELSNSSPKNK QISVRDLLHY KSANLRHNKY TPNYFADSVY SSVNNTALES VCSERIGYYT 670 680 690 700 710 720 KNEEYNLLHT ITLVDDFGSI HALKRLRNLL HTSFVGVRIR IIHVGDISDI WYQLRGSLSQ 730 740 750 760 770 780 KDPIGSINTF IDALKLKKVK SHTYKKSGLN QLGLHKWLPD IPLFELQKGS FIALNGRFIH 790 800 810 820 830 840 LDQNEVPETE HFEAIIKREA LRTIDSVFAL DLLFPGFSQE IINPDLIEMI SSILTRLFYQ 850 860 870 880 890 900 GTHIYNNGID YTTESSLPRM DLSEFFRPNN LTMFEDGKSA SIDLLLILDP LEERTQMILS 910 920 930 940 950 960 LVEQFRPLKF VNIQVILMPT LELNIVPIRR IYVDDADIVK SITSEDSRSD PEVDIEMDVP 970 980 990 1000 1010 1020 NSFIVDNNYR IKKLLIELHS FSSKTVLSTG NIDGMGGVCL ALVDSAGNII DKTTTMKTFG 1030 1040 1050 1060 1070 1080 YGQFHTDKFL KGCYIKSCDS RYTVQSFSTD GHPDFIPSDS LDILSYNPQK IAVKISEEPT 1090 1100 1110 1120 1130 1140 HEEEYEEGRN NDTIINIFTI LESGPDEEER YMQMILSILS KCPETQKVNF FILDQPFISD 1150 1160 1170 1180 1190 1200 TLRKSCEYIN SSDEMRGNVI FLNYEWPQWL RPQRFSSRRR DVSRFLFLDV LLPQNISKVL 1210 1220 1230 1240 1250 1260 YMSPTEVPLD PFDIFQFQGL KRAPLGLFRM SGDGYWKEGY WEKMLRENNL EFYSTEPAFL 1270 1280 1290 1300 1310 1320 VNLERFRELD AGDKYRIHYQ RISTDAMSLV NIGQDLVNNL QLEVPIRFLK GSYKKKLVIN 1330 1340 1350 1360 DECVSEWKKK INKFASSPGD EDVPGESVSS KYQDSDNAAP LHDEL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The yeast KRE5 gene encodes a probable endoplasmic reticulum protein required for (1-->6)-beta-D-glucan synthesis and normal cell growth." Meaden P., Hill K., Wagner J., Slipetz D., Sommer S.S., Bussey H. Mol. Cell. Biol. 10:3013-3019(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Sequence of 29 kb around the PDR10 locus on the right arm of Saccharomyces cerevisiae chromosome XV: similarity to part of chromosome I." Parle-McDermott A.G., Hand N.J., Goulding S.E., Wolfe K.H. Yeast 12:999-1004(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XV." Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D. , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K. Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M33556 Genomic DNA. Translation: AAA34725.1. Z49821 Genomic DNA. Translation: CAA89981.1. Z75244 Genomic DNA. Translation: CAA99659.1. BK006948 Genomic DNA. Translation: DAA11097.1.
PIR	BVBYK5. S62066.
RefSeq	NP_014981.1. NM_001183756.1.
3D structure databases
ProteinModelPortal	P22023.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-5326N.
MINT	MINT-510348.
STRING	4932.YOR336W.
Protein family/group databases
CAZy	GT24. Glycosyltransferase Family 24.
Proteomic databases
PaxDb	P22023.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YOR336W; YOR336W; YOR336W.
GeneID	854514.
KEGG	sce:YOR336W.
Organism-specific databases
CYGD	YOR336w.
SGD	S000005863. KRE5.
Phylogenomic databases
eggNOG	NOG320899.
GeneTree	ENSGT00390000004600.
HOGENOM	HOG000000800.
KO	K11718.
OMA	WLRPQRF.
OrthoDB	EOG479JG7.
Gene expression databases
Genevestigator	P22023.
GermOnline	YOR336W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR009448. UDP-g_GGtrans. [Graphical view]
PANTHER	PTHR11226. PTHR11226. 1 hit.
Pfam	PF06427. UDP-g_GGTase. 1 hit. [Graphical view]
PROSITE	PS00014. ER_TARGET. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	976873.

Entry information

Entry name

KRE5_YEAST

Accession

Primary (citable) accession number: P22023
Secondary accession number(s): D6W331, Q12190

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1991
Last sequence update:	November 1, 1997
Last modified:	April 3, 2013
This is version 106 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program