P22012 (TBB_ASPFL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Tubulin beta chain Alternative name(s): Beta-tubulin
Organism	Aspergillus flavus
Taxonomic identifier	5059 [NCBI]
Taxonomic lineage	Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › mitosporic Trichocomaceae › Aspergillus

Protein attributes

Sequence length	448 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.
Subunit structure	Dimer of alpha and beta chains.
Subcellular location	Cytoplasm › cytoskeleton.
Sequence similarities	Belongs to the tubulin family.

Ontologies

Keywords
Cellular component	Cytoplasm Cytoskeleton Microtubule
Ligand	GTP-binding Nucleotide-binding
Gene Ontology (GO)
Biological process	microtubule-based movement Inferred from electronic annotation. Source: InterPro protein polymerization Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW microtubule Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW GTPase activity Inferred from electronic annotation. Source: InterPro structural molecule activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 448	448	Tubulin beta chain		PRO_0000048393
Regions
	Nucleotide binding	140 – 146	7	GTP Potential

Sequences

	Sequence		Length	Mass (Da)
	P22012 [UniParc]. Last modified August 1, 1991. Version 1. Checksum: 6E9FF3AF9FAB3EB4	FASTA	448	50,072
	10 20 30 40 50 60 MREIVHLQTG QCGNQIGAAF WQTISGEHGL DGSGVYNGSS DLQLERMNVY FNEASGNKYV 70 80 90 100 110 120 PRAVLVDLEP GTMDAVRAGP FGQLFRPDNF VFGQSGAGNN WAKGHYTEGA ELVDQVVDVV 130 140 150 160 170 180 RREAEGCDCL QGFQITHSLG GGTGAGMGTL LISKIREEFP DRMMATYSVV PSPKVSDTVV 190 200 210 220 230 240 EPYNATLSVH QLVEHSDETF CIDNEALYDI CMRTLKLSNP SYGDLNHLVS AVMSGVTTCL 250 260 270 280 290 300 RFPGQLNSDL RKLAVNMVPF PRLHFFMVGF APLTSRGAHS FRAVSVPELT QQMFDPKNMM 310 320 330 340 350 360 AASDFRNGRY LTCSAIFRGK VSMKEVEDQM RNIQSKNQTY FVEWIPNNIQ TALCSIPPRG 370 380 390 400 410 420 LKMSSTFIGN STSIQELFKR VGDQFTAMFR RKAFLHWYTG EGMDEMEFTE AESNMNDLVS 430 440 EYQQYQDASI SEGEEEYLEE EEPLEHEE

References

[1]	"Isolation and sequence analysis of a beta-tubulin gene from Aspergillus flavus and its use as a selectable marker." Seip E., Woloshuk C., Payne G., Curtis S. Appl. Environ. Microbiol. 56:3686-3692(1990) [PubMed: 2128007] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M38265 Genomic DNA. Translation: AAA32689.1.
PIR	A43794.
3D structure databases
ProteinModelPortal	P22012.
SMR	P22012. Positions 1-427.
ModBase	Search...
Protein-protein interaction databases
STRING	P22012.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR013838. Beta-tubulin_BS. IPR002453. Beta_tubulin. IPR008280. Tub_FtsZ_C. IPR000217. Tubulin. IPR018316. Tubulin/FtsZ_2-layer-sand-dom. IPR023123. Tubulin_C. IPR017975. Tubulin_CS. IPR003008. Tubulin_FtsZ_GTPase. [Graphical view]
Gene3D	G3DSA:3.30.1330.20. Tubulin/FtsZ_2-layer-sand-dom. 1 hit. G3DSA:1.10.287.600. Tubulin_C. 1 hit. G3DSA:3.40.50.1440. Tubulin_FtsZ. 1 hit.
Pfam	PF00091. Tubulin. 1 hit. PF03953. Tubulin_C. 1 hit. [Graphical view]
PRINTS	PR01163. BETATUBULIN. PR01161. TUBULIN.
SMART	SM00864. Tubulin. 1 hit. SM00865. Tubulin_C. 1 hit. [Graphical view]
SUPFAM	SSF55307. Tub_FtsZ_C. 1 hit. SSF52490. Tubulin_FtsZ. 1 hit.
PROSITE	PS00227. TUBULIN. 1 hit. PS00228. TUBULIN_B_AUTOREG. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TBB_ASPFL

Accession

Primary (citable) accession number: P22012

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1991
Last sequence update:	August 1, 1991
Last modified:	July 27, 2011
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program