Reviewed, UniProtKB/Swiss-Prot P22008 (P5CR_PSEAE)

Names and origin

Protein names

Recommended name:
    Pyrroline-5-carboxylate reductase
      Short name=P5C reductase
      Short name=P5CR
    EC=1.5.1.2

Gene names

Name:	proC
Ordered Locus Names:	PA0393

Organism

Pseudomonas aeruginosa [Complete proteome] [HAMAP]

Taxonomic identifier

287 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	273 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	L-proline + NAD(P)+ = 1-pyrroline-5-carboxylate + NAD(P)H.
Pathway	Amino-acid biosynthesis; L-proline biosynthesis; L-proline from L-glutamate 5-semialdehyde: step 1/1.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the pyrroline-5-carboxylate reductase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Proline biosynthesis
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW proline biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	binding Inferred from electronic annotation. Source: InterPro pyrroline-5-carboxylate reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.1
	Chain	2 – 273	272	Pyrroline-5-carboxylate reductase		PRO_0000187296

Sequences

	Sequence		Length	Mass (Da)
	P22008-1 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 6B2D191635E8DEE0	FASTA	273	28,094
	10 20 30 40 50 60 MSTPRIAFIG AGNMAASLIG GLRAQGVPAA QIRASDPGAE QRAKIAGEFA IDVVESNAEA 70 80 90 100 110 120 VADADVVVLS VKPQAMKAVC QALAPALKPE QLIVSIAAGI PCASLEAWLG QPRPVVRCMP 130 140 150 160 170 180 NTPALLRQGA SGLYANAQVS AAQCEQAGQL LSAVGIALWL DDEAQIDAVT AVSGSGPAYF 190 200 210 220 230 240 FLLMQAMTDA GEKLGLSRET ASRLTLQTAL GAAQMALSSE VEPAELRRRV TSPNGTTEAA 250 260 270 IKSFQANGFE ALVEQALNAA SQRSAELAEQ LGQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Comparison of proC and other housekeeping genes of Pseudomonas aeruginosa with their counterparts in Escherichia coli." Savioz A., Jeenes D.J., Kocher H.P., Haas D. Gene 86:107-111(1990) [PubMed: 2107123] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-13. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
[2]	"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen." Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R. , Kas A., Larbig K., Lim R.M., Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V. Nature 406:959-964(2000) [PubMed: 10984043] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
[3]	"Characterisation of a Pseudomonas aeruginosa twitching motility gene and evidence for a specialised protein export system widespread in eubacteria." Whitchurch C.B., Hobbs M., Livingston S.P., Krishnapillai V., Mattick J.S. Gene 101:33-44(1991) [PubMed: 1676385] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-7. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M33557 Genomic DNA. Translation: AAA25975.1. AE004091 Genomic DNA. Translation: AAG03782.1. M55524 Genomic DNA. Translation: AAA25958.1.
PIR	JQ0418.
RefSeq	NP_249084.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	878413.
GenomeReviews	Gene locus PA0393 in contig AE004091_GR.
KEGG	pae:PA0393.
NMPDR	fig|208964.1.peg.394.
Organism-specific databases
PseudoCAP	PA0393.
CMR	Search...
Phylogenomic databases
HOGENOM	P22008.
OMA	LKQGYPA.
Enzyme and pathway databases
BioCyc	PAER208964:PA0393-MON.
BRENDA	1.5.1.2. 354.
Family and domain databases
InterPro	IPR016040. NAD(P)-bd_dom. IPR004455. NADP_OxRdtase_F420. IPR000304. P5CR. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR11645. P5CR. 1 hit.
Pfam	PF03807. F420_oxidored. 1 hit. [Graphical view]
PIRSF	PIRSF000193. Pyrrol-5-carb_rd. 1 hit.
TIGRFAMs	TIGR00112. proC. 1 hit.
PROSITE	PS00521. P5CR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

P5CR_PSEAE

Accession

Primary (citable) accession number: P22008

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1991
Last sequence update:	January 23, 2007
Last modified:	November 3, 2009
This is version 69 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)